Analyzing Xyloglucan Endotransglycosylases by Incorporating Synthetic Oligosaccharides into Plant Cell Walls
Abstract
Abstract The plant cell wall is a cellular exoskeleton consisting predominantly of a complex polysaccharide network that defines the shape of cells. During growth, this network can be loosened through the action of xyloglucan endotransglycosylases (XETs), glycoside hydrolases that “cut and paste” xyloglucan polysaccharides through a transglycosylation process. We have analyzed cohorts of XETs in different plant species to evaluate the substrate specificities of xyloglucan acceptors by using a set of synthetic oligosaccharides obtained by automated glycan assembly. The ability of XETs to incorporate the oligosaccharides into polysaccharides printed as microarrays and into stem sections of Arabidopsis thaliana , beans, and peas was assessed. We found that single xylose substitutions are sufficient for transfer, and xylosylation of the terminal glucose residue is not required by XETs, independent of plant species. To obtain information on the potential xylosylation pattern of the natural acceptor of XETs, that is, the nonreducing end of xyloglucan, we further tested the activity of xyloglucan xylosyl transferase (XXT) 2 on the synthetic xyloglucan oligosaccharides. These data shed light on inconsistencies between previous studies towards determining the acceptor substrate specificities of XETs and have important implications for further understanding plant cell wall polysaccharide synthesis and remodeling.
- Authors:
-
[1]
- Department of Biomolecular Systems Max Planck Institute of Colloids and Interfaces Am Mühlenberg 1 14476 Potsdam Germany, Freie Universität Berlin Institute of Chemistry and Biochemistry Arnimallee 22 14195 Berlin Germany
- Complex Carbohydrate Research Center University of Georgia 315 Riverbend Road Athens GA 30602 USA
- Publication Date:
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1422455
- Resource Type:
- Publisher's Accepted Manuscript
- Journal Name:
- ChemBioChem: a European journal of chemical biology
- Additional Journal Information:
- Journal Name: ChemBioChem: a European journal of chemical biology Journal Volume: 19 Journal Issue: 8; Journal ID: ISSN 1439-4227
- Publisher:
- Wiley Blackwell (John Wiley & Sons)
- Country of Publication:
- France
- Language:
- English
Citation Formats
Ruprecht, Colin, Dallabernardina, Pietro, Smith, Peter J., Urbanowicz, Breeanna R., and Pfrengle, Fabian. Analyzing Xyloglucan Endotransglycosylases by Incorporating Synthetic Oligosaccharides into Plant Cell Walls. France: N. p., 2018.
Web. doi:10.1002/cbic.201700638.
Ruprecht, Colin, Dallabernardina, Pietro, Smith, Peter J., Urbanowicz, Breeanna R., & Pfrengle, Fabian. Analyzing Xyloglucan Endotransglycosylases by Incorporating Synthetic Oligosaccharides into Plant Cell Walls. France. https://doi.org/10.1002/cbic.201700638
Ruprecht, Colin, Dallabernardina, Pietro, Smith, Peter J., Urbanowicz, Breeanna R., and Pfrengle, Fabian. Fri .
"Analyzing Xyloglucan Endotransglycosylases by Incorporating Synthetic Oligosaccharides into Plant Cell Walls". France. https://doi.org/10.1002/cbic.201700638.
@article{osti_1422455,
title = {Analyzing Xyloglucan Endotransglycosylases by Incorporating Synthetic Oligosaccharides into Plant Cell Walls},
author = {Ruprecht, Colin and Dallabernardina, Pietro and Smith, Peter J. and Urbanowicz, Breeanna R. and Pfrengle, Fabian},
abstractNote = {Abstract The plant cell wall is a cellular exoskeleton consisting predominantly of a complex polysaccharide network that defines the shape of cells. During growth, this network can be loosened through the action of xyloglucan endotransglycosylases (XETs), glycoside hydrolases that “cut and paste” xyloglucan polysaccharides through a transglycosylation process. We have analyzed cohorts of XETs in different plant species to evaluate the substrate specificities of xyloglucan acceptors by using a set of synthetic oligosaccharides obtained by automated glycan assembly. The ability of XETs to incorporate the oligosaccharides into polysaccharides printed as microarrays and into stem sections of Arabidopsis thaliana , beans, and peas was assessed. We found that single xylose substitutions are sufficient for transfer, and xylosylation of the terminal glucose residue is not required by XETs, independent of plant species. To obtain information on the potential xylosylation pattern of the natural acceptor of XETs, that is, the nonreducing end of xyloglucan, we further tested the activity of xyloglucan xylosyl transferase (XXT) 2 on the synthetic xyloglucan oligosaccharides. These data shed light on inconsistencies between previous studies towards determining the acceptor substrate specificities of XETs and have important implications for further understanding plant cell wall polysaccharide synthesis and remodeling.},
doi = {10.1002/cbic.201700638},
journal = {ChemBioChem: a European journal of chemical biology},
number = 8,
volume = 19,
place = {France},
year = {Fri Feb 23 00:00:00 EST 2018},
month = {Fri Feb 23 00:00:00 EST 2018}
}
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https://doi.org/10.1002/cbic.201700638
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