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Title: Femtosecond X-ray diffraction from an aerosolized beam of protein nanocrystals

High-resolution Bragg diffraction from aerosolized single granulovirus nanocrystals using an X-ray free-electron laser is demonstrated. The outer dimensions of the in-vacuum aerosol injector components are identical to conventional liquid-microjet nozzles used in serial diffraction experiments, which allows the injector to be utilized with standard mountings. As compared with liquid-jet injection, the X-ray scattering background is reduced by several orders of magnitude by the use of helium carrier gas rather than liquid. Such reduction is required for diffraction measurements of small macromolecular nanocrystals and single particles. High particle speeds are achieved, making the approach suitable for use at upcoming high-repetition-rate facilities.
Authors:
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Publication Date:
Grant/Contract Number:
AC02-76SF00515; P41GM103393; P41RR001209
Type:
Published Article
Journal Name:
Journal of Applied Crystallography
Additional Journal Information:
Journal Volume: 51; Journal Issue: 1; Related Information: CHORUS Timestamp: 2018-05-18 12:26:15; Journal ID: ISSN 1600-5767
Publisher:
International Union of Crystallography (IUCr)
Sponsoring Org:
USDOE
Country of Publication:
Denmark
Language:
English
OSTI Identifier:
1418182