Radiation damage in a micron-sized protein crystal studied via reciprocal space mapping and Bragg coherent diffractive imaging
Abstract
For laboratory and synchrotron based X-ray sources, radiation damage has posed a significant barrier to obtaining high-resolution structural data from biological macromolecules. The problem is particularly acute for micron-sized crystals where the weaker signal often necessitates the use of higher intensity beams to obtain the relevant data. Here, we employ a combination of techniques, including Bragg coherent diffractive imaging to characterise the radiation induced damage in a micron-sized protein crystal over time. The approach we adopt here could help screen for potential protein crystal candidates for measurement at X-ray free election laser sources.
- Authors:
-
- La Trobe Univ., Melbourne (Australia); CSIRO Manufacturing Flagship, Parkville (Australia)
- La Trobe Univ., Melbourne (Australia)
- Univ. of Oxford, Oxford (United Kingdom)
- SLAC National Accelerator Lab., Menlo Park, CA (United States); Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany)
- Argonne National Lab. (ANL), Argonne, IL (United States)
- La Trobe Univ., Melbourne (Australia); Melbourne Centre for Nanofabrication, Melbourne (Australia)
- Publication Date:
- Research Org.:
- Argonne National Laboratory (ANL), Argonne, IL (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES)
- OSTI Identifier:
- 1212299
- Alternate Identifier(s):
- OSTI ID: 1421048
- Grant/Contract Number:
- AC02-06CH11357
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Structural Dynamics
- Additional Journal Information:
- Journal Volume: 2; Journal Issue: 4; Journal ID: ISSN 2329-7778
- Publisher:
- American Crystallographic Association/AIP
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY; proteins; radiation damage; x-ray diffraction; crystal structure; reciprocal space
Citation Formats
Coughlan, H. D., Darmanin, C., Phillips, N. W., Hofmann, F., Clark, J. N., Harder, R. J., Vine, D. J., and Abbey, B. Radiation damage in a micron-sized protein crystal studied via reciprocal space mapping and Bragg coherent diffractive imaging. United States: N. p., 2015.
Web. doi:10.1063/1.4919641.
Coughlan, H. D., Darmanin, C., Phillips, N. W., Hofmann, F., Clark, J. N., Harder, R. J., Vine, D. J., & Abbey, B. Radiation damage in a micron-sized protein crystal studied via reciprocal space mapping and Bragg coherent diffractive imaging. United States. https://doi.org/10.1063/1.4919641
Coughlan, H. D., Darmanin, C., Phillips, N. W., Hofmann, F., Clark, J. N., Harder, R. J., Vine, D. J., and Abbey, B. Wed .
"Radiation damage in a micron-sized protein crystal studied via reciprocal space mapping and Bragg coherent diffractive imaging". United States. https://doi.org/10.1063/1.4919641. https://www.osti.gov/servlets/purl/1212299.
@article{osti_1212299,
title = {Radiation damage in a micron-sized protein crystal studied via reciprocal space mapping and Bragg coherent diffractive imaging},
author = {Coughlan, H. D. and Darmanin, C. and Phillips, N. W. and Hofmann, F. and Clark, J. N. and Harder, R. J. and Vine, D. J. and Abbey, B.},
abstractNote = {For laboratory and synchrotron based X-ray sources, radiation damage has posed a significant barrier to obtaining high-resolution structural data from biological macromolecules. The problem is particularly acute for micron-sized crystals where the weaker signal often necessitates the use of higher intensity beams to obtain the relevant data. Here, we employ a combination of techniques, including Bragg coherent diffractive imaging to characterise the radiation induced damage in a micron-sized protein crystal over time. The approach we adopt here could help screen for potential protein crystal candidates for measurement at X-ray free election laser sources.},
doi = {10.1063/1.4919641},
journal = {Structural Dynamics},
number = 4,
volume = 2,
place = {United States},
year = {Wed Apr 29 00:00:00 EDT 2015},
month = {Wed Apr 29 00:00:00 EDT 2015}
}
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Works referencing / citing this record:
Resolution and dose dependence of radiation damage in biomolecular systems
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Bragg coherent diffraction imaging and metrics for radiation damage in protein micro-crystallography
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