Linking secondary metabolites to gene clusters through genome sequencing of six diverse Aspergillus species

Kjerbolling, Inge; Vesth, Tammi C.; Frisvad, Jens C.; Nybo, Jane L.; Theobald, Sebastian; Kuo, Alan; Bowyer, Paul; Matsuda, Yudai; Mondo, Stephen; Lyhne, Ellen K.; Kogle, Martin E.; Clum, Alicia; Lipzen, Anna; Salamov, Asaf; Ngan, Chew Yee; Daum, Chris; Chiniquy, Jennifer; Barry, Kerrie; LaButti, Kurt; Haridas, Sajeet; Simmons, Blake A.; Magnuson, Jon K.; Mortensen, Uffe H.; Larsen, Thomas O.; Grigoriev, Igor V.; Baker, Scott E.; Andersen, Mikael R.

doi:10.1073/pnas.1715954115

Title: Linking secondary metabolites to gene clusters through genome sequencing of six diverse Aspergillus species

Journal Article · 09 January 2018 · Proceedings of the National Academy of Sciences of the United States of America

DOI: https://doi.org/10.1073/pnas.1715954115 · OSTI ID:1416221

Kjerbolling, Inge ^[1]; Vesth, Tammi C. ^[1]; Frisvad, Jens C. ^[1]; Nybo, Jane L. ^[1]; Theobald, Sebastian ^[1]; Kuo, Alan ^[2]; Bowyer, Paul ^[3]; Matsuda, Yudai ^[1]; Mondo, Stephen ^[2]; Lyhne, Ellen K. ^[1]; Kogle, Martin E. ^[1]; Clum, Alicia ^[2]; Lipzen, Anna ^[2]; Salamov, Asaf ^[2]; Ngan, Chew Yee ^[2]; Daum, Chris ^[2]; Chiniquy, Jennifer ^[2]; Barry, Kerrie ^[2]; LaButti, Kurt ^[2]; Haridas, Sajeet ^[2] more »

Technical Univ. of Denmark, Lyngby (Denmark)
USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States)
Univ. of Manchester (United Kingdom)
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Joint BioEnergy Inst. (JBEI), Emeryville, CA (United States)
Joint BioEnergy Inst. (JBEI), Emeryville, CA (United States); Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States); Univ. of California, Berkeley, CA (United States)
USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States); Pacific Northwest National Lab. (PNNL), Richland, WA (United States)

The fungal genus of Aspergillus is highly interesting, containing everything from industrial cell factories over model organisms to human pathogens. In particular, this group has a prolific production of bioactive secondary metabolites (SMs). In this work, four diverse Aspergillus species (A. campestris, A. novofumigatus, A. ochraceoroseus and A. steynii) has been whole genome PacBio sequenced to provide genetic references in three Aspergillus sections. Additionally, A. taichungensis and A. candidus were sequenced for SM elucidation. Thirteen Aspergillus genomes were analysed with comparative genomics to determine phylogeny and genetic diversity, showing that each new genome contains 15–27% genes not found in other sequenced Aspergilli. In particular, the new species A. novofumigatus was compared to the pathogenic species A. fumigatus. This suggests that A. novofumigatus can produce most of the same allergens, virulence and pathogenicity factors as A. fumigatus suggesting that A. novofumigatus could be as pathogenic as A. fumigatus. Furthermore, SMs were linked to gene clusters based on biological and chemical knowledge and analysis, genome sequences and predictive algorithms.

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Research Organization:: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States); Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC05-76RL01830; AC02-05CH11231

OSTI ID:: 1416221

Alternate ID(s):: OSTI ID: 1439668; OSTI ID: 1465428

Report Number(s):: PNNL-SA-131168; 830403000

Journal Information:: Proceedings of the National Academy of Sciences of the United States of America, Vol. 115, Issue 4; ISSN 0027-8424

Publisher:: National Academy of Sciences, Washington, DC (United States)Copyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 78 works

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