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This content will become publicly available on September 28, 2018

Title: Natural polyreactive IgA antibodies coat the intestinal microbiota

Large quantities of immunoglobulin A (IgA) are constitutively secreted by intestinal plasma cells to coat and contain the commensal microbiota, yet the specificity of these antibodies remains elusive. In this paper, we profiled the reactivities of single murine IgA plasma cells by cloning and characterizing large numbers of monoclonal antibodies. IgAs were not specific to individual bacterial taxa but rather polyreactive, with broad reactivity to a diverse, but defined, subset of microbiota. These antibodies arose at low frequencies among naïve B cells and were selected into the IgA repertoire upon recirculation in Peyer’s patches. This selection process occurred independent of microbiota or dietary antigens. Furthermore, although some IgAs acquired somatic mutations, these did not substantially influence their reactivity. In conclusion, these findings reveal an endogenous mechanism driving homeostatic production of polyreactive IgAs with innate specificity to microbiota.
Authors:
ORCiD logo [1] ;  [1] ; ORCiD logo [2] ;  [3] ;  [2] ;  [3] ; ORCiD logo [3] ; ORCiD logo [4] ; ORCiD logo [3] ; ORCiD logo [1]
  1. Univ. of Chicago, IL (United States). Committee on Immunology and Department of Pathology
  2. Argonne National Lab. (ANL), Argonne, IL (United States). Biosciences Division
  3. Univ. of Chicago, IL (United States). Committee on Immunology and Department of Medicine
  4. Argonne National Lab. (ANL), Argonne, IL (United States). Biosciences Division; Univ. of Chicago, IL (United States). Department of Medicine and Institute for Genomics and Systems Biology
Publication Date:
Grant/Contract Number:
AC02-06CH11357
Type:
Accepted Manuscript
Journal Name:
Science
Additional Journal Information:
Journal Volume: 358; Journal Issue: 6361; Journal ID: ISSN 0036-8075
Publisher:
AAAS
Research Org:
Argonne National Lab. (ANL), Argonne, IL (United States)
Sponsoring Org:
USDOE; National Institutes of Health (NIH)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES
OSTI Identifier:
1425279