DOE PAGES title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Flow-aligned, single-shot fiber diffraction using a femtosecond X-ray free-electron laser

Journal Article · · Cytoskeleton
DOI: https://doi.org/10.1002/cm.21378 · OSTI ID:1420139
 [1];  [2];  [3];  [1];  [2];  [4];  [1];  [5];  [6];  [6];  [6];  [7];  [8];  [8];  [8];  [9];  [6];  [10];  [1];  [6] more »;  [6];  [8];  [11];  [9];  [12];  [13];  [12];  [14];  [6];  [6];  [6];  [9];  [5];  [5];  [5];  [15];  [10];  [11];  [5];  [6];  [6];  [7];  [16];  [6];  [8];  [17] « less
  1. A*STAR (Agency for Science, Technology and Research) (Singapore)
  2. National Univ. of Singapore (Singapore)
  3. A*STAR (Agency for Science, Technology and Research) (Singapore); National Univ. of Singapore (Singapore)
  4. Univ. of Michigan, Ann Arbor, MI (United States)
  5. SLAC National Accelerator Lab., Menlo Park, CA (United States)
  6. Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany)
  7. Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany); Univ. of Hamburg, Hamburg (Germany)
  8. Univ. of Gothenburg, Gothenburg (Sweden)
  9. Univ. of California, Los Angeles, CA (United States)
  10. Univ. of Canterbury, Christchurch (New Zealand)
  11. Institut Laue-Langevin, Grenoble (France); Keele Univ., Staffordshire (United Kingdom)
  12. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
  13. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Howard Hughes Medical Institute, Ashburn, VA (United States)
  14. Uppsala Univ., Uppsala (Sweden)
  15. Northeastern Univ., Boston, MA (United States)
  16. National Univ. of Singapore (Singapore); A*STAR (Agency for Science, Technology and Research) (Singapore)
  17. National Univ. of Singapore (Singapore); A*STAR (Agency for Science, Technology and Research) (Singapore); Okayama Univ., Okayama (Japan)

Here, a major goal for X-ray free-electron laser (XFEL) based science is to elucidate structures of biological molecules without the need for crystals. Filament systems may provide some of the first single macromolecular structures elucidated by XFEL radiation, since they contain one-dimensional translational symmetry and thereby occupy the diffraction intensity region between the extremes of crystals and single molecules. Here, we demonstrate flow alignment of as few as 100 filaments (Escherichia coli pili, F-actin, and amyloid fibrils), which when intersected by femtosecond X-ray pulses result in diffraction patterns similar to those obtained from classical fiber diffraction studies. We also determine that F-actin can be flow-aligned to a disorientation of approximately 5 degrees. Using this XFEL-based technique, we determine that gelsolin amyloids are comprised of stacked β-strands running perpendicular to the filament axis, and that a range of order from fibrillar to crystalline is discernable for individual α-synuclein amyloids.

Research Organization:
SLAC National Accelerator Laboratory (SLAC), Menlo Park, CA (United States); Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Basic Energy Sciences (BES)
Grant/Contract Number:
AC02-76SF00515; AC02-05CH11231
OSTI ID:
1420139
Alternate ID(s):
OSTI ID: 1393240
Journal Information:
Cytoskeleton, Vol. 74, Issue 12; ISSN 1949-3584
Publisher:
WileyCopyright Statement
Country of Publication:
United States
Language:
English
Citation Metrics:
Cited by: 9 works
Citation information provided by
Web of Science

References (79)

Cheetah : software for high-throughput reduction and analysis of serial femtosecond X-ray diffraction data journal May 2014
The Coherent X-ray Imaging (CXI) instrument at the Linac Coherent Light Source (LCLS) journal March 2010
Single-particle coherent diffractive imaging with a soft x-ray free electron laser: towards soot aerosol morphology journal September 2010
Atomic model of the actin filament journal September 1990
Microtubules in Bacteria: Ancient Tubulins Build a Five-Protofilament Homolog of the Eukaryotic Cytoskeleton journal December 2011
THE CLASSIC: Molecular Structure of Nucleic Acids: A Structure for Deoxyribose Nucleic Acid journal January 2007
Chaperone-Usher Fimbriae of Escherichia coli journal January 2013
High-Resolution Protein Structure Determination by Serial Femtosecond Crystallography journal May 2012
A review of actin binding proteins: new perspectives journal October 2007
Molecular Structure Of Nucleic Acids: A Structure for Deoxyribose Nucleic Acid journal April 2003
Methods of preparing well-orientated sols of f-actin containing filaments suitable for X-ray diffraction journal October 1987
Molecular Structure of Nucleic Acids: A Structure for Deoxyribose Nucleic Acid journal April 1974
Das R�ntgen-Faserdiagramm journal December 1922
A reconstruction algorithm for single-particle diffraction imaging experiments journal August 2009
Structural polymorphism of bacterial adhesion pili journal January 1995
Amyloid fibrils: Abnormal protein assembly journal July 2008
Molecular Structure of Nucleic Acids: Molecular Structure of Deoxypentose Nucleic Acids journal April 1953
Structure of tobacco mosaic virus at 3.6 A resolution: implications for assembly journal March 1986
Helical structure of P pili from Escherichia coli journal December 1992
Accurate macromolecular structures using minimal measurements from X-ray free-electron lasers journal March 2014
Gelsolin: The tail of a molecular gymnast: Gelsolin Superfamily Proteins journal June 2013
The Effects of Shape on the Interaction of Colloidal Particles journal May 1949
Amyloid fibrils: Abnormal protein assembly journal July 2008
A Peptide Motif Consisting of Glycine, Alanine, and Valine Is Required for the Fibrillization and Cytotoxicity of Human α-Synuclein journal July 2003
FGL chaperone-assembled fimbrial polyadhesins: anti-immune armament of Gram-negative bacterial pathogens journal July 2007
X-Ray and Crystallographic Studies of Plant Virus Preparations. iii journal September 1941
Reconstruction algorithm for single-particle diffraction imaging experiments journal August 2009
X-ray lasers for structural and dynamic biology journal September 2012
Chaperone-Usher Fimbriae of Escherichia coli journal January 2013
Molecular basis of two subfamilies of immunoglobulin-like chaperones. journal August 1996
Natural and synthetic prion structure from X-ray fiber diffraction journal September 2009
Effect of the Length and Effective Diameter of F-Actin on the Filament Orientation in Liquid Crystalline Sols Measured by X-Ray Fiber Diffraction journal December 1998
X-Ray and Crystallographic Studies of Plant Virus Preparations: i. Introduction and Preparation of Specimens ii. Modes of Aggregation of the Virus Particles journal September 1941
Extensional stresses during polymer flow in ducts journal March 1978
Gas dynamic virtual nozzle for generation of microscopic droplet streams journal September 2008
The nature of the globular- to fibrous-actin transition journal January 2009
Donor strand exchange and conformational changes during E. coli fimbrial formation journal December 2010
Atomic model of the actin filament journal September 1990
Methods of preparing well-orientated sols of f-actin containing filaments suitable for X-ray diffraction journal October 1987
Fractal morphology, imaging and mass spectrometry of single aerosol particles in flight journal June 2012
“Cross-β” conformation in proteins journal March 1968
Molecular Structure of Nucleic Acids: Molecular Structure of Deoxypentose Nucleic Acids journal April 1953
Identification of genes subject to positive selection in uropathogenic strains of Escherichia coli: A comparative genomics approach journal April 2006
A Hydrophobic Stretch of 12 Amino Acid Residues in the Middle of α-Synuclein Is Essential for Filament Assembly journal November 2000
Polymer crystallography book January 2010
Structural polymorphism of bacterial adhesion pili journal January 1995
Peptides corresponding to gelsolin derived amyloid of the Finnish type (AGel FIN ) adopt two distinct forms in solution of which only one can polymerize into amyloid fibrils and form complexes with apoE : Original Articles journal January 2002
Gas Dynamic Virtual Nozzle for Generation of Microscopic Droplet Streams text January 2008
Review: Formation and Properties of Amyloid-like Fibrils Derived from α-Synuclein and Related Proteins journal June 2000
Many ways to build an actin filament: Actin filament systems journal March 2011
Diffraction by helical structures journal March 1958
The Interplay between Viscoelastic and Thermodynamic Properties Determines the Birefringence of F-Actin Gels journal July 2005
Potential for biomolecular imaging with femtosecond X-ray pulses journal August 2000
Multiple-step method for making exceptionally well-oriented liquid-crystalline sols of macromolecular assemblies journal May 1998
X-ray lasers for structural and dynamic biology journal September 2012
Das R�ntgen-Faserdiagramm journal December 1921
Formation of liquid crystalline phase of actin filament solutions and its dependence on filament length as studied by optical birefringence journal January 1991
Antigenic quantitation of type 1 fimbriae on the surface of Escherichia coli cells by an enzyme-linked immunosorbent inhibition assay. journal January 1982
Molecular configuration in sodium thymonucleate journal March 2004
First lasing and operation of an ångstrom-wavelength free-electron laser journal August 2010
Molecular Configuration in Sodium Thymonucleate journal April 1953
Structure and switching of bacterial flagellar filaments studied by X-ray fiber diffraction journal February 1998
Crystallography and Electron Microscopy of Chaperone/Usher Pilus Systems book January 2011
Structure and switching of bacterial flagellar filaments studied by X-ray fiber diffraction journal February 1998
The functional domain grouping of microtubule associated proteins journal July 2008
The structure of native G-actin journal June 2010
Phylogenetic analysis identifies many uncharacterized actin-like proteins (Alps) in bacteria: regulated polymerization, dynamic instability and treadmilling in Alp7A journal August 2009
Gelsolin amyloidosis: genetics, biochemistry, pathology and possible strategies for therapeutic intervention journal January 2012
Ca2+ binding by domain 2 plays a critical role in the activation and stabilization of gelsolin journal August 2009
Molecular Structure of Nucleic Acids: A Structure for Deoxyribose Nucleic Acid journal April 1953
Novel Actin-like Filament Structure from Clostridium tetani journal April 2012
FGL chaperone-assembled fimbrial polyadhesins: anti-immune armament of Gram-negative bacterial pathogens journal July 2007
The Structure, Function, Synthesis and Genetic Control of Bacterial pili and a Molecular Model for dna and rna Transport in gram Negative Bacteria* journal June 1965
The Interplay between Viscoelastic and Thermodynamic Properties Determines the Birefringence of F-Actin Gels journal July 2005
The Effects of Shape on the Interaction of Colloidal Particles journal May 1949
Microtubules in Bacteria: Ancient Tubulins Build a Five-Protofilament Homolog of the Eukaryotic Cytoskeleton journal December 2011
Structure of tobacco mosaic virus at 3.6 A resolution: implications for assembly journal March 1986
Molecular structure of the ParM polymer and the mechanism leading to its nucleotide-driven dynamic instability journal January 2008
The Regulation of Rabbit Skeletal Muscle Contraction journal August 1971

Cited By (11)

Femtosecond X-ray coherent diffraction of aligned amyloid fibrils on low background graphene journal May 2018
My various thoughts on actin journal January 2018
Coherent diffractive imaging of microtubules using an X-ray laser journal June 2019
Ultracompact 3D microfluidics for time-resolved structural biology text January 2020
The Potential of EuPRAXIA@SPARC_LAB for Radiation Based Techniques journal March 2019
Ultracompact 3D microfluidics for time-resolved structural biology journal January 2020
Coherent diffractive imaging of microtubules using an X-ray laser text January 2019
The Potential of EuPRAXIA@SPARC_LAB for Radiation Based Techniques journal March 2019
Ultracompact 3D microfluidics for time-resolved structural biology journal January 2020
Ultracompact 3D microfluidics for time-resolved structural biology text January 2020
Femtosecond X-ray coherent diffraction of aligned amyloid fibrils on low background graphene text January 2018

Figures / Tables (5)