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Title: Change in lignin structure, but not in lignin content, in transgenic poplar overexpressing the rice master regulator of secondary cell wall biosynthesis

Abstract

We previously succeeded in enhancing wood formation of wood in transgenic poplar plants by overexpressing secondary wall NAM/ATAF/CUC (NAC) domain protein 1 from Oryza sativa ( OsSWN1 ), a transcription factor ‘master regulator’ of secondary cell wall formation in rice, under control of the fiber preferential NST3/SND1 promoter from Arabidopsis. Transgenic plants had an increased cell wall thickness and cell wall density of individual cells in the secondary xylem of stems as well as an increased wood density. OsSWN1 triggers the induction of polysaccharide and lignin biosynthetic gene expressions, however, resulting in no significant impact on the lignin content in the transgenic plants. In contrast, wet and dry chemical analyses of lignin revealed changes in S/G ratio and in the composition of lignin interunit linkages in transgenic lines. The results from gene expression analysis suggest that the structural changes in lignin were due to an unbalanced induction of lignin biosynthetic genes in transgenic lines. Our present data indicate that the overexpression of the chimeric transcription factor causes accelerated deposition of secondary cell wall components including lignin and polysaccharides through an acquired mechanism.

Authors:
 [1];  [2];  [3];  [4];  [5];  [3];  [2];  [4]; ORCiD logo [1]
  1. Graduate School of Bio‐Applications and Systems Engineering Tokyo University of Agriculture and Technology, Koganei Tokyo 184‐8588 Japan
  2. US Department of Energy, Great Lakes Bioenergy Research Center Wisconsin Energy Institute, University of Wisconsin Madison WI 53726 USA, Department of Biochemistry University of Wisconsin Madison WI 53706 USA
  3. Forest Bio‐Research Center Forestry and Forest Products Research Institute, Forest Research and Management Organization, Hitachi Ibaraki 319‐1301 Japan
  4. Bioproduction Research Institute National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Ibaraki 305‐8566 Japan
  5. Graduate School of Life and Environmental Sciences University of Tsukuba, Tsukuba Ibaraki 305‐8572 Japan
Publication Date:
Sponsoring Org.:
USDOE
OSTI Identifier:
1419090
Grant/Contract Number:  
DE‐FC02‐07ER64494
Resource Type:
Publisher's Accepted Manuscript
Journal Name:
Physiologia Plantarum
Additional Journal Information:
Journal Name: Physiologia Plantarum Journal Volume: 163 Journal Issue: 2; Journal ID: ISSN 0031-9317
Publisher:
Wiley-Blackwell
Country of Publication:
Denmark
Language:
English

Citation Formats

Nuoendagula,, Tsuji, Yukiko, Takata, Naoki, Sakamoto, Shingo, Nakagawa‐Izumi, Akiko, Taniguchi, Toru, Ralph, John, Mitsuda, Nobutaka, and Kajita, Shinya. Change in lignin structure, but not in lignin content, in transgenic poplar overexpressing the rice master regulator of secondary cell wall biosynthesis. Denmark: N. p., 2018. Web. doi:10.1111/ppl.12684.
Nuoendagula,, Tsuji, Yukiko, Takata, Naoki, Sakamoto, Shingo, Nakagawa‐Izumi, Akiko, Taniguchi, Toru, Ralph, John, Mitsuda, Nobutaka, & Kajita, Shinya. Change in lignin structure, but not in lignin content, in transgenic poplar overexpressing the rice master regulator of secondary cell wall biosynthesis. Denmark. https://doi.org/10.1111/ppl.12684
Nuoendagula,, Tsuji, Yukiko, Takata, Naoki, Sakamoto, Shingo, Nakagawa‐Izumi, Akiko, Taniguchi, Toru, Ralph, John, Mitsuda, Nobutaka, and Kajita, Shinya. Thu . "Change in lignin structure, but not in lignin content, in transgenic poplar overexpressing the rice master regulator of secondary cell wall biosynthesis". Denmark. https://doi.org/10.1111/ppl.12684.
@article{osti_1419090,
title = {Change in lignin structure, but not in lignin content, in transgenic poplar overexpressing the rice master regulator of secondary cell wall biosynthesis},
author = {Nuoendagula, and Tsuji, Yukiko and Takata, Naoki and Sakamoto, Shingo and Nakagawa‐Izumi, Akiko and Taniguchi, Toru and Ralph, John and Mitsuda, Nobutaka and Kajita, Shinya},
abstractNote = {We previously succeeded in enhancing wood formation of wood in transgenic poplar plants by overexpressing secondary wall NAM/ATAF/CUC (NAC) domain protein 1 from Oryza sativa ( OsSWN1 ), a transcription factor ‘master regulator’ of secondary cell wall formation in rice, under control of the fiber preferential NST3/SND1 promoter from Arabidopsis. Transgenic plants had an increased cell wall thickness and cell wall density of individual cells in the secondary xylem of stems as well as an increased wood density. OsSWN1 triggers the induction of polysaccharide and lignin biosynthetic gene expressions, however, resulting in no significant impact on the lignin content in the transgenic plants. In contrast, wet and dry chemical analyses of lignin revealed changes in S/G ratio and in the composition of lignin interunit linkages in transgenic lines. The results from gene expression analysis suggest that the structural changes in lignin were due to an unbalanced induction of lignin biosynthetic genes in transgenic lines. Our present data indicate that the overexpression of the chimeric transcription factor causes accelerated deposition of secondary cell wall components including lignin and polysaccharides through an acquired mechanism.},
doi = {10.1111/ppl.12684},
journal = {Physiologia Plantarum},
number = 2,
volume = 163,
place = {Denmark},
year = {Thu Feb 01 00:00:00 EST 2018},
month = {Thu Feb 01 00:00:00 EST 2018}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
https://doi.org/10.1111/ppl.12684

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Cited by: 15 works
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