Change in lignin structure, but not in lignin content, in transgenic poplar overexpressing the rice master regulator of secondary cell wall biosynthesis
Abstract
We previously succeeded in enhancing wood formation of wood in transgenic poplar plants by overexpressing secondary wall NAM/ATAF/CUC (NAC) domain protein 1 from Oryza sativa ( OsSWN1 ), a transcription factor ‘master regulator’ of secondary cell wall formation in rice, under control of the fiber preferential NST3/SND1 promoter from Arabidopsis. Transgenic plants had an increased cell wall thickness and cell wall density of individual cells in the secondary xylem of stems as well as an increased wood density. OsSWN1 triggers the induction of polysaccharide and lignin biosynthetic gene expressions, however, resulting in no significant impact on the lignin content in the transgenic plants. In contrast, wet and dry chemical analyses of lignin revealed changes in S/G ratio and in the composition of lignin interunit linkages in transgenic lines. The results from gene expression analysis suggest that the structural changes in lignin were due to an unbalanced induction of lignin biosynthetic genes in transgenic lines. Our present data indicate that the overexpression of the chimeric transcription factor causes accelerated deposition of secondary cell wall components including lignin and polysaccharides through an acquired mechanism.
- Authors:
-
- Graduate School of Bio‐Applications and Systems Engineering Tokyo University of Agriculture and Technology, Koganei Tokyo 184‐8588 Japan
- US Department of Energy, Great Lakes Bioenergy Research Center Wisconsin Energy Institute, University of Wisconsin Madison WI 53726 USA, Department of Biochemistry University of Wisconsin Madison WI 53706 USA
- Forest Bio‐Research Center Forestry and Forest Products Research Institute, Forest Research and Management Organization, Hitachi Ibaraki 319‐1301 Japan
- Bioproduction Research Institute National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Ibaraki 305‐8566 Japan
- Graduate School of Life and Environmental Sciences University of Tsukuba, Tsukuba Ibaraki 305‐8572 Japan
- Publication Date:
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1419090
- Grant/Contract Number:
- DE‐FC02‐07ER64494
- Resource Type:
- Publisher's Accepted Manuscript
- Journal Name:
- Physiologia Plantarum
- Additional Journal Information:
- Journal Name: Physiologia Plantarum Journal Volume: 163 Journal Issue: 2; Journal ID: ISSN 0031-9317
- Publisher:
- Wiley-Blackwell
- Country of Publication:
- Denmark
- Language:
- English
Citation Formats
Nuoendagula,, Tsuji, Yukiko, Takata, Naoki, Sakamoto, Shingo, Nakagawa‐Izumi, Akiko, Taniguchi, Toru, Ralph, John, Mitsuda, Nobutaka, and Kajita, Shinya. Change in lignin structure, but not in lignin content, in transgenic poplar overexpressing the rice master regulator of secondary cell wall biosynthesis. Denmark: N. p., 2018.
Web. doi:10.1111/ppl.12684.
Nuoendagula,, Tsuji, Yukiko, Takata, Naoki, Sakamoto, Shingo, Nakagawa‐Izumi, Akiko, Taniguchi, Toru, Ralph, John, Mitsuda, Nobutaka, & Kajita, Shinya. Change in lignin structure, but not in lignin content, in transgenic poplar overexpressing the rice master regulator of secondary cell wall biosynthesis. Denmark. https://doi.org/10.1111/ppl.12684
Nuoendagula,, Tsuji, Yukiko, Takata, Naoki, Sakamoto, Shingo, Nakagawa‐Izumi, Akiko, Taniguchi, Toru, Ralph, John, Mitsuda, Nobutaka, and Kajita, Shinya. Thu .
"Change in lignin structure, but not in lignin content, in transgenic poplar overexpressing the rice master regulator of secondary cell wall biosynthesis". Denmark. https://doi.org/10.1111/ppl.12684.
@article{osti_1419090,
title = {Change in lignin structure, but not in lignin content, in transgenic poplar overexpressing the rice master regulator of secondary cell wall biosynthesis},
author = {Nuoendagula, and Tsuji, Yukiko and Takata, Naoki and Sakamoto, Shingo and Nakagawa‐Izumi, Akiko and Taniguchi, Toru and Ralph, John and Mitsuda, Nobutaka and Kajita, Shinya},
abstractNote = {We previously succeeded in enhancing wood formation of wood in transgenic poplar plants by overexpressing secondary wall NAM/ATAF/CUC (NAC) domain protein 1 from Oryza sativa ( OsSWN1 ), a transcription factor ‘master regulator’ of secondary cell wall formation in rice, under control of the fiber preferential NST3/SND1 promoter from Arabidopsis. Transgenic plants had an increased cell wall thickness and cell wall density of individual cells in the secondary xylem of stems as well as an increased wood density. OsSWN1 triggers the induction of polysaccharide and lignin biosynthetic gene expressions, however, resulting in no significant impact on the lignin content in the transgenic plants. In contrast, wet and dry chemical analyses of lignin revealed changes in S/G ratio and in the composition of lignin interunit linkages in transgenic lines. The results from gene expression analysis suggest that the structural changes in lignin were due to an unbalanced induction of lignin biosynthetic genes in transgenic lines. Our present data indicate that the overexpression of the chimeric transcription factor causes accelerated deposition of secondary cell wall components including lignin and polysaccharides through an acquired mechanism.},
doi = {10.1111/ppl.12684},
journal = {Physiologia Plantarum},
number = 2,
volume = 163,
place = {Denmark},
year = {Thu Feb 01 00:00:00 EST 2018},
month = {Thu Feb 01 00:00:00 EST 2018}
}
https://doi.org/10.1111/ppl.12684
Web of Science
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