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Title: Analysis of XFEL serial diffraction data from individual crystalline fibrils

Abstract

Serial diffraction data collected at the Linac Coherent Light Source from crystalline amyloid fibrils delivered in a liquid jet show that the fibrils are well oriented in the jet. At low fibril concentrations, diffraction patterns are recorded from single fibrils; these patterns are weak and contain only a few reflections. Methods are developed for determining the orientation of patterns in reciprocal space and merging them in three dimensions. This allows the individual structure amplitudes to be calculated, thus overcoming the limitations of orientation and cylindrical averaging in conventional fibre diffraction analysis. The advantages of this technique should allow structural studies of fibrous systems in biology that are inaccessible using existing techniques.

Authors:
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Publication Date:
Research Org.:
SLAC National Accelerator Laboratory (SLAC), Menlo Park, CA (United States); Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES); USDOE National Nuclear Security Administration (NNSA); National Institutes of Health (NIH)
OSTI Identifier:
1416058
Alternate Identifier(s):
OSTI ID: 1410555; OSTI ID: 2203629
Report Number(s):
LLNL-JRNL-856473
Journal ID: ISSN 2052-2525; IUCRAJ; PII: S2052252517014324
Grant/Contract Number:  
AC52-07NA27344; AC02- 76SF00515; 1R01GM117342-01; AC02-76SF00515
Resource Type:
Published Article
Journal Name:
IUCrJ
Additional Journal Information:
Journal Name: IUCrJ Journal Volume: 4 Journal Issue: 6; Journal ID: ISSN 2052-2525
Publisher:
International Union of Crystallography (IUCr)
Country of Publication:
United Kingdom
Language:
English
Subject:
36 MATERIALS SCIENCE; 72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS; serial crystallography; coherent X-ray diffractive imaging (CXDI); single particles; molecular orientation determination; crystalline fibrils; amyloid

Citation Formats

Wojtas, David H., Ayyer, Kartik, Liang, Mengning, Mossou, Estelle, Romoli, Filippo, Seuring, Carolin, Beyerlein, Kenneth R., Bean, Richard J., Morgan, Andrew J., Oberthuer, Dominik, Fleckenstein, Holger, Heymann, Michael, Gati, Cornelius, Yefanov, Oleksandr, Barthelmess, Miriam, Ornithopoulou, Eirini, Galli, Lorenzo, Xavier, P. Lourdu, Ling, Wai Li, Frank, Matthias, Yoon, Chun Hong, White, Thomas A., Bajt, Saša, Mitraki, Anna, Boutet, Sebastien, Aquila, Andrew, Barty, Anton, Forsyth, V. Trevor, Chapman, Henry N., and Millane, Rick P. Analysis of XFEL serial diffraction data from individual crystalline fibrils. United Kingdom: N. p., 2017. Web. doi:10.1107/S2052252517014324.
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Wojtas, David H., Ayyer, Kartik, Liang, Mengning, Mossou, Estelle, Romoli, Filippo, Seuring, Carolin, Beyerlein, Kenneth R., Bean, Richard J., Morgan, Andrew J., Oberthuer, Dominik, Fleckenstein, Holger, Heymann, Michael, Gati, Cornelius, Yefanov, Oleksandr, Barthelmess, Miriam, Ornithopoulou, Eirini, Galli, Lorenzo, Xavier, P. Lourdu, Ling, Wai Li, Frank, Matthias, Yoon, Chun Hong, White, Thomas A., Bajt, Saša, Mitraki, Anna, Boutet, Sebastien, Aquila, Andrew, Barty, Anton, Forsyth, V. Trevor, Chapman, Henry N., & Millane, Rick P. Analysis of XFEL serial diffraction data from individual crystalline fibrils. United Kingdom. https://doi.org/10.1107/S2052252517014324
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Wojtas, David H., Ayyer, Kartik, Liang, Mengning, Mossou, Estelle, Romoli, Filippo, Seuring, Carolin, Beyerlein, Kenneth R., Bean, Richard J., Morgan, Andrew J., Oberthuer, Dominik, Fleckenstein, Holger, Heymann, Michael, Gati, Cornelius, Yefanov, Oleksandr, Barthelmess, Miriam, Ornithopoulou, Eirini, Galli, Lorenzo, Xavier, P. Lourdu, Ling, Wai Li, Frank, Matthias, Yoon, Chun Hong, White, Thomas A., Bajt, Saša, Mitraki, Anna, Boutet, Sebastien, Aquila, Andrew, Barty, Anton, Forsyth, V. Trevor, Chapman, Henry N., and Millane, Rick P. Fri . "Analysis of XFEL serial diffraction data from individual crystalline fibrils". United Kingdom. https://doi.org/10.1107/S2052252517014324.
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@article{osti_1416058,
title = {Analysis of XFEL serial diffraction data from individual crystalline fibrils},
author = {Wojtas, David H. and Ayyer, Kartik and Liang, Mengning and Mossou, Estelle and Romoli, Filippo and Seuring, Carolin and Beyerlein, Kenneth R. and Bean, Richard J. and Morgan, Andrew J. and Oberthuer, Dominik and Fleckenstein, Holger and Heymann, Michael and Gati, Cornelius and Yefanov, Oleksandr and Barthelmess, Miriam and Ornithopoulou, Eirini and Galli, Lorenzo and Xavier, P. Lourdu and Ling, Wai Li and Frank, Matthias and Yoon, Chun Hong and White, Thomas A. and Bajt, Saša and Mitraki, Anna and Boutet, Sebastien and Aquila, Andrew and Barty, Anton and Forsyth, V. Trevor and Chapman, Henry N. and Millane, Rick P.},
abstractNote = {Serial diffraction data collected at the Linac Coherent Light Source from crystalline amyloid fibrils delivered in a liquid jet show that the fibrils are well oriented in the jet. At low fibril concentrations, diffraction patterns are recorded from single fibrils; these patterns are weak and contain only a few reflections. Methods are developed for determining the orientation of patterns in reciprocal space and merging them in three dimensions. This allows the individual structure amplitudes to be calculated, thus overcoming the limitations of orientation and cylindrical averaging in conventional fibre diffraction analysis. The advantages of this technique should allow structural studies of fibrous systems in biology that are inaccessible using existing techniques.},
doi = {10.1107/S2052252517014324},
journal = {IUCrJ},
number = 6,
volume = 4,
place = {United Kingdom},
year = {2017},
month = {10}
}
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https://doi.org/10.1107/S2052252517014324
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Cited by: 16 works
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Figures / Tables:

Figure 1 Figure 1: Electron micrograph of the adenovirus amyloid fibrils.
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    Works referencing / citing this record:

    Coherent diffractive imaging of microtubules using an X-ray laser
    journal, June 2019

    Analysis of XFEL serial diffraction data from individual crystalline fibrils
    text, January 2017

    • Wojtas, David H.; Ayyer, Kartik; Liang, Mengning
    • Deutsches Elektronen-Synchrotron, DESY, Hamburg
    • DOI: 10.3204/pubdb-2017-11523

    Ultracompact 3D microfluidics for time-resolved structural biology
    journal, January 2020

    Strategies for sample delivery for femtosecond crystallography
    journal, February 2019

    • Martiel, Isabelle; Müller-Werkmeister, Henrike M.; Cohen, Aina E.
    • Acta Crystallographica Section D Structural Biology, Vol. 75, Issue 2
    • DOI: 10.1107/s2059798318017953

    Femtosecond X-ray coherent diffraction of aligned amyloid fibrils on low background graphene
    text, January 2018

    • Seuring, Carolin; Ayyer, Kartik; Filippaki, Eleftheria
    • Deutsches Elektronen-Synchrotron, DESY, Hamburg
    • DOI: 10.3204/pubdb-2018-01731

    Coherent diffractive imaging of microtubules using an X-ray laser
    text, January 2019

    • Brändén, Gisela; Hammarin, Greger; Harimoorthy, Rajiv
    • Deutsches Elektronen-Synchrotron, DESY, Hamburg
    • DOI: 10.3204/pubdb-2019-03073
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      Figures / Tables found in this record:

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      Table 1 (p. 17)table
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