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Title: Femtosecond X-ray diffraction from an aerosolized beam of protein nanocrystals

Abstract

High-resolution Bragg diffraction from aerosolized single granulovirus nanocrystals using an X-ray free-electron laser is demonstrated. The outer dimensions of the in-vacuum aerosol injector components are identical to conventional liquid-microjet nozzles used in serial diffraction experiments, which allows the injector to be utilized with standard mountings. As compared with liquid-jet injection, the X-ray scattering background is reduced by several orders of magnitude by the use of helium carrier gas rather than liquid. Such reduction is required for diffraction measurements of small macromolecular nanocrystals and single particles. High particle speeds are achieved, making the approach suitable for use at upcoming high-repetition-rate facilities.

Authors:
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Publication Date:
Research Org.:
SLAC National Accelerator Lab., Menlo Park, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22); National Institutes of Health (NIH); European Union (EU); National Science Foundation (NSF); German Research Foundation (DFG)
OSTI Identifier:
1418182
Alternate Identifier(s):
OSTI ID: 1425348
Grant/Contract Number:  
AC02-76SF00515; P41GM103393; P41RR001209; ERC-Kupper-614507; 1231306
Resource Type:
Published Article
Journal Name:
Journal of Applied Crystallography (Online)
Additional Journal Information:
Journal Name: Journal of Applied Crystallography (Online) Journal Volume: 51 Journal Issue: 1; Journal ID: ISSN 1600-5767
Publisher:
International Union of Crystallography (IUCr)
Country of Publication:
Denmark
Language:
English
Subject:
36 MATERIALS SCIENCE; 77 NANOSCIENCE AND NANOTECHNOLOGY

Citation Formats

Awel, Salah, Kirian, Richard A., Wiedorn, Max O., Beyerlein, Kenneth R., Roth, Nils, Horke, Daniel A., Oberthür, Dominik, Knoska, Juraj, Mariani, Valerio, Morgan, Andrew, Adriano, Luigi, Tolstikova, Alexandra, Xavier, P. Lourdu, Yefanov, Oleksandr, Aquila, Andrew, Barty, Anton, Roy-Chowdhury, Shatabdi, Hunter, Mark S., James, Daniel, Robinson, Joseph S., Weierstall, Uwe, Rode, Andrei V., Bajt, Saša, Küpper, Jochen, and Chapman, Henry N. Femtosecond X-ray diffraction from an aerosolized beam of protein nanocrystals. Denmark: N. p., 2018. Web. doi:10.1107/S1600576717018131.
Awel, Salah, Kirian, Richard A., Wiedorn, Max O., Beyerlein, Kenneth R., Roth, Nils, Horke, Daniel A., Oberthür, Dominik, Knoska, Juraj, Mariani, Valerio, Morgan, Andrew, Adriano, Luigi, Tolstikova, Alexandra, Xavier, P. Lourdu, Yefanov, Oleksandr, Aquila, Andrew, Barty, Anton, Roy-Chowdhury, Shatabdi, Hunter, Mark S., James, Daniel, Robinson, Joseph S., Weierstall, Uwe, Rode, Andrei V., Bajt, Saša, Küpper, Jochen, & Chapman, Henry N. Femtosecond X-ray diffraction from an aerosolized beam of protein nanocrystals. Denmark. doi:10.1107/S1600576717018131.
Awel, Salah, Kirian, Richard A., Wiedorn, Max O., Beyerlein, Kenneth R., Roth, Nils, Horke, Daniel A., Oberthür, Dominik, Knoska, Juraj, Mariani, Valerio, Morgan, Andrew, Adriano, Luigi, Tolstikova, Alexandra, Xavier, P. Lourdu, Yefanov, Oleksandr, Aquila, Andrew, Barty, Anton, Roy-Chowdhury, Shatabdi, Hunter, Mark S., James, Daniel, Robinson, Joseph S., Weierstall, Uwe, Rode, Andrei V., Bajt, Saša, Küpper, Jochen, and Chapman, Henry N. Thu . "Femtosecond X-ray diffraction from an aerosolized beam of protein nanocrystals". Denmark. doi:10.1107/S1600576717018131.
@article{osti_1418182,
title = {Femtosecond X-ray diffraction from an aerosolized beam of protein nanocrystals},
author = {Awel, Salah and Kirian, Richard A. and Wiedorn, Max O. and Beyerlein, Kenneth R. and Roth, Nils and Horke, Daniel A. and Oberthür, Dominik and Knoska, Juraj and Mariani, Valerio and Morgan, Andrew and Adriano, Luigi and Tolstikova, Alexandra and Xavier, P. Lourdu and Yefanov, Oleksandr and Aquila, Andrew and Barty, Anton and Roy-Chowdhury, Shatabdi and Hunter, Mark S. and James, Daniel and Robinson, Joseph S. and Weierstall, Uwe and Rode, Andrei V. and Bajt, Saša and Küpper, Jochen and Chapman, Henry N.},
abstractNote = {High-resolution Bragg diffraction from aerosolized single granulovirus nanocrystals using an X-ray free-electron laser is demonstrated. The outer dimensions of the in-vacuum aerosol injector components are identical to conventional liquid-microjet nozzles used in serial diffraction experiments, which allows the injector to be utilized with standard mountings. As compared with liquid-jet injection, the X-ray scattering background is reduced by several orders of magnitude by the use of helium carrier gas rather than liquid. Such reduction is required for diffraction measurements of small macromolecular nanocrystals and single particles. High particle speeds are achieved, making the approach suitable for use at upcoming high-repetition-rate facilities.},
doi = {10.1107/S1600576717018131},
journal = {Journal of Applied Crystallography (Online)},
number = 1,
volume = 51,
place = {Denmark},
year = {2018},
month = {2}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
DOI: 10.1107/S1600576717018131

Citation Metrics:
Cited by: 2 works
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Figures / Tables:

Figure 1 Figure 1: CNAI assembly and its operation during the CXI experiment. (a) Sketch of the basic aerosol generation and transportation setup. (b) The aerosol nozzle mounted on the nozzle rod. (c) Timeintegrated image of a laser-illuminated stream of GV particles exiting the CNAI, recorded using the inline microscope at themore » CXI instrument. This image was formed by averaging over 3.7 min, with a running median background subtracted from each frame. The CNAI tip is seen in the left portion of the image, and the approximate X-ray focal point is indicated by the star.« less

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      Figures/Tables have been extracted from DOE-funded journal article accepted manuscripts.