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Title: Rhodosporidium toruloides: a new platform organism for conversion of lignocellulose into terpene biofuels and bioproducts

Abstract

Economical conversion of lignocellulosic biomass into biofuels and bioproducts is central to the establishment of a robust bioeconomy. This requires a conversion host that is able to both efficiently assimilate the major lignocellulose-derived carbon sources and divert their metabolites toward specific bioproducts. In this study, the carotenogenic yeast Rhodosporidium toruloides was examined for its ability to convert lignocellulose into two non-native sesquiterpenes with biofuel (bisabolene) and pharmaceutical (amorphadiene) applications. We found that R. toruloides can efficiently convert a mixture of glucose and xylose from hydrolyzed lignocellulose into these bioproducts, and unlike many conventional production hosts, its growth and productivity were enhanced in lignocellulosic hydrolysates relative to purified substrates. This organism was demonstrated to have superior growth in corn stover hydrolysates prepared by two different pretreatment methods, one using a novel biocompatible ionic liquid (IL) choline α-ketoglutarate, which produced 261 mg/L of bisabolene at bench scale, and the other using an alkaline pretreatment, which produced 680 mg/L of bisabolene in a high-gravity fe d-batch bioreactor. Interestingly, R. toruloides was also observed to assimilate p-coumaric acid liberated from acylated grass lignin in the IL hydrolysate, a finding we verified with purified substrates. R. toruloides was also able to consume several additional compoundsmore » with aromatic motifs similar to lignin monomers, suggesting that this organism may have the metabolic potential to convert depolymerized lignin streams alongside lignocellulosic sugars. This study highlights the natural compatibility of R. toruloides with bioprocess conditions relevant to lignocellulosic biorefineries and demonstrates its ability to produce non-native terpenes.« less

Authors:
 [1];  [2];  [3];  [4];  [4];  [5];  [5];  [4];  [6];  [5];  [5];  [4];  [4];  [7];  [6];  [6];  [1];  [8];  [9];  [4]
  1. Joint BioEnergy Inst. (JBEI), Emeryville, CA (United States); Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
  2. Joint BioEnergy Inst. (JBEI), Emeryville, CA (United States); Univ. of California, Berkeley, CA (United States)
  3. Energy Biosciences Institute, Berkeley, CA (United States)
  4. Joint BioEnergy Inst. (JBEI), Emeryville, CA (United States); Sandia National Lab. (SNL-CA), Livermore, CA (United States)
  5. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
  6. Joint BioEnergy Inst. (JBEI), Emeryville, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
  7. Joint BioEnergy Inst. (JBEI), Emeryville, CA (United States); Univ. of California, Berkeley, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
  8. Energy Biosciences Institute, Berkeley, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Univ. of California, Berkeley, CA (United States)
  9. Energy Biosciences Institute, Berkeley, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1421836
Alternate Identifier(s):
OSTI ID: 1431028
Grant/Contract Number:  
[AC02-05CH11231; AC05-76RL01830]
Resource Type:
Accepted Manuscript
Journal Name:
Biotechnology for Biofuels
Additional Journal Information:
[ Journal Volume: 10; Journal Issue: 1]; Journal ID: ISSN 1754-6834
Publisher:
BioMed Central
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; Rhodosporidium toruloides; Terpenes; Bisabolene; Amorphadiene; Heterologous expression; Plant biomass-derived hydrolysate; Multiple carbon source utilization

Citation Formats

Yaegashi, Junko, Kirby, James, Ito, Masakazu, Sun, Jian, Dutta, Tanmoy, Mirsiaghi, Mona, Sundstrom, Eric R., Rodriguez, Alberto, Baidoo, Edward, Tanjore, Deepti, Pray, Todd, Sale, Kenneth, Singh, Seema, Keasling, Jay D., Simmons, Blake A., Singer, Steven W., Magnuson, Jon K., Arkin, Adam P., Skerker, Jeffrey M., and Gladden, John M. Rhodosporidium toruloides: a new platform organism for conversion of lignocellulose into terpene biofuels and bioproducts. United States: N. p., 2017. Web. doi:10.1186/s13068-017-0927-5.
Yaegashi, Junko, Kirby, James, Ito, Masakazu, Sun, Jian, Dutta, Tanmoy, Mirsiaghi, Mona, Sundstrom, Eric R., Rodriguez, Alberto, Baidoo, Edward, Tanjore, Deepti, Pray, Todd, Sale, Kenneth, Singh, Seema, Keasling, Jay D., Simmons, Blake A., Singer, Steven W., Magnuson, Jon K., Arkin, Adam P., Skerker, Jeffrey M., & Gladden, John M. Rhodosporidium toruloides: a new platform organism for conversion of lignocellulose into terpene biofuels and bioproducts. United States. doi:10.1186/s13068-017-0927-5.
Yaegashi, Junko, Kirby, James, Ito, Masakazu, Sun, Jian, Dutta, Tanmoy, Mirsiaghi, Mona, Sundstrom, Eric R., Rodriguez, Alberto, Baidoo, Edward, Tanjore, Deepti, Pray, Todd, Sale, Kenneth, Singh, Seema, Keasling, Jay D., Simmons, Blake A., Singer, Steven W., Magnuson, Jon K., Arkin, Adam P., Skerker, Jeffrey M., and Gladden, John M. Mon . "Rhodosporidium toruloides: a new platform organism for conversion of lignocellulose into terpene biofuels and bioproducts". United States. doi:10.1186/s13068-017-0927-5. https://www.osti.gov/servlets/purl/1421836.
@article{osti_1421836,
title = {Rhodosporidium toruloides: a new platform organism for conversion of lignocellulose into terpene biofuels and bioproducts},
author = {Yaegashi, Junko and Kirby, James and Ito, Masakazu and Sun, Jian and Dutta, Tanmoy and Mirsiaghi, Mona and Sundstrom, Eric R. and Rodriguez, Alberto and Baidoo, Edward and Tanjore, Deepti and Pray, Todd and Sale, Kenneth and Singh, Seema and Keasling, Jay D. and Simmons, Blake A. and Singer, Steven W. and Magnuson, Jon K. and Arkin, Adam P. and Skerker, Jeffrey M. and Gladden, John M.},
abstractNote = {Economical conversion of lignocellulosic biomass into biofuels and bioproducts is central to the establishment of a robust bioeconomy. This requires a conversion host that is able to both efficiently assimilate the major lignocellulose-derived carbon sources and divert their metabolites toward specific bioproducts. In this study, the carotenogenic yeast Rhodosporidium toruloides was examined for its ability to convert lignocellulose into two non-native sesquiterpenes with biofuel (bisabolene) and pharmaceutical (amorphadiene) applications. We found that R. toruloides can efficiently convert a mixture of glucose and xylose from hydrolyzed lignocellulose into these bioproducts, and unlike many conventional production hosts, its growth and productivity were enhanced in lignocellulosic hydrolysates relative to purified substrates. This organism was demonstrated to have superior growth in corn stover hydrolysates prepared by two different pretreatment methods, one using a novel biocompatible ionic liquid (IL) choline α-ketoglutarate, which produced 261 mg/L of bisabolene at bench scale, and the other using an alkaline pretreatment, which produced 680 mg/L of bisabolene in a high-gravity fe d-batch bioreactor. Interestingly, R. toruloides was also observed to assimilate p-coumaric acid liberated from acylated grass lignin in the IL hydrolysate, a finding we verified with purified substrates. R. toruloides was also able to consume several additional compounds with aromatic motifs similar to lignin monomers, suggesting that this organism may have the metabolic potential to convert depolymerized lignin streams alongside lignocellulosic sugars. This study highlights the natural compatibility of R. toruloides with bioprocess conditions relevant to lignocellulosic biorefineries and demonstrates its ability to produce non-native terpenes.},
doi = {10.1186/s13068-017-0927-5},
journal = {Biotechnology for Biofuels},
number = [1],
volume = [10],
place = {United States},
year = {2017},
month = {10}
}

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Free Publicly Available Full Text
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Cited by: 15 works
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Figures / Tables:

Fig. 1 Fig. 1 : Schematic of R. toruloides as a new platform for the production of lignocellulosic biofuels and bioproducts

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