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Title: Undefined cellulase formulations hinder scientific reproducibility

In the shadow of a burgeoning biomass-to-fuels industry, biological conversion of lignocellulose to fermentable sugars in a cost-effective manner is key to the success of second-generation and advanced biofuel production. For the effective comparison of one cellulase preparation to another, cellulase assays are typically carried out with one or more engineered cellulase formulations or natural exoproteomes of known performance serving as positive controls. When these formulations have unknown composition, as is the case with several widely used commercial products, it becomes impossible to compare or reproduce work done today to work done in the future, where, for example, such preparations may not be available. Therefore, being a critical tenet of science publishing, experimental reproducibility is endangered by the continued use of these undisclosed products. We propose the introduction of standard procedures and materials to produce specific and reproducible cellulase formulations. These formulations are to serve as yardsticks to measure improvements and performance of new cellulase formulations.
 [1] ;  [2] ;  [1] ;  [3] ;  [1] ;  [1] ;  [1] ;  [4] ;  [5] ;  [6] ;  [7] ;  [8] ;  [2] ;  [6] ;  [1]
  1. National Renewable Energy Lab. (NREL), Golden, CO (United States). Biosciences Center
  2. Univ. of Illinois, Urbana, IL (United States). Department of Food Science and Human Nutrition
  3. The Weizmann Institute of Science, Rehovot (Israel). Department of Biomolecular Sciences
  4. University of Copenhagen, Frederiksberg C (Denmark). Department of Geosciences and Natural Resource Management
  5. Univ. of California, Davis, CA (United States). Department of Biological and Agricultural Engineering
  6. University of California, Riverside, CA (United States). Bourns College of Engineering Center for Environmental Research and Technology
  7. Megazyme, Co. Wicklow (Ireland)
  8. Rhodes University, Grahamstown (South Africa). Department of Biochemistry and Microbiology
Publication Date:
Report Number(s):
Journal ID: ISSN 1754-6834
Grant/Contract Number:
Accepted Manuscript
Journal Name:
Biotechnology for Biofuels
Additional Journal Information:
Journal Volume: 10; Journal Issue: 1; Journal ID: ISSN 1754-6834
BioMed Central
Research Org:
National Renewable Energy Lab. (NREL), Golden, CO (United States)
Sponsoring Org:
USDOE Office of Energy Efficiency and Renewable Energy (EERE), Bioenergy Technologies Office (EE-3B)
Country of Publication:
United States
09 BIOMASS FUELS; cellulase; cellulose; biofuels; assays; commercial cellulase formulations
OSTI Identifier: