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Title: Field-grown miR156 transgenic switchgrass reproduction, yield, global gene expression analysis, and bioconfinement

Genetic engineering has been effective in altering cell walls for biofuel production in the bioenergy crop, switchgrass (Panicum virgatum). However, regulatory issues arising from gene flow may prevent commercialization of engineered switchgrass in the eastern United States where the species is native. And, depending on its expression level, microRNA156 (miR156) can reduce, delay, or eliminate flowering, which may serve to decrease transgene flow. Here, in this unique field study of transgenic switchgrass that was permitted to flower, two low (T14 and T35) and two medium (T27 and T37) miR156-overexpressing 'Alamo' lines with the transgene under the control of the constitutive maize (Zea mays) ubiquitin 1 promoter, along with nontransgenic control plants, were grown in eastern Tennessee over two seasons.
Authors:
 [1] ;  [2] ;  [3] ;  [3] ;  [4] ;  [4] ; ORCiD logo [4] ;  [1] ;  [3] ; ORCiD logo [2]
  1. Univ. of Tennessee, Knoxville, TN (United States). Dept. of Plant Sciences
  2. Univ. of Tennessee, Knoxville, TN (United States). Dept. of Plant Sciences; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC). BioEnergy Science Center
  3. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC). BioEnergy Science Center; Noble Research Inst., Ardmore, OK (United States)
  4. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC). BioEnergy Science Center; National Renewable Energy Lab. (NREL), Golden, CO (United States)
Publication Date:
Report Number(s):
NREL/JA-2700-70667
Journal ID: ISSN 1754-6834
Grant/Contract Number:
AC36-08GO28308
Type:
Accepted Manuscript
Journal Name:
Biotechnology for Biofuels
Additional Journal Information:
Journal Volume: 10; Journal Issue: 1; Journal ID: ISSN 1754-6834
Publisher:
BioMed Central
Research Org:
National Renewable Energy Lab. (NREL), Golden, CO (United States)
Sponsoring Org:
USDOE Office of Science (SC), Office of Biological and Environmental Research; United States Department of Agriculture (USDA)
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; bioconfinement; floral transition; miR156; switchgrass; gene flow
OSTI Identifier:
1414068