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Title: Adding tetrahydrofuran to dilute acid pretreatment provides new insights into substrate changes that greatly enhance biomass deconstruction by Clostridium thermocellum and fungal enzymes

Abstract

Consolidated bioprocessing (CBP) by anaerobes, such as Clostridium thermocellum, which combine enzyme production, hydrolysis, and fermentation are promising alternatives to historical economic challenges of using fungal enzymes for biological conversion of lignocellulosic biomass. However, limited research has integrated CBP with real pretreated biomass, and understanding how pretreatment impacts subsequent deconstruction by CBP vs. fungal enzymes can provide valuable insights into CBP and suggest other novel biomass deconstruction strategies. This study focused on determining the effect of pretreatment by dilute sulfuric acid alone (DA) and with tetrahydrofuran (THF) addition via co-solvent-enhanced lignocellulosic fractionation (CELF) on deconstruction of corn stover and Populus with much different recalcitrance by C. thermocellum vs. fungal enzymes and changes in pretreated biomass related to these differences.

Authors:
 [1];  [2];  [3];  [3];  [4];  [1];  [5];  [1]; ORCiD logo [1]
  1. Univ. of California, Riverside, CA (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
  2. National Renewable Energy Lab. (NREL), Golden, CO (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
  3. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
  4. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Univ. of Tennessee, Knoxville, TN (United States)
  5. Univ. of California, Riverside, CA (United States)
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); National Renewable Energy Lab. (NREL), Golden, CO (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1412036
Alternate Identifier(s):
OSTI ID: 1414067
Report Number(s):
NREL/JA-2700-70675
Journal ID: ISSN 1754-6834; 106233
Grant/Contract Number:  
AC05-00OR22725; AC36-08GO28308
Resource Type:
Accepted Manuscript
Journal Name:
Biotechnology for Biofuels
Additional Journal Information:
Journal Volume: 10; Journal Issue: 1; Journal ID: ISSN 1754-6834
Publisher:
BioMed Central
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; consolidated bioprocessing; recalcitrance; yield; sugar; fractionation; tetrahydrofuran

Citation Formats

Thomas, Vanessa A., Donohoe, Bryon S., Li, Mi, Pu, Yunqiao, Ragauskas, Arthur J., Kumar, Rajeev, Nguyen, Thanh Yen, Cai, Charles M., and Wyman, Charles E. Adding tetrahydrofuran to dilute acid pretreatment provides new insights into substrate changes that greatly enhance biomass deconstruction by Clostridium thermocellum and fungal enzymes. United States: N. p., 2017. Web. doi:10.1186/s13068-017-0937-3.
Thomas, Vanessa A., Donohoe, Bryon S., Li, Mi, Pu, Yunqiao, Ragauskas, Arthur J., Kumar, Rajeev, Nguyen, Thanh Yen, Cai, Charles M., & Wyman, Charles E. Adding tetrahydrofuran to dilute acid pretreatment provides new insights into substrate changes that greatly enhance biomass deconstruction by Clostridium thermocellum and fungal enzymes. United States. doi:10.1186/s13068-017-0937-3.
Thomas, Vanessa A., Donohoe, Bryon S., Li, Mi, Pu, Yunqiao, Ragauskas, Arthur J., Kumar, Rajeev, Nguyen, Thanh Yen, Cai, Charles M., and Wyman, Charles E. Thu . "Adding tetrahydrofuran to dilute acid pretreatment provides new insights into substrate changes that greatly enhance biomass deconstruction by Clostridium thermocellum and fungal enzymes". United States. doi:10.1186/s13068-017-0937-3. https://www.osti.gov/servlets/purl/1412036.
@article{osti_1412036,
title = {Adding tetrahydrofuran to dilute acid pretreatment provides new insights into substrate changes that greatly enhance biomass deconstruction by Clostridium thermocellum and fungal enzymes},
author = {Thomas, Vanessa A. and Donohoe, Bryon S. and Li, Mi and Pu, Yunqiao and Ragauskas, Arthur J. and Kumar, Rajeev and Nguyen, Thanh Yen and Cai, Charles M. and Wyman, Charles E.},
abstractNote = {Consolidated bioprocessing (CBP) by anaerobes, such as Clostridium thermocellum, which combine enzyme production, hydrolysis, and fermentation are promising alternatives to historical economic challenges of using fungal enzymes for biological conversion of lignocellulosic biomass. However, limited research has integrated CBP with real pretreated biomass, and understanding how pretreatment impacts subsequent deconstruction by CBP vs. fungal enzymes can provide valuable insights into CBP and suggest other novel biomass deconstruction strategies. This study focused on determining the effect of pretreatment by dilute sulfuric acid alone (DA) and with tetrahydrofuran (THF) addition via co-solvent-enhanced lignocellulosic fractionation (CELF) on deconstruction of corn stover and Populus with much different recalcitrance by C. thermocellum vs. fungal enzymes and changes in pretreated biomass related to these differences.},
doi = {10.1186/s13068-017-0937-3},
journal = {Biotechnology for Biofuels},
number = 1,
volume = 10,
place = {United States},
year = {2017},
month = {11}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record

Citation Metrics:
Cited by: 7 works
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Figures / Tables:

Fig. 1 Fig. 1: Material flow for dilute acid and CELF pretreatments of corn stover and poplar wood followed by biological deconstruction mediated of the pretreated and washed solids by Clostridium thermocellum CBP at 60°C or fungal enzymes at 50°C

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    Works referencing / citing this record:

    Adding tetrahydrofuran to dilute acid pretreatment provides new insights into substrate changes that greatly enhance biomass deconstruction by Clostridium thermocellum and fungal enzymes [Supplemental Data]
    dataset, November 2017

    • Thomas, Vanessa; Donohoe, Bryon; Li, Mi
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      Figures/Tables have been extracted from DOE-funded journal article accepted manuscripts.