Impaired phloem loading in zmsweet13a,b,c sucrose transporter triple knock‐out mutants in Zea mays
Abstract
Summary Crop yield depends on efficient allocation of sucrose from leaves to seeds. In Arabidopsis, phloem loading is mediated by a combination of SWEET sucrose effluxers and subsequent uptake by SUT 1/ SUC 2 sucrose/H + symporters. Zm SUT 1 is essential for carbon allocation in maize, but the relative contribution to apoplasmic phloem loading and retrieval of sucrose leaking from the translocation path is not known. Here we analysed the contribution of SWEET s to phloem loading in maize. We identified three leaf‐expressed SWEET sucrose transporters as key components of apoplasmic phloem loading in Zea mays L. Zm SWEET 13 paralogues (a, b, c) are among the most highly expressed genes in the leaf vasculature. Genome‐edited triple knock‐out mutants were severely stunted. Photosynthesis of mutants was impaired and leaves accumulated high levels of soluble sugars and starch. RNA ‐seq revealed profound transcriptional deregulation of genes associated with photosynthesis and carbohydrate metabolism. Genome‐wide association study (GWAS) analyses may indicate that variability in Zm SWEET 13s correlates with agronomical traits, especifically flowering time and leaf angle. This work provides support for cooperation of three Zm SWEET 13s with Zm SUT 1 in phloem loading in Z. mays .
- Authors:
-
- Institute for Molecular Physiology Heinrich Heine University Düsseldorf Düsseldorf 40225 Germany, Max Planck Institute for Plant Breeding Research Cologne 50829 Germany, Department of Plant Biology Carnegie Science 260 Panama St Stanford CA 94305 USA
- Department of Plant Biology Carnegie Science 260 Panama St Stanford CA 94305 USA
- Department of Genetics, Development, and Cell Biology Iowa State University Ames IA 50011 USA
- Department of Agronomy and Horticulture University of Nebraska‐Lincoln Lincoln NE 68588 USA
- Publication Date:
- Research Org.:
- Carnegie Inst. of Washington, Washington, DC (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES)
- OSTI Identifier:
- 1421567
- Alternate Identifier(s):
- OSTI ID: 1421568; OSTI ID: 1500215
- Grant/Contract Number:
- DE‐FG02‐04ER15542; FG02-04ER15542
- Resource Type:
- Published Article
- Journal Name:
- New Phytologist
- Additional Journal Information:
- Journal Name: New Phytologist Journal Volume: 218 Journal Issue: 2; Journal ID: ISSN 0028-646X
- Publisher:
- Wiley-Blackwell
- Country of Publication:
- United Kingdom
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Bezrutczyk, Margaret, Hartwig, Thomas, Horschman, Marc, Char, Si Nian, Yang, Jinliang, Yang, Bing, Frommer, Wolf B., and Sosso, Davide. Impaired phloem loading in zmsweet13a,b,c sucrose transporter triple knock‐out mutants in Zea mays. United Kingdom: N. p., 2018.
Web. doi:10.1111/nph.15021.
Bezrutczyk, Margaret, Hartwig, Thomas, Horschman, Marc, Char, Si Nian, Yang, Jinliang, Yang, Bing, Frommer, Wolf B., & Sosso, Davide. Impaired phloem loading in zmsweet13a,b,c sucrose transporter triple knock‐out mutants in Zea mays. United Kingdom. https://doi.org/10.1111/nph.15021
Bezrutczyk, Margaret, Hartwig, Thomas, Horschman, Marc, Char, Si Nian, Yang, Jinliang, Yang, Bing, Frommer, Wolf B., and Sosso, Davide. Fri .
"Impaired phloem loading in zmsweet13a,b,c sucrose transporter triple knock‐out mutants in Zea mays". United Kingdom. https://doi.org/10.1111/nph.15021.
@article{osti_1421567,
title = {Impaired phloem loading in zmsweet13a,b,c sucrose transporter triple knock‐out mutants in Zea mays},
author = {Bezrutczyk, Margaret and Hartwig, Thomas and Horschman, Marc and Char, Si Nian and Yang, Jinliang and Yang, Bing and Frommer, Wolf B. and Sosso, Davide},
abstractNote = {Summary Crop yield depends on efficient allocation of sucrose from leaves to seeds. In Arabidopsis, phloem loading is mediated by a combination of SWEET sucrose effluxers and subsequent uptake by SUT 1/ SUC 2 sucrose/H + symporters. Zm SUT 1 is essential for carbon allocation in maize, but the relative contribution to apoplasmic phloem loading and retrieval of sucrose leaking from the translocation path is not known. Here we analysed the contribution of SWEET s to phloem loading in maize. We identified three leaf‐expressed SWEET sucrose transporters as key components of apoplasmic phloem loading in Zea mays L. Zm SWEET 13 paralogues (a, b, c) are among the most highly expressed genes in the leaf vasculature. Genome‐edited triple knock‐out mutants were severely stunted. Photosynthesis of mutants was impaired and leaves accumulated high levels of soluble sugars and starch. RNA ‐seq revealed profound transcriptional deregulation of genes associated with photosynthesis and carbohydrate metabolism. Genome‐wide association study (GWAS) analyses may indicate that variability in Zm SWEET 13s correlates with agronomical traits, especifically flowering time and leaf angle. This work provides support for cooperation of three Zm SWEET 13s with Zm SUT 1 in phloem loading in Z. mays .},
doi = {10.1111/nph.15021},
journal = {New Phytologist},
number = 2,
volume = 218,
place = {United Kingdom},
year = {Fri Feb 16 00:00:00 EST 2018},
month = {Fri Feb 16 00:00:00 EST 2018}
}
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