In Vitro Characterization and Concerted Function of Three Core Enzymes of a Glycyl Radical Enzyme - Associated Bacterial Microcompartment

Zarzycki, Jan; Sutter, Markus; Cortina, Niña Socorro; Erb, Tobias J.; Kerfeld, Cheryl A.

doi:10.1038/srep42757

Title: In Vitro Characterization and Concerted Function of Three Core Enzymes of a Glycyl Radical Enzyme - Associated Bacterial Microcompartment

Journal Article · 16 February 2017 · Scientific Reports

DOI: https://doi.org/10.1038/srep42757 · OSTI ID:1415968

Zarzycki, Jan ^[1]; Sutter, Markus ^[2]; Cortina, Niña Socorro ^[1]; Erb, Tobias J. ^[1]; Kerfeld, Cheryl A. ^[3]

Max Planck Inst. for Terrestrial Microbiology, Marburg (Germany)
Michigan State Univ., East Lansing, MI (United States). MSU-DOE Plant Research Lab.; Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Michigan State Univ., East Lansing, MI (United States). MSU-DOE Plant Research Lab., Dept. of Biochemistry and Molecular Biology; Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Berkeley Synthetic Biology Inst., Berkeley, CA (United States); Univ. of California, Berkeley, CA (United States). Dept. of Plant and Microbial Biology

Many bacteria encode proteinaceous bacterial microcompartments (BMCs) that encapsulate sequential enzymatic reactions of diverse metabolic pathways. Well-characterized BMCs include carboxysomes for CO₂-fixation, and propanediol- and ethanolamine-utilizing microcompartments that contain B₁₂-dependent enzymes. Genes thus required to form BMCs are typically organized in gene clusters, which promoted their distribution across phyla by horizontal gene transfer. Recently, BMCs associated with glycyl radical enzymes (GREs) were discovered; these are widespread and comprise at least three functionally distinct types. Previously, we predicted one type of these GRE-associated microcompartments (GRMs) represents a B₁₂-independent propanediol-utilizing BMC. We functionally and structurally characterize enzymes of the GRM of Rhodopseudomonas palustris BisB18 and demonstrate their concerted function in vitro. The GRM signature enzyme, the GRE, is a dedicated 1,2-propanediol dehydratase with a new type of intramolecular encapsulation peptide. It forms a complex with its activating enzyme and, in conjunction with an aldehyde dehydrogenase, converts 1,2-propanediol to propionyl-CoA. Notably, homologous GRMs are also encoded in pathogenic Escherichia coli strains. Our high-resolution crystal structures of the aldehyde dehydrogenase lead to a revised reaction mechanism. The successful in vitro reconstitution of a part of the GRM metabolism provides insights into the metabolic function and steps in the assembly of this BMC.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1415968

Journal Information:: Scientific Reports, Vol. 7; ISSN 2045-2322

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 36 works

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References (68)

Bacterial microcompartments and the modular construction of microbial metabolism Kerfeld, Cheryl A.; Erbilgin, Onur Trends in Microbiology, Vol. 23, Issue 1 https://doi.org/10.1016/j.tim.2014.10.003	journal	January 2015
Bacterial microcompartments and the modular construction of microbial metabolism Kerfeld, Cheryl A.; Erbilgin, Onur Trends in Microbiology, Vol. 23, Issue 1 https://doi.org/10.1016/j.tim.2014.10.003	journal	January 2015
Interactions between the termini of lumen enzymes and shell proteins mediate enzyme encapsulation into bacterial microcompartments Fan, C.; Cheng, S.; Sinha, S. Proceedings of the National Academy of Sciences, Vol. 109, Issue 37 https://doi.org/10.1073/pnas.1207516109	journal	August 2012
PHENIX: a comprehensive Python-based system for macromolecular structure solution. Adams, Paul D.; Afonine, Pavel V.; Bunkóczi, Gábor Apollo - University of Cambridge Repository https://doi.org/10.17863/cam.45787	text	January 2010
Substrate Specificity, Substrate Channeling, and Allostery in BphJ: An Acylating Aldehyde Dehydrogenase Associated with the Pyruvate Aldolase BphI Baker, Perrin; Carere, Jason; Seah, Stephen Y. K. Biochemistry, Vol. 51, Issue 22 https://doi.org/10.1021/bi300407y	journal	May 2012
Substrate Specificity, Substrate Channeling, and Allostery in BphJ: An Acylating Aldehyde Dehydrogenase Associated with the Pyruvate Aldolase BphI Baker, Perrin; Carere, Jason; Seah, Stephen Y. K. Biochemistry, Vol. 51, Issue 22 https://doi.org/10.1021/bi300407y	journal	May 2012
Involvement of a Bacterial Microcompartment in the Metabolism of Fucose and Rhamnose by Clostridium phytofermentans Petit, Elsa; LaTouf, W. Greg; Coppi, Maddalena V. PLoS ONE, Vol. 8, Issue 1 https://doi.org/10.1371/journal.pone.0054337	journal	January 2013
Employing bacterial microcompartment technology to engineer a shell-free enzyme-aggregate for enhanced 1,2-propanediol production in Escherichia coli Lee, Matthew J.; Brown, Ian R.; Juodeikis, Rokas Metabolic Engineering, Vol. 36 https://doi.org/10.1016/j.ymben.2016.02.007	journal	July 2016
The 17-Gene Ethanolamine (eut) Operon ofSalmonella typhimurium Encodes Five Homologues of Carboxysome Shell Proteins Kofoid, Eric; Rappleye, Chad; Stojiljkovic, Igor Journal of Bacteriology, Vol. 181, Issue 17 https://doi.org/10.1128/JB.181.17.5317-5329.1999	journal	September 1999
The Propanediol Utilization (pdu) Operon ofSalmonella enterica Serovar Typhimurium LT2 Includes Genes Necessary for Formation of Polyhedral Organelles Involved in Coenzyme B12-Dependent 1,2-Propanediol Degradation † Bobik, Thomas A.; Havemann, Gregory D.; Busch, Robert J. Journal of Bacteriology, Vol. 181, Issue 19 https://doi.org/10.1128/JB.181.19.5967-5975.1999	journal	January 1999
PduP is a coenzyme-a-acylating propionaldehyde dehydrogenase associated with the polyhedral bodies involved in B 12 -dependent 1,2-propanediol degradation by Salmonella enterica serovar Typhimurium LT2 Leal, Nicole A.; Havemann, Gregory D.; Bobik, Thomas A. Archives of Microbiology, Vol. 180, Issue 5 https://doi.org/10.1007/s00203-003-0601-0	journal	November 2003
Interactions between the termini of lumen enzymes and shell proteins mediate enzyme encapsulation into bacterial microcompartments Fan, C.; Cheng, S.; Sinha, S. Proceedings of the National Academy of Sciences, Vol. 109, Issue 37 https://doi.org/10.1073/pnas.1207516109	journal	August 2012
Converging on a mechanism for choline degradation Thibodeaux, C. J.; van der Donk, W. A. Proceedings of the National Academy of Sciences, Vol. 109, Issue 52 https://doi.org/10.1073/pnas.1219534110	journal	December 2012
Radical SAM Activation of the B ₁₂ -Independent Glycerol Dehydratase Results in Formation of 5′-Deoxy-5′-(methylthio)adenosine and Not 5′-Deoxyadenosine Demick, Jonathan M.; Lanzilotta, William N. Biochemistry, Vol. 50, Issue 4 https://doi.org/10.1021/bi101255e	journal	February 2011
A Taxonomy of Bacterial Microcompartment Loci Constructed by a Novel Scoring Method Axen, Seth D.; Erbilgin, Onur; Kerfeld, Cheryl A. PLoS Computational Biology, Vol. 10, Issue 10 https://doi.org/10.1371/journal.pcbi.1003898	journal	October 2014
Coenzyme A-acylating aldehyde dehydrogenase from Clostridium beijerinckii NRRL B592. Yan, R. T.; Chen, J. S. Applied and Environmental Microbiology, Vol. 56, Issue 9 https://doi.org/10.1128/aem.56.9.2591-2599.1990	journal	January 1990
A Taxonomy of Bacterial Microcompartment Loci Constructed by a Novel Scoring Method Axen, Seth D.; Erbilgin, Onur; Kerfeld, Cheryl A. PLoS Computational Biology, Vol. 10, Issue 10 https://doi.org/10.1371/journal.pcbi.1003898	journal	October 2014
Dumpster Diving in the Gut: Bacterial Microcompartments as Part of a Host-Associated Lifestyle Jakobson, Christopher M.; Tullman-Ercek, Danielle PLOS Pathogens, Vol. 12, Issue 5 https://doi.org/10.1371/journal.ppat.1005558	journal	May 2016
Steady-state and pre-steady-state kinetics of coenzyme A-linked aldehyde dehydrogenase from Escherichia coli Shone, Clifford C.; Fromm, Herbert J. Biochemistry, Vol. 20, Issue 26 https://doi.org/10.1021/bi00529a026	journal	December 1981
Identification and characterization of the propanediol utilization protein PduP of Lactobacillus reuteri for 3-hydroxypropionic acid production from glycerol Luo, Lian Hua; Seo, Jeong-Woo; Baek, Jin-Oh Applied Microbiology and Biotechnology, Vol. 89, Issue 3 https://doi.org/10.1007/s00253-010-2887-6	journal	October 2010
Bacterial microcompartments moving into a synthetic biological world Frank, Stefanie; Lawrence, Andrew D.; Prentice, Michael B. Journal of Biotechnology, Vol. 163, Issue 2 https://doi.org/10.1016/j.jbiotec.2012.09.002	journal	January 2013
How to become a uropathogen: Comparative genomic analysis of extraintestinal pathogenic Escherichia coli strains Brzuszkiewicz, E.; Bruggemann, H.; Liesegang, H. Proceedings of the National Academy of Sciences, Vol. 103, Issue 34 https://doi.org/10.1073/pnas.0603038103	journal	August 2006
XDS Kabsch, Wolfgang Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 2 https://doi.org/10.1107/S0907444909047337	journal	January 2010
Molecular Basis of C–N Bond Cleavage by the Glycyl Radical Enzyme Choline Trimethylamine-Lyase Bodea, Smaranda; Funk, Michael A.; Balskus, Emily P. Cell Chemical Biology, Vol. 23, Issue 10 https://doi.org/10.1016/j.chembiol.2016.07.020	journal	October 2016
Investigation of the Active Site Cysteine Residue of Rat Liver Mitochondrial Aldehyde Dehydrogenase by Site-Directed Mutagenesis Farres, Jaume; Wang, Thomas T. Y.; Cunningham, Suzanne J. Biochemistry, Vol. 34, Issue 8 https://doi.org/10.1021/bi00008a025	journal	February 1995
Elucidating Essential Role of Conserved Carboxysomal Protein CcmN Reveals Common Feature of Bacterial Microcompartment Assembly Kinney, James N.; Salmeen, Annette; Cai, Fei Journal of Biological Chemistry, Vol. 287, Issue 21 https://doi.org/10.1074/jbc.M112.355305	journal	March 2012
Microbial conversion of choline to trimethylamine requires a glycyl radical enzyme Craciun, S.; Balskus, E. P. Proceedings of the National Academy of Sciences, Vol. 109, Issue 52 https://doi.org/10.1073/pnas.1215689109	journal	November 2012
Solution Structure of a Bacterial Microcompartment Targeting Peptide and Its Application in the Construction of an Ethanol Bioreactor Lawrence, Andrew D.; Frank, Stefanie; Newnham, Sarah ACS Synthetic Biology, Vol. 3, Issue 7 https://doi.org/10.1021/sb4001118	journal	November 2013
Biogenesis of a Bacterial Organelle: The Carboxysome Assembly Pathway Cameron, Jeffrey C.; Wilson, Steven C.; Bernstein, Susan L. Cell, Vol. 155, Issue 5 https://doi.org/10.1016/j.cell.2013.10.044	journal	November 2013
Biogenesis of a Bacterial Organelle: The Carboxysome Assembly Pathway Cameron, Jeffrey C.; Wilson, Steven C.; Bernstein, Susan L. Cell, Vol. 155, Issue 5 https://doi.org/10.1016/j.cell.2013.10.044	journal	November 2013
Purification, properties, and kinetic mechanism of coenzyme A-linked aldehyde dehydrogenase from Clostridium kluyveri Smith, Linda Tombras; Kaplan, Nathan O. Archives of Biochemistry and Biophysics, Vol. 203, Issue 2 https://doi.org/10.1016/0003-9861(80)90224-6	journal	September 1980
The N-Terminal Regions of β and γ Subunits Lower the Solubility of Adenosylcobalamin-Dependent Diol Dehydratase Tobimatsu, Takamasa; Kawata, Masahiro; Toraya, Tetsuo Bioscience, Biotechnology, and Biochemistry, Vol. 69, Issue 3 https://doi.org/10.1271/bbb.69.455	journal	January 2005
Purification, properties, and kinetic mechanism of coenzyme A-linked aldehyde dehydrogenase from Clostridium kluyveri Smith, Linda Tombras; Kaplan, Nathan O. Archives of Biochemistry and Biophysics, Vol. 203, Issue 2 https://doi.org/10.1016/0003-9861(80)90224-6	journal	September 1980
The N-Terminal Regions of β and γ Subunits Lower the Solubility of Adenosylcobalamin-Dependent Diol Dehydratase Tobimatsu, Takamasa; Kawata, Masahiro; Toraya, Tetsuo Bioscience, Biotechnology, and Biochemistry, Vol. 69, Issue 3 https://doi.org/10.1271/bbb.69.455	journal	January 2005
Insight into Coenzyme A cofactor binding and the mechanism of acyl-transfer in an acylating aldehyde dehydrogenase from Clostridium phytofermentans Tuck, Laura R.; Altenbach, Kirsten; Ang, Thiau Fu Scientific Reports, Vol. 6, Issue 1 https://doi.org/10.1038/srep22108	journal	February 2016
A Chemo-Enzymatic Road Map to the Synthesis of CoA Esters Peter, Dominik; Vögeli, Bastian; Cortina, Niña Molecules, Vol. 21, Issue 4 https://doi.org/10.3390/molecules21040517	journal	April 2016
Bacterial microcompartment assembly: The key role of encapsulation peptides Aussignargues, Clément; Paasch, Bradley C.; Gonzalez-Esquer, Raul Communicative & Integrative Biology, Vol. 8, Issue 3 https://doi.org/10.1080/19420889.2015.1039755	journal	May 2015
Bacterial microcompartments moving into a synthetic biological world Frank, Stefanie; Lawrence, Andrew D.; Prentice, Michael B. Journal of Biotechnology, Vol. 163, Issue 2 https://doi.org/10.1016/j.jbiotec.2012.09.002	journal	January 2013
Coot model-building tools for molecular graphics Emsley, Paul; Cowtan, Kevin Acta Crystallographica Section D Biological Crystallography, Vol. 60, Issue 12, p. 2126-2132 https://doi.org/10.1107/S0907444904019158	journal	November 2004
Structure and Function of CutC Choline Lyase from Human Microbiota Bacterium Klebsiella pneumoniae Kalnins, Gints; Kuka, Janis; Grinberga, Solveiga Journal of Biological Chemistry, Vol. 290, Issue 35 https://doi.org/10.1074/jbc.M115.670471	journal	July 2015
Bacterial microcompartments as metabolic modules for plant synthetic biology Gonzalez-Esquer, C. Raul; Newnham, Sarah E.; Kerfeld, Cheryl A. The Plant Journal, Vol. 87, Issue 1 https://doi.org/10.1111/tpj.13166	journal	June 2016
Proteins Containing 4Fe-4S Clusters: An Overview Sweeney, W. V.; Rabinowitz, J. C. Annual Review of Biochemistry, Vol. 49, Issue 1 https://doi.org/10.1146/annurev.bi.49.070180.001035	journal	June 1980
1,2-Propanediol Dehydration in Roseburia inulinivorans : STRUCTURAL BASIS FOR SUBSTRATE AND ENANTIOMER SELECTIVITY LaMattina, Joseph W.; Keul, Nicholas D.; Reitzer, Pierre Journal of Biological Chemistry, Vol. 291, Issue 30 https://doi.org/10.1074/jbc.M116.721142	journal	June 2016
Molecular characterization of the 1,3-propanediol (1,3-PD) operon of Clostridium butyricum Raynaud, C.; Sarcabal, P.; Meynial-Salles, I. Proceedings of the National Academy of Sciences, Vol. 100, Issue 9 https://doi.org/10.1073/pnas.0734105100	journal	April 2003
Dumpster Diving in the Gut: Bacterial Microcompartments as Part of a Host-Associated Lifestyle Jakobson, Christopher M.; Tullman-Ercek, Danielle PLOS Pathogens, Vol. 12, Issue 5 https://doi.org/10.1371/journal.ppat.1005558	journal	May 2016
Correction to Characterization of Choline Trimethylamine-Lyase Expands the Chemistry of Glycyl Radical Enzymes Craciun, Smaranda; Marks, Jonathan A.; Balskus, Emily P. ACS Chemical Biology, Vol. 11, Issue 7 https://doi.org/10.1021/acschembio.6b00487	journal	June 2016
How to become a uropathogen: Comparative genomic analysis of extraintestinal pathogenic Escherichia coli strains Brzuszkiewicz, E.; Bruggemann, H.; Liesegang, H. Proceedings of the National Academy of Sciences, Vol. 103, Issue 34 https://doi.org/10.1073/pnas.0603038103	journal	August 2006
Characterization of Choline Trimethylamine-Lyase Expands the Chemistry of Glycyl Radical Enzymes Craciun, Smaranda; Marks, Jonathan A.; Balskus, Emily P. ACS Chemical Biology, Vol. 9, Issue 7 https://doi.org/10.1021/cb500113p	journal	May 2014
PHENIX: a comprehensive Python-based system for macromolecular structure solution Adams, Paul D.; Afonine, Pavel V.; Bunkóczi, Gábor Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 2, p. 213-221 https://doi.org/10.1107/S0907444909052925	journal	January 2010
Bioinformatic Characterization of Glycyl Radical Enzyme-Associated Bacterial Microcompartments Zarzycki, Jan; Erbilgin, Onur; Kerfeld, Cheryl A. Applied and Environmental Microbiology, Vol. 81, Issue 24 https://doi.org/10.1128/AEM.02587-15	journal	September 2015
Structural and biochemical investigations of the catalytic mechanism of an NADP-dependent aldehyde dehydrogenase from Streptococcus mutans Cobessi, David; Tête-Favier, Frédérique; Marchal, Stéphane Journal of Molecular Biology, Vol. 300, Issue 1 https://doi.org/10.1006/jmbi.2000.3824	journal	June 2000
Solution Structure of a Bacterial Microcompartment Targeting Peptide and Its Application in the Construction of an Ethanol Bioreactor Lawrence, Andrew D.; Frank, Stefanie; Newnham, Sarah ACS Synthetic Biology, Vol. 3, Issue 7 https://doi.org/10.1021/sb4001118	journal	November 2013
Identification and characterization of the propanediol utilization protein PduP of Lactobacillus reuteri for 3-hydroxypropionic acid production from glycerol Luo, Lian Hua; Seo, Jeong-Woo; Baek, Jin-Oh Applied Microbiology and Biotechnology, Vol. 89, Issue 3 https://doi.org/10.1007/s00253-010-2887-6	journal	October 2010
PduP is a coenzyme-a-acylating propionaldehyde dehydrogenase associated with the polyhedral bodies involved in B 12 -dependent 1,2-propanediol degradation by Salmonella enterica serovar Typhimurium LT2 Leal, Nicole A.; Havemann, Gregory D.; Bobik, Thomas A. Archives of Microbiology, Vol. 180, Issue 5 https://doi.org/10.1007/s00203-003-0601-0	journal	November 2003
Characterization and Detection of a Widely Distributed Gene Cluster That Predicts Anaerobic Choline Utilization by Human Gut Bacteria Martínez-del Campo, Ana; Bodea, Smaranda; Hamer, Hilary A. mBio, Vol. 6, Issue 2 https://doi.org/10.1128/mBio.00042-15	journal	April 2015
Steady-state and pre-steady-state kinetics of coenzyme A-linked aldehyde dehydrogenase from Escherichia coli Shone, Clifford C.; Fromm, Herbert J. Biochemistry, Vol. 20, Issue 26 https://doi.org/10.1021/bi00529a026	journal	December 1981
The Structural Basis of Coenzyme A Recycling in a Bacterial Organelle Erbilgin, Onur; Sutter, Markus; Kerfeld, Cheryl A. PLOS Biology, Vol. 14, Issue 3 https://doi.org/10.1371/journal.pbio.1002399	journal	March 2016
The Structural Basis of Coenzyme A Recycling in a Bacterial Organelle Erbilgin, Onur; Sutter, Markus; Kerfeld, Cheryl A. PLOS Biology, Vol. 14, Issue 3 https://doi.org/10.1371/journal.pbio.1002399	journal	March 2016
Investigation of the Active Site Cysteine Residue of Rat Liver Mitochondrial Aldehyde Dehydrogenase by Site-Directed Mutagenesis Farres, Jaume; Wang, Thomas T. Y.; Cunningham, Suzanne J. Biochemistry, Vol. 34, Issue 8 https://doi.org/10.1021/bi00008a025	journal	February 1995
Cutting Choline with Radical Scissors Broderick, Joan B.; Moody, James D. Cell Chemical Biology, Vol. 23, Issue 10 https://doi.org/10.1016/j.chembiol.2016.10.002	journal	October 2016
Employing bacterial microcompartment technology to engineer a shell-free enzyme-aggregate for enhanced 1,2-propanediol production in Escherichia coli Lee, Matthew J.; Brown, Ian R.; Juodeikis, Rokas Metabolic Engineering, Vol. 36 https://doi.org/10.1016/j.ymben.2016.02.007	journal	July 2016
Bacterial microcompartment assembly: The key role of encapsulation peptides Aussignargues, Clément; Paasch, Bradley C.; Gonzalez-Esquer, Raul Communicative & Integrative Biology, Vol. 8, Issue 3 https://doi.org/10.1080/19420889.2015.1039755	journal	May 2015
Involvement of a Bacterial Microcompartment in the Metabolism of Fucose and Rhamnose by Clostridium phytofermentans Petit, Elsa; LaTouf, W. Greg; Coppi, Maddalena V. PLoS ONE, Vol. 8, Issue 1 https://doi.org/10.1371/journal.pone.0054337	journal	January 2013
Insight into the Mechanism of the B ₁₂ -Independent Glycerol Dehydratase from Clostridium butyricum : Preliminary Biochemical and Structural Characterization ^‡ O'Brien, Jessica Rae; Raynaud, Celine; Croux, Christian Biochemistry, Vol. 43, Issue 16 https://doi.org/10.1021/bi035930k	journal	April 2004
Crystal Structures of Ethanolamine Ammonia-lyase Complexed with Coenzyme B ₁₂ Analogs and Substrates Shibata, Naoki; Tamagaki, Hiroko; Hieda, Naoki Journal of Biological Chemistry, Vol. 285, Issue 34 https://doi.org/10.1074/jbc.M110.125112	journal	June 2010
Bacterial microcompartments as metabolic modules for plant synthetic biology Gonzalez-Esquer, C. Raul; Newnham, Sarah E.; Kerfeld, Cheryl A. The Plant Journal, Vol. 87, Issue 1 https://doi.org/10.1111/tpj.13166	journal	June 2016
Characterization of a Planctomycetal Organelle: a Novel Bacterial Microcompartment for the Aerobic Degradation of Plant Saccharides Erbilgin, Onur; McDonald, Kent L.; Kerfeld, Cheryl A. Applied and Environmental Microbiology, Vol. 80, Issue 7 https://doi.org/10.1128/AEM.03887-13	journal	January 2014
Coenzyme A-acylating aldehyde dehydrogenase from Clostridium beijerinckii NRRL B592. Yan, R. T.; Chen, J. S. Applied and Environmental Microbiology, Vol. 56, Issue 9 https://doi.org/10.1128/aem.56.9.2591-2599.1990	journal	January 1990

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