Quantification of triglyceride content in oleaginous materials using thermo-gravimetry

Maddi, Balakrishna; Vadlamani, Agasteswar; Viamajala, Sridhar; Varanasi, Sasidhar

doi:10.1016/J.JAAP.2017.10.006

Title: Quantification of triglyceride content in oleaginous materials using thermo-gravimetry

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Abstract

Laboratory analytical methods for quantification of triglyceride content in oleaginous biomass samples, especially microalgae, require toxic chemicals and/or organic solvents and involve multiple steps. We describe a simple triglyceride quantification method that uses thermo-gravimetry. This method is based on the observation that triglycerides undergo near-complete volatilization/degradation over a narrow temperature interval with a derivative weight loss peak at 420 °C when heated in an inert atmosphere. Degradation of the other constituents of oleaginous biomass (protein and carbohydrates) is largely complete after prolonged exposure of samples at 320 °C. Based on these observations, the triglyceride content of oleaginous biomass was estimated by using the following two-step process. In Step 1, samples were heated to 320 °C and kept isothermal at this temperature for 15 min. In Step 2, samples were heated from 320 °C to 420 °C and then kept isothermal at 420 °C for 15 min. The results show that mass loss in step 2 correlated well with triglyceride content estimates obtained from conventional techniques for diverse microalgae and oilseed samples.

Authors:

Maddi, Balakrishna ^[1]; Vadlamani, Agasteswar ^[2]; Viamajala, Sridhar ^[2]; Varanasi, Sasidhar ^[3]

Univ. of Toledo, OH (United States). Dept. of Chemical and Environmental Engineering; Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Univ. of Toledo, OH (United States). Dept. of Chemical and Environmental Engineering
Univ. of Toledo, OH (United States). Dept. of Chemical and Environmental Engineering; Manhanttan College, Riverdale, NY (United States). Dept. of Chemical Engineering

Publication Date:: 2017-10-16

Research Org.:: Pacific Northwest National Lab. (PNNL), Richland, WA (United States)

Sponsoring Org.:: USDOE Office of Energy Efficiency and Renewable Energy (EERE), Sustainable Transportation Office. Bioenergy Technologies Office

OSTI Identifier:: 1413483

Grant/Contract Number:: AC0576RL01830; CHE-1230609; EE0005993

Resource Type:: Accepted Manuscript

Journal Name:: Journal of Analytical and Applied Pyrolysis

Additional Journal Information:: Journal Volume: 128; Journal Issue: C; Journal ID: ISSN 0165-2370

Publisher:: Elsevier

Country of Publication:: United States

Language:: English

Subject:: 60 APPLIED LIFE SCIENCES; 09 BIOMASS FUELS; triglyceride quantification; lipid analysis; thermo-gravimetry; microalgae; biomass; oilseeds

Citation Formats


                    Maddi, Balakrishna, Vadlamani, Agasteswar, Viamajala, Sridhar, and Varanasi, Sasidhar. Quantification of triglyceride content in oleaginous materials using thermo-gravimetry.  United States: N. p., 2017. 
Web.  doi:10.1016/J.JAAP.2017.10.006.

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                    Maddi, Balakrishna, Vadlamani, Agasteswar, Viamajala, Sridhar, & Varanasi, Sasidhar. Quantification of triglyceride content in oleaginous materials using thermo-gravimetry.  United States.  https://doi.org/10.1016/J.JAAP.2017.10.006

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                    Maddi, Balakrishna, Vadlamani, Agasteswar, Viamajala, Sridhar, and Varanasi, Sasidhar. Mon .  
"Quantification of triglyceride content in oleaginous materials using thermo-gravimetry".  United States.  https://doi.org/10.1016/J.JAAP.2017.10.006.  https://www.osti.gov/servlets/purl/1413483.

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@article{osti_1413483,

  title        = {Quantification of triglyceride content in oleaginous materials using thermo-gravimetry},

  author       = {Maddi, Balakrishna and Vadlamani, Agasteswar and Viamajala, Sridhar and Varanasi, Sasidhar},

  abstractNote = {Laboratory analytical methods for quantification of triglyceride content in oleaginous biomass samples, especially microalgae, require toxic chemicals and/or organic solvents and involve multiple steps. We describe a simple triglyceride quantification method that uses thermo-gravimetry. This method is based on the observation that triglycerides undergo near-complete volatilization/degradation over a narrow temperature interval with a derivative weight loss peak at 420 °C when heated in an inert atmosphere. Degradation of the other constituents of oleaginous biomass (protein and carbohydrates) is largely complete after prolonged exposure of samples at 320 °C. Based on these observations, the triglyceride content of oleaginous biomass was estimated by using the following two-step process. In Step 1, samples were heated to 320 °C and kept isothermal at this temperature for 15 min. In Step 2, samples were heated from 320 °C to 420 °C and then kept isothermal at 420 °C for 15 min. The results show that mass loss in step 2 correlated well with triglyceride content estimates obtained from conventional techniques for diverse microalgae and oilseed samples.},

  doi          = {10.1016/J.JAAP.2017.10.006},

  journal      = {Journal of Analytical and Applied Pyrolysis},

  number       = C,

  volume       = 128,

  place        = {United States},

  year         = {2017},

  month        = {10}

}

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