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Title: Analysis of XFEL serial diffraction data from individual crystalline fibrils

Serial diffraction data collected at the Linac Coherent Light Source from crystalline amyloid fibrils delivered in a liquid jet show that the fibrils are well oriented in the jet. At low fibril concentrations, diffraction patterns are recorded from single fibrils; these patterns are weak and contain only a few reflections. Methods are developed for determining the orientation of patterns in reciprocal space and merging them in three dimensions. This allows the individual structure amplitudes to be calculated, thus overcoming the limitations of orientation and cylindrical averaging in conventional fibre diffraction analysis. In conclusion, the advantages of this technique should allow structural studies of fibrous systems in biology that are inaccessible using existing techniques.
 [1] ;  [2] ;  [3] ;  [4] ;  [5] ;  [2] ;  [2] ;  [6] ;  [2] ;  [2] ;  [2] ;  [7] ;  [2] ;  [2] ;  [2] ;  [8] ;  [2] ;  [9] ;  [10] ;  [11] more »;  [3] ;  [2] ;  [2] ;  [8] ;  [3] ;  [3] ;  [2] ;  [4] ;  [12] ;  [1] « less
  1. Univ. of Canterbury, Christchurch (New Zealand)
  2. Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany)
  3. SLAC National Accelerator Lab., Menlo Park, CA (United States)
  4. Institut Laue-Langevin, Grenoble (France); Keele Univ. (England)
  5. European Synchrotron Radiation Facility, Grenoble (France)
  6. European XFEL GmbH, Hamburg (Germany)
  7. Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany); Max Planck Institute of Biochemistry, Martinsried (Germany)
  8. Univ. of Crete and IESL/FORTH, Crete (Greece)
  9. Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany); Max-Planck Institute for the Structure and Dynamics of Matter, Hamburg (Germany)
  10. Univ. Grenoble Alpes, Grenoble (France)
  11. Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
  12. Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany); Univ. of Hamburg, Hamburg (Germany)
Publication Date:
Grant/Contract Number:
AC52-07NA27344; 1R01GM117342-01; AC02- 76SF00515
Published Article
Journal Name:
Additional Journal Information:
Journal Volume: 4; Journal Issue: 6; Journal ID: ISSN 2052-2525
International Union of Crystallography
Research Org:
SLAC National Accelerator Lab., Menlo Park, CA (United States)
Sponsoring Org:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
Country of Publication:
United States
36 MATERIALS SCIENCE; 72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS; serial crystallography; coherent X-ray diffractive imaging (CXDI); single particles; molecular orientation determination; crystalline fibrils; amyloid
OSTI Identifier:
Alternate Identifier(s):
OSTI ID: 1410555