Dynamic intramolecular regulation of the histone chaperone nucleoplasmin controls histone binding and release
Abstract
Here, nucleoplasmin (Npm) is a highly conserved histone chaperone responsible for the maternal storage and zygotic release of histones H2A/H2B. Npm contains a pentameric N-terminal core domain and an intrinsically disordered C-terminal tail domain. Though intrinsically disordered regions are common among histone chaperones, their roles in histone binding and chaperoning remain unclear. Using an NMR-based approach, here we demonstrate that the Xenopus laevis Npm tail domain controls the binding of histones at its largest acidic stretch (A2) via direct competition with both the C-terminal basic stretch and basic nuclear localization signal. NMR and small-angle X-ray scattering (SAXS) structural analyses allowed us to construct models of both the tail domain and the pentameric complex. Functional analyses demonstrate that these competitive intramolecular interactions negatively regulate Npm histone chaperone activity in vitro. Together these data establish a potentially generalizable mechanism of histone chaperone regulation via dynamic and specific intramolecular shielding of histone interaction sites.
- Authors:
-
- Albert Einstein College of Medicine, Bronx, NY (United States)
- Stanford Univ., Menlo Park, CA (United States)
- Albert Einstein College of Medicine, Bronx, NY (United States); Rockefeller Univ., New York, NY (United States)
- Publication Date:
- Research Org.:
- SLAC National Accelerator Laboratory (SLAC), Menlo Park, CA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1420044
- Grant/Contract Number:
- AC02-76SF00515
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Nature Communications
- Additional Journal Information:
- Journal Volume: 8; Journal Issue: 1; Journal ID: ISSN 2041-1723
- Publisher:
- Nature Publishing Group
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
Citation Formats
Warren, Christopher, Matsui, Tsutomu, Karp, Jerome M., Onikubo, Takashi, Cahill, Sean, Brenowitz, Michael, Cowburn, David, Girvin, Mark, and Shechter, David. Dynamic intramolecular regulation of the histone chaperone nucleoplasmin controls histone binding and release. United States: N. p., 2017.
Web. doi:10.1038/s41467-017-02308-3.
Warren, Christopher, Matsui, Tsutomu, Karp, Jerome M., Onikubo, Takashi, Cahill, Sean, Brenowitz, Michael, Cowburn, David, Girvin, Mark, & Shechter, David. Dynamic intramolecular regulation of the histone chaperone nucleoplasmin controls histone binding and release. United States. https://doi.org/10.1038/s41467-017-02308-3
Warren, Christopher, Matsui, Tsutomu, Karp, Jerome M., Onikubo, Takashi, Cahill, Sean, Brenowitz, Michael, Cowburn, David, Girvin, Mark, and Shechter, David. Wed .
"Dynamic intramolecular regulation of the histone chaperone nucleoplasmin controls histone binding and release". United States. https://doi.org/10.1038/s41467-017-02308-3. https://www.osti.gov/servlets/purl/1420044.
@article{osti_1420044,
title = {Dynamic intramolecular regulation of the histone chaperone nucleoplasmin controls histone binding and release},
author = {Warren, Christopher and Matsui, Tsutomu and Karp, Jerome M. and Onikubo, Takashi and Cahill, Sean and Brenowitz, Michael and Cowburn, David and Girvin, Mark and Shechter, David},
abstractNote = {Here, nucleoplasmin (Npm) is a highly conserved histone chaperone responsible for the maternal storage and zygotic release of histones H2A/H2B. Npm contains a pentameric N-terminal core domain and an intrinsically disordered C-terminal tail domain. Though intrinsically disordered regions are common among histone chaperones, their roles in histone binding and chaperoning remain unclear. Using an NMR-based approach, here we demonstrate that the Xenopus laevis Npm tail domain controls the binding of histones at its largest acidic stretch (A2) via direct competition with both the C-terminal basic stretch and basic nuclear localization signal. NMR and small-angle X-ray scattering (SAXS) structural analyses allowed us to construct models of both the tail domain and the pentameric complex. Functional analyses demonstrate that these competitive intramolecular interactions negatively regulate Npm histone chaperone activity in vitro. Together these data establish a potentially generalizable mechanism of histone chaperone regulation via dynamic and specific intramolecular shielding of histone interaction sites.},
doi = {10.1038/s41467-017-02308-3},
journal = {Nature Communications},
number = 1,
volume = 8,
place = {United States},
year = {Wed Dec 20 00:00:00 EST 2017},
month = {Wed Dec 20 00:00:00 EST 2017}
}
Web of Science
Works referenced in this record:
The Crystal Structure of Nucleoplasmin-Core
journal, October 2001
- Dutta, Shuchismita; Akey, Ildikó V.; Dingwall, Colin
- Molecular Cell, Vol. 8, Issue 4
Roles of NPM2 in Chromatin and Nucleolar Organization in Oocytes and Embryos
journal, April 2003
- Burns, K. H.
- Science, Vol. 300, Issue 5619
Structural insight into how the human helicase subunit MCM2 may act as a histone chaperone together with ASF1 at the replication fork
journal, January 2015
- Richet, Nicolas; Liu, Danni; Legrand, Pierre
- Nucleic Acids Research, Vol. 43, Issue 3
Histone chaperones in nucleosome eviction and histone exchange
journal, June 2008
- Park, Young-Jun; Luger, Karolin
- Current Opinion in Structural Biology, Vol. 18, Issue 3
Developmentally Regulated Post-translational Modification of Nucleoplasmin Controls Histone Sequestration and Deposition
journal, March 2015
- Onikubo, Takashi; Nicklay, Joshua J.; Xing, Li
- Cell Reports, Vol. 10, Issue 10
Reconstitution of mitotic chromatids with a minimum set of purified factors
journal, June 2015
- Shintomi, Keishi; Takahashi, Tatsuro S.; Hirano, Tatsuya
- Nature Cell Biology, Vol. 17, Issue 8
DAMMIF, a program for rapid ab-initio shape determination in small-angle scattering
journal, January 2009
- Franke, Daniel; Svergun, Dmitri I.
- Journal of Applied Crystallography, Vol. 42, Issue 2, p. 342-346
Two complexes that contain histones are required for nucleosome assembly in vitro: Role of nucleoplasmin and N1 in Xenopus egg extracts
journal, December 1987
- Dilworth, Stephen M.; Black, Susan J.; Laskey, Ronald A.
- Cell, Vol. 51, Issue 6
The Amber biomolecular simulation programs
journal, January 2005
- Case, David A.; Cheatham, Thomas E.; Darden, Tom
- Journal of Computational Chemistry, Vol. 26, Issue 16
Determination of the regularization parameter in indirect-transform methods using perceptual criteria
journal, August 1992
- Svergun, D. I.
- Journal of Applied Crystallography, Vol. 25, Issue 4, p. 495-503
Preferential Binding of the Histone (H3-H4) 2 Tetramer by NAP1 Is Mediated by the Amino-terminal Histone Tails
journal, August 2003
- McBryant, Steven J.; Park, Young-Jun; Abernathy, Stephanie M.
- Journal of Biological Chemistry, Vol. 278, Issue 45
Nucleosomes are assembled by an acidic protein which binds histones and transfers them to DNA
journal, October 1978
- Laskey, R. A.; Honda, B. M.; Mills, A. D.
- Nature, Vol. 275, Issue 5679
A Mechanism for Histone Chaperoning Activity of Nucleoplasmin: Thermodynamic and Structural Models
journal, October 2009
- Taneva, Stefka G.; Bañuelos, Sonia; Falces, Jorge
- Journal of Molecular Biology, Vol. 393, Issue 2
Ensemble Approach for NMR Structure Refinement against 1 H Paramagnetic Relaxation Enhancement Data Arising from a Flexible Paramagnetic Group Attached to a Macromolecule
journal, May 2004
- Iwahara, Junji; Schwieters, Charles D.; Clore, G. Marius
- Journal of the American Chemical Society, Vol. 126, Issue 18
Chromatin assembly: Cooperation between histone chaperones and ATP-dependent nucleosome remodeling machines
journal, April 2002
- Tyler, Jessica K.
- European Journal of Biochemistry, Vol. 269, Issue 9
Massive phosphorylation distinguishes Xenopus laevis nucleoplasmin isolated from oocytes or unfertilized eggs
journal, September 1986
- Cotten, Matt; Sealy, Linda; Chalkley, Roger
- Biochemistry, Vol. 25, Issue 18
New insights into nucleosome and chromatin structure: an ordered state or a disordered affair?
journal, June 2012
- Luger, Karolin; Dechassa, Mekonnen L.; Tremethick, David J.
- Nature Reviews Molecular Cell Biology, Vol. 13, Issue 7
ANP32E is a histone chaperone that removes H2A.Z from chromatin
journal, January 2014
- Obri, Arnaud; Ouararhni, Khalid; Papin, Christophe
- Nature, Vol. 505, Issue 7485
Fly Fishing for Histones: Catch and Release by Histone Chaperone Intrinsically Disordered Regions and Acidic Stretches
journal, August 2017
- Warren, Christopher; Shechter, David
- Journal of Molecular Biology, Vol. 429, Issue 16
Sensitivity of secondary structure propensities to sequence differences between α- and γ-synuclein: Implications for fibrillation
journal, December 2006
- Marsh, Joseph A.; Singh, Vinay K.; Jia, Zongchao
- Protein Science, Vol. 15, Issue 12
The Xplor-NIH NMR molecular structure determination package
journal, January 2003
- Schwieters, Charles D.; Kuszewski, John J.; Tjandra, Nico
- Journal of Magnetic Resonance, Vol. 160, Issue 1
Mutation of the small acidic tract A1 drastically reduces nucleoplasmin activity
journal, September 2004
- Salvany, Lara; Chiva, Manel; Arnan, Carme
- FEBS Letters, Vol. 576, Issue 3
Kinetic Advantage of Intrinsically Disordered Proteins in Coupled Folding–Binding Process: A Critical Assessment of the “Fly-Casting” Mechanism
journal, November 2009
- Huang, Yongqi; Liu, Zhirong
- Journal of Molecular Biology, Vol. 393, Issue 5
Two surfaces on the histone chaperone Rtt106 mediate histone binding, replication, and silencing
journal, December 2011
- Zunder, R. M.; Antczak, A. J.; Berger, J. M.
- Proceedings of the National Academy of Sciences, Vol. 109, Issue 3
The human histone chaperone sNASP interacts with linker and core histones through distinct mechanisms
journal, September 2011
- Wang, H.; Ge, Z.; Walsh, S. T. R.
- Nucleic Acids Research, Vol. 40, Issue 2
NMR Characterization of Long-Range Order in Intrinsically Disordered Proteins
journal, June 2010
- Salmon, Loïc; Nodet, Gabrielle; Ozenne, Valéry
- Journal of the American Chemical Society, Vol. 132, Issue 24
A Quantitative Characterization of Nucleoplasmin/Histone Complexes Reveals Chaperone Versatility
journal, August 2016
- Fernández-Rivero, Noelia; Franco, Aitor; Velázquez-Campoy, Adrian
- Scientific Reports, Vol. 6, Issue 1
Crystal structure of the nucleosome core particle at 2.8 Å resolution
journal, September 1997
- Luger, Karolin; Mäder, Armin W.; Richmond, Robin K.
- Nature, Vol. 389, Issue 6648
UCSF Chimera?A visualization system for exploratory research and analysis
journal, January 2004
- Pettersen, Eric F.; Goddard, Thomas D.; Huang, Conrad C.
- Journal of Computational Chemistry, Vol. 25, Issue 13
The histone shuffle: histone chaperones in an energetic dance
journal, September 2010
- Das, Chandrima; Tyler, Jessica K.; Churchill, Mair E. A.
- Trends in Biochemical Sciences, Vol. 35, Issue 9
Probabilistic Interaction Network of Evidence Algorithm and its Application to Complete Labeling of Peak Lists from Protein NMR Spectroscopy
journal, March 2009
- Bahrami, Arash; Assadi, Amir H.; Markley, John L.
- PLoS Computational Biology, Vol. 5, Issue 3
Estimation of Protein Secondary Structure from Circular Dichroism Spectra: Comparison of CONTIN, SELCON, and CDSSTR Methods with an Expanded Reference Set
journal, December 2000
- Sreerama, Narasimha; Woody, Robert W.
- Analytical Biochemistry, Vol. 287, Issue 2, p. 252-260
Structural Basis of the pH-Dependent Assembly of a Botulinum Neurotoxin Complex
journal, November 2014
- Matsui, Tsutomu; Gu, Shenyan; Lam, Kwok-ho
- Journal of Molecular Biology, Vol. 426, Issue 22
Flexible-meccano: a tool for the generation of explicit ensemble descriptions of intrinsically disordered proteins and their associated experimental observables
journal, May 2012
- Ozenne, V.; Bauer, F.; Salmon, L.
- Bioinformatics, Vol. 28, Issue 11
Quantitative Characterization of Configurational Space Sampled by HIV-1 Nucleocapsid Using Solution NMR, X-ray Scattering and Protein Engineering
journal, March 2016
- Deshmukh, Lalit; Schwieters, Charles D.; Grishaev, Alexander
- ChemPhysChem, Vol. 17, Issue 11
Activation Mechanism of the Nuclear Chaperone Nucleoplasmin: Role of the Core Domain
journal, November 2003
- Bañuelos, Sonia; Hierro, Aitor; Arizmendi, Jesús M.
- Journal of Molecular Biology, Vol. 334, Issue 3
Histone chaperones in nucleosome assembly and human disease
journal, January 2013
- Burgess, Rebecca J.; Zhang, Zhiguo
- Nature Structural & Molecular Biology, Vol. 20, Issue 1
Particle mesh Ewald: An N ⋅log( N ) method for Ewald sums in large systems
journal, June 1993
- Darden, Tom; York, Darrin; Pedersen, Lee
- The Journal of Chemical Physics, Vol. 98, Issue 12
The intrinsically disordered distal face of nucleoplasmin recognizes distinct oligomerization states of histones
journal, October 2013
- Ramos, I.; Fernandez-Rivero, N.; Arranz, R.
- Nucleic Acids Research, Vol. 42, Issue 2
Histone transfer among chaperones
journal, March 2012
- Liu, Wallace H.; Churchill, Mair E. A.
- Biochemical Society Transactions, Vol. 40, Issue 2
Intrinsically disordered regions of nucleophosmin/B23 regulate its RNA binding activity through their inter- and intra-molecular association
journal, October 2013
- Hisaoka, M.; Nagata, K.; Okuwaki, M.
- Nucleic Acids Research, Vol. 42, Issue 2
Protein fold determined by paramagnetic magic-angle spinning solid-state NMR spectroscopy
journal, March 2012
- Sengupta, Ishita; Nadaud, Philippe S.; Helmus, Jonathan J.
- Nature Chemistry, Vol. 4, Issue 5
The Catalytic Subunit of the SWR1 Remodeler Is a Histone Chaperone for the H2A.Z-H2B Dimer
journal, February 2014
- Hong, Jingjun; Feng, Hanqiao; Wang, Feng
- Molecular Cell, Vol. 53, Issue 3
New developments in the ATSAS program package for small-angle scattering data analysis
journal, March 2012
- Petoukhov, Maxim V.; Franke, Daniel; Shkumatov, Alexander V.
- Journal of Applied Crystallography, Vol. 45, Issue 2
Comparative Protein Structure Modeling Using MODELLER
journal, November 2007
- Eswar, Narayanan; Webb, Ben; Marti-Renom, Marc A.
- Current Protocols in Protein Science, Vol. 50, Issue 1
The Histone Chaperone Nap1 Promotes Nucleosome Assembly by Eliminating Nonnucleosomal Histone DNA Interactions
journal, March 2010
- Andrews, Andrew J.; Chen, Xu; Zevin, Alexander
- Molecular Cell, Vol. 37, Issue 6
Glutamylation of Nap1 modulates histone H1 dynamics and chromosome condensation in Xenopus
journal, April 2015
- Miller, Kelly E.; Heald, Rebecca
- The Journal of Cell Biology, Vol. 209, Issue 2
Prediction and Functional Analysis of Native Disorder in Proteins from the Three Kingdoms of Life
journal, March 2004
- Ward, J. J.; Sodhi, J. S.; McGuffin, L. J.
- Journal of Molecular Biology, Vol. 337, Issue 3
C-terminal acidic domain of histone chaperone human NAP1 is an efficient binding assistant for histone H2A-H2B, but not H3-H4
journal, February 2016
- Ohtomo, Hideaki; Akashi, Satoko; Moriwaki, Yoshihito
- Genes to Cells, Vol. 21, Issue 3
NMR structure of chaperone Chz1 complexed with histones H2A.Z-H2B
journal, July 2008
- Zhou, Zheng; Feng, Hanqiao; Hansen, D. Flemming
- Nature Structural & Molecular Biology, Vol. 15, Issue 8
Intrinsically disordered proteins: a 10-year recap
journal, December 2012
- Tompa, Peter
- Trends in Biochemical Sciences, Vol. 37, Issue 12
FACT Disrupts Nucleosome Structure by Binding H2A-H2B with Conserved Peptide Motifs
journal, October 2015
- Kemble, David J.; McCullough, Laura L.; Whitby, Frank G.
- Molecular Cell, Vol. 60, Issue 2
The CCPN data model for NMR spectroscopy: Development of a software pipeline
journal, April 2005
- Vranken, Wim F.; Boucher, Wayne; Stevens, Tim J.
- Proteins: Structure, Function, and Bioinformatics, Vol. 59, Issue 4
Assembly of nucleosomes: the reaction involving X. laevis nucleoplasmin
journal, September 1980
- Earnshaw, William C.; Honda, Barry M.; Laskey, Ronald A.
- Cell, Vol. 21, Issue 2
Chaperone Nap1 Shields Histone Surfaces Used in a Nucleosome and Can Put H2A-H2B in an Unconventional Tetrameric Form
journal, September 2013
- D’Arcy, Sheena; Martin, Kyle W.; Panchenko, Tanya
- Molecular Cell, Vol. 51, Issue 5
Electrostatic Interactions at the C-Terminal Domain of Nucleoplasmin Modulate Its Chromatin Decondensation Activity †
journal, May 2002
- Hierro, Aitor; Arizmendi, Jesús M.; Bañuelos, Sonia
- Biochemistry, Vol. 41, Issue 20
Water Dispersion Interactions Strongly Influence Simulated Structural Properties of Disordered Protein States
journal, April 2015
- Piana, Stefano; Donchev, Alexander G.; Robustelli, Paul
- The Journal of Physical Chemistry B, Vol. 119, Issue 16
Architectures of Whole-Module and Bimodular Proteins from the 6-Deoxyerythronolide B Synthase
journal, May 2014
- Edwards, Andrea L.; Matsui, Tsutomu; Weiss, Thomas M.
- Journal of Molecular Biology, Vol. 426, Issue 11
Improved side-chain torsion potentials for the Amber ff99SB protein force field
journal, January 2010
- Lindorff-Larsen, Kresten; Piana, Stefano; Palmo, Kim
- Proteins: Structure, Function, and Bioinformatics
Quantitative Characterization of Configurational Space Sampled by HIV-1 Nucleocapsid Using Solution NMR, X-ray Scattering and Protein Engineering
journal, March 2016
- Deshmukh, Lalit; Schwieters, Charles D.; Grishaev, Alexander
- ChemPhysChem, Vol. 17, Issue 11
The Amber biomolecular simulation programs
journal, January 2005
- Case, David A.; Cheatham, Thomas E.; Darden, Tom
- Journal of Computational Chemistry, Vol. 26, Issue 16
Cac1 WHD and PIP domains have distinct roles in replisome progression and genomic stability
journal, October 2020
- Tsirkas, Ioannis; Dovrat, Daniel; Lei, Yang
- Current Genetics, Vol. 67, Issue 1
Assembly of nucleosomes: the reaction involving X. laevis nucleoplasmin
journal, September 1980
- Earnshaw, William C.; Honda, Barry M.; Laskey, Ronald A.
- Cell, Vol. 21, Issue 2
Developmentally Regulated Post-translational Modification of Nucleoplasmin Controls Histone Sequestration and Deposition
journal, March 2015
- Onikubo, Takashi; Nicklay, Joshua J.; Xing, Li
- Cell Reports, Vol. 10, Issue 10
Mutation of the small acidic tract A1 drastically reduces nucleoplasmin activity
journal, September 2004
- Salvany, Lara; Chiva, Manel; Arnan, Carme
- FEBS Letters, Vol. 576, Issue 3
Activation Mechanism of the Nuclear Chaperone Nucleoplasmin: Role of the Core Domain
journal, November 2003
- Bañuelos, Sonia; Hierro, Aitor; Arizmendi, Jesús M.
- Journal of Molecular Biology, Vol. 334, Issue 3
Prediction and Functional Analysis of Native Disorder in Proteins from the Three Kingdoms of Life
journal, March 2004
- Ward, J. J.; Sodhi, J. S.; McGuffin, L. J.
- Journal of Molecular Biology, Vol. 337, Issue 3
A Mechanism for Histone Chaperoning Activity of Nucleoplasmin: Thermodynamic and Structural Models
journal, October 2009
- Taneva, Stefka G.; Bañuelos, Sonia; Falces, Jorge
- Journal of Molecular Biology, Vol. 393, Issue 2
Architectures of Whole-Module and Bimodular Proteins from the 6-Deoxyerythronolide B Synthase
journal, May 2014
- Edwards, Andrea L.; Matsui, Tsutomu; Weiss, Thomas M.
- Journal of Molecular Biology, Vol. 426, Issue 11
Fly Fishing for Histones: Catch and Release by Histone Chaperone Intrinsically Disordered Regions and Acidic Stretches
journal, August 2017
- Warren, Christopher; Shechter, David
- Journal of Molecular Biology, Vol. 429, Issue 16
The Histone Chaperone Nap1 Promotes Nucleosome Assembly by Eliminating Nonnucleosomal Histone DNA Interactions
journal, March 2010
- Andrews, Andrew J.; Chen, Xu; Zevin, Alexander
- Molecular Cell, Vol. 37, Issue 6
Chaperone Nap1 Shields Histone Surfaces Used in a Nucleosome and Can Put H2A-H2B in an Unconventional Tetrameric Form
journal, September 2013
- D’Arcy, Sheena; Martin, Kyle W.; Panchenko, Tanya
- Molecular Cell, Vol. 51, Issue 5
The Catalytic Subunit of the SWR1 Remodeler Is a Histone Chaperone for the H2A.Z-H2B Dimer
journal, February 2014
- Hong, Jingjun; Feng, Hanqiao; Wang, Feng
- Molecular Cell, Vol. 53, Issue 3
Histone chaperones in nucleosome eviction and histone exchange
journal, June 2008
- Park, Young-Jun; Luger, Karolin
- Current Opinion in Structural Biology, Vol. 18, Issue 3
The histone shuffle: histone chaperones in an energetic dance
journal, September 2010
- Das, Chandrima; Tyler, Jessica K.; Churchill, Mair E. A.
- Trends in Biochemical Sciences, Vol. 35, Issue 9
Intrinsically disordered proteins: a 10-year recap
journal, December 2012
- Tompa, Peter
- Trends in Biochemical Sciences, Vol. 37, Issue 12
Massive phosphorylation distinguishes Xenopus laevis nucleoplasmin isolated from oocytes or unfertilized eggs
journal, September 1986
- Cotten, Matt; Sealy, Linda; Chalkley, Roger
- Biochemistry, Vol. 25, Issue 18
Electrostatic Interactions at the C-Terminal Domain of Nucleoplasmin Modulate Its Chromatin Decondensation Activity †
journal, May 2002
- Hierro, Aitor; Arizmendi, Jesús M.; Bañuelos, Sonia
- Biochemistry, Vol. 41, Issue 20
Ensemble Approach for NMR Structure Refinement against 1 H Paramagnetic Relaxation Enhancement Data Arising from a Flexible Paramagnetic Group Attached to a Macromolecule
journal, May 2004
- Iwahara, Junji; Schwieters, Charles D.; Clore, G. Marius
- Journal of the American Chemical Society, Vol. 126, Issue 18
NMR Characterization of Long-Range Order in Intrinsically Disordered Proteins
journal, June 2010
- Salmon, Loïc; Nodet, Gabrielle; Ozenne, Valéry
- Journal of the American Chemical Society, Vol. 132, Issue 24
Water Dispersion Interactions Strongly Influence Simulated Structural Properties of Disordered Protein States
journal, April 2015
- Piana, Stefano; Donchev, Alexander G.; Robustelli, Paul
- The Journal of Physical Chemistry B, Vol. 119, Issue 16
Nucleosomes are assembled by an acidic protein which binds histones and transfers them to DNA
journal, October 1978
- Laskey, R. A.; Honda, B. M.; Mills, A. D.
- Nature, Vol. 275, Issue 5679
ANP32E is a histone chaperone that removes H2A.Z from chromatin
journal, January 2014
- Obri, Arnaud; Ouararhni, Khalid; Papin, Christophe
- Nature, Vol. 505, Issue 7485
Protein fold determined by paramagnetic magic-angle spinning solid-state NMR spectroscopy
journal, March 2012
- Sengupta, Ishita; Nadaud, Philippe S.; Helmus, Jonathan J.
- Nature Chemistry, Vol. 4, Issue 5
New insights into nucleosome and chromatin structure: an ordered state or a disordered affair?
journal, June 2012
- Luger, Karolin; Dechassa, Mekonnen L.; Tremethick, David J.
- Nature Reviews Molecular Cell Biology, Vol. 13, Issue 7
NMR structure of chaperone Chz1 complexed with histones H2A.Z-H2B
journal, July 2008
- Zhou, Zheng; Feng, Hanqiao; Hansen, D. Flemming
- Nature Structural & Molecular Biology, Vol. 15, Issue 8
Histone chaperones in nucleosome assembly and human disease
journal, January 2013
- Burgess, Rebecca J.; Zhang, Zhiguo
- Nature Structural & Molecular Biology, Vol. 20, Issue 1
Chromatin assembly: Cooperation between histone chaperones and ATP-dependent nucleosome remodeling machines
journal, April 2002
- Tyler, Jessica K.
- European Journal of Biochemistry, Vol. 269, Issue 9
Nucleoplasmin Binds Histone H2A-H2B Dimers through Its Distal Face
journal, August 2010
- Ramos, Isbaal; Martín-Benito, Jaime; Finn, Ron
- Journal of Biological Chemistry, Vol. 285, Issue 44
Interaction of Nucleoplasmin with Core Histones
journal, June 2003
- Arnan, Carme; Saperas, Núria; Prieto, Cèlia
- Journal of Biological Chemistry, Vol. 278, Issue 33
Phosphorylation of Both Nucleoplasmin Domains Is Required for Activation of Its Chromatin Decondensation Activity
journal, May 2007
- Bañuelos, Sonia; Omaetxebarria, Miren J.; Ramos, Isbaal
- Journal of Biological Chemistry, Vol. 282, Issue 29
Polyglutamylation Is a Post-translational Modification with a Broad Range of Substrates
journal, November 2007
- van Dijk, Juliette; Miro, Julie; Strub, Jean-Marc
- Journal of Biological Chemistry, Vol. 283, Issue 7
Flexible-meccano: a tool for the generation of explicit ensemble descriptions of intrinsically disordered proteins and their associated experimental observables
journal, May 2012
- Ozenne, V.; Bauer, F.; Salmon, L.
- Bioinformatics, Vol. 28, Issue 11
The human histone chaperone sNASP interacts with linker and core histones through distinct mechanisms
journal, September 2011
- Wang, H.; Ge, Z.; Walsh, S. T. R.
- Nucleic Acids Research, Vol. 40, Issue 2
Intrinsically disordered regions of nucleophosmin/B23 regulate its RNA binding activity through their inter- and intra-molecular association
journal, October 2013
- Hisaoka, M.; Nagata, K.; Okuwaki, M.
- Nucleic Acids Research, Vol. 42, Issue 2
The intrinsically disordered distal face of nucleoplasmin recognizes distinct oligomerization states of histones
journal, October 2013
- Ramos, I.; Fernandez-Rivero, N.; Arranz, R.
- Nucleic Acids Research, Vol. 42, Issue 2
An integrated high-throughput data acquisition system for biological solution X-ray scattering studies
journal, March 2012
- Martel, Anne; Liu, Ping; Weiss, Thomas M.
- Journal of Synchrotron Radiation, Vol. 19, Issue 3
Ambiguity assessment of small-angle scattering curves from monodisperse systems
journal, April 2015
- Petoukhov, Maxim V.; Svergun, Dmitri I.
- Acta Crystallographica Section D Biological Crystallography, Vol. 71, Issue 5
C-terminal acidic domain of histone chaperone human NAP1 is an efficient binding assistant for histone H2A-H2B, but not H3-H4
journal, February 2016
- Ohtomo, Hideaki; Akashi, Satoko; Moriwaki, Yoshihito
- Genes to Cells, Vol. 21, Issue 3
Roles of NPM2 in Chromatin and Nucleolar Organization in Oocytes and Embryos
journal, April 2003
- Burns, K. H.
- Science, Vol. 300, Issue 5619
Probabilistic Interaction Network of Evidence Algorithm and its Application to Complete Labeling of Peak Lists from Protein NMR Spectroscopy
journal, March 2009
- Bahrami, Arash; Assadi, Amir H.; Markley, John L.
- PLoS Computational Biology, Vol. 5, Issue 3
DAMMIF, a program for rapid ab-initio shape determination in small-angle scattering
text, January 2009
- Franke, D.; Svergun, D. I.
- Deutsches Elektronen-Synchrotron, DESY, Hamburg
Works referencing / citing this record:
Structure-function relationship of H2A-H2B specific plant histone chaperones
journal, November 2019
- Kumar, Ashish; Vasudevan, Dileep
- Cell Stress and Chaperones, Vol. 25, Issue 1
Structural insights into the ability of nucleoplasmin to assemble and chaperone histone octamers for DNA deposition
journal, July 2019
- Franco, Aitor; Arranz, Rocío; Fernández-Rivero, Noelia
- Scientific Reports, Vol. 9, Issue 1
Newly developed Laboratory-based Size exclusion chromatography Small-angle x-ray scattering System (La-SSS)
journal, August 2019
- Inoue, Rintaro; Nakagawa, Tatsuo; Morishima, Ken
- Scientific Reports, Vol. 9, Issue 1
Nucleoplasmin is a limiting component in the scaling of nuclear size with cytoplasmic volume
journal, October 2019
- Chen, Pan; Tomschik, Miroslav; Nelson, Katherine M.
- Journal of Cell Biology, Vol. 218, Issue 12
AtFKBP53: a chimeric histone chaperone with functional nucleoplasmin and PPIase domains
journal, December 2019
- Singh, Ajit Kumar; Datta, Aritreyee; Jobichen, Chacko
- Nucleic Acids Research, Vol. 48, Issue 3
The Transcriptional Roles of ALK Fusion Proteins in Tumorigenesis
journal, July 2019
- Ducray, Stephen P.; Natarajan, Karthikraj; Garland, Gavin D.
- Cancers, Vol. 11, Issue 8
The Transcriptional Roles of ALK Fusion Proteins in Tumorigenesis.
text, January 2019
- Ducray, Stephen; Natarajan, Karthikraj; Garland, Gavin D.
- Apollo - University of Cambridge Repository
Structural insights into the ability of nucleoplasmin to assemble and chaperone histone octamers for DNA deposition
journal, July 2019
- Franco, Aitor; Arranz, Rocío; Fernández-Rivero, Noelia
- Scientific Reports, Vol. 9, Issue 1
Newly developed Laboratory-based Size exclusion chromatography Small-angle x-ray scattering System (La-SSS)
journal, August 2019
- Inoue, Rintaro; Nakagawa, Tatsuo; Morishima, Ken
- Scientific Reports, Vol. 9, Issue 1
AtFKBP53: a chimeric histone chaperone with functional nucleoplasmin and PPIase domains
journal, December 2019
- Singh, Ajit Kumar; Datta, Aritreyee; Jobichen, Chacko
- Nucleic Acids Research, Vol. 48, Issue 3
The Transcriptional Roles of ALK Fusion Proteins in Tumorigenesis.
text, January 2019
- Ducray, Stephen; Natarajan, Karthikraj; Garland, Gavin D.
- Apollo - University of Cambridge Repository