High-fidelity detection of crop biomass quantitative trait loci from low-cost imaging in the field

Banan, Darshi; Paul, Rachel E.; Feldman, Max J.; Holmes, Mark W.; Schlake, Hannah; Baxter, Ivan; Jiang, Hui; Leakey, Andrew D. B.

doi:10.1002/pld3.41

Title: High-fidelity detection of crop biomass quantitative trait loci from low-cost imaging in the field

Journal Article · 22 February 2018 · Plant Direct

DOI: https://doi.org/10.1002/pld3.41 · OSTI ID:1423165

Banan, Darshi ^[1]; Paul, Rachel E. ^[1]; Feldman, Max J. ^[2]; Holmes, Mark W. ^[1]; Schlake, Hannah ^[1]; Baxter, Ivan ^[3]; Jiang, Hui ^[2]; Leakey, Andrew D. B. ^[1]

Univ. of Illinois, Urbana-Champaign, IL (United States)
Donald Danforth Plant Science Center, St. Louis, MO (United States)
US Dept. of Agriculture (USDA)., St. Louis, MO (United States). Donald Danforth Plant Science Center

Field-based, rapid, and nondestructive techniques for assessing plant productivity are needed to accelerate the discovery of genotype-to-phenotype relationships in next-generation biomass grass crops. The use of hemispherical imaging and light attenuation modeling was evaluated against destructive harvest measures with respect to their ability to accurately capture phenotypic and genotypic relationships in a field-grown grass crop. Plant area index (PAI) estimated from below-canopy hemispherical images, as well as a suite of thirteen traits assessed by manual destructive harvests, were measured in a Setaria recombinant inbred line mapping population segregating for aboveground productivity and architecture. A significant correlation was observed between PAI and biomass production across the population at maturity (r² = .60), as well as for select diverse genotypes sampled repeatedly over the growing season (r² = .79). Twenty-seven quantitative trait loci (QTL) were detected for manually collected traits associated with biomass production. Of these, twenty-one were found in four clusters of colocalized QTL. Analysis of image-based estimates of PAI successfully identified all four QTL hot spots for biomass production. QTL for PAI had greater overlap with those detected for traits associated with biomass production than with those for plant architecture and biomass partitioning. Hemispherical imaging is an affordable and scalable method, which demonstrates how high-throughput phenotyping can identify QTL related to biomass production of field trials in place of destructive harvests that are labor, time, and material intensive.

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Research Organization:: Donald Danforth Plant Science Center, St. Louis, MO (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER); USDOE

Grant/Contract Number:: SC0018277; SC0008769; DE‐SC0008769

OSTI ID:: 1423165

Alternate ID(s):: OSTI ID: 1423169; OSTI ID: 1530865

Journal Information:: Plant Direct, Vol. 2, Issue 2; ISSN 2475-4455

Publisher:: Wiley and American Society of Plant Biologists and Society for Experimental BiologyCopyright Statement

Country of Publication:: United States

Language:: English

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Data from: High fidelity detection of crop biomass QTL from low-cost imaging in the field Banan, Darshi; Paul, Rachel E.; Feldman, Max J. Dryad https://doi.org/10.5061/dryad.d55j7	dataset	January 2019
Data from: High fidelity detection of crop biomass QTL from low-cost imaging in the field Banan, Darshi; Paul, Rachel E.; Feldman, Max J. Dryad https://doi.org/10.5061/dryad.d55j7	dataset	January 2019