skip to main content

DOE PAGESDOE PAGES

Title: Comparison of Thaumarchaeotal populations from four deep sea basins

The nitrogen cycle in the marine environment is strongly affected by ammonia-oxidizing Thaumarchaeota. In some marine settings, Thaumarchaeotes can comprise a large percentage of the prokaryotic population. To better understand the biogeographic patterns of Thaumarchaeotes, we sought to investigate differences in their abundance and phylogenetic diversity between geographically distinct basins. Samples were collected from four marine basins (The Caspian Sea, the Great Australian Bight, and the Central and Eastern Mediterranean). The concentration of bacterial and archaeal 16S rRNA genes and archaeal amoA genes were assessed using qPCR. Minimum entropy decomposition was used to elucidate the fine-scale diversity of Thaumarchaeotes. We demonstrated that there were significant differences in the abundance and diversity of Thaumarchaeotes between these four basins. The diversity of Thaumarchaeotal oligotypes differed between basins with many oligotypes only present in one of the four basins, which suggests that their distribution showed biogeographic patterning. There were also significant differences in Thaumarchaeotal community structure between these basins. This would suggest that geographically distant, yet geochemically similar basins may house distinct Thaumarchaeaotal populations. In conclusion, these findings suggest that Thaumarchaeota are very diverse and that biogeography in part contributes in determining the diversity and distribution of Thaumarchaeotes.
Authors:
 [1] ;  [2] ;  [2] ;  [3] ;  [4] ;  [4] ;  [5]
  1. Michigan Technological Univ., Houghton, MI (United States). Department of Biological Sciences
  2. Univ. of Tennessee, Knoxville, TN (United States). Department of Civil and Environmental Engineering
  3. Univ. of Tennessee, Knoxville, TN (United States). Center for Environmental Biotechnology and Bredesen Center
  4. Univ. of Tennessee, Knoxville, TN (United States). Department of Civil and Environmental Engineering and Center for Environmental Biotechnology
  5. Univ. of Tennessee, Knoxville, TN (United States). Department of Civil and Environmental Engineering, Center for Environmental Biotechnology, Bredesen Center, Department of Earth and Planetary Sciences, Department of Microbiology, and Institute for a Secure and Sustainable Environment; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Biosciences Division
Publication Date:
Grant/Contract Number:
AC05-00OR22725
Type:
Accepted Manuscript
Journal Name:
FEMS Microbiology Ecology (Online)
Additional Journal Information:
Journal Name: FEMS Microbiology Ecology (Online); Journal Volume: 93; Journal Issue: 11; Journal ID: ISSN 1574-6941
Publisher:
Federation of European Microbiological Societies
Research Org:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 54 ENVIRONMENTAL SCIENCES; Thaumarchaeota; qPCR; minimum entropy decomposition; biogeography
OSTI Identifier:
1415207

Techtman, Stephen M., Mahmoudi, Nagissa, Whitt, Kendall T., Campa, Maria Fernanda, Fortney, Julian L., Joyner, Dominique C., and Hazen, Terry C.. Comparison of Thaumarchaeotal populations from four deep sea basins. United States: N. p., Web. doi:10.1093/femsec/fix128.
Techtman, Stephen M., Mahmoudi, Nagissa, Whitt, Kendall T., Campa, Maria Fernanda, Fortney, Julian L., Joyner, Dominique C., & Hazen, Terry C.. Comparison of Thaumarchaeotal populations from four deep sea basins. United States. doi:10.1093/femsec/fix128.
Techtman, Stephen M., Mahmoudi, Nagissa, Whitt, Kendall T., Campa, Maria Fernanda, Fortney, Julian L., Joyner, Dominique C., and Hazen, Terry C.. 2017. "Comparison of Thaumarchaeotal populations from four deep sea basins". United States. doi:10.1093/femsec/fix128. https://www.osti.gov/servlets/purl/1415207.
@article{osti_1415207,
title = {Comparison of Thaumarchaeotal populations from four deep sea basins},
author = {Techtman, Stephen M. and Mahmoudi, Nagissa and Whitt, Kendall T. and Campa, Maria Fernanda and Fortney, Julian L. and Joyner, Dominique C. and Hazen, Terry C.},
abstractNote = {The nitrogen cycle in the marine environment is strongly affected by ammonia-oxidizing Thaumarchaeota. In some marine settings, Thaumarchaeotes can comprise a large percentage of the prokaryotic population. To better understand the biogeographic patterns of Thaumarchaeotes, we sought to investigate differences in their abundance and phylogenetic diversity between geographically distinct basins. Samples were collected from four marine basins (The Caspian Sea, the Great Australian Bight, and the Central and Eastern Mediterranean). The concentration of bacterial and archaeal 16S rRNA genes and archaeal amoA genes were assessed using qPCR. Minimum entropy decomposition was used to elucidate the fine-scale diversity of Thaumarchaeotes. We demonstrated that there were significant differences in the abundance and diversity of Thaumarchaeotes between these four basins. The diversity of Thaumarchaeotal oligotypes differed between basins with many oligotypes only present in one of the four basins, which suggests that their distribution showed biogeographic patterning. There were also significant differences in Thaumarchaeotal community structure between these basins. This would suggest that geographically distant, yet geochemically similar basins may house distinct Thaumarchaeaotal populations. In conclusion, these findings suggest that Thaumarchaeota are very diverse and that biogeography in part contributes in determining the diversity and distribution of Thaumarchaeotes.},
doi = {10.1093/femsec/fix128},
journal = {FEMS Microbiology Ecology (Online)},
number = 11,
volume = 93,
place = {United States},
year = {2017},
month = {10}
}