Femtosecond X-ray diffraction from an aerosolized beam of protein nanocrystals

Awel, Salah; Kirian, Richard A.; Wiedorn, Max O.; Beyerlein, Kenneth R.; Roth, Nils; Horke, Daniel A.; Oberthür, Dominik; Knoska, Juraj; Mariani, Valerio; Morgan, Andrew; Adriano, Luigi; Tolstikova, Alexandra; Xavier, P. Lourdu; Yefanov, Oleksandr; Aquila, Andrew; Barty, Anton; Roy-Chowdhury, Shatabdi; Hunter, Mark S.; James, Daniel; Robinson, Joseph S.; Weierstall, Uwe; Rode, Andrei V.; Bajt, Saša; Küpper, Jochen; Chapman, Henry N.

doi:10.1107/s1600576717018131

Title: Femtosecond X-ray diffraction from an aerosolized beam of protein nanocrystals

Journal Article · 01 February 2018 · Journal of Applied Crystallography (Online)

DOI: https://doi.org/10.1107/s1600576717018131 · OSTI ID:1418182

^[1]; Kirian, Richard A. ^[2];

^[3]; Beyerlein, Kenneth R. ^[4]; Roth, Nils ^[4]; Horke, Daniel A. ^[1]; Oberthür, Dominik ^[4]; Knoska, Juraj ^[3]; Mariani, Valerio ^[4]; Morgan, Andrew ^[4]; Adriano, Luigi ^[5]; Tolstikova, Alexandra ^[3];

^[6]; Yefanov, Oleksandr ^[4]; Aquila, Andrew ^[7]; Barty, Anton ^[4]; Roy-Chowdhury, Shatabdi ^[2]; Hunter, Mark S. ^[7]; James, Daniel ^[2]; Robinson, Joseph S. ^[7] more »

Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany). Center for Free-Electron Laser Science; Univ. of Hamburg (Germany). Hamburg Center for Ultrafast Imaging
Arizona State Univ., Tempe, AZ (United States)
Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany). Center for Free-Electron Laser Science; Univ. of Hamburg (Germany). Dept. of Physics
Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany). Center for Free-Electron Laser Science
Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany). Photon Science
Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany). Center for Free-Electron Laser Science; Max-Planck Inst. for the Structure and Dynamics of Matter, Hamburg (Germany)
SLAC National Accelerator Lab., Menlo Park, CA (United States). Linac Coherent Light Source (LCLS)
Australian National Univ., Canberra, ACT (Australia). Laser Physics Centre, Research School of Physics and Engineering
Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany). Center for Free-Electron Laser Science; Univ. of Hamburg (Germany). Hamburg Center for Ultrafast Imaging; Univ. of Hamburg (Germany). Dept. of Physics

High-resolution Bragg diffraction from aerosolized single granulovirus nanocrystals using an X-ray free-electron laser is demonstrated. The outer dimensions of the in-vacuum aerosol injector components are identical to conventional liquid-microjet nozzles used in serial diffraction experiments, which allows the injector to be utilized with standard mountings. As compared with liquid-jet injection, the X-ray scattering background is reduced by several orders of magnitude by the use of helium carrier gas rather than liquid. Such reduction is required for diffraction measurements of small macromolecular nanocrystals and single particles. High particle speeds are achieved, making the approach suitable for use at upcoming high-repetition-rate facilities.

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Research Organization:: SLAC National Accelerator Laboratory (SLAC), Menlo Park, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES); National Institutes of Health (NIH); European Union (EU); National Science Foundation (NSF); German Research Foundation (DFG); USDOE

Grant/Contract Number:: AC02-76SF00515; P41GM103393; P41RR001209; ERC-Kupper-614507; 1231306

OSTI ID:: 1418182

Alternate ID(s):: OSTI ID: 1425348

Journal Information:: Journal of Applied Crystallography (Online), Vol. 51, Issue 1; ISSN 1600-5767

Publisher:: International Union of CrystallographyCopyright Statement

Country of Publication:: United States

Language:: English

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