Title: Tubulin C-terminal Post-translational Modifications Do Not Occur in Wood Forming Tissue of Populus

Abstract

Cortical microtubules (MTs) are evolutionarily conserved cytoskeletal components with specialized roles in plants, including regulation of cell wall biogenesis. MT functions and dynamics are dictated by the composition of their monomeric subunits, α- (TUA) and β-tubulins (TUB), which in animals and protists are subject to both transcriptional regulation and post-translational modifications (PTM). While spatiotemporal regulation of tubulin gene expression has been reported in plants, whether and to what extent tubulin PTMs occur in these species remain poorly understood. We chose the woody perennial Populus for investigation of tubulin PTMs in this study, with a particular focus on developing xylem where high tubulin transcript levels support MT-dependent secondary cell wall deposition. Mass spectrometry and immunodetection concurred that detyrosination, non-tyrosination and glutamylation were essentially absent in tubulins isolated from wood-forming tissues of P. deltoides and P. tremula ×alba. Label-free quantification of tubulin isotypes and RNA-Seq estimation of tubulin transcript abundance were largely consistent with transcriptional regulation. However, two TUB isotypes were detected at noticeably lower levels than expected based on RNA-Seq transcript abundance in both Populus species. These findings led us to conclude that MT composition during wood formation depends exclusively on transcriptional and, to a lesser extent, translational regulation of tubulinmore » isotypes.« less

Authors:

Hu, Hao ^[1]; Gu, Xi ^[1]; Xue, Liang-Jiao ^[1]; Swamy, Prashant S. ^[1]; Harding, Scott A. ^[1]; Tsai, Chung-Jui ^[1]

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+ Show Author Affiliations

1. Univ. of Georgia, Athens, GA (United States)

Publication Date:

2016-10-13

Research Org.:

Univ. of Georgia, Athens, GA (United States)

Sponsoring Org.:

USDOE Office of Science (SC), Biological and Environmental Research (BER)

OSTI Identifier:

1423767

Grant/Contract Number:  

SC0008470

Resource Type:

Accepted Manuscript

Journal Name:

Frontiers in Plant Science

Additional Journal Information:

Journal Volume: 7; Journal ID: ISSN 1664-462X

Publisher:

Frontiers Research Foundation

Country of Publication:

United States

Language:

English

Subject:

54 ENVIRONMENTAL SCIENCES