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Title: Abiotic Stresses Shift Belowground Populus-Associated Bacteria Toward a Core Stress Microbiome

Abstract

Adverse growth conditions can lead to decreased plant growth, productivity, and survival, resulting in poor yields or failure of crops and biofeedstocks. In some cases, the microbial community associated with plants has been shown to alleviate plant stress and increase plant growth under suboptimal growing conditions. A systematic understanding of how the microbial community changes under these conditions is required to understand the contribution of the microbiome to water utilization, nutrient uptake, and ultimately yield. Using a microbiome inoculation strategy, we studied how the belowground microbiome of Populus deltoides changes in response to diverse environmental conditions, including water limitation, light limitation (shading), and metal toxicity. While plant responses to treatments in terms of growth, photosynthesis, gene expression and metabolite profiles were varied, we identified a core set of bacterial genera that change in abundance in response to host stress. The results of this study indicate substantial structure in the plant microbiome community and identify potential drivers of the phytobiome response to stress. The identification of a common “stress microbiome” indicates tightly controlled relationships between the plant host and bacterial associates and a conserved structure in bacterial communities associated with poplar trees under different growth conditions. The ability of the microbiomemore » to buffer the plant from extreme environmental conditions coupled with the conserved stress microbiome observed in this study suggests an opportunity for future efforts aimed at predictably modulating the microbiome to optimize plant growth.« less

Authors:
ORCiD logo [1];  [2];  [3];  [4];  [3];  [3];  [3];  [3];  [4];  [3];  [3];  [3];  [3]; ORCiD logo [3];  [3];  [3];  [3];
+ Show Author Affiliations
  1. Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA, Joint Institute for Biological Sciences, University of Tennessee, Knoxville, Tennessee, USA
  2. School of Forest Resources and Environmental Science, Michigan Technological University, Houghton, Michigan, USA
  3. Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA
  4. Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA, University of Arkansas for Medical Sciences, Little Rock, Arkansas, USA
Publication Date:
Research Org.:
Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER)
OSTI Identifier:
1461238
Alternate Identifier(s):
OSTI ID: 1424444
Grant/Contract Number:  
AC05-00OR22725
Resource Type:
Published Article
Journal Name:
mSystems
Additional Journal Information:
Journal Name: mSystems Journal Volume: 3 Journal Issue: 1; Journal ID: ISSN 2379-5077
Publisher:
American Society for Microbiology
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; drought; microbiome; poplar; shading

Citation Formats

Timm, Collin M., Carter, Kelsey R., Carrell, Alyssa A., Jun, Se-Ran, Jawdy, Sara S., Vélez, Jessica M., Gunter, Lee E., Yang, Zamin, Nookaew, Intawat, Engle, Nancy L., Lu, Tse-Yuan S., Schadt, Christopher W., Tschaplinski, Timothy J., Doktycz, Mitchel J., Tuskan, Gerald A., Pelletier, Dale A., Weston, David J., and Herr, ed., Joshua R. Abiotic Stresses Shift Belowground Populus-Associated Bacteria Toward a Core Stress Microbiome. United States: N. p., 2018. Web. doi:10.1128/mSystems.00070-17.
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Timm, Collin M., Carter, Kelsey R., Carrell, Alyssa A., Jun, Se-Ran, Jawdy, Sara S., Vélez, Jessica M., Gunter, Lee E., Yang, Zamin, Nookaew, Intawat, Engle, Nancy L., Lu, Tse-Yuan S., Schadt, Christopher W., Tschaplinski, Timothy J., Doktycz, Mitchel J., Tuskan, Gerald A., Pelletier, Dale A., Weston, David J., & Herr, ed., Joshua R. Abiotic Stresses Shift Belowground Populus-Associated Bacteria Toward a Core Stress Microbiome. United States. https://doi.org/10.1128/mSystems.00070-17
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Timm, Collin M., Carter, Kelsey R., Carrell, Alyssa A., Jun, Se-Ran, Jawdy, Sara S., Vélez, Jessica M., Gunter, Lee E., Yang, Zamin, Nookaew, Intawat, Engle, Nancy L., Lu, Tse-Yuan S., Schadt, Christopher W., Tschaplinski, Timothy J., Doktycz, Mitchel J., Tuskan, Gerald A., Pelletier, Dale A., Weston, David J., and Herr, ed., Joshua R. Tue . "Abiotic Stresses Shift Belowground Populus-Associated Bacteria Toward a Core Stress Microbiome". United States. https://doi.org/10.1128/mSystems.00070-17.
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@article{osti_1461238,
title = {Abiotic Stresses Shift Belowground Populus-Associated Bacteria Toward a Core Stress Microbiome},
author = {Timm, Collin M. and Carter, Kelsey R. and Carrell, Alyssa A. and Jun, Se-Ran and Jawdy, Sara S. and Vélez, Jessica M. and Gunter, Lee E. and Yang, Zamin and Nookaew, Intawat and Engle, Nancy L. and Lu, Tse-Yuan S. and Schadt, Christopher W. and Tschaplinski, Timothy J. and Doktycz, Mitchel J. and Tuskan, Gerald A. and Pelletier, Dale A. and Weston, David J. and Herr, ed., Joshua R.},
abstractNote = {Adverse growth conditions can lead to decreased plant growth, productivity, and survival, resulting in poor yields or failure of crops and biofeedstocks. In some cases, the microbial community associated with plants has been shown to alleviate plant stress and increase plant growth under suboptimal growing conditions. A systematic understanding of how the microbial community changes under these conditions is required to understand the contribution of the microbiome to water utilization, nutrient uptake, and ultimately yield. Using a microbiome inoculation strategy, we studied how the belowground microbiome of Populus deltoides changes in response to diverse environmental conditions, including water limitation, light limitation (shading), and metal toxicity. While plant responses to treatments in terms of growth, photosynthesis, gene expression and metabolite profiles were varied, we identified a core set of bacterial genera that change in abundance in response to host stress. The results of this study indicate substantial structure in the plant microbiome community and identify potential drivers of the phytobiome response to stress. The identification of a common “stress microbiome” indicates tightly controlled relationships between the plant host and bacterial associates and a conserved structure in bacterial communities associated with poplar trees under different growth conditions. The ability of the microbiome to buffer the plant from extreme environmental conditions coupled with the conserved stress microbiome observed in this study suggests an opportunity for future efforts aimed at predictably modulating the microbiome to optimize plant growth.},
doi = {10.1128/mSystems.00070-17},
journal = {mSystems},
number = 1,
volume = 3,
place = {United States},
year = {Tue Jan 23 00:00:00 EST 2018},
month = {Tue Jan 23 00:00:00 EST 2018}
}
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https://doi.org/10.1128/mSystems.00070-17
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