Effective Labeling of Primary Somatic Stem Cells with BaTiO 3 Nanocrystals for Second Harmonic Generation Imaging

Sugiyama, Nami; Sonay, Ali Y.; Tussiwand, Roxanne; Cohen, Bruce E.; Pantazis, Periklis

doi:10.1002/smll.201703386

Title: Effective Labeling of Primary Somatic Stem Cells with BaTiO ₃ Nanocrystals for Second Harmonic Generation Imaging

Journal Article · 21 January 2018 · Small

DOI: https://doi.org/10.1002/smll.201703386 · OSTI ID:1417707

Sugiyama, Nami ^[1]; Sonay, Ali Y. ^[1]; Tussiwand, Roxanne ^[2]; Cohen, Bruce E. ^[3];

^[1]

Department of Biosystems Science and Engineering (D‐BSSE) Eidgenössische Technische Hochschule (ETH) Zurich 4058 Basel Switzerland
Department of Biomedicine University of Basel 4058 Basel Switzerland
The Molecular Foundry Lawrence Berkeley National Laboratory Berkeley CA 94720 USA

Abstract While nanoparticles are an increasingly popular choice for labeling and tracking stem cells in biomedical applications such as cell therapy, their intracellular fate and subsequent effect on stem cell differentiation remain elusive. To establish an effective stem cell labeling strategy, the intracellular nanocrystal concentration should be minimized to avoid adverse effects, without compromising the intensity and persistence of the signal necessary for long‐term tracking. Here, the use of second‐harmonic generating barium titanate nanocrystals is reported, whose achievable brightness allows for high contrast stem cell labeling with at least one order of magnitude lower intracellular nanocrystals than previously reported. Their long‐term photostability enables to investigate quantitatively at the single cell level their cellular fate in hematopoietic stem cells (HSCs) using both multiphoton and electron microscopy. It is found that the concentration of nanocrystals in proliferative multipotent progenitors is over 2.5‐fold greater compared to quiescent stem cells; this difference vanishes when HSCs enter a nonquiescent, proliferative state, while their potency remains unaffected. Understanding the nanoparticle stem cell interaction allows to establish an effective and safe nanoparticle labeling strategy into somatic stem cells that can critically contribute to an understanding of their in vivo therapeutic potential.

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Sponsoring Organization:: USDOE

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1417707

Journal Information:: Small, Journal Name: Small Journal Issue: 8 Vol. 14; ISSN 1613-6810

Publisher:: Wiley Blackwell (John Wiley & Sons)Copyright Statement

Country of Publication:: Germany

Language:: English

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Title: Effective Labeling of Primary Somatic Stem Cells with BaTiO ₃ Nanocrystals for Second Harmonic Generation Imaging