Droplet microfluidics for synthetic biology
Abstract
Review of current droplet microfluidics systems as they apply to the field of synthetic biology and genetic engineering.
- Authors:
-
- DOE Joint BioEnergy Institute, Emeryville, CA (United States); Sandia National Lab. (SNL-CA), Livermore, CA (United States)
- DOE Joint BioEnergy Institute, Emeryville, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States)
- Publication Date:
- Research Org.:
- Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- OSTI Identifier:
- 1421856
- Alternate Identifier(s):
- OSTI ID: 1436333
- Report Number(s):
- SAND-2017-9052J
Journal ID: ISSN 1473-0197; LCAHAM; 656492
- Grant/Contract Number:
- AC04-94AL85000; AC02-05CH11231
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Lab on a chip (Print)
- Additional Journal Information:
- Journal Name: Lab on a chip (Print); Journal Volume: 17; Journal Issue: 20; Journal ID: ISSN 1473-0197
- Publisher:
- Royal Society of Chemistry
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Gach, Philip Charles, Iwai, Kosuke, Kim, Peter Wonhee, Hillson, Nathan J., and Singh, Anup K. Droplet microfluidics for synthetic biology. United States: N. p., 2017.
Web. doi:10.1039/C7LC00576H.
Gach, Philip Charles, Iwai, Kosuke, Kim, Peter Wonhee, Hillson, Nathan J., & Singh, Anup K. Droplet microfluidics for synthetic biology. United States. https://doi.org/10.1039/C7LC00576H
Gach, Philip Charles, Iwai, Kosuke, Kim, Peter Wonhee, Hillson, Nathan J., and Singh, Anup K. Thu .
"Droplet microfluidics for synthetic biology". United States. https://doi.org/10.1039/C7LC00576H. https://www.osti.gov/servlets/purl/1421856.
@article{osti_1421856,
title = {Droplet microfluidics for synthetic biology},
author = {Gach, Philip Charles and Iwai, Kosuke and Kim, Peter Wonhee and Hillson, Nathan J. and Singh, Anup K.},
abstractNote = {Review of current droplet microfluidics systems as they apply to the field of synthetic biology and genetic engineering.},
doi = {10.1039/C7LC00576H},
journal = {Lab on a chip (Print)},
number = 20,
volume = 17,
place = {United States},
year = {Thu Aug 10 00:00:00 EDT 2017},
month = {Thu Aug 10 00:00:00 EDT 2017}
}
Free Publicly Available Full Text
Publisher's Version of Record
Other availability
Cited by: 68 works
Citation information provided by
Web of Science
Web of Science
Figures / Tables:
Table 1: Comparison of conventional and microfluidic approaches to synthetic biology
All figures and tables
(1 total)
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.
Works referenced in this record:
Large-scale de novo DNA synthesis: technologies and applications
journal, April 2014
- Kosuri, Sriram; Church, George M.
- Nature Methods, Vol. 11, Issue 5
High-Throughput Single-Cell Cultivation on Microfluidic Streak Plates
journal, February 2016
- Jiang, Cheng-Ying; Dong, Libing; Zhao, Jian-Kang
- Applied and Environmental Microbiology, Vol. 82, Issue 7
Sensitive, High Throughput Detection of Proteins in Individual, Surfactant-Stabilized Picoliter Droplets Using Nanoelectrospray Ionization Mass Spectrometry
journal, March 2013
- Smith, Clive A.; Li, Xin; Mize, Todd H.
- Analytical Chemistry, Vol. 85, Issue 8
Scalable Device for Automated Microbial Electroporation in a Digital Microfluidic Platform
journal, June 2017
- Madison, Andrew C.; Royal, Matthew W.; Vigneault, Frederic
- ACS Synthetic Biology, Vol. 6, Issue 9
Streamlining the Design-to-Build Transition with Build-Optimization Software Tools
journal, November 2016
- Oberortner, Ernst; Cheng, Jan-Fang; Hillson, Nathan J.
- ACS Synthetic Biology, Vol. 6, Issue 3
Heat-shock transformation of Escherichia coli in nanolitre droplets formed in a capillary-composited microfluidic device
journal, January 2011
- Sha, Jun; Wang, Yaolei; Wang, Jianchun
- Analytical Methods, Vol. 3, Issue 9
Interfacing Droplet Microfluidics with Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry: Label-Free Content Analysis of Single Droplets
journal, January 2013
- Küster, Simon K.; Fagerer, Stephan R.; Verboket, Pascal E.
- Analytical Chemistry, Vol. 85, Issue 3
Droplet-based microfluidics for artificial cell generation: a brief review
journal, August 2016
- Martino, Chiara; deMello, Andrew J.
- Interface Focus, Vol. 6, Issue 4
Attolitre-sized lipid bilayer chamber array for rapid detection of single transporters
journal, June 2015
- Soga, Naoki; Watanabe, Rikiya; Noji, Hiroyuki
- Scientific Reports, Vol. 5, Issue 1
Synthesis and cell-free cloning of DNA libraries using programmable microfluidics
journal, October 2015
- Yehezkel, Tuval Ben; Rival, Arnaud; Raz, Ofir
- Nucleic Acids Research, Vol. 44, Issue 4
Building droplet-based microfluidic systems for biological analysis
journal, July 2012
- Niu, Xize; deMello, Andrew J.
- Biochemical Society Transactions, Vol. 40, Issue 4
Pulse-density modulation control of chemical oscillation far from equilibrium in a droplet open-reactor system
journal, January 2016
- Sugiura, Haruka; Ito, Manami; Okuaki, Tomoya
- Nature Communications, Vol. 7, Issue 1
Single-cell analysis and sorting using droplet-based microfluidics
journal, April 2013
- Mazutis, Linas; Gilbert, John; Ung, W. Lloyd
- Nature Protocols, Vol. 8, Issue 5
The Poisson distribution and beyond: methods for microfluidic droplet production and single cell encapsulation
journal, January 2015
- Collins, David J.; Neild, Adrian; deMello, Andrew
- Lab on a Chip, Vol. 15, Issue 17
High-throughput screening of filamentous fungi using nanoliter-range droplet-based microfluidics
journal, June 2016
- Beneyton, Thomas; Wijaya, I. Putu Mahendra; Postros, Prexilia
- Scientific Reports, Vol. 6, Issue 1
Fluorinated Pickering Emulsions Impede Interfacial Transport and Form Rigid Interface for the Growth of Anchorage-Dependent Cells
journal, November 2014
- Pan, Ming; Rosenfeld, Liat; Kim, Minkyu
- ACS Applied Materials & Interfaces, Vol. 6, Issue 23
Digital Microfluidic Approach for Efficient Electroporation with High Productivity: Transgene Expression of Microalgae without Cell Wall Removal
journal, June 2015
- Im, Do Jin; Jeong, Su-Nam; Yoo, Byeong Sun
- Analytical Chemistry, Vol. 87, Issue 13
Capillary-composited microfluidic device for heat shock transformation of Escherichia coli
journal, October 2011
- Sha, Jun; Wang, Yaolei; Wang, Jianchun
- Journal of Bioscience and Bioengineering, Vol. 112, Issue 4
Synchronous long-term oscillations in a synthetic gene circuit
journal, October 2016
- Potvin-Trottier, Laurent; Lord, Nathan D.; Vinnicombe, Glenn
- Nature, Vol. 538, Issue 7626
Effective Optimization of Antibody Affinity by Phage Display Integrated with High-Throughput DNA Synthesis and Sequencing Technologies
journal, June 2015
- Hu, Dongmei; Hu, Siyi; Wan, Wen
- PLOS ONE, Vol. 10, Issue 6
Accurate microfluidic sorting of droplets at 30 kHz
journal, January 2015
- Sciambi, Adam; Abate, Adam R.
- Lab on a Chip, Vol. 15, Issue 1
Microfluidic droplet platform for ultrahigh-throughput single-cell screening of biodiversity
journal, February 2017
- Terekhov, Stanislav S.; Smirnov, Ivan V.; Stepanova, Anastasiya V.
- Proceedings of the National Academy of Sciences, Vol. 114, Issue 10
A microfluidic oligonucleotide synthesizer
journal, February 2010
- Lee, Cheng-Chung; Snyder, Thomas M.; Quake, Stephen R.
- Nucleic Acids Research, Vol. 38, Issue 8
Enzymatic assembly of DNA molecules up to several hundred kilobases
journal, April 2009
- Gibson, Daniel G.; Young, Lei; Chuang, Ray-Yuan
- Nature Methods, Vol. 6, Issue 5, p. 343-345
Mechanically activated artificial cell by using microfluidics
journal, September 2016
- Ho, Kenneth K. Y.; Lee, Lap Man; Liu, Allen P.
- Scientific Reports, Vol. 6, Issue 1
Entrainment and Control of Bacterial Populations: An in Silico Study over a Spatially Extended Agent Based Model
journal, April 2016
- Mina, Petros; Tsaneva-Atanasova, Krasimira; Bernardo, Mario di
- ACS Synthetic Biology, Vol. 5, Issue 7
Principles of genetic circuit design
journal, April 2014
- Brophy, Jennifer A. N.; Voigt, Christopher A.
- Nature Methods, Vol. 11, Issue 5
Continuous microfluidic fabrication of synthetic asymmetric vesicles
journal, January 2015
- Lu, Li; Schertzer, Jeffrey W.; Chiarot, Paul R.
- Lab on a Chip, Vol. 15, Issue 17
Marching along to an Offbeat Drum: Entrainment of Synthetic Gene Oscillators by a Noisy Stimulus
journal, November 2015
- Butzin, Nicholas C.; Hochendoner, Philip; Ogle, Curtis T.
- ACS Synthetic Biology, Vol. 5, Issue 2
Communication and Computation by Bacteria Compartmentalized within Microemulsion Droplets
journal, December 2013
- Weitz, Maximilian; Mückl, Andrea; Kapsner, Korbinian
- Journal of the American Chemical Society, Vol. 136, Issue 1
Cell-sized mechanosensitive and biosensing compartment programmed with DNA
journal, January 2017
- Majumder, Sagardip; Garamella, Jonathan; Wang, Ying-Lin
- Chemical Communications, Vol. 53, Issue 53
Rapid modulation of droplet composition with pincer microvalves
journal, January 2015
- Ochs, Christopher J.; Abate, Adam R.
- Lab on a Chip, Vol. 15, Issue 1
Electroporation of micro-droplet encapsulated HeLa cells in oil phase
journal, August 2010
- Xiao, Kang; Zhang, Mengying; Chen, Shuyu
- ELECTROPHORESIS, Vol. 31, Issue 18
Infection on a chip: a microscale platform for simple and sensitive cell-based virus assays
journal, January 2009
- Zhu, Ying; Warrick, Jay W.; Haubert, Kathryn
- Biomedical Microdevices, Vol. 11, Issue 3
Microdroplet-based cell culture models and their application
journal, November 2016
- Jang, Minjeong; Yang, Seungwon; Kim, Pilnam
- BioChip Journal, Vol. 10, Issue 4
High-resolution mapping of bifurcations in nonlinear biochemical circuits
journal, June 2016
- Genot, A. J.; Baccouche, A.; Sieskind, R.
- Nature Chemistry, Vol. 8, Issue 8
Microfluidic high-throughput culturing of single cells for selection based on extracellular metabolite production or consumption
journal, April 2014
- Wang, Benjamin L.; Ghaderi, Adel; Zhou, Hang
- Nature Biotechnology, Vol. 32, Issue 5
Emergent genetic oscillations in a synthetic microbial consortium
journal, August 2015
- Chen, Y.; Kim, J. K.; Hirning, A. J.
- Science, Vol. 349, Issue 6251
Microfluidic based single cell microinjection
journal, January 2008
- Adamo, Andrea; Jensen, Klavs F.
- Lab on a Chip, Vol. 8, Issue 8
Dielectrowetting manipulation for digital microfluidics: creating, transporting, splitting, and merging of droplets
journal, January 2017
- Geng, Hongyao; Feng, Jian; Stabryla, Lisa Marie
- Lab on a Chip, Vol. 17, Issue 6
Biochemical analysis on microfluidic chips
journal, June 2016
- Wu, Jing; He, Ziyi; Chen, Qiushui
- TrAC Trends in Analytical Chemistry, Vol. 80
Microfluidic Preparation of Polymer-Nucleic Acid Nanocomplexes Improves Nonviral Gene Transfer
journal, November 2013
- Grigsby, Christopher L.; Ho, Yi-Ping; Lin, Chao
- Scientific Reports, Vol. 3, Issue 1
Electrode-free picoinjection of microfluidic drops
journal, January 2012
- O'Donovan, Brian; Eastburn, Dennis J.; Abate, Adam R.
- Lab on a Chip, Vol. 12, Issue 20
Microfluidic Production of Multiple Emulsions
journal, March 2017
- Vladisavljević, Goran; Al Nuumani, Ruqaya; Nabavi, Seyed
- Micromachines, Vol. 8, Issue 3
Cell-free compartmentalized protein synthesis inside double emulsion templated liposomes with in vitro synthesized and assembled ribosomes
journal, January 2016
- Caschera, Filippo; Lee, Jin Woo; Ho, Kenneth K. Y.
- Chemical Communications, Vol. 52, Issue 31
Unilamellar vesicle formation and encapsulation by microfluidic jetting
journal, March 2008
- Stachowiak, J. C.; Richmond, D. L.; Li, T. H.
- Proceedings of the National Academy of Sciences, Vol. 105, Issue 12
Design and construction of small perturbation mutagenesis libraries for antibody affinity maturation using massive microchip-synthesized oligonucleotides
journal, January 2015
- Xu, Man; Hu, Siyi; Ding, Bo
- Journal of Biotechnology, Vol. 194
A microfluidic bioreactor for increased active retrovirus output
journal, January 2008
- Vu, Halong N.; Li, Yawen; Casali, Monica
- Lab Chip, Vol. 8, Issue 1
Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
journal, January 1981
- Beaucage, S. L.; Caruthers, M. H.
- Tetrahedron Letters, Vol. 22, Issue 20, p. 1859-1862
DNA-library assembly programmed by on-demand nano-liter droplets from a custom microfluidic chip
journal, July 2015
- Tangen, Uwe; Minero, Gabriel Antonio S.; Sharma, Abhishek
- Biomicrofluidics, Vol. 9, Issue 4
On-Chip Spyhole Mass Spectrometry for Droplet-Based Microfluidics
journal, March 2014
- Gasilova, Natalia; Yu, Qiuliyang; Qiao, Liang
- Angewandte Chemie International Edition, Vol. 53, Issue 17
Electroporation of Cells in Microfluidic Droplets
journal, March 2009
- Zhan, Yihong; Wang, Jun; Bao, Ning
- Analytical Chemistry, Vol. 81, Issue 5
Quorum-Sensing Crosstalk-Driven Synthetic Circuits: From Unimodality to Trimodality
journal, December 2014
- Wu, Fuqing; Menn, David J.; Wang, Xiao
- Chemistry & Biology, Vol. 21, Issue 12
A Droplet Microfluidic Platform for Automating Genetic Engineering
journal, February 2016
- Gach, Philip C.; Shih, Steve C. C.; Sustarich, Jess
- ACS Synthetic Biology, Vol. 5, Issue 5
Selective Encapsulation of Single Cells and Subcellular Organelles into Picoliter- and Femtoliter-Volume Droplets
journal, March 2005
- He, Mingyan; Edgar, J. Scott; Jeffries, Gavin D. M.
- Analytical Chemistry, Vol. 77, Issue 6
Automated electrotransformation of Escherichia coli on a digital microfluidic platform using bioactivated magnetic beads
journal, January 2017
- Moore, J. A.; Nemat-Gorgani, M.; Madison, A. C.
- Biomicrofluidics, Vol. 11, Issue 1
Fluorescence-activated droplet sorting (FADS): efficient microfluidic cell sorting based on enzymatic activity
journal, January 2009
- Baret, Jean-Christophe; Miller, Oliver J.; Taly, Valerie
- Lab on a Chip, Vol. 9, Issue 13, p. 1850-1858
CRISPR-Cas9 delivery to hard-to-transfect cells via membrane deformation
journal, August 2015
- Han, Xin; Liu, Zongbin; Jo, Myeong chan
- Science Advances, Vol. 1, Issue 7
Synthetic biology: applications come of age
journal, May 2010
- Khalil, Ahmad S.; Collins, James J.
- Nature Reviews Genetics, Vol. 11, Issue 5
A general strategy for expanding polymerase function by droplet microfluidics
journal, April 2016
- Larsen, Andrew C.; Dunn, Matthew R.; Hatch, Andrew
- Nature Communications, Vol. 7, Issue 1
Comparison of plasma sample purification by manual liquid–liquid extraction, automated 96-well liquid–liquid extraction and automated 96-well solid-phase extraction for analysis by high-performance liquid chromatography with tandem mass spectrometry
journal, September 1999
- Jemal, Mohammed; Teitz, Deborah; Ouyang, Zheng
- Journal of Chromatography B: Biomedical Sciences and Applications, Vol. 732, Issue 2
Diversity in the dynamical behaviour of a compartmentalized programmable biochemical oscillator
journal, February 2014
- Weitz, Maximilian; Kim, Jongmin; Kapsner, Korbinian
- Nature Chemistry, Vol. 6, Issue 4
Microfluidics-to-mass spectrometry: A review of coupling methods and applications
journal, February 2015
- Wang, Xue; Yi, Lian; Mukhitov, Nikita
- Journal of Chromatography A, Vol. 1382
Surfactants in droplet-based microfluidics
journal, January 2012
- Baret, Jean-Christophe
- Lab Chip, Vol. 12, Issue 3
Microfluidic screening and whole-genome sequencing identifies mutations associated with improved protein secretion by yeast
journal, August 2015
- Huang, Mingtao; Bai, Yunpeng; Sjostrom, Staffan L.
- Proceedings of the National Academy of Sciences, Vol. 112, Issue 34
Integrated microbioreactor for culture and analysis of bacteria, algae and yeast
journal, September 2010
- Au, Sam H.; Shih, Steve C. C.; Wheeler, Aaron R.
- Biomedical Microdevices, Vol. 13, Issue 1
Construction of membrane-bound artificial cells using microfluidics: a new frontier in bottom-up synthetic biology
journal, June 2016
- Elani, Yuval
- Biochemical Society Transactions, Vol. 44, Issue 3
Parallel gene synthesis in a microfluidic device
journal, March 2007
- Kong, D. S.; Carr, P. A.; Chen, L.
- Nucleic Acids Research, Vol. 35, Issue 8
Microfluidic Fabrication of Pluronic Vesicles with Controlled Permeability
journal, May 2016
- do Nascimento, Débora F.; Arriaga, Laura R.; Eggersdorfer, Max
- Langmuir, Vol. 32, Issue 21
Droplet-interface-bilayer assays in microfluidic passive networks
journal, April 2015
- Schlicht, Bárbara; Zagnoni, Michele
- Scientific Reports, Vol. 5, Issue 1
Vesicle-based artificial cells as chemical microreactors with spatially segregated reaction pathways
journal, October 2014
- Elani, Yuval; Law, Robert V.; Ces, Oscar
- Nature Communications, Vol. 5, Issue 1
Synthesis of high-quality libraries of long (150mer) oligonucleotides by a novel depurination controlled process
journal, March 2010
- LeProust, Emily M.; Peck, Bill J.; Spirin, Konstantin
- Nucleic Acids Research, Vol. 38, Issue 8
Highly Parallel Genome-wide Expression Profiling of Individual Cells Using Nanoliter Droplets
journal, May 2015
- Macosko, Evan Z.; Basu, Anindita; Satija, Rahul
- Cell, Vol. 161, Issue 5
j5 DNA Assembly Design Automation Software
journal, December 2011
- Hillson, Nathan J.; Rosengarten, Rafael D.; Keasling, Jay D.
- ACS Synthetic Biology, Vol. 1, Issue 1, p. 14-21
Chemical Transfection of Cells in Picoliter Aqueous Droplets in Fluorocarbon Oil
journal, November 2011
- Chen, Fangyuan; Zhan, Yihong; Geng, Tao
- Analytical Chemistry, Vol. 83, Issue 22
Principles of cell-free genetic circuit assembly
journal, October 2003
- Noireaux, V.; Bar-Ziv, R.; Libchaber, A.
- Proceedings of the National Academy of Sciences, Vol. 100, Issue 22
Protein synthesis in artificial cells: using compartmentalisation for spatial organisation in vesicle bioreactors
journal, January 2015
- Elani, Yuval; Law, Robert V.; Ces, Oscar
- Physical Chemistry Chemical Physics, Vol. 17, Issue 24
Automated Acoustic Matrix Deposition for MALDI Sample Preparation
journal, February 2006
- Aerni, Hans-Rudolf; Cornett, Dale S.; Caprioli, Richard M.
- Analytical Chemistry, Vol. 78, Issue 3
Engineered temperature compensation in a synthetic genetic clock
journal, January 2014
- Hussain, F.; Gupta, C.; Hirning, A. J.
- Proceedings of the National Academy of Sciences, Vol. 111, Issue 3
Microfluidic electro-sonoporation: a multi-modal cell poration methodology through simultaneous application of electric field and ultrasonic wave
journal, January 2013
- Longsine-Parker, Whitney; Wang, Han; Koo, Chiwan
- Lab on a Chip, Vol. 13, Issue 11
Droplet Barcoding for Single-Cell Transcriptomics Applied to Embryonic Stem Cells
journal, May 2015
- Klein, Allon M.; Mazutis, Linas; Akartuna, Ilke
- Cell, Vol. 161, Issue 5
Digital microfluidics: a versatile tool for applications in chemistry, biology and medicine
journal, January 2012
- Jebrail, Mais J.; Bartsch, Michael S.; Patel, Kamlesh D.
- Lab on a Chip, Vol. 12, Issue 14
Chemical communication between bacteria and cell-free gene expression systems within linear chains of emulsion droplets
journal, January 2016
- Schwarz-Schilling, M.; Aufinger, L.; Mückl, A.
- Integrative Biology, Vol. 8, Issue 4
Digital Microfluidic Cell Culture
journal, December 2015
- Ng, Alphonsus H. C.; Li, Bingyu Betty; Chamberlain, M. Dean
- Annual Review of Biomedical Engineering, Vol. 17, Issue 1
Lipid Bilayers on a Picoliter Microdroplet Array for Rapid Fluorescence Detection of Membrane Transport
journal, March 2014
- Tonooka, Taishi; Sato, Koji; Osaki, Toshihisa
- Small, Vol. 10, Issue 16
A vesicle bioreactor as a step toward an artificial cell assembly
journal, December 2004
- Noireaux, V.; Libchaber, A.
- Proceedings of the National Academy of Sciences, Vol. 101, Issue 51
Ultrahigh-throughput screening in drop-based microfluidics for directed evolution
journal, February 2010
- Agresti, J. J.; Antipov, E.; Abate, A. R.
- Proceedings of the National Academy of Sciences, Vol. 107, Issue 9, p. 4004-4009
Recent advances in microchip-mass spectrometry for biological analysis
journal, January 2014
- He, Xiangwei; Chen, Qiushui; Zhang, Yandong
- TrAC Trends in Analytical Chemistry, Vol. 53
Digital-to-biological converter for on-demand production of biologics
journal, May 2017
- Boles, Kent S.; Kannan, Krishna; Gill, John
- Nature Biotechnology, Vol. 35, Issue 7
Droplet microfluidics
journal, January 2008
- Teh, Shia-Yen; Lin, Robert; Hung, Lung-Hsin
- Lab on a Chip, Vol. 8, Issue 2, p. 198-220
Scalable gene synthesis by selective amplification of DNA pools from high-fidelity microchips
journal, November 2010
- Kosuri, Sriram; Eroshenko, Nikolai; LeProust, Emily M.
- Nature Biotechnology, Vol. 28, Issue 12, p. 1295-1299
High-throughput in situ cell electroporation microsystem for parallel delivery of single guide RNAs into mammalian cells
journal, February 2017
- Bian, Shengtai; Zhou, Yicen; Hu, Yawei
- Scientific Reports, Vol. 7, Issue 1
Label-free isolation and deposition of single bacterial cells from heterogeneous samples for clonal culturing
journal, September 2016
- Riba, J.; Gleichmann, T.; Zimmermann, S.
- Scientific Reports, Vol. 6, Issue 1
A synchronized quorum of genetic clocks
journal, January 2010
- Danino, Tal; Mondragón-Palomino, Octavio; Tsimring, Lev
- Nature, Vol. 463, Issue 7279
Sequence specific sorting of DNA molecules with FACS using 3dPCR
journal, January 2017
- Sukovich, David J.; Lance, Shea T.; Abate, Adam R.
- Scientific Reports, Vol. 7, Issue 1
On-chip integration of droplet microfluidics and nanostructure-initiator mass spectrometry for enzyme screening
journal, January 2017
- Heinemann, Joshua; Deng, Kai; Shih, Steve C. C.
- Lab on a Chip, Vol. 17, Issue 2
Integration of microfluidics into the synthetic biology design flow
journal, January 2014
- Huang, Haiyao; Densmore, Douglas
- Lab Chip, Vol. 14, Issue 18
Partitioning Variability of a Compartmentalized In Vitro Transcriptional Thresholding Circuit
journal, May 2015
- Kapsner, Korbinian; Simmel, Friedrich C.
- ACS Synthetic Biology, Vol. 4, Issue 10
Resource Sharing Controls Gene Expression Bursting
journal, September 2016
- Caveney, Patrick M.; Norred, S. Elizabeth; Chin, Charles W.
- ACS Synthetic Biology, Vol. 6, Issue 2
A droplet microfluidic system for sequential generation of lipid bilayers and transmembrane electrical recordings
journal, January 2015
- Czekalska, Magdalena A.; Kaminski, Tomasz S.; Jakiela, Slawomir
- Lab on a Chip, Vol. 15, Issue 2
The All E. coli TX-TL Toolbox 2.0: A Platform for Cell-Free Synthetic Biology
journal, February 2016
- Garamella, Jonathan; Marshall, Ryan; Rustad, Mark
- ACS Synthetic Biology, Vol. 5, Issue 4
Artificial cell mimics as simplified models for the study of cell biology
journal, June 2017
- Salehi-Reyhani, Ali; Ces, Oscar; Elani, Yuval
- Experimental Biology and Medicine, Vol. 242, Issue 13
Dissecting enzyme function with microfluidic-based deep mutational scanning
journal, May 2015
- Romero, Philip A.; Tran, Tuan M.; Abate, Adam R.
- Proceedings of the National Academy of Sciences, Vol. 112, Issue 23
Accurate multiplex gene synthesis from programmable DNA microchips
journal, December 2004
- Tian, Jingdong; Gong, Hui; Sheng, Nijing
- Nature, Vol. 432, Issue 7020, p. 1050-1054
Vesicles-on-a-chip: A universal microfluidic platform for the assembly of liposomes and polymersomes
journal, June 2016
- Petit, Julien; Polenz, Ingmar; Baret, Jean-Christophe
- The European Physical Journal E, Vol. 39, Issue 6
Protein aggregation with poly(vinyl) alcohol surfactant reduces double emulsion-encapsulated mammalian cell-free expression
journal, March 2017
- Ho, Kenneth K. Y.; Lee, Jin Woo; Durand, Grégory
- PLOS ONE, Vol. 12, Issue 3
Microfluidic Formation of Membrane-Free Aqueous Coacervate Droplets in Water
journal, May 2015
- van Swaay, Dirk; Tang, T. -Y. Dora; Mann, Stephen
- Angewandte Chemie International Edition, Vol. 54, Issue 29
High-throughput injection with microfluidics using picoinjectors
journal, October 2010
- Abate, A. R.; Hung, T.; Mary, P.
- Proceedings of the National Academy of Sciences, Vol. 107, Issue 45
Droplet microfluidics for high-throughput biological assays
journal, January 2012
- Guo, Mira T.; Rotem, Assaf; Heyman, John A.
- Lab on a Chip, Vol. 12, Issue 12
Trapping and release of giant unilamellar vesicles in microfluidic wells
journal, January 2014
- Yamada, Ayako; Lee, Sungyon; Bassereau, Patricia
- Soft Matter, Vol. 10, Issue 32
Single-cell genome sequencing at ultra-high-throughput with microfluidic droplet barcoding
journal, May 2017
- Lan, Freeman; Demaree, Benjamin; Ahmed, Noorsher
- Nature Biotechnology, Vol. 35, Issue 7
Rapid and tunable post-translational coupling of genetic circuits
journal, April 2014
- Prindle, Arthur; Selimkhanov, Jangir; Li, Howard
- Nature, Vol. 508, Issue 7496
Transfection of Jurkat T cells by droplet electroporation
journal, June 2017
- Im, Do Jin; Jeong, Su-Nam
- Biochemical Engineering Journal, Vol. 122
Microfluidic approaches for gene delivery and gene therapy
journal, January 2011
- Kim, Jungkyu; Hwang, Inseong; Britain, Derek
- Lab on a Chip, Vol. 11, Issue 23
Synchronized cycles of bacterial lysis for in vivo delivery
journal, July 2016
- Din, M. Omar; Danino, Tal; Prindle, Arthur
- Nature, Vol. 536, Issue 7614
Detection of Infertility-related Neutralizing Antibodies with a Cell-free Microfluidic Method
journal, November 2015
- Eyer, Klaus; Root, Katharina; Verboket, Pascal E.
- Scientific Reports, Vol. 5, Issue 1
A Versatile Microfluidic Device for Automating Synthetic Biology
journal, June 2015
- Shih, Steve C. C.; Goyal, Garima; Kim, Peter W.
- ACS Synthetic Biology, Vol. 4, Issue 10
Specific and Reversible DNA-Directed Self-Assembly of Modular Vesicle-Droplet Hybrid Materials
journal, March 2016
- Hadorn, Maik; Boenzli, Eva; Hanczyc, Martin M.
- Langmuir, Vol. 32, Issue 15
Integrated Microfluidic System for Size-Based Selection and Trapping of Giant Vesicles
journal, December 2015
- Kazayama, Yuki; Teshima, Tetsuhiko; Osaki, Toshihisa
- Analytical Chemistry, Vol. 88, Issue 2
Fluid Transport in Partially Shielded Electrowetting on Dielectric Digital Microfluidic Devices
journal, August 2016
- Madison, Andrew C.; Royal, Matthew W.; Fair, Richard B.
- Journal of Microelectromechanical Systems, Vol. 25, Issue 4
A One Pot, One Step, Precision Cloning Method with High Throughput Capability
journal, November 2008
- Engler, Carola; Kandzia, Romy; Marillonnet, Sylvestre
- PLoS ONE, Vol. 3, Issue 11, Article No. e3647
High-throughput and combinatorial gene expression on a chip for metabolism-induced toxicology screening
journal, May 2014
- Kwon, Seok Joon; Lee, Dong Woo; Shah, Dhiral A.
- Nature Communications, Vol. 5, Issue 1
On-Chip Spyhole Mass Spectrometry for Droplet-Based Microfluidics
journal, March 2014
- Gasilova, Natalia; Yu, Qiuliyang; Qiao, Liang
- Angewandte Chemie, Vol. 126, Issue 17
Synchronous long-term oscillations in a synthetic gene circuit
text, January 2016
- Potvin-Trottier, Laurent; Lord, Nathan D.; Vinnicombe, Glenn
- Apollo - University of Cambridge Repository
Detection of Infertility-related Neutralizing Antibodies with a Cell-free Microfluidic Method
text, January 2015
- Eyer, Klaus; Root, Katharina; Verboket, Pascal E.
- ETH Zurich
Works referencing / citing this record:
Cytoskeletal and Actin‐Based Polymerization Motors and Their Role in Minimal Cell Design
journal, April 2019
- Hürtgen, Daniel; Vogel, Sven Kenjiro; Schwille, Petra
- Advanced Biosystems, Vol. 3, Issue 6
Programmable Functionalization of Surfactant‐Stabilized Microfluidic Droplets via DNA‐Tags
journal, April 2019
- Jahnke, Kevin; Weiss, Marian; Frey, Christoph
- Advanced Functional Materials, Vol. 29, Issue 23
Impact of electrode design and voltage waveform on low-potential magnetohydrodynamic fluid actuation
journal, June 2019
- Warner, Cael; McDermid, Cameron M.; Ahmadi, Ali
- Microfluidics and Nanofluidics, Vol. 23, Issue 7
A flexible precise volume sensor based on metal-on-polyimide electrodes sandwiched by PDMS channel for microfluidic systems
journal, November 2019
- Pu, Zhihua; Ma, Jiaming; Li, Wenwen
- Microfluidics and Nanofluidics, Vol. 23, Issue 12
Three-dimensional numerical simulation of droplet formation in a microfluidic flow-focusing device
journal, May 2019
- Han, Wenbo; Chen, Xueye; Wu, Zhongli
- Journal of the Brazilian Society of Mechanical Sciences and Engineering, Vol. 41, Issue 6
High throughput screening of complex biological samples with mass spectrometry – from bulk measurements to single cell analysis
journal, January 2019
- Kempa, Emily E.; Hollywood, Katherine A.; Smith, Clive A.
- The Analyst, Vol. 144, Issue 3
Fluorescence lifetime-activated droplet sorting in microfluidic chip systems
journal, January 2019
- Hasan, Sadat; Geissler, David; Wink, Konstantin
- Lab on a Chip, Vol. 19, Issue 3
Liquid metal electrode-enabled flexible microdroplet sensor
journal, January 2020
- Zhang, Renchang; Ye, Zi; Gao, Meng
- Lab on a Chip, Vol. 20, Issue 3
New insights into generation of highly controllable monodisperse high-throughput microdroplets in a T-junction microchannel with step structure
journal, October 2019
- Han, Wenbo; Chen, Xueye
- Journal of Dispersion Science and Technology
Encapsulation of complex solutions using droplet microfluidics towards the synthesis of artificial cells
journal, June 2019
- Majumder, Sagardip; Wubshet, Nadab; Liu, Allen P.
- Journal of Micromechanics and Microengineering, Vol. 29, Issue 8
Mass Transfer via Femtoliter Droplets in Ping-Pong Mode
journal, January 2020
- Shojaeian, Mostafa; Hardt, Steffen
- Physical Review Applied, Vol. 13, Issue 1
High-throughput magnetic particle washing in nanoliter droplets using serial injection and splitting
journal, June 2018
- Stephenson, William
- Micro and Nano Systems Letters, Vol. 6, Issue 1
Cell-Free Approaches in Synthetic Biology Utilizing Microfluidics
journal, March 2018
- Damiati, Samar; Mhanna, Rami; Kodzius, Rimantas
- Genes, Vol. 9, Issue 3
The Usual Suspects 2019: of Chips, Droplets, Synthesis, and Artificial Cells
journal, April 2019
- Eilenberger, Christoph; Spitz, Sarah; Bachmann, Barbara Eva Maria
- Micromachines, Vol. 10, Issue 5
Nanofiber membrane supported lung-on-a-chip microdevice for anti-cancer drug testing
journal, January 2018
- Yang, Xingyuan; Li, Kaiyan; Zhang, Xu
- Lab on a Chip, Vol. 18, Issue 3
Antifouling digital microfluidics using lubricant infused porous film
journal, January 2019
- Geng, Hongyao; Cho, Sung Kwon
- Lab on a Chip, Vol. 19, Issue 13
Synthetic biology UK: progress, paradigms and prospects
journal, December 2017
- Clarke, Lionel J.
- Engineering Biology, Vol. 1, Issue 2
Role of Digital Microfluidics in Enabling Access to Laboratory Automation and Making Biology Programmable
journal, June 2020
- Kothamachu, Varun B.; Zaini, Sabrina; Muffatto, Federico
- SLAS TECHNOLOGY: Translating Life Sciences Innovation
Creation of Artificial Cell-Like Structures Promoted by Microfluidics Technologies
journal, March 2019
- Sato, Yusuke; Takinoue, Masahiro
- Micromachines, Vol. 10, Issue 4
Label-Free Sensing in Microdroplet-Based Microfluidic Systems
journal, May 2018
- Kalantarifard, Ali; Saateh, Abtin; Elbuken, Caglar
- Chemosensors, Vol. 6, Issue 2
Mastering Complexity: Towards Bottom-up Construction of Multifunctional Eukaryotic Synthetic Cells
journal, September 2018
- Göpfrich, Kerstin; Platzman, Ilia; Spatz, Joachim P.
- Trends in Biotechnology, Vol. 36, Issue 9
Simultaneous monitoring of transcription and translation in mammalian cell-free expression in bulk and in cell-sized droplets
journal, January 2018
- Wang, Shue; Majumder, Sagardip; Emery, Nicholas J.
- Synthetic Biology, Vol. 3, Issue 1
Phenotype-Independent Isolation of Interspecies Saccharomyces Hybrids by Dual-Dye Fluorescent Staining and Fluorescence-Activated Cell Sorting
journal, April 2019
- Gorter de Vries, Arthur R.; Koster, Charlotte C.; Weening, Susan M.
- Frontiers in Microbiology, Vol. 10
Cell-Free Approaches in Synthetic Biology Utilizing Microfluidics
journal, March 2018
- Damiati, Samar; Mhanna, Rami; Kodzius, Rimantas
- Genes, Vol. 9, Issue 3
Creation of Artificial Cell-Like Structures Promoted by Microfluidics Technologies
journal, March 2019
- Sato, Yusuke; Takinoue, Masahiro
- Micromachines, Vol. 10, Issue 4
Figures/Tables have been extracted from DOE-funded journal article accepted manuscripts.