A Comparative Analysis of the CO‐Reducing Activities of MoFe Proteins Containing Mo‐ and V‐Nitrogenase Cofactors

Lee, Chi Chung; Tanifuji, Kazuki; Newcomb, Megan; Liedtke, Jasper; Hu, Yilin; Ribbe, Markus W.

doi:10.1002/cbic.201800035

Title: A Comparative Analysis of the CO‐Reducing Activities of MoFe Proteins Containing Mo‐ and V‐Nitrogenase Cofactors

Journal Article · 15 February 2018 · ChemBioChem: a European journal of chemical biology

DOI: https://doi.org/10.1002/cbic.201800035 · OSTI ID:1421569

Lee, Chi Chung ^[1]; Tanifuji, Kazuki ^[1]; Newcomb, Megan ^[2]; Liedtke, Jasper ^[1]; Hu, Yilin ^[1];

^[2]

Department of Molecular Biology and Biochemistry University of California 2230/2236 McGaugh Hall Irvine CA 92697-3900 USA
Department of Molecular Biology and Biochemistry University of California 2230/2236 McGaugh Hall Irvine CA 92697-3900 USA, Department of Chemistry University of California 2236 McGaugh Hall Irvine CA 92697-2025 USA

Abstract The Mo and V nitrogenases are structurally homologous yet catalytically distinct in their abilities to reduce CO to hydrocarbons. Here we report a comparative analysis of the CO‐reducing activities of the Mo‐ and V‐nitrogenase cofactors (i.e., the M and V clusters) upon insertion of the respective cofactor into the same, cofactor‐deficient MoFe protein scaffold. Our data reveal a combined contribution from the protein environment and cofactor properties to the reactivity of nitrogenase toward CO, thus laying a foundation for further mechanistic investigation of the enzymatic CO reduction, while suggesting the potential of targeting both the protein scaffold and the cofactor species for nitrogenase‐based applications in the future.

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Sponsoring Organization:: USDOE

Grant/Contract Number:: SC0014470

OSTI ID:: 1421569

Journal Information:: ChemBioChem: a European journal of chemical biology, Journal Name: ChemBioChem: a European journal of chemical biology Journal Issue: 7 Vol. 19; ISSN 1439-4227

Publisher:: Wiley Blackwell (John Wiley & Sons)Copyright Statement

Country of Publication:: France

Language:: English

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