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Title: Multistage bioassociation of uranium onto an extremely halophilic archaeon revealed by a unique combination of spectroscopic and microscopic techniques

The interactions of two extremely halophilic archaea with uranium were investigated in this paper at high ionic strength as a function of time, pH and uranium concentration. Halobacterium noricense DSM-15987 and Halobacterium sp. putatively noricense, isolated from the Waste Isolation Pilot Plant repository, were used for these investigations. The kinetics of U(VI) bioassociation with both strains showed an atypical multistage behavior, meaning that after an initial phase of U(VI) sorption, an unexpected interim period of U(VI) release was observed, followed by a slow reassociation of uranium with the cells. By applying in situ attenuated total reflection Fourier-transform infrared spectroscopy, the involvement of phosphoryl and carboxylate groups in U(VI) complexation during the first biosorption phase was shown. Differences in cell morphology and uranium localization become visible at different stages of the bioassociation process, as shown with scanning electron microscopy in combination with energy dispersive X-ray spectroscopy. Finally, our results demonstrate for the first time that association of uranium with the extremely halophilic archaeon is a multistage process, beginning with sorption and followed by another process, probably biomineralization.
Authors:
 [1] ;  [1] ;  [1] ;  [1] ;  [2] ;  [2] ;  [3] ;  [3] ;  [1] ;  [1]
  1. Helmholtz-Zentrum Dresden-Rossendorf (HZDR), Dresden (Germany). Inst. of Resource Ecology
  2. Helmholtz Centre for Environmental Research - UFZ, Leipzig (Germany). Dept. of Isotope Biogeochemistry
  3. Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
Publication Date:
Report Number(s):
LA-UR-17-27411
Journal ID: ISSN 0304-3894; TRN: US1800904
Grant/Contract Number:
AC52-06NA25396; 02E10971
Type:
Accepted Manuscript
Journal Name:
Journal of Hazardous Materials
Additional Journal Information:
Journal Volume: 327; Journal ID: ISSN 0304-3894
Publisher:
Elsevier
Research Org:
Helmholtz-Zentrum Dresden-Rossendorf (HZDR), Dresden (Germany); Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
Sponsoring Org:
USDOE; Federal Ministry for Economic Affairs and Energy (BMWi) (Germany)
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; 58 GEOSCIENCES; uranium bioassociation; halophilic archaeon Halobacterium noricense; in situ ATR FT-IR spectroscopy; luminescence spectroscopy at high salinity; final disposal of radioactive waste
OSTI Identifier:
1416296