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Title: The Arabidopsis MTP8 transporter determines the localization of manganese and iron in seeds

Abstract

Understanding how seeds obtain and store nutrients is key to developing crops with higher agronomic and nutritional value. We have uncovered unique patterns of micronutrient localization in seeds using synchrotron X-ray fluorescence (SXRF). Although all four members of the Arabidopsis thaliana Mn-CDF family can transport Mn, here we show that only mtp8-2 has an altered Mn distribution pattern in seeds. In an mtp8-2 mutant, Mn no longer accumulates in hypocotyl cortex cells and sub-epidermal cells of the embryonic cotyledons, but rather accumulates with Fe in the cells surrounding the vasculature, a pattern previously shown to be determined by the vacuolar transporter VIT1. We also show that MTP8, unlike the other three Mn-CDF family members, can transport Fe and is responsible for localization of Fe to the same cells that store Mn. When both the VIT1 and MTP8 transporters are non-functional, there is no accumulation of Fe or Mn in specific cell types; rather these elements are distributed amongst all cell types in the seed. Finally, disruption of the putative Fe binding sites in MTP8 resulted in loss of ability to transport Fe but did not affect the ability to transport Mn.

Authors:
 [1];  [1];  [1];  [1];  [1];  [1]
  1. Dartmouth College, Hanover, NH (United States). Dept. of Biological Sciences
Publication Date:
Research Org.:
Univ. of Chicago, IL (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
OSTI Identifier:
1424223
Grant/Contract Number:  
FG02-94ER14466; AC02-06CH11357
Resource Type:
Accepted Manuscript
Journal Name:
Scientific Reports
Additional Journal Information:
Journal Volume: 7; Journal Issue: 1; Journal ID: ISSN 2045-2322
Publisher:
Nature Publishing Group
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Abiotic; Plant genetics

Citation Formats

Chu, Heng-Hsuan, Car, Suzana, Socha, Amanda L., Hindt, Maria N., Punshon, Tracy, and Guerinot, Mary Lou. The Arabidopsis MTP8 transporter determines the localization of manganese and iron in seeds. United States: N. p., 2017. Web. doi:10.1038/s41598-017-11250-9.
Chu, Heng-Hsuan, Car, Suzana, Socha, Amanda L., Hindt, Maria N., Punshon, Tracy, & Guerinot, Mary Lou. The Arabidopsis MTP8 transporter determines the localization of manganese and iron in seeds. United States. doi:10.1038/s41598-017-11250-9.
Chu, Heng-Hsuan, Car, Suzana, Socha, Amanda L., Hindt, Maria N., Punshon, Tracy, and Guerinot, Mary Lou. Fri . "The Arabidopsis MTP8 transporter determines the localization of manganese and iron in seeds". United States. doi:10.1038/s41598-017-11250-9. https://www.osti.gov/servlets/purl/1424223.
@article{osti_1424223,
title = {The Arabidopsis MTP8 transporter determines the localization of manganese and iron in seeds},
author = {Chu, Heng-Hsuan and Car, Suzana and Socha, Amanda L. and Hindt, Maria N. and Punshon, Tracy and Guerinot, Mary Lou},
abstractNote = {Understanding how seeds obtain and store nutrients is key to developing crops with higher agronomic and nutritional value. We have uncovered unique patterns of micronutrient localization in seeds using synchrotron X-ray fluorescence (SXRF). Although all four members of the Arabidopsis thaliana Mn-CDF family can transport Mn, here we show that only mtp8-2 has an altered Mn distribution pattern in seeds. In an mtp8-2 mutant, Mn no longer accumulates in hypocotyl cortex cells and sub-epidermal cells of the embryonic cotyledons, but rather accumulates with Fe in the cells surrounding the vasculature, a pattern previously shown to be determined by the vacuolar transporter VIT1. We also show that MTP8, unlike the other three Mn-CDF family members, can transport Fe and is responsible for localization of Fe to the same cells that store Mn. When both the VIT1 and MTP8 transporters are non-functional, there is no accumulation of Fe or Mn in specific cell types; rather these elements are distributed amongst all cell types in the seed. Finally, disruption of the putative Fe binding sites in MTP8 resulted in loss of ability to transport Fe but did not affect the ability to transport Mn.},
doi = {10.1038/s41598-017-11250-9},
journal = {Scientific Reports},
number = 1,
volume = 7,
place = {United States},
year = {2017},
month = {9}
}

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    Works referencing / citing this record:

    Essential and Detrimental — an Update on Intracellular Iron Trafficking and Homeostasis
    journal, May 2019

    • Vigani, Gianpiero; Solti, �d�m; Thomine, S�bastien
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