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Title: Mix-and-diffuse serial synchrotron crystallography

Abstract

Unravelling the interaction of biological macromolecules with ligands and substrates at high spatial and temporal resolution remains a major challenge in structural biology. The development of serial crystallography methods at X-ray free-electron lasers and subsequently at synchrotron light sources allows new approaches to tackle this challenge. Here, a new polyimide tape drive designed for mix-and-diffuse serial crystallography experiments is reported. The structure of lysozyme bound by the competitive inhibitor chitotriose was determined using this device in combination with microfluidic mixers. The electron densities obtained from mixing times of 2 and 50 s show clear binding of chitotriose to the enzyme at a high level of detail. Here, the success of this approach shows the potential for high-throughput drug screening and even structural enzymology on short timescales at bright synchrotron light sources.

Authors:
ORCiD logo [1];  [1]; ORCiD logo [1];  [1];  [1]; ORCiD logo [2];  [3]; ORCiD logo [3]; ORCiD logo [4]; ORCiD logo [5];  [6]; ORCiD logo [3]; ORCiD logo [1];  [1]; ORCiD logo [1];  [1];  [1]; ORCiD logo [1]; ORCiD logo [3]; ORCiD logo [7] more »; ORCiD logo [8];  [1]; ORCiD logo [1];  [1]; ORCiD logo [1]; ORCiD logo [1]; ORCiD logo [1]; ORCiD logo [3];  [1]; ORCiD logo [1];  [1];  [9];  [1]; ORCiD logo [3]; ORCiD logo [1]; ORCiD logo [1] « less
+ Show Author Affiliations
  1. Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany)
  2. California State Univ., Northridge, CA (United States)
  3. Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany); Univ. of Hamburg, Hamburg (Germany)
  4. Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany); Norwegian Univ. of Science and Technology, Trondheim (Norway)
  5. Uppsala Univ., Uppsala (Sweden)
  6. Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany); European X-ray Free-Electron Laser Facility GmbH (XFEL), Schenefeld (Germany)
  7. Univ. of Oxford, Oxford (England)
  8. SLAC National Accelerator Lab., Menlo Park, CA (United States)
  9. Fachhochschule Lubeck, Lubeck (Germany)
Publication Date:
Research Org.:
SLAC National Accelerator Lab., Menlo Park, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1410554
Grant/Contract Number:  
AC02-76SF00515; FP7/2007-2013; ERC-614507-Küpper; DFG-EXC1074
Resource Type:
Accepted Manuscript
Journal Name:
IUCrJ
Additional Journal Information:
Journal Volume: 4; Journal Issue: 6; Journal ID: ISSN 2052-2525
Publisher:
International Union of Crystallography
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; 59 BASIC BIOLOGICAL SCIENCES; drug discovery; protein structure; X-ray crystallography; serial crystallography; time-resolved studies; lysozyme

Citation Formats

Beyerlein, Kenneth R., Dierksmeyer, Dennis, Mariani, Valerio, Kuhn, Manuela, Sarrou, Iosifina, Ottaviano, Angelica, Awel, Salah, Knoska, Juraj, Fuglerud, Silje, Jönsson, Olof, Stern, Stephan, Wiedorn, Max O., Yefanov, Oleksandr, Adriano, Luigi, Bean, Richard, Burkhardt, Anja, Fischer, Pontus, Heymann, Michael, Horke, Daniel A., Jungnickel, Katharina E. J., Kovaleva, Elena, Lorbeer, Olga, Metz, Markus, Meyer, Jan, Morgan, Andrew, Pande, Kanupriya, Panneerselvam, Saravanan, Seuring, Carolin, Tolstikova, Aleksandra, Lieske, Julia, Aplin, Steve, Roessle, Manfred, White, Thomas A., Chapman, Henry N., Meents, Alke, and Oberthuer, Dominik. Mix-and-diffuse serial synchrotron crystallography. United States: N. p., 2017. Web. doi:10.1107/S2052252517013124.
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Beyerlein, Kenneth R., Dierksmeyer, Dennis, Mariani, Valerio, Kuhn, Manuela, Sarrou, Iosifina, Ottaviano, Angelica, Awel, Salah, Knoska, Juraj, Fuglerud, Silje, Jönsson, Olof, Stern, Stephan, Wiedorn, Max O., Yefanov, Oleksandr, Adriano, Luigi, Bean, Richard, Burkhardt, Anja, Fischer, Pontus, Heymann, Michael, Horke, Daniel A., Jungnickel, Katharina E. J., Kovaleva, Elena, Lorbeer, Olga, Metz, Markus, Meyer, Jan, Morgan, Andrew, Pande, Kanupriya, Panneerselvam, Saravanan, Seuring, Carolin, Tolstikova, Aleksandra, Lieske, Julia, Aplin, Steve, Roessle, Manfred, White, Thomas A., Chapman, Henry N., Meents, Alke, & Oberthuer, Dominik. Mix-and-diffuse serial synchrotron crystallography. United States. https://doi.org/10.1107/S2052252517013124
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Beyerlein, Kenneth R., Dierksmeyer, Dennis, Mariani, Valerio, Kuhn, Manuela, Sarrou, Iosifina, Ottaviano, Angelica, Awel, Salah, Knoska, Juraj, Fuglerud, Silje, Jönsson, Olof, Stern, Stephan, Wiedorn, Max O., Yefanov, Oleksandr, Adriano, Luigi, Bean, Richard, Burkhardt, Anja, Fischer, Pontus, Heymann, Michael, Horke, Daniel A., Jungnickel, Katharina E. J., Kovaleva, Elena, Lorbeer, Olga, Metz, Markus, Meyer, Jan, Morgan, Andrew, Pande, Kanupriya, Panneerselvam, Saravanan, Seuring, Carolin, Tolstikova, Aleksandra, Lieske, Julia, Aplin, Steve, Roessle, Manfred, White, Thomas A., Chapman, Henry N., Meents, Alke, and Oberthuer, Dominik. Mon . "Mix-and-diffuse serial synchrotron crystallography". United States. https://doi.org/10.1107/S2052252517013124. https://www.osti.gov/servlets/purl/1410554.
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@article{osti_1410554,
title = {Mix-and-diffuse serial synchrotron crystallography},
author = {Beyerlein, Kenneth R. and Dierksmeyer, Dennis and Mariani, Valerio and Kuhn, Manuela and Sarrou, Iosifina and Ottaviano, Angelica and Awel, Salah and Knoska, Juraj and Fuglerud, Silje and Jönsson, Olof and Stern, Stephan and Wiedorn, Max O. and Yefanov, Oleksandr and Adriano, Luigi and Bean, Richard and Burkhardt, Anja and Fischer, Pontus and Heymann, Michael and Horke, Daniel A. and Jungnickel, Katharina E. J. and Kovaleva, Elena and Lorbeer, Olga and Metz, Markus and Meyer, Jan and Morgan, Andrew and Pande, Kanupriya and Panneerselvam, Saravanan and Seuring, Carolin and Tolstikova, Aleksandra and Lieske, Julia and Aplin, Steve and Roessle, Manfred and White, Thomas A. and Chapman, Henry N. and Meents, Alke and Oberthuer, Dominik},
abstractNote = {Unravelling the interaction of biological macromolecules with ligands and substrates at high spatial and temporal resolution remains a major challenge in structural biology. The development of serial crystallography methods at X-ray free-electron lasers and subsequently at synchrotron light sources allows new approaches to tackle this challenge. Here, a new polyimide tape drive designed for mix-and-diffuse serial crystallography experiments is reported. The structure of lysozyme bound by the competitive inhibitor chitotriose was determined using this device in combination with microfluidic mixers. The electron densities obtained from mixing times of 2 and 50 s show clear binding of chitotriose to the enzyme at a high level of detail. Here, the success of this approach shows the potential for high-throughput drug screening and even structural enzymology on short timescales at bright synchrotron light sources.},
doi = {10.1107/S2052252517013124},
journal = {IUCrJ},
number = 6,
volume = 4,
place = {United States},
year = {2017},
month = {10}
}
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https://doi.org/10.1107/S2052252517013124
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Figures / Tables:

Figure 1 Figure 1: Schematics of the experimental setup. (a) The native protein crystal suspension (yellow) is mixed inline with the substrate solution (blue) before being deposited onto the detector side of the tape. The X-ray beam (red) is focused on the centre of the polyimide tape (yellow ribbon), which is beingmore » drawn from the feeder roll on the left to the collector roll on the right. A diffraction pattern measured in transmission is illustrated as red dots on the grey detector. (b) A technical drawing of the tape-drive device shows how tape is held under tension between two rollers near the interaction region and that X-rays pass through a hole in the centre of the device.« less
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Works referenced in this record:

PHENIX: a comprehensive Python-based system for macromolecular structure solution
journal, January 2010

  • Adams, Paul D.; Afonine, Pavel V.; Bunkóczi, Gábor
  • Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 2, p. 213-221
  • DOI: 10.1107/S0907444909052925

Towards automated crystallographic structure refinement with phenix.refine
journal, March 2012

  • Afonine, Pavel V.; Grosse-Kunstleve, Ralf W.; Echols, Nathaniel
  • Acta Crystallographica Section D Biological Crystallography, Vol. 68, Issue 4
  • DOI: 10.1107/S0907444912001308

Direct observation of ultrafast collective motions in CO myoglobin upon ligand dissociation
journal, September 2015

De novo protein crystal structure determination from X-ray free-electron laser data
journal, November 2013

  • Barends, Thomas R. M.; Foucar, Lutz; Botha, Sabine
  • Nature, Vol. 505, Issue 7482
  • DOI: 10.1038/nature12773

Room-temperature serial crystallography at synchrotron X-ray sources using slowly flowing free-standing high-viscosity microstreams
journal, January 2015

  • Botha, Sabine; Nass, Karol; Barends, Thomas R. M.
  • Acta Crystallographica Section D Biological Crystallography, Vol. 71, Issue 2
  • DOI: 10.1107/S1399004714026327

High-Resolution Protein Structure Determination by Serial Femtosecond Crystallography
journal, May 2012

Status of the crystallography beamlines at PETRA III
journal, March 2016

  • Burkhardt, Anja; Pakendorf, Tim; Reime, Bernd
  • The European Physical Journal Plus, Vol. 131, Issue 3
  • DOI: 10.1140/epjp/i2016-16056-0

Mixing injector enables time-resolved crystallography with high hit rate at X-ray free electron lasers
journal, August 2016

  • Calvey, George D.; Katz, Andrea M.; Schaffer, Chris B.
  • Structural Dynamics, Vol. 3, Issue 5
  • DOI: 10.1063/1.4961971

Femtosecond X-ray protein nanocrystallography
journal, February 2011

  • Chapman, Henry N.; Fromme, Petra; Barty, Anton
  • Nature, Vol. 470, Issue 7332, p. 73-77
  • DOI: 10.1038/nature09750

Refinement of an enzyme complex with inhibitor bound at partial occupancy
journal, April 1992

MolProbity : all-atom structure validation for macromolecular crystallography
journal, December 2009

  • Chen, Vincent B.; Arendall, W. Bryan; Headd, Jeffrey J.
  • Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 1
  • DOI: 10.1107/S0907444909042073

De novo phasing with X-ray laser reveals mosquito larvicide BinAB structure
journal, September 2016

  • Colletier, Jacques-Philippe; Sawaya, Michael R.; Gingery, Mari
  • Nature, Vol. 539, Issue 7627
  • DOI: 10.1038/nature19825

Keynote review: Structural biology and drug discovery
journal, July 2005

Features and development of Coot
journal, March 2010

  • Emsley, P.; Lohkamp, B.; Scott, W. G.
  • Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 4
  • DOI: 10.1107/S0907444910007493

Diffraction-limited storage rings – a window to the science of tomorrow
journal, August 2014

  • Eriksson, Mikael; van der Veen, J. Friso; Quitmann, Christoph
  • Journal of Synchrotron Radiation, Vol. 21, Issue 5
  • DOI: 10.1107/S1600577514019286

Twenty years on: the impact of fragments on drug discovery
journal, July 2016

  • Erlanson, Daniel A.; Fesik, Stephen W.; Hubbard, Roderick E.
  • Nature Reviews Drug Discovery, Vol. 15, Issue 9
  • DOI: 10.1038/nrd.2016.109

Atomic structure of granulin determined from native nanocrystalline granulovirus using an X-ray free-electron laser
journal, February 2017

  • Gati, Cornelius; Oberthuer, Dominik; Yefanov, Oleksandr
  • Proceedings of the National Academy of Sciences, Vol. 114, Issue 9
  • DOI: 10.1073/pnas.1609243114

Crystal Structure of the Mutant D52S Hen Egg White Lysozyme with an Oligosaccharide Product
journal, November 1994

  • Hadfield, A. T.; Harvey, D. J.; Archer, D. B.
  • Journal of Molecular Biology, Vol. 243, Issue 5
  • DOI: 10.1006/jmbi.1994.1688

Catalysis in the crystal: synchrotron radiation studies with glycogen phosphorylase b.
journal, February 1987

The adaptive gain integrating pixel detector AGIPD a detector for the European XFEL
journal, May 2011

  • Henrich, B.; Becker, J.; Dinapoli, R.
  • Nuclear Instruments and Methods in Physics Research Section A: Accelerators, Spectrometers, Detectors and Associated Equipment, Vol. 633
  • DOI: 10.1016/j.nima.2010.06.107

The early history of lysozyme
journal, November 1998

  • Johnson, Louise N.
  • Nature Structural Biology, Vol. 5, Issue 11
  • DOI: 10.1038/2917

Crystal structure of rhodopsin bound to arrestin by femtosecond X-ray laser
journal, July 2015

  • Kang, Yanyong; Zhou, X. Edward; Gao, Xiang
  • Nature, Vol. 523, Issue 7562
  • DOI: 10.1038/nature14656

ValLigURL: a server for ligand-structure comparison and validation
journal, July 2007

  • Kleywegt, Gerard J.; Harris, Mark R.
  • Acta Crystallographica Section D Biological Crystallography, Vol. 63, Issue 8
  • DOI: 10.1107/S090744490703315X

Structural enzymology using X-ray free electron lasers
journal, December 2016

  • Kupitz, Christopher; Olmos, Jose L.; Holl, Mark
  • Structural Dynamics, Vol. 4, Issue 4
  • DOI: 10.1063/1.4972069

PDBsum new things
journal, January 2009

  • Laskowski, R. A.
  • Nucleic Acids Research, Vol. 37, Issue Database
  • DOI: 10.1093/nar/gkn860

LigPlot+: Multiple Ligand–Protein Interaction Diagrams for Drug Discovery
journal, October 2011

  • Laskowski, Roman A.; Swindells, Mark B.
  • Journal of Chemical Information and Modeling, Vol. 51, Issue 10
  • DOI: 10.1021/ci200227u

Serial Femtosecond Crystallography of G Protein-Coupled Receptors
journal, December 2013

Dissection of Protein–Carbohydrate Interactions in Mutant Hen Egg-white Lysozyme Complexes and their Hydrolytic Activity
journal, March 1995

  • Maenaka, Katsumi; Matsushima, Masaaki; Song, Haiwei
  • Journal of Molecular Biology, Vol. 247, Issue 2
  • DOI: 10.1006/jmbi.1994.0139

OnDA : online data analysis and feedback for serial X-ray imaging
journal, May 2016

  • Mariani, Valerio; Morgan, Andrew; Yoon, Chun Hong
  • Journal of Applied Crystallography, Vol. 49, Issue 3
  • DOI: 10.1107/S1600576716007469

Serial millisecond crystallography of membrane and soluble protein microcrystals using synchrotron radiation
journal, May 2017

  • Martin-Garcia, Jose M.; Conrad, Chelsie E.; Nelson, Garrett
  • IUCrJ, Vol. 4, Issue 4, p. 439-454
  • DOI: 10.1107/S205225251700570X

Pink-beam serial crystallography
journal, November 2017

Time-resolved crystallography and protein design: signalling photoreceptors and optogenetics
journal, July 2014

  • Moffat, Keith
  • Philosophical Transactions of the Royal Society B: Biological Sciences, Vol. 369, Issue 1647
  • DOI: 10.1098/rstb.2013.0568

electronic Ligand Builder and Optimization Workbench ( eLBOW ): a tool for ligand coordinate and restraint generation
journal, September 2009

  • Moriarty, Nigel W.; Grosse-Kunstleve, Ralf W.; Adams, Paul D.
  • Acta Crystallographica Section D Biological Crystallography, Vol. 65, Issue 10
  • DOI: 10.1107/S0907444909029436

A three-dimensional movie of structural changes in bacteriorhodopsin
journal, December 2016

  • Nango, Eriko; Royant, Antoine; Kubo, Minoru
  • Science, Vol. 354, Issue 6319, p. 1552-1557
  • DOI: 10.1126/science.aah3497

Time-resolved structural studies at synchrotrons and X-ray free electron lasers: opportunities and challenges
journal, October 2012

Lipidic cubic phase serial millisecond crystallography using synchrotron radiation
journal, March 2015

Double-flow focused liquid injector for efficient serial femtosecond crystallography
journal, March 2017

  • Oberthuer, Dominik; Knoška, Juraj; Wiedorn, Max O.
  • Scientific Reports, Vol. 7, Issue 1
  • DOI: 10.1038/srep44628

Intracellular allosteric antagonism of the CCR9 receptor
journal, December 2016

  • Oswald, Christine; Rappas, Mathieu; Kean, James
  • Nature, Vol. 540, Issue 7633
  • DOI: 10.1038/nature20606

Femtosecond structural dynamics drives the trans/cis isomerization in photoactive yellow protein
journal, May 2016

Advantages of crystallographic fragment screening: Functional and mechanistic insights from a powerful platform for efficient drug discovery
journal, November 2014

The LAMBDA photon-counting pixel detector
journal, March 2013

The use of Nuclear Magnetic Resonance to Describe Relative Modes of Binding to Lysozyme of Homologous Inhibitors and Related Substrates
journal, January 1969

  • Raftery, M. A.; Dahlquist, F. W.; Parsons, S. M.
  • Proceedings of the National Academy of Sciences, Vol. 62, Issue 1
  • DOI: 10.1073/pnas.62.1.44

Calibration status and plans for the charge integrating JUNGFRAU pixel detector for SwissFEL
journal, November 2016

Acoustic Injectors for Drop-On-Demand Serial Femtosecond Crystallography
journal, April 2016

Acoustic methods for high-throughput protein crystal mounting at next-generation macromolecular crystallographic beamlines
journal, August 2013

  • Roessler, Christian G.; Kuczewski, Anthony; Stearns, Richard
  • Journal of Synchrotron Radiation, Vol. 20, Issue 5
  • DOI: 10.1107/S0909049513020372

Six Biophysical Screening Methods Miss a Large Proportion of Crystallographically Discovered Fragment Hits: A Case Study
journal, April 2016

Mix and Inject: Reaction Initiation by Diffusion for Time-Resolved Macromolecular Crystallography
journal, January 2013

Structures of riboswitch RNA reaction states by mix-and-inject XFEL serial crystallography
journal, November 2016

  • Stagno, J. R.; Liu, Y.; Bhandari, Y. R.
  • Nature, Vol. 541, Issue 7636
  • DOI: 10.1038/nature20599

Room-temperature macromolecular serial crystallography using synchrotron radiation
journal, May 2014

Intermediate trapping and laue X-ray diffraction: Potential for enzyme mechanism, dynamics, and inhibitor screening
journal, January 1996

Time-resolved serial crystallography captures high-resolution intermediates of photoactive yellow protein
journal, December 2014

Ligand identification using electron-density map correlations
journal, December 2006

  • Terwilliger, Thomas C.; Adams, Paul D.; Moriarty, Nigel W.
  • Acta Crystallographica Section D Biological Crystallography, Vol. 63, Issue 1
  • DOI: 10.1107/S0907444906046233

Automated ligand fitting by core-fragment fitting and extension into density
journal, July 2006

  • Terwilliger, Thomas C.; Klei, Herbert; Adams, Paul D.
  • Acta Crystallographica Section D Biological Crystallography, Vol. 62, Issue 8
  • DOI: 10.1107/S0907444906017161

Crystallographic model quality at a glance
journal, February 2009

  • Urzhumtseva, Ludmila; Afonine, Pavel V.; Adams, Paul D.
  • Acta Crystallographica Section D Biological Crystallography, Vol. 65, Issue 3
  • DOI: 10.1107/S0907444908044296

Development of neuraminidase inhibitors as anti-influenza virus drugs
journal, March 1999

Catalysis by hen egg-white lysozyme proceeds via a covalent intermediate
journal, August 2001

  • Vocadlo, David J.; Davies, Gideon J.; Laine, Roger
  • Nature, Vol. 412, Issue 6849
  • DOI: 10.1038/35090602

Binding of N -acetylglucosamine oligosaccharides to hen egg-white lysozyme: a powder diffraction study
journal, December 2004

  • Von Dreele, R. B.
  • Acta Crystallographica Section D Biological Crystallography, Vol. 61, Issue 1
  • DOI: 10.1107/S0907444904025715

Post-refinement method for snapshot serial crystallography
journal, July 2014

  • White, Thomas A.
  • Philosophical Transactions of the Royal Society B: Biological Sciences, Vol. 369, Issue 1647
  • DOI: 10.1098/rstb.2013.0330

Crystallographic data processing for free-electron laser sources
journal, June 2013

  • White, Thomas A.; Barty, Anton; Stellato, Francesco
  • Acta Crystallographica Section D Biological Crystallography, Vol. 69, Issue 7
  • DOI: 10.1107/S0907444913013620

CrystFEL : a software suite for snapshot serial crystallography
journal, March 2012

  • White, Thomas A.; Kirian, Richard A.; Martin, Andrew V.
  • Journal of Applied Crystallography, Vol. 45, Issue 2
  • DOI: 10.1107/S0021889812002312

Recent developments in CrystFEL
journal, March 2016

  • White, Thomas A.; Mariani, Valerio; Brehm, Wolfgang
  • Journal of Applied Crystallography, Vol. 49, Issue 2
  • DOI: 10.1107/S1600576716004751

Overview of the CCP 4 suite and current developments
journal, March 2011

  • Winn, Martyn D.; Ballard, Charles C.; Cowtan, Kevin D.
  • Acta Crystallographica Section D Biological Crystallography, Vol. 67, Issue 4
  • DOI: 10.1107/S0907444910045749

An isomorphous replacement method for efficient de novo phasing for serial femtosecond crystallography
journal, September 2015

  • Yamashita, Keitaro; Pan, Dongqing; Okuda, Tomohiko
  • Scientific Reports, Vol. 5, Issue 1
  • DOI: 10.1038/srep14017

Structure of the Angiotensin Receptor Revealed by Serial Femtosecond Crystallography
journal, May 2015

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    • Deutsches Elektronen-Synchrotron, DESY, Hamburg
    • DOI: 10.3204/pubdb-2018-03245

    New insights into the crystallization of polymorphic materials: from real-time serial crystallography to luminescence analysis
    text, January 2019

    • Lindenberg, P.; Arana, L. Ruiz; Mahnke, L. K.
    • Deutsches Elektronen-Synchrotron, DESY, Hamburg
    • DOI: 10.3204/pubdb-2019-03146

    Evaluation of serial crystallographic structure determination within megahertz pulse trains
    text, January 2019

    • Yefanov, Oleksandr; Oberthür, Dominik; Bean, Richard
    • Deutsches Elektronen-Synchrotron, DESY, Hamburg
    • DOI: 10.3204/pubdb-2019-04763

    A fixed-target platform for serial femtosecond crystallography in a hydrated environment
    text, January 2020

    • Shelby, M. L.; Gilbile, D.; Grant, T. D.
    • Deutsches Elektronen-Synchrotron, DESY, Hamburg
    • DOI: 10.3204/pubdb-2019-05316

    Ultracompact 3D microfluidics for time-resolved structural biology
    text, January 2020

    • Knoška, Juraj; Adriano, Luigi; Awel, Salah
    • Deutsches Elektronen-Synchrotron, DESY, Hamburg
    • DOI: 10.3204/pubdb-2020-00687

    Serial protein crystallography in an electron microscope
    text, January 2020

    • Bücker, Robert; Hogan-Lamarre, Pascal; Mehrabi, Pedram
    • Deutsches Elektronen-Synchrotron, DESY, Hamburg
    • DOI: 10.3204/pubdb-2020-00822

    Megahertz serial crystallography
    journal, October 2018

    The hit-and-return system enables efficient time-resolved serial synchrotron crystallography
    journal, October 2018

    • Schulz, Eike C.; Mehrabi, Pedram; Müller-Werkmeister, Henrike M.
    • Nature Methods, Vol. 15, Issue 11
    • DOI: 10.1038/s41592-018-0180-2

    Sample manipulation and data assembly for robust microcrystal synchrotron crystallography
    journal, July 2018

    • Liu, Qun; Guo, Gongrui; Fuchs, Martin R.
    • Acta Crystallographica Section A Foundations and Advances, Vol. 74, Issue a1
    • DOI: 10.1107/s0108767318096046

    Strategies for sample delivery for femtosecond crystallography
    text, January 2019

    Rapid sample delivery for megahertz serial crystallography at X-ray FELs
    text, January 2018

    • Wiedorn, Max Oliver; Awel, Salah; Morgan, Andrew J.
    • TUHH Universitätsbibliothek
    • DOI: 10.15480/882.2221

    On-chip crystallization for serial crystallography experiments and on-chip ligand-binding studies
    text, January 2019

    • Lieske, Julia; Cerv, Maximilian; Kreida, Stefan
    • Deutsches Elektronen-Synchrotron, DESY, Hamburg
    • DOI: 10.3204/pubdb-2019-02620

    High-viscosity injector-based pink-beam serial crystallography of microcrystals at a synchrotron radiation source
    text, January 2019

    Serial protein crystallography in an electron microscope
    text, January 2020

    • Bücker, Robert; Hogan-Lamarre, Pascal; Mehrabi, Pedram
    • Nature Publishing Group UK
    • DOI: 10.15480/882.2722

    Ultracompact 3D microfluidics for time-resolved structural biology
    text, January 2020

    • Knoska, Juraj; Adriano, Luigi; Awel, Salah
    • Deutsches Elektronen-Synchrotron, DESY, Hamburg
    • DOI: 10.3204/pubdb-2020-00623

    Enzyme intermediates captured “on the fly” by mix-and-inject serial crystallography
    journal, May 2018

    Megahertz serial crystallography
    text, January 2018

    • Wiedorn, Max Oliver; Oberthür, Dominik; Bean, Richard
    • Nature Publishing Group UK
    • DOI: 10.15480/882.2175

    De novo protein structure determination by heavy-atom soaking in lipidic cubic phase and SIRAS phasing using serial synchrotron crystallography
    text, January 2018

    • Botha, S.; Baitan, D.; Jungnickel, K. E. J.
    • Deutsches Elektronen-Synchrotron, DESY, Hamburg
    • DOI: 10.3204/pubdb-2018-05499
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