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Title: Mesophyll conductance in Zea mays responds transiently to CO 2 availability: implications for transpiration efficiency in C 4 crops

Abstract

Summary Mesophyll conductance ( g m ) describes the movement of CO 2 from the intercellular air spaces below the stomata to the site of initial carboxylation in the mesophyll. In contrast with C 3 ‐ g m , little is currently known about the intraspecific variation in C 4 ‐ g m or its responsiveness to environmental stimuli. To address these questions, g m was measured on five maize ( Zea mays ) lines in response to CO 2 , employing three different estimates of g m . Each of the methods indicated a significant response of g m to CO 2 . Estimates of g m were similar between methods at ambient and higher CO 2 , but diverged significantly at low partial pressures of CO 2 . These differences are probably driven by incomplete chemical and isotopic equilibrium between CO 2 and bicarbonate under these conditions. Carbonic anhydrase and phosphoenolpyruvate carboxylase in vitro activity varied significantly despite similar values of g m and leaf anatomical traits. These results provide strong support for a CO 2 response of g m in Z. mays , and indicate that g m in maize is probably driven by anatomical constraints rather than bymore » biochemical limitations. The CO 2 response of g m indicates a potential role for facilitated diffusion in C 4 ‐ g m . These results also suggest that water‐use efficiency could be enhanced in C 4 species by targeting g m .« less

Authors:
 [1];  [1]
  1. School of Biological Sciences Washington State University Pullman WA 99164 USA
Publication Date:
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER)
OSTI Identifier:
1412583
Grant/Contract Number:  
SC0008769
Resource Type:
Publisher's Accepted Manuscript
Journal Name:
New Phytologist
Additional Journal Information:
Journal Name: New Phytologist Journal Volume: 217 Journal Issue: 4; Journal ID: ISSN 0028-646X
Publisher:
Wiley-Blackwell
Country of Publication:
United Kingdom
Language:
English

Citation Formats

Kolbe, Allison R., and Cousins, Asaph B. Mesophyll conductance in Zea mays responds transiently to CO 2 availability: implications for transpiration efficiency in C 4 crops. United Kingdom: N. p., 2017. Web. doi:10.1111/nph.14942.
Kolbe, Allison R., & Cousins, Asaph B. Mesophyll conductance in Zea mays responds transiently to CO 2 availability: implications for transpiration efficiency in C 4 crops. United Kingdom. https://doi.org/10.1111/nph.14942
Kolbe, Allison R., and Cousins, Asaph B. Fri . "Mesophyll conductance in Zea mays responds transiently to CO 2 availability: implications for transpiration efficiency in C 4 crops". United Kingdom. https://doi.org/10.1111/nph.14942.
@article{osti_1412583,
title = {Mesophyll conductance in Zea mays responds transiently to CO 2 availability: implications for transpiration efficiency in C 4 crops},
author = {Kolbe, Allison R. and Cousins, Asaph B.},
abstractNote = {Summary Mesophyll conductance ( g m ) describes the movement of CO 2 from the intercellular air spaces below the stomata to the site of initial carboxylation in the mesophyll. In contrast with C 3 ‐ g m , little is currently known about the intraspecific variation in C 4 ‐ g m or its responsiveness to environmental stimuli. To address these questions, g m was measured on five maize ( Zea mays ) lines in response to CO 2 , employing three different estimates of g m . Each of the methods indicated a significant response of g m to CO 2 . Estimates of g m were similar between methods at ambient and higher CO 2 , but diverged significantly at low partial pressures of CO 2 . These differences are probably driven by incomplete chemical and isotopic equilibrium between CO 2 and bicarbonate under these conditions. Carbonic anhydrase and phosphoenolpyruvate carboxylase in vitro activity varied significantly despite similar values of g m and leaf anatomical traits. These results provide strong support for a CO 2 response of g m in Z. mays , and indicate that g m in maize is probably driven by anatomical constraints rather than by biochemical limitations. The CO 2 response of g m indicates a potential role for facilitated diffusion in C 4 ‐ g m . These results also suggest that water‐use efficiency could be enhanced in C 4 species by targeting g m .},
doi = {10.1111/nph.14942},
journal = {New Phytologist},
number = 4,
volume = 217,
place = {United Kingdom},
year = {Fri Dec 08 00:00:00 EST 2017},
month = {Fri Dec 08 00:00:00 EST 2017}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
https://doi.org/10.1111/nph.14942

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Cited by: 25 works
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