Femtosecond X-ray diffraction from an aerosolized beam of protein nanocrystals
- Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany). Center for Free-Electron Laser Science; Univ. of Hamburg (Germany). Hamburg Center for Ultrafast Imaging
- Arizona State Univ., Tempe, AZ (United States)
- Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany). Center for Free-Electron Laser Science; Univ. of Hamburg (Germany). Dept. of Physics
- Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany). Center for Free-Electron Laser Science
- Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany). Photon Science
- Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany). Center for Free-Electron Laser Science; Max-Planck Inst. for the Structure and Dynamics of Matter, Hamburg (Germany)
- SLAC National Accelerator Lab., Menlo Park, CA (United States). Linac Coherent Light Source (LCLS)
- Australian National Univ., Canberra, ACT (Australia). Laser Physics Centre, Research School of Physics and Engineering
- Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany). Center for Free-Electron Laser Science; Univ. of Hamburg (Germany). Hamburg Center for Ultrafast Imaging; Univ. of Hamburg (Germany). Dept. of Physics
High-resolution Bragg diffraction from aerosolized single granulovirus nanocrystals using an X-ray free-electron laser is demonstrated. The outer dimensions of the in-vacuum aerosol injector components are identical to conventional liquid-microjet nozzles used in serial diffraction experiments, which allows the injector to be utilized with standard mountings. As compared with liquid-jet injection, the X-ray scattering background is reduced by several orders of magnitude by the use of helium carrier gas rather than liquid. Such reduction is required for diffraction measurements of small macromolecular nanocrystals and single particles. High particle speeds are achieved, making the approach suitable for use at upcoming high-repetition-rate facilities.
- Research Organization:
- SLAC National Accelerator Laboratory (SLAC), Menlo Park, CA (United States)
- Sponsoring Organization:
- USDOE Office of Science (SC), Basic Energy Sciences (BES); National Institutes of Health (NIH); European Union (EU); National Science Foundation (NSF); German Research Foundation (DFG); USDOE
- Grant/Contract Number:
- AC02-76SF00515; P41GM103393; P41RR001209; ERC-Kupper-614507; 1231306
- OSTI ID:
- 1418182
- Alternate ID(s):
- OSTI ID: 1425348
- Journal Information:
- Journal of Applied Crystallography (Online), Vol. 51, Issue 1; ISSN 1600-5767
- Publisher:
- International Union of CrystallographyCopyright Statement
- Country of Publication:
- United States
- Language:
- English
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