The effect of switchgrass loadings on feedstock solubilization and biofuel production by Clostridium thermocellum
Abstract
High solids loading fermentations are necessary for the industrialization of lignocellulosic ethanol. To date, only a few studies have investigated the effect of solids loadings on microorganisms of interest for consolidated bioprocessing (CBP). Here, the effect that various switchgrass loadings have on Clostridium thermocellum solubilization and bioconversion are investigated. C. thermocellum was grown for ten days on 10, 25 or 50 g/L switchgrass or Avicel at equivalent glucan loadings. Avicel was completely consumed at all loadings, but total cellulose solubilization decreased from 63% to 37% as switchgrass loadings increased from 10 g/L to 50 g/L. Washed, spent switchgrass could be additionally hydrolyzed and fermented in second-round fermentations suggesting access to fermentable substrates was not the limiting factor at higher feedstock loadings. Fermentations of Avicel or cellobiose using culture medium supplemented with 50% spent fermentation broth identified that compounds present in the samples collected from the 25 or 50 g/L switchgrass loadings were the most inhibitory to continued fermentation. Finally, recalcitrance alone cannot fully account for differences in solubilization and end-production formation between switchgrass and Avicel at increased substrate loadings. Effort to decouple metabolic inhibition from inhibition of hydrolysis suggest that C. thermocellum’s hydrolytic machinery is more vulnerable to inhibition frommore »
- Authors:
- Publication Date:
- Research Org.:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- OSTI Identifier:
- 1618690
- Alternate Identifier(s):
- OSTI ID: 1413625
- Grant/Contract Number:
- PS02-06ER64304; AC05-00OR22725
- Resource Type:
- Published Article
- Journal Name:
- Biotechnology for Biofuels
- Additional Journal Information:
- Journal Name: Biotechnology for Biofuels Journal Volume: 10 Journal Issue: 1; Journal ID: ISSN 1754-6834
- Publisher:
- Springer Science + Business Media
- Country of Publication:
- Netherlands
- Language:
- English
- Subject:
- 09 BIOMASS FUELS; Clostridium thermocellum; Consolidated bioprocessing; Switchgrass; Recalcitrance; Inhibition; High-solid loading; Ethanol
Citation Formats
Verbeke, Tobin J., Garcia, Gabriela M., and Elkins, James G. The effect of switchgrass loadings on feedstock solubilization and biofuel production by Clostridium thermocellum. Netherlands: N. p., 2017.
Web. doi:10.1186/s13068-017-0917-7.
Verbeke, Tobin J., Garcia, Gabriela M., & Elkins, James G. The effect of switchgrass loadings on feedstock solubilization and biofuel production by Clostridium thermocellum. Netherlands. https://doi.org/10.1186/s13068-017-0917-7
Verbeke, Tobin J., Garcia, Gabriela M., and Elkins, James G. Thu .
"The effect of switchgrass loadings on feedstock solubilization and biofuel production by Clostridium thermocellum". Netherlands. https://doi.org/10.1186/s13068-017-0917-7.
@article{osti_1618690,
title = {The effect of switchgrass loadings on feedstock solubilization and biofuel production by Clostridium thermocellum},
author = {Verbeke, Tobin J. and Garcia, Gabriela M. and Elkins, James G.},
abstractNote = {High solids loading fermentations are necessary for the industrialization of lignocellulosic ethanol. To date, only a few studies have investigated the effect of solids loadings on microorganisms of interest for consolidated bioprocessing (CBP). Here, the effect that various switchgrass loadings have on Clostridium thermocellum solubilization and bioconversion are investigated. C. thermocellum was grown for ten days on 10, 25 or 50 g/L switchgrass or Avicel at equivalent glucan loadings. Avicel was completely consumed at all loadings, but total cellulose solubilization decreased from 63% to 37% as switchgrass loadings increased from 10 g/L to 50 g/L. Washed, spent switchgrass could be additionally hydrolyzed and fermented in second-round fermentations suggesting access to fermentable substrates was not the limiting factor at higher feedstock loadings. Fermentations of Avicel or cellobiose using culture medium supplemented with 50% spent fermentation broth identified that compounds present in the samples collected from the 25 or 50 g/L switchgrass loadings were the most inhibitory to continued fermentation. Finally, recalcitrance alone cannot fully account for differences in solubilization and end-production formation between switchgrass and Avicel at increased substrate loadings. Effort to decouple metabolic inhibition from inhibition of hydrolysis suggest that C. thermocellum’s hydrolytic machinery is more vulnerable to inhibition from switchgrass-derived inhibitors than is the bacterium’s metabolism.},
doi = {10.1186/s13068-017-0917-7},
journal = {Biotechnology for Biofuels},
number = 1,
volume = 10,
place = {Netherlands},
year = {Thu Nov 30 00:00:00 EST 2017},
month = {Thu Nov 30 00:00:00 EST 2017}
}
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https://doi.org/10.1186/s13068-017-0917-7
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