Adding tetrahydrofuran to dilute acid pretreatment provides new insights into substrate changes that greatly enhance biomass deconstruction by Clostridium thermocellum and fungal enzymes

Thomas, Vanessa A.; Donohoe, Bryon S.; Li, Mi; Pu, Yunqiao; Ragauskas, Arthur J.; Kumar, Rajeev; Nguyen, Thanh Yen; Cai, Charles M.; Wyman, Charles E.

doi:10.1186/s13068-017-0937-3

Title: Adding tetrahydrofuran to dilute acid pretreatment provides new insights into substrate changes that greatly enhance biomass deconstruction by Clostridium thermocellum and fungal enzymes

Journal Article · 30 November 2017 · Biotechnology for Biofuels

DOI: https://doi.org/10.1186/s13068-017-0937-3 · OSTI ID:1412036

Thomas, Vanessa A. ^[1]; Donohoe, Bryon S. ^[2]; Li, Mi ^[3]; Pu, Yunqiao ^[3]; Ragauskas, Arthur J. ^[4]; Kumar, Rajeev ^[1]; Nguyen, Thanh Yen ^[5]; Cai, Charles M. ^[1];

^[1]

Univ. of California, Riverside, CA (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
National Renewable Energy Lab. (NREL), Golden, CO (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Univ. of Tennessee, Knoxville, TN (United States)
Univ. of California, Riverside, CA (United States)

Consolidated bioprocessing (CBP) by anaerobes, such as Clostridium thermocellum, which combine enzyme production, hydrolysis, and fermentation are promising alternatives to historical economic challenges of using fungal enzymes for biological conversion of lignocellulosic biomass. However, limited research has integrated CBP with real pretreated biomass, and understanding how pretreatment impacts subsequent deconstruction by CBP vs. fungal enzymes can provide valuable insights into CBP and suggest other novel biomass deconstruction strategies. This study focused on determining the effect of pretreatment by dilute sulfuric acid alone (DA) and with tetrahydrofuran (THF) addition via co-solvent-enhanced lignocellulosic fractionation (CELF) on deconstruction of corn stover and Populus with much different recalcitrance by C. thermocellum vs. fungal enzymes and changes in pretreated biomass related to these differences.

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Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States); National Renewable Energy Laboratory (NREL), Golden, CO (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC05-00OR22725; AC36-08GO28308

OSTI ID:: 1412036

Alternate ID(s):: OSTI ID: 1414067

Report Number(s):: NREL/JA-2700-70675; 106233

Journal Information:: Biotechnology for Biofuels, Vol. 10, Issue 1; ISSN 1754-6834

Publisher:: BioMed CentralCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 35 works

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Elucidating Structural Characteristics of Biomass using Solution-State 2 D NMR with a Mixture of Deuterated Dimethylsulfoxide and Hexamethylphosphoramide Yoo, Chang Geun; Pu, Yunqiao; Li, Mi ChemSusChem, Vol. 9, Issue 10 https://doi.org/10.1002/cssc.201600135	journal	April 2016
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Conversion for Avicel and AFEX pretreated corn stover by Clostridium thermocellum and simultaneous saccharification and fermentation: Insights into microbial conversion of pretreated cellulosic biomass Shao, Xiongjun; Jin, Mingjie; Guseva, Anna Bioresource Technology, Vol. 102, Issue 17 https://doi.org/10.1016/j.biortech.2011.05.021	journal	September 2011
Comparative data on effects of leading pretreatments and enzyme loadings and formulations on sugar yields from different switchgrass sources Wyman, Charles E.; Balan, Venkatesh; Dale, Bruce E. Bioresource Technology, Vol. 102, Issue 24 https://doi.org/10.1016/j.biortech.2011.06.069	journal	December 2011
Formation of degradation compounds from lignocellulosic biomass in the biorefinery: sugar reaction mechanisms Rasmussen, Helena; Sørensen, Hanne R.; Meyer, Anne S. Carbohydrate Research, Vol. 385 https://doi.org/10.1016/j.carres.2013.08.029	journal	February 2014
The potential of cellulases and cellulosomes for cellulosic waste management Bayer, Edward A.; Lamed, Raphael; Himmel, Michael E. Current Opinion in Biotechnology, Vol. 18, Issue 3 https://doi.org/10.1016/j.copbio.2007.04.004	journal	June 2007
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Characterization and biotechnological application of recombinant xylanases from Aspergillus nidulans Maitan-Alfenas, Gabriela P.; Oliveira, Mariana B.; Nagem, Ronaldo A. P. International Journal of Biological Macromolecules, Vol. 91 https://doi.org/10.1016/j.ijbiomac.2016.05.065	journal	October 2016
What is vital (and not vital) to advance economically-competitive biofuels production Zhang, Y. -H. Percival Process Biochemistry, Vol. 46, Issue 11 https://doi.org/10.1016/j.procbio.2011.08.005	journal	November 2011
What is (and is not) vital to advancing cellulosic ethanol Wyman, Charles E. Trends in Biotechnology, Vol. 25, Issue 4 https://doi.org/10.1016/j.tibtech.2007.02.009	journal	April 2007
Wood-Derived Materials for Green Electronics, Biological Devices, and Energy Applications Zhu, Hongli; Luo, Wei; Ciesielski, Peter N. Chemical Reviews, Vol. 116, Issue 16 https://doi.org/10.1021/acs.chemrev.6b00225	journal	July 2016
Biocommodity Engineering Lynd, L. R.; Wyman, C. E.; Gerngross, T. U. Biotechnology Progress, Vol. 15, Issue 5 https://doi.org/10.1021/bp990109e	journal	October 1999
Production of Ethanol: By Thermophilic Fermentation of Cellulose Veldhuis, M. K.; Christensen, L. M.; Fulmer, Ellis I. Industrial & Engineering Chemistry, Vol. 28, Issue 4 https://doi.org/10.1021/ie50316a015	journal	April 1936
Local Phase Separation of Co-solvents Enhances Pretreatment of Biomass for Bioenergy Applications Mostofian, Barmak; Cai, Charles M.; Smith, Micholas Dean Journal of the American Chemical Society, Vol. 138, Issue 34 https://doi.org/10.1021/jacs.6b03285	journal	August 2016
Distinct Roles of Residual Xylan and Lignin in Limiting Enzymatic Hydrolysis of Organosolv Pretreated Loblolly Pine and Sweetgum Li, Mi; Tu, Maobing; Cao, Dongxu Journal of Agricultural and Food Chemistry, Vol. 61, Issue 3 https://doi.org/10.1021/jf304517w	journal	January 2013
Carbohydrate and lignin are simultaneously solubilized from unpretreated switchgrass by microbial action at high temperature Kataeva, Irina; Foston, Marcus B.; Yang, Sung-Jae Energy & Environmental Science, Vol. 6, Issue 7 https://doi.org/10.1039/c3ee40932e	journal	January 2013
Climate change and health costs of air emissions from biofuels and gasoline Hill, Jason; Polasky, Stephen; Nelson, Erik Proceedings of the National Academy of Sciences, Vol. 106, Issue 6 https://doi.org/10.1073/pnas.0812835106	journal	February 2009
Lignin content in natural Populus variants affects sugar release Studer, M. H.; DeMartini, J. D.; Davis, M. F. Proceedings of the National Academy of Sciences, Vol. 108, Issue 15, p. 6300-6305 https://doi.org/10.1073/pnas.1009252108	journal	March 2011
Carbon sequestration Lal, Rattan Philosophical Transactions of the Royal Society B: Biological Sciences, Vol. 363, Issue 1492 https://doi.org/10.1098/rstb.2007.2185	journal	August 2007
Dramatic performance of Clostridium thermocellum explained by its wide range of cellulase modalities Xu, Qi; Resch, Michael G.; Podkaminer, Kara Science Advances, Vol. 2, Issue 2 https://doi.org/10.1126/sciadv.1501254	journal	February 2016
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Construction of consolidated bio-saccharification biocatalyst and process optimization for highly efficient lignocellulose solubilization Liu, Shiyue; Liu, Ya-Jun; Feng, Yingang Biotechnology for Biofuels, Vol. 12, Issue 1 https://doi.org/10.1186/s13068-019-1374-2	journal	February 2019
Promoting enzymatic hydrolysis of lignocellulosic biomass by inexpensive soy protein Luo, Xiaolin; Liu, Jing; Zheng, Peitao Biotechnology for Biofuels, Vol. 12, Issue 1 https://doi.org/10.1186/s13068-019-1387-x	journal	March 2019
Construction of consolidated bio-saccharification biocatalyst and process optimization for highly efficient lignocellulose solubilization Liu, Shiyue; Liu, Ya-Jun; Feng, Yingang Biotechnology for Biofuels, Vol. 12, Issue 1 https://doi.org/10.1186/s13068-019-1374-2	journal	February 2019
Promoting enzymatic hydrolysis of lignocellulosic biomass by inexpensive soy protein Luo, Xiaolin; Liu, Jing; Zheng, Peitao Biotechnology for Biofuels, Vol. 12, Issue 1 https://doi.org/10.1186/s13068-019-1387-x	journal	March 2019

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Related Subjects

09 BIOMASS FUELS
consolidated bioprocessing
recalcitrance
yield
sugar
fractionation
tetrahydrofuran

MOESM1 of Adding tetrahydrofuran to dilute acid pretreatment provides new insights into substrate changes that greatly enhance biomass deconstruction by Clostridium thermocellum and fungal enzymes Thomas, Vanessa; Donohoe, Bryon; Li, Mi https://doi.org/10.6084/m9.figshare.5648746.v1 The current record is supplemented by this dataset	dataset	November 2017
Adding tetrahydrofuran to dilute acid pretreatment provides new insights into substrate changes that greatly enhance biomass deconstruction by Clostridium thermocellum and fungal enzymes [Supplemental Data] Thomas, Vanessa; Donohoe, Bryon; Li, Mi https://doi.org/10.6084/m9.figshare.c.3942298 The current record is supplemented by this dataset	dataset	November 2017
Adding tetrahydrofuran to dilute acid pretreatment provides new insights into substrate changes that greatly enhance biomass deconstruction by Clostridium thermocellum and fungal enzymes Thomas, Vanessa; Donohoe, Bryon; Li, Mi https://doi.org/10.6084/m9.figshare.c.3942298.v1 The current record is supplemented by this dataset	dataset	November 2017
MOESM1 of Adding tetrahydrofuran to dilute acid pretreatment provides new insights into substrate changes that greatly enhance biomass deconstruction by Clostridium thermocellum and fungal enzymes Thomas, Vanessa; Donohoe, Bryon; Li, Mi https://doi.org/10.6084/m9.figshare.5648746 The current record is supplemented by this text	text	January 2017