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Title: Metagenomic analysis of intertidal hypersaline microbial mats from Elkhorn Slough, California, grown with and without molybdate

Cyanobacterial mats are laminated microbial ecosystems which occur in highly diverse environments and which may provide a possible model for early life on Earth. Their ability to produce hydrogen also makes them of interest from a biotechnological and bioenergy perspective. Samples of an intertidal microbial mat from the Elkhorn Slough estuary in Monterey Bay, California, were transplanted to a greenhouse at NASA Ames Research Center to study a 24-h diel cycle, in the presence or absence of molybdate (which inhibits biohydrogen consumption by sulfate reducers). Here, we present metagenomic analyses of four samples that will be used as references for future metatranscriptomic analyses of this diel time series.
Authors:
 [1] ;  [2] ;  [2] ;  [3] ;  [4] ;  [1] ;  [5] ;  [5] ;  [6] ;  [6] ;  [2] ;  [1]
  1. Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
  2. NASA Ames Research Center (ARC), Moffett Field, Mountain View, CA (United States)
  3. NASA Ames Research Center (ARC), Moffett Field, Mountain View, CA (United States); Stanford Univ., Stanford, CA (United States)
  4. NASA Ames Research Center (ARC), Moffett Field, Mountain View, CA (United States); Bay Area Environmental Research Inst., Petaluma, CA (United States)
  5. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
  6. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States)
Publication Date:
Report Number(s):
LLNL-JRNL-739283
Journal ID: ISSN 1944-3277; ark:/13030/qt8ng6c7p0
Grant/Contract Number:
AC02-05CH11231; FWP SCW1039; 701; AC52-07NA27344
Type:
Accepted Manuscript
Journal Name:
Standards in Genomic Sciences
Additional Journal Information:
Journal Volume: 12; Journal Issue: 1; Journal ID: ISSN 1944-3277
Publisher:
BioMed Central
Research Org:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
Sponsoring Org:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Microbial mats; Hydrogen; Fermentation; Elkhorn slough; Metagenomics; Environmental sciences, Biological and medical sciences, Chemistry
OSTI Identifier:
1419443
Alternate Identifier(s):
OSTI ID: 1467807