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Title: Droplet microfluidics for synthetic biology

Abstract

Here, synthetic biology is an interdisciplinary field that aims to engineer biological systems for useful purposes. Organism engineering often requires the optimization of individual genes and/or entire biological pathways (consisting of multiple genes). Advances in DNA sequencing and synthesis have recently begun to enable the possibility of evaluating thousands of gene variants and hundreds of thousands of gene combinations. However, such large-scale optimization experiments remain cost-prohibitive to researchers following traditional molecular biology practices, which are frequently labor-intensive and suffer from poor reproducibility. Liquid handling robotics may reduce labor and improve reproducibility, but are themselves expensive and thus inaccessible to most researchers. Microfluidic platforms offer a lower entry price point alternative to robotics, and maintain high throughput and reproducibility while further reducing operating costs through diminished reagent volume requirements. Droplet microfluidics have shown exceptional promise for synthetic biology experiments, including DNA assembly, transformation/transfection, culturing, cell sorting, phenotypic assays, artificial cells and genetic circuits.

Authors:
 [1];  [1];  [1];  [2]; ORCiD logo [1]
  1. DOE Joint BioEnergy Institute, Emeryville, CA (United States); Sandia National Lab. (SNL-CA), Livermore, CA (United States)
  2. DOE Joint BioEnergy Institute, Emeryville, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States)
Publication Date:
Research Org.:
Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1421856
Alternate Identifier(s):
OSTI ID: 1436333
Report Number(s):
SAND-2017-9052J
Journal ID: ISSN 1473-0197; LCAHAM; 656492
Grant/Contract Number:  
AC04-94AL85000; AC02-05CH11231
Resource Type:
Accepted Manuscript
Journal Name:
Lab on a chip (Print)
Additional Journal Information:
Journal Name: Lab on a chip (Print); Journal Volume: 17; Journal Issue: 20; Journal ID: ISSN 1473-0197
Publisher:
Royal Society of Chemistry
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Gach, Philip Charles, Iwai, Kosuke, Kim, Peter Wonhee, Hillson, Nathan J., and Singh, Anup K. Droplet microfluidics for synthetic biology. United States: N. p., 2017. Web. doi:10.1039/C7LC00576H.
Gach, Philip Charles, Iwai, Kosuke, Kim, Peter Wonhee, Hillson, Nathan J., & Singh, Anup K. Droplet microfluidics for synthetic biology. United States. doi:10.1039/C7LC00576H.
Gach, Philip Charles, Iwai, Kosuke, Kim, Peter Wonhee, Hillson, Nathan J., and Singh, Anup K. Thu . "Droplet microfluidics for synthetic biology". United States. doi:10.1039/C7LC00576H. https://www.osti.gov/servlets/purl/1421856.
@article{osti_1421856,
title = {Droplet microfluidics for synthetic biology},
author = {Gach, Philip Charles and Iwai, Kosuke and Kim, Peter Wonhee and Hillson, Nathan J. and Singh, Anup K.},
abstractNote = {Here, synthetic biology is an interdisciplinary field that aims to engineer biological systems for useful purposes. Organism engineering often requires the optimization of individual genes and/or entire biological pathways (consisting of multiple genes). Advances in DNA sequencing and synthesis have recently begun to enable the possibility of evaluating thousands of gene variants and hundreds of thousands of gene combinations. However, such large-scale optimization experiments remain cost-prohibitive to researchers following traditional molecular biology practices, which are frequently labor-intensive and suffer from poor reproducibility. Liquid handling robotics may reduce labor and improve reproducibility, but are themselves expensive and thus inaccessible to most researchers. Microfluidic platforms offer a lower entry price point alternative to robotics, and maintain high throughput and reproducibility while further reducing operating costs through diminished reagent volume requirements. Droplet microfluidics have shown exceptional promise for synthetic biology experiments, including DNA assembly, transformation/transfection, culturing, cell sorting, phenotypic assays, artificial cells and genetic circuits.},
doi = {10.1039/C7LC00576H},
journal = {Lab on a chip (Print)},
number = 20,
volume = 17,
place = {United States},
year = {2017},
month = {8}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record

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Cited by: 12 works
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Figures / Tables:

Table 1 Table 1: Comparison of conventional and microfluidic approaches to synthetic biology

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  • Eyer, Klaus; Root, Katharina; Verboket, Pascal E.
  • Scientific Reports, Vol. 5, Issue 1
  • DOI: 10.1038/srep16551

A Versatile Microfluidic Device for Automating Synthetic Biology
journal, June 2015


Specific and Reversible DNA-Directed Self-Assembly of Modular Vesicle-Droplet Hybrid Materials
journal, March 2016


Integrated Microfluidic System for Size-Based Selection and Trapping of Giant Vesicles
journal, December 2015


Fluid Transport in Partially Shielded Electrowetting on Dielectric Digital Microfluidic Devices
journal, August 2016

  • Madison, Andrew C.; Royal, Matthew W.; Fair, Richard B.
  • Journal of Microelectromechanical Systems, Vol. 25, Issue 4
  • DOI: 10.1109/JMEMS.2016.2561699

A One Pot, One Step, Precision Cloning Method with High Throughput Capability
journal, November 2008


High-throughput and combinatorial gene expression on a chip for metabolism-induced toxicology screening
journal, May 2014

  • Kwon, Seok Joon; Lee, Dong Woo; Shah, Dhiral A.
  • Nature Communications, Vol. 5, Issue 1
  • DOI: 10.1038/ncomms4739

    Works referencing / citing this record:

    A flexible precise volume sensor based on metal-on-polyimide electrodes sandwiched by PDMS channel for microfluidic systems
    journal, November 2019


    Antifouling digital microfluidics using lubricant infused porous film
    journal, January 2019


    A flexible precise volume sensor based on metal-on-polyimide electrodes sandwiched by PDMS channel for microfluidic systems
    journal, November 2019


    Antifouling digital microfluidics using lubricant infused porous film
    journal, January 2019


      Figures / Tables found in this record:

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