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This content will become publicly available on October 7, 2018

Title: A power compensated differential scanning calorimeter for protein stability characterization

This study presented a power compensated MEMS differential scanning calorimeter (DSC) for protein stability characterization. In this microfabricated sensor, PDMS (Polydimethylsiloxane) and polyimide were used to construct the adiabatic chamber (1 μL) and temperature sensitive vanadium oxide was used as the thermistor material. A power compensation system was implemented to maintain the sample and reference at the same temperature. The resolution study and step response characterization indicated the high sensitivity (6 V/W) and low noise level (60 μk) of the device. The test with IgG1 antibody (mAb1) samples showed clear phase transitions and the data was confirmed to be reasonable by comparing it with the results of commercial DSC’s test. Finally, this device used ~1uL sample amount and could complete the scanning process in 4 min, significantly increasing the throughput of the bimolecular thermodynamics study like drug formulation process.
Authors:
 [1] ;  [2] ;  [3] ;  [4] ;  [4] ;  [5] ;  [2]
  1. Stony Brook Univ., NY (United States). Dept. of Mechanical Engineering
  2. Virginia Polytechnic Inst. and State Univ. (Virginia Tech), Blacksburg, VA (United States). Dept. of Mechanical Engineering
  3. AbbVie, Deutschland, Ludwigshafen (Germany)
  4. AbbVie Bioresearch Center, Worcester, MA (United States)
  5. Brookhaven National Lab. (BNL), Upton, NY (United States). Center for Functional Nanomaterials
Publication Date:
Report Number(s):
BNL-203256-2018-JAAM
Journal ID: ISSN 0925-4005
Grant/Contract Number:
SC0012704; IDBR 1152415
Type:
Accepted Manuscript
Journal Name:
Sensors and Actuators. B, Chemical
Additional Journal Information:
Journal Volume: 256; Journal ID: ISSN 0925-4005
Publisher:
Elsevier
Research Org:
Brookhaven National Lab. (BNL), Upton, NY (United States); Virginia Polytechnic Inst. and State Univ. (Virginia Tech), Blacksburg, VA (United States); AbbVie Bioresearch Center, Worcester, MA (United States)
Sponsoring Org:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22); National Science Foundation (NSF); AbbVie Inc. (United States)
Country of Publication:
United States
Language:
English
Subject:
36 MATERIALS SCIENCE; 47 OTHER INSTRUMENTATION; microcalorimeter; protein stability; differential scanning calorimeter
OSTI Identifier:
1425084