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Title: Transcriptomic profiles of Clostridium ljungdahlii during lithotrophic growth with syngas or H 2 and CO 2 compared to organotrophic growth with fructose

Clostridium ljungdahlii derives energy by lithotrophic and organotrophic acetogenesis. C. ljungdahlii was grown organotrophically with fructose and also lithotrophically, either with syngas - a gas mixture containing hydrogen (H 2), carbon dioxide (CO 2), and carbon monoxide (CO), or with H 2 and CO 2. Gene expression was compared quantitatively by microarrays using RNA extracted from all three conditions. Gene expression with fructose and with H 2/CO 2 was compared by RNA-Seq. Upregulated genes with both syngas and H 2/CO 2 (compared to fructose) point to the urea cycle, uptake and degradation of peptides and amino acids, response to sulfur starvation, potentially NADPH-producing pathways involving (S)-malate and ornithine, quorum sensing, sporulation, and cell wall remodeling, suggesting a global and multicellular response to lithotrophic conditions. With syngas, the upregulated (R)-lactate dehydrogenase gene represents a route of electron transfer from ferredoxin to NAD. With H 2/CO 2, flavodoxin and histidine biosynthesis genes were upregulated. Downregulated genes corresponded to an intracytoplasmic microcompartment for disposal of methylglyoxal, a toxic byproduct of glycolysis, as 1-propanol. Several cytoplasmic and membrane-associated redox-active protein genes were differentially regulated. In conclusion, the transcriptomic profiles of C. ljungdahlii in lithotrophic and organotrophic growth modes indicate large-scale physiological and metabolic differences,more » observations that may guide biofuel and commodity chemical production with this species.« less
Authors:
 [1] ;  [2] ;  [3] ;  [4] ;  [5]
  1. Univ. of Massachusetts, Amherst, MA (United States); Univ. of Nevada, Reno, NV (United States)
  2. Univ. of Massachusetts, Amherst, MA (United States); LanzaTech, Skokie, IL (United States)
  3. Univ. of Massachusetts, Amherst, MA (United States); Univ. of California, Berkeley, CA (United States). Energy Biosciences Inst.
  4. Univ. of Massachusetts, Amherst, MA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Earth and Environmental Sciences
  5. Univ. of Massachusetts, Amherst, MA (United States)
Publication Date:
Grant/Contract Number:
AC02-05CH11231; AR0000159; N000141310549
Type:
Accepted Manuscript
Journal Name:
Scientific Reports
Additional Journal Information:
Journal Volume: 7; Journal Issue: 1; Journal ID: ISSN 2045-2322
Publisher:
Nature Publishing Group
Research Org:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org:
USDOE Advanced Research Projects Agency - Energy (ARPA-E); US Department of the Navy, Office of Naval Research (ONR)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Bacterial genomics; Gene expression profiling
OSTI Identifier:
1419439