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Title: Femtosecond X-ray diffraction from an aerosolized beam of protein nanocrystals

Journal Article · · Journal of Applied Crystallography (Online)
ORCiD logo [1];  [2]; ORCiD logo [3];  [4];  [4];  [1];  [4];  [3];  [4];  [4];  [5];  [3]; ORCiD logo [6];  [4];  [7];  [4];  [2];  [7];  [2];  [7] more »;  [2];  [8];  [5]; ORCiD logo [9]; ORCiD logo [9] « less
  1. Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany). Center for Free-Electron Laser Science; Univ. of Hamburg (Germany). Hamburg Center for Ultrafast Imaging
  2. Arizona State Univ., Tempe, AZ (United States)
  3. Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany). Center for Free-Electron Laser Science; Univ. of Hamburg (Germany). Dept. of Physics
  4. Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany). Center for Free-Electron Laser Science
  5. Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany). Photon Science
  6. Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany). Center for Free-Electron Laser Science; Max-Planck Inst. for the Structure and Dynamics of Matter, Hamburg (Germany)
  7. SLAC National Accelerator Lab., Menlo Park, CA (United States). Linac Coherent Light Source (LCLS)
  8. Australian National Univ., Canberra, ACT (Australia). Laser Physics Centre, Research School of Physics and Engineering
  9. Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany). Center for Free-Electron Laser Science; Univ. of Hamburg (Germany). Hamburg Center for Ultrafast Imaging; Univ. of Hamburg (Germany). Dept. of Physics

High-resolution Bragg diffraction from aerosolized single granulovirus nanocrystals using an X-ray free-electron laser is demonstrated. The outer dimensions of the in-vacuum aerosol injector components are identical to conventional liquid-microjet nozzles used in serial diffraction experiments, which allows the injector to be utilized with standard mountings. As compared with liquid-jet injection, the X-ray scattering background is reduced by several orders of magnitude by the use of helium carrier gas rather than liquid. Such reduction is required for diffraction measurements of small macromolecular nanocrystals and single particles. High particle speeds are achieved, making the approach suitable for use at upcoming high-repetition-rate facilities.

Research Organization:
SLAC National Accelerator Laboratory (SLAC), Menlo Park, CA (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Basic Energy Sciences (BES); National Institutes of Health (NIH); European Union (EU); National Science Foundation (NSF); German Research Foundation (DFG); USDOE
Grant/Contract Number:
AC02-76SF00515; P41GM103393; P41RR001209; ERC-Kupper-614507; 1231306
OSTI ID:
1418182
Alternate ID(s):
OSTI ID: 1425348
Journal Information:
Journal of Applied Crystallography (Online), Vol. 51, Issue 1; ISSN 1600-5767
Publisher:
International Union of CrystallographyCopyright Statement
Country of Publication:
United States
Language:
English

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Ultracompact 3D microfluidics for time-resolved structural biology journal January 2020
Plasma channel formation in NIR laser-irradiated carrier gas from an aerosol nanoparticle injector journal June 2019
Acoustic Funnel and Buncher for Nanoparticle Injection journal June 2019
Nanometre-sized droplets from a gas dynamic virtual nozzle journal July 2019
The Single Particles, Clusters and Biomolecules and Serial Femtosecond Crystallography instrument of the European XFEL: initial installation journal April 2019
A guide to sample delivery systems for serial crystallography journal August 2019
Acoustic Funnel and Buncher for Nanoparticle Injection text January 2019
Ultracompact 3D microfluidics for time-resolved structural biology text January 2020
Rayleigh-scattering microscopy for tracking and sizing nanoparticles in focused aerosol beams text January 2018
The Single Particles, Clusters and Biomolecules and Serial Femtosecond Crystallography instrument of the European XFEL: initial installation text January 2019
Ultracompact 3D microfluidics for time-resolved structural biology text January 2020

Figures / Tables (4)