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Title: SWEET11 and 15 as key players in seed filling in rice

Abstract

Despite the relevance of seed-filling mechanisms for crop yield, we still have only a rudimentary understanding of the transport processes that supply the caryopsis with sugars. Here, we hypothesized that SWEET sucrose transporters may play important roles in nutrient import pathways in the rice caryopsis. We used a combination of mRNA quantification, histochemical analyses, translational promoter–reporter fusions and analysis of knockout mutants created by genomic editing to evaluate the contribution of SWEET transporters to seed filling. In rice caryopses, SWEET11 and 15 had the highest mRNA levels and proteins localized to four key sites: all regions of the nucellus at early stages; the nucellar projection close to the dorsal vein; the nucellar epidermis that surrounds the endosperm; and the aleurone. ossweet11;15 double knockout lines accumulated starch in the pericarp, whereas caryopses did not contain a functional endosperm. Jointly, SWEET11 and 15 show all the hallmarks of being necessary for seed filling with sucrose efflux functions at the nucellar projection and a role in transfer across the nucellar epidermis/aleurone interface, delineating two major steps for apoplasmic seed filling, observations that are discussed in relation to observations made in rice and barley regarding the relative prevalence of these two potential import routes.

Authors:
 [1];  [2];  [2];  [3];  [1]
  1. Institute for Molecular Physiology, Heinrich-Heine University Duesseldorf, 40225 Duesseldorf Germany, Max Planck Institute for Plant Breeding Research, 50829 Cologne Germany, Department of Plant Biology, Carnegie Institution for Science, Stanford CA 94305 USA
  2. Department of Genetics, Development, and Cell Biology, Iowa State University, Ames IA 50011 USA
  3. Institute for Molecular Physiology, Heinrich-Heine University Duesseldorf, 40225 Duesseldorf Germany, Max Planck Institute for Plant Breeding Research, 50829 Cologne Germany, Department of Plant Biology, Carnegie Institution for Science, Stanford CA 94305 USA, Institute of Transformative Bio-Molecules (ITbM), Nagoya University, Furo-cho, Chikusa-ku Nagoya Aichi 464-8602 Japan
Publication Date:
Research Org.:
Carnegie Inst. of Washington, Washington, DC (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1419360
Alternate Identifier(s):
OSTI ID: 1419361; OSTI ID: 1500104
Grant/Contract Number:  
FG02-04ER15542
Resource Type:
Published Article
Journal Name:
New Phytologist
Additional Journal Information:
Journal Name: New Phytologist Journal Volume: 218 Journal Issue: 2; Journal ID: ISSN 0028-646X
Publisher:
Wiley-Blackwell
Country of Publication:
United Kingdom
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; apoplasmic pathway; caryopsis; endosperm; rice; sucrose transporter; SWEET

Citation Formats

Yang, Jungil, Luo, Dangping, Yang, Bing, Frommer, Wolf B., and Eom, Joon-Seob. SWEET11 and 15 as key players in seed filling in rice. United Kingdom: N. p., 2018. Web. doi:10.1111/nph.15004.
Yang, Jungil, Luo, Dangping, Yang, Bing, Frommer, Wolf B., & Eom, Joon-Seob. SWEET11 and 15 as key players in seed filling in rice. United Kingdom. doi:10.1111/nph.15004.
Yang, Jungil, Luo, Dangping, Yang, Bing, Frommer, Wolf B., and Eom, Joon-Seob. Fri . "SWEET11 and 15 as key players in seed filling in rice". United Kingdom. doi:10.1111/nph.15004.
@article{osti_1419360,
title = {SWEET11 and 15 as key players in seed filling in rice},
author = {Yang, Jungil and Luo, Dangping and Yang, Bing and Frommer, Wolf B. and Eom, Joon-Seob},
abstractNote = {Despite the relevance of seed-filling mechanisms for crop yield, we still have only a rudimentary understanding of the transport processes that supply the caryopsis with sugars. Here, we hypothesized that SWEET sucrose transporters may play important roles in nutrient import pathways in the rice caryopsis. We used a combination of mRNA quantification, histochemical analyses, translational promoter–reporter fusions and analysis of knockout mutants created by genomic editing to evaluate the contribution of SWEET transporters to seed filling. In rice caryopses, SWEET11 and 15 had the highest mRNA levels and proteins localized to four key sites: all regions of the nucellus at early stages; the nucellar projection close to the dorsal vein; the nucellar epidermis that surrounds the endosperm; and the aleurone. ossweet11;15 double knockout lines accumulated starch in the pericarp, whereas caryopses did not contain a functional endosperm. Jointly, SWEET11 and 15 show all the hallmarks of being necessary for seed filling with sucrose efflux functions at the nucellar projection and a role in transfer across the nucellar epidermis/aleurone interface, delineating two major steps for apoplasmic seed filling, observations that are discussed in relation to observations made in rice and barley regarding the relative prevalence of these two potential import routes.},
doi = {10.1111/nph.15004},
journal = {New Phytologist},
number = 2,
volume = 218,
place = {United Kingdom},
year = {2018},
month = {2}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
DOI: 10.1111/nph.15004

Citation Metrics:
Cited by: 4 works
Citation information provided by
Web of Science

Figures / Tables:

Fig. 1 Fig. 1: Relative expression of OsSWEET4, OsSWEET11 and OsSWEET15 during rice seed development after pollination. Expression was measured by quantitative reverse transcription polymerase chain reaction (qRT-PCR) in wild-type glasshouse-grown seeds. Data shown as mean ± SEM, n = 3; expression levels were normalized to rice Ubiquitin1 levels.

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    Works referencing / citing this record:

    CRISPR/Cas9-targeted mutagenesis of Os8N3 in rice to confer resistance to Xanthomonas oryzae pv. oryzae
    journal, August 2019


    Common metabolic networks contribute to carbon sink strength of sorghum internodes: implications for bioenergy improvement
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    CRISPR/Cas9-targeted mutagenesis of Os8N3 in rice to confer resistance to Xanthomonas oryzae pv. oryzae
    journal, August 2019


    Common metabolic networks contribute to carbon sink strength of sorghum internodes: implications for bioenergy improvement
    journal, November 2019


      Figures/Tables have been extracted from DOE-funded journal article accepted manuscripts.