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Title: Primary and heterotrophic productivity relate to multikingdom diversity in a hypersaline mat

Abstract

Benthic microbial ecosystems are widespread yet knowledge gaps still remain on the relationships between the diversity of species across kingdoms and productivity. Here, we ask two fundamental questions: (i) How does species diversity relate to the rates of primary and heterotrophic productivity? (ii) How do diel variations in light-energy inputs influence productivity and microbiome diversity? To answer these questions, microbial mats from a magnesium sulfate hypersaline lake were used to establish microcosms. Both the number and relatedness between bacterial and eukaryotic taxa in the microbiome were assayed via amplicon-based sequencing of 16S and 18S rRNA genes over two diel cycles. These results correlated with biomass productivity obtained from substrate-specific 13C stable isotope tracers that enabled comparisons between primary and heterotrophic productivity. Both bacterial and eukaryotic species richness and evenness were related only to the rates of 13C-labeled glucose and acetate biomass incorporation. Interestingly, measures of these heterotrophic relationships changed from positive and negative correlations depending on carbon derived from glucose or acetate, respectively. The bacterial and eukaryotic diversity of this ecosystem is also controlled, in part, from energy constraints imposed by changing irradiance over a diel cycle.

Authors:
ORCiD logo [1];  [2];  [3];  [2];  [3];  [2];  [2];  [3]
  1. Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Biological Sciences Division; Washington State Univ., Pullman, WA (United States). The Gene and Linda Voiland School of Chemical Engineering and Bioengineering
  2. Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Biological Sciences Division
  3. Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Signature Science and Technology Division
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER)
OSTI Identifier:
1413512
Report Number(s):
PNNL-SA-127139
Journal ID: ISSN 1574-6941
Grant/Contract Number:  
AC05-76RL01830
Resource Type:
Accepted Manuscript
Journal Name:
FEMS Microbiology Ecology (Online)
Additional Journal Information:
Journal Name: FEMS Microbiology Ecology (Online); Journal Volume: 93; Journal Issue: 10; Journal ID: ISSN 1574-6941
Publisher:
Federation of European Microbiological Societies
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 54 ENVIRONMENTAL SCIENCES; diversity; productivity; microbial mat; stable isotope; hypersaline

Citation Formats

Bernstein, Hans C., Brislawn, Colin J., Dana, Karl, Flores-Wentz, Tobias, Cory, Alexandra B., Fansler, Sarah J., Fredrickson, James K., and Moran, James J.. Primary and heterotrophic productivity relate to multikingdom diversity in a hypersaline mat. United States: N. p., 2017. Web. https://doi.org/10.1093/FEMSEC/FIX121.
Bernstein, Hans C., Brislawn, Colin J., Dana, Karl, Flores-Wentz, Tobias, Cory, Alexandra B., Fansler, Sarah J., Fredrickson, James K., & Moran, James J.. Primary and heterotrophic productivity relate to multikingdom diversity in a hypersaline mat. United States. https://doi.org/10.1093/FEMSEC/FIX121
Bernstein, Hans C., Brislawn, Colin J., Dana, Karl, Flores-Wentz, Tobias, Cory, Alexandra B., Fansler, Sarah J., Fredrickson, James K., and Moran, James J.. Tue . "Primary and heterotrophic productivity relate to multikingdom diversity in a hypersaline mat". United States. https://doi.org/10.1093/FEMSEC/FIX121. https://www.osti.gov/servlets/purl/1413512.
@article{osti_1413512,
title = {Primary and heterotrophic productivity relate to multikingdom diversity in a hypersaline mat},
author = {Bernstein, Hans C. and Brislawn, Colin J. and Dana, Karl and Flores-Wentz, Tobias and Cory, Alexandra B. and Fansler, Sarah J. and Fredrickson, James K. and Moran, James J.},
abstractNote = {Benthic microbial ecosystems are widespread yet knowledge gaps still remain on the relationships between the diversity of species across kingdoms and productivity. Here, we ask two fundamental questions: (i) How does species diversity relate to the rates of primary and heterotrophic productivity? (ii) How do diel variations in light-energy inputs influence productivity and microbiome diversity? To answer these questions, microbial mats from a magnesium sulfate hypersaline lake were used to establish microcosms. Both the number and relatedness between bacterial and eukaryotic taxa in the microbiome were assayed via amplicon-based sequencing of 16S and 18S rRNA genes over two diel cycles. These results correlated with biomass productivity obtained from substrate-specific 13C stable isotope tracers that enabled comparisons between primary and heterotrophic productivity. Both bacterial and eukaryotic species richness and evenness were related only to the rates of 13C-labeled glucose and acetate biomass incorporation. Interestingly, measures of these heterotrophic relationships changed from positive and negative correlations depending on carbon derived from glucose or acetate, respectively. The bacterial and eukaryotic diversity of this ecosystem is also controlled, in part, from energy constraints imposed by changing irradiance over a diel cycle.},
doi = {10.1093/FEMSEC/FIX121},
journal = {FEMS Microbiology Ecology (Online)},
number = 10,
volume = 93,
place = {United States},
year = {2017},
month = {10}
}

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    Works referencing / citing this record:

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