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Title: Surface-Induced Dissociation of Protein Complexes in a Hybrid Fourier Transform Ion Cyclotron Resonance Mass Spectrometer

Abstract

Mass spectrometry continues to develop as a valuable tool in the analysis of proteins and protein complexes. In protein complex mass spectrometry studies, surface-induced dissociation (SID) has been successfully applied in quadrupole time-of-flight (Q-TOF) instruments. SID provides structural information on noncovalent protein complexes that is complementary to other techniques. However, the mass resolution of Q-TOF instruments can limit the information that can be obtained for protein complexes by SID. Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) provides ultrahigh resolution and ultrahigh mass accuracy measurements. Here in this study, an SID device was designed and successfully installed in a hybrid FT-ICR instrument in place of the standard gas collision cell. The SID-FT-ICR platform has been tested with several protein complex systems (homooligomers, a heterooligomer, and a protein-ligand complex, ranging from 53 to 85 kDa), and the results are consistent with data previously acquired on Q-TOF platforms, matching predictions from known protein interface information. Lastly, SID fragments with the same m/z but different charge states are well-resolved based on distinct spacing between adjacent isotope peaks, and the addition of metal cations and ligands can also be isotopically resolved with the ultrahigh mass resolution available in FT-ICR.

Authors:
ORCiD logo [1]; ORCiD logo [2];  [1];  [3];  [4];  [5];  [1];  [1];  [3];  [2];  [1]
+ Show Author Affiliations
  1. The Ohio State Univ., Columbus, OH (United States). Dept. of Chemistry and Biochemistry
  2. Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Environmental Molecular Sciences Lab. (EMSL)
  3. Bruker Corp., Billerica, MA (United States)
  4. The Ohio State Univ., Columbus, OH (United States). OSU Mass Spectrometry and Proteomics Facility
  5. Ardara Technologies L.P., Ardara, PA (United States)
Publication Date:
Research Org.:
Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)
Sponsoring Org.:
National Science Foundation (NSF); USDOE Office of Science (SC), Biological and Environmental Research (BER); National Institutes of Health (NIH)
OSTI Identifier:
1418480
Resource Type:
Accepted Manuscript
Journal Name:
Analytical Chemistry
Additional Journal Information:
Journal Volume: 89; Journal Issue: 1; Journal ID: ISSN 0003-2700
Publisher:
American Chemical Society (ACS)
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY

Citation Formats

Yan, Jing, Zhou, Mowei, Gilbert, Joshua D., Wolff, Jeremy J., Somogyi, Arpad, Pedder, Randall E., Quintyn, Royston S., Morrison, Lindsay J., Easterling, Michael L., Pasa-Tolic, Ljiljana, and Wysocki, Vicki H. Surface-Induced Dissociation of Protein Complexes in a Hybrid Fourier Transform Ion Cyclotron Resonance Mass Spectrometer. United States: N. p., 2016. Web. doi:10.1021/acs.analchem.6b03986.
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Yan, Jing, Zhou, Mowei, Gilbert, Joshua D., Wolff, Jeremy J., Somogyi, Arpad, Pedder, Randall E., Quintyn, Royston S., Morrison, Lindsay J., Easterling, Michael L., Pasa-Tolic, Ljiljana, & Wysocki, Vicki H. Surface-Induced Dissociation of Protein Complexes in a Hybrid Fourier Transform Ion Cyclotron Resonance Mass Spectrometer. United States. https://doi.org/10.1021/acs.analchem.6b03986
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Yan, Jing, Zhou, Mowei, Gilbert, Joshua D., Wolff, Jeremy J., Somogyi, Arpad, Pedder, Randall E., Quintyn, Royston S., Morrison, Lindsay J., Easterling, Michael L., Pasa-Tolic, Ljiljana, and Wysocki, Vicki H. Fri . "Surface-Induced Dissociation of Protein Complexes in a Hybrid Fourier Transform Ion Cyclotron Resonance Mass Spectrometer". United States. https://doi.org/10.1021/acs.analchem.6b03986. https://www.osti.gov/servlets/purl/1418480.
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@article{osti_1418480,
title = {Surface-Induced Dissociation of Protein Complexes in a Hybrid Fourier Transform Ion Cyclotron Resonance Mass Spectrometer},
author = {Yan, Jing and Zhou, Mowei and Gilbert, Joshua D. and Wolff, Jeremy J. and Somogyi, Arpad and Pedder, Randall E. and Quintyn, Royston S. and Morrison, Lindsay J. and Easterling, Michael L. and Pasa-Tolic, Ljiljana and Wysocki, Vicki H.},
abstractNote = {Mass spectrometry continues to develop as a valuable tool in the analysis of proteins and protein complexes. In protein complex mass spectrometry studies, surface-induced dissociation (SID) has been successfully applied in quadrupole time-of-flight (Q-TOF) instruments. SID provides structural information on noncovalent protein complexes that is complementary to other techniques. However, the mass resolution of Q-TOF instruments can limit the information that can be obtained for protein complexes by SID. Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) provides ultrahigh resolution and ultrahigh mass accuracy measurements. Here in this study, an SID device was designed and successfully installed in a hybrid FT-ICR instrument in place of the standard gas collision cell. The SID-FT-ICR platform has been tested with several protein complex systems (homooligomers, a heterooligomer, and a protein-ligand complex, ranging from 53 to 85 kDa), and the results are consistent with data previously acquired on Q-TOF platforms, matching predictions from known protein interface information. Lastly, SID fragments with the same m/z but different charge states are well-resolved based on distinct spacing between adjacent isotope peaks, and the addition of metal cations and ligands can also be isotopically resolved with the ultrahigh mass resolution available in FT-ICR.},
doi = {10.1021/acs.analchem.6b03986},
journal = {Analytical Chemistry},
number = 1,
volume = 89,
place = {United States},
year = {Fri Dec 02 00:00:00 EST 2016},
month = {Fri Dec 02 00:00:00 EST 2016}
}
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https://doi.org/10.1021/acs.analchem.6b03986
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    Radical solutions: Principles and application of electron-based dissociation in mass spectrometry-based analysis of protein structure
    journal, February 2018

    • Lermyte, Frederik; Valkenborg, Dirk; Loo, Joseph A.
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    • DOI: 10.1002/mas.21560

    Surface Induced Dissociation Coupled with High Resolution Mass Spectrometry Unveils Heterogeneity of a 211 kDa Multicopper Oxidase Protein Complex
    journal, January 2018

    • Zhou, Mowei; Yan, Jing; Romano, Christine A.
    • Journal of The American Society for Mass Spectrometry, Vol. 29, Issue 4
    • DOI: 10.1007/s13361-017-1882-x

    Experimental and theoretical investigation of overall energy deposition in surface-induced unfolding of protein ions
    journal, January 2019

    • Donor, Micah T.; Mroz, Austin M.; Prell, James S.
    • Chemical Science, Vol. 10, Issue 14
    • DOI: 10.1039/c9sc00644c

    Experimental and theoretical investigation of overall energy deposition in surface-induced unfolding of protein ions
    journal, January 2019

    • Donor, Micah T.; Mroz, Austin M.; Prell, James S.
    • Chemical Science, Vol. 10, Issue 14
    • DOI: 10.1039/c9sc00644c
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