Mix-and-diffuse serial synchrotron crystallography

Beyerlein, Kenneth R.; Dierksmeyer, Dennis; Mariani, Valerio; Kuhn, Manuela; Sarrou, Iosifina; Ottaviano, Angelica; Awel, Salah; Knoska, Juraj; Fuglerud, Silje; Jönsson, Olof; Stern, Stephan; Wiedorn, Max O.; Yefanov, Oleksandr; Adriano, Luigi; Bean, Richard; Burkhardt, Anja; Fischer, Pontus; Heymann, Michael; Horke, Daniel A.; Jungnickel, Katharina E. J.; Kovaleva, Elena; Lorbeer, Olga; Metz, Markus; Meyer, Jan; Morgan, Andrew; Pande, Kanupriya; Panneerselvam, Saravanan; Seuring, Carolin; Tolstikova, Aleksandra; Lieske, Julia; Aplin, Steve; Roessle, Manfred; White, Thomas A.; Chapman, Henry N.; Meents, Alke; Oberthuer, Dominik

doi:10.1107/S2052252517013124

Title: Mix-and-diffuse serial synchrotron crystallography

Journal Article · 09 October 2017 · IUCrJ

DOI: https://doi.org/10.1107/S2052252517013124 · OSTI ID:1410554

^[1]; Dierksmeyer, Dennis ^[1];

^[1]; Kuhn, Manuela ^[1]; Sarrou, Iosifina ^[1];

^[2]; Awel, Salah ^[3];

^[3];

^[4];

^[5]; Stern, Stephan ^[6];

^[3];

^[1]; Adriano, Luigi ^[1];

^[1]; Burkhardt, Anja ^[1]; Fischer, Pontus ^[1];

^[1];

^[3];

^[7] more »

Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany)
California State Univ., Northridge, CA (United States)
Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany); Univ. of Hamburg, Hamburg (Germany)
Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany); Norwegian Univ. of Science and Technology, Trondheim (Norway)
Uppsala Univ., Uppsala (Sweden)
Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany); European X-ray Free-Electron Laser Facility GmbH (XFEL), Schenefeld (Germany)
Univ. of Oxford, Oxford (England)
SLAC National Accelerator Lab., Menlo Park, CA (United States)
Fachhochschule Lubeck, Lubeck (Germany)

Unravelling the interaction of biological macromolecules with ligands and substrates at high spatial and temporal resolution remains a major challenge in structural biology. The development of serial crystallography methods at X-ray free-electron lasers and subsequently at synchrotron light sources allows new approaches to tackle this challenge. Here, a new polyimide tape drive designed for mix-and-diffuse serial crystallography experiments is reported. The structure of lysozyme bound by the competitive inhibitor chitotriose was determined using this device in combination with microfluidic mixers. The electron densities obtained from mixing times of 2 and 50 s show clear binding of chitotriose to the enzyme at a high level of detail. Here, the success of this approach shows the potential for high-throughput drug screening and even structural enzymology on short timescales at bright synchrotron light sources.

View Accepted Manuscript (DOE)

Cite

Export

Save

Research Organization:: SLAC National Accelerator Laboratory (SLAC), Menlo Park, CA (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC02-76SF00515; FP7/2007-2013; ERC-614507-Küpper; DFG-EXC1074

OSTI ID:: 1410554

Journal Information:: IUCrJ, Vol. 4, Issue 6; ISSN 2052-2525

Publisher:: International Union of CrystallographyCopyright Statement

Country of Publication:: United States

Language:: English

Citation Metrics:

Cited by: 85 works

Citation information provided by
Web of Science

References (65)

PHENIX: a comprehensive Python-based system for macromolecular structure solution Adams, Paul D.; Afonine, Pavel V.; Bunkóczi, Gábor Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 2, p. 213-221 https://doi.org/10.1107/S0907444909052925	journal	January 2010
Towards automated crystallographic structure refinement with phenix.refine Afonine, Pavel V.; Grosse-Kunstleve, Ralf W.; Echols, Nathaniel Acta Crystallographica Section D Biological Crystallography, Vol. 68, Issue 4 https://doi.org/10.1107/S0907444912001308	journal	March 2012
Direct observation of ultrafast collective motions in CO myoglobin upon ligand dissociation Barends, T. R. M.; Foucar, L.; Ardevol, A. Science, Vol. 350, Issue 6259 https://doi.org/10.1126/science.aac5492	journal	September 2015
De novo protein crystal structure determination from X-ray free-electron laser data Barends, Thomas R. M.; Foucar, Lutz; Botha, Sabine Nature, Vol. 505, Issue 7482 https://doi.org/10.1038/nature12773	journal	November 2013
Protein crystallography and drug discovery: recollections of knowledge exchange between academia and industry Blundell, Tom L. IUCrJ, Vol. 4, Issue 4 https://doi.org/10.1107/S2052252517009241	journal	June 2017
Room-temperature serial crystallography at synchrotron X-ray sources using slowly flowing free-standing high-viscosity microstreams Botha, Sabine; Nass, Karol; Barends, Thomas R. M. Acta Crystallographica Section D Biological Crystallography, Vol. 71, Issue 2 https://doi.org/10.1107/S1399004714026327	journal	January 2015
High-Resolution Protein Structure Determination by Serial Femtosecond Crystallography Boutet, S.; Lomb, L.; Williams, G. J. Science, Vol. 337, Issue 6092 https://doi.org/10.1126/science.1217737	journal	May 2012
Status of the crystallography beamlines at PETRA III Burkhardt, Anja; Pakendorf, Tim; Reime, Bernd The European Physical Journal Plus, Vol. 131, Issue 3 https://doi.org/10.1140/epjp/i2016-16056-0	journal	March 2016
Mixing injector enables time-resolved crystallography with high hit rate at X-ray free electron lasers Calvey, George D.; Katz, Andrea M.; Schaffer, Chris B. Structural Dynamics, Vol. 3, Issue 5 https://doi.org/10.1063/1.4961971	journal	August 2016
Femtosecond X-ray protein nanocrystallography Chapman, Henry N.; Fromme, Petra; Barty, Anton Nature, Vol. 470, Issue 7332, p. 73-77 https://doi.org/10.1038/nature09750	journal	February 2011
MolProbity : all-atom structure validation for macromolecular crystallography Chen, Vincent B.; Arendall, W. Bryan; Headd, Jeffrey J. Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 1 https://doi.org/10.1107/S0907444909042073	journal	December 2009
De novo phasing with X-ray laser reveals mosquito larvicide BinAB structure Colletier, Jacques-Philippe; Sawaya, Michael R.; Gingery, Mari Nature, Vol. 539, Issue 7627 https://doi.org/10.1038/nature19825	journal	September 2016
Keynote review: Structural biology and drug discovery Congreve, Miles; Murray, Christopher W.; Blundell, Tom L. Drug Discovery Today, Vol. 10, Issue 13 https://doi.org/10.1016/S1359-6446(05)03484-7	journal	July 2005
Features and development of Coot Emsley, P.; Lohkamp, B.; Scott, W. G. Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 4 https://doi.org/10.1107/S0907444910007493	journal	March 2010
Diffraction-limited storage rings – a window to the science of tomorrow Eriksson, Mikael; van der Veen, J. Friso; Quitmann, Christoph Journal of Synchrotron Radiation, Vol. 21, Issue 5 https://doi.org/10.1107/S1600577514019286	journal	August 2014
Twenty years on: the impact of fragments on drug discovery Erlanson, Daniel A.; Fesik, Stephen W.; Hubbard, Roderick E. Nature Reviews Drug Discovery, Vol. 15, Issue 9 https://doi.org/10.1038/nrd.2016.109	journal	July 2016
Atomic structure of granulin determined from native nanocrystalline granulovirus using an X-ray free-electron laser Gati, Cornelius; Oberthuer, Dominik; Yefanov, Oleksandr Proceedings of the National Academy of Sciences, Vol. 114, Issue 9 https://doi.org/10.1073/pnas.1609243114	journal	February 2017
Diffusion ordered spectroscopy as a complement to size exclusion chromatography in oligosaccharide analysis Groves, P. Glycobiology, Vol. 14, Issue 5 https://doi.org/10.1093/glycob/cwh037	journal	January 2004
Crystal Structure of the Mutant D52S Hen Egg White Lysozyme with an Oligosaccharide Product Hadfield, A. T.; Harvey, D. J.; Archer, D. B. Journal of Molecular Biology, Vol. 243, Issue 5 https://doi.org/10.1006/jmbi.1994.1688	journal	November 1994
Catalysis in the crystal: synchrotron radiation studies with glycogen phosphorylase b. Hajdu, J.; Acharya, K. R.; Stuart, D. I. The EMBO Journal, Vol. 6, Issue 2 https://doi.org/10.1002/j.1460-2075.1987.tb04786.x	journal	February 1987
The adaptive gain integrating pixel detector AGIPD a detector for the European XFEL Henrich, B.; Becker, J.; Dinapoli, R. Nuclear Instruments and Methods in Physics Research Section A: Accelerators, Spectrometers, Detectors and Associated Equipment, Vol. 633 https://doi.org/10.1016/j.nima.2010.06.107	journal	May 2011
The early history of lysozyme Johnson, Louise N. Nature Structural Biology, Vol. 5, Issue 11 https://doi.org/10.1038/2917	journal	November 1998
Crystal structure of rhodopsin bound to arrestin by femtosecond X-ray laser Kang, Yanyong; Zhou, X. Edward; Gao, Xiang Nature, Vol. 523, Issue 7562 https://doi.org/10.1038/nature14656	journal	July 2015
ValLigURL: a server for ligand-structure comparison and validation Kleywegt, Gerard J.; Harris, Mark R. Acta Crystallographica Section D Biological Crystallography, Vol. 63, Issue 8 https://doi.org/10.1107/S090744490703315X	journal	July 2007
Structural enzymology using X-ray free electron lasers Kupitz, Christopher; Olmos, Jose L.; Holl, Mark Structural Dynamics, Vol. 4, Issue 4 https://doi.org/10.1063/1.4972069	journal	December 2016
PDBsum new things Laskowski, R. A. Nucleic Acids Research, Vol. 37, Issue Database https://doi.org/10.1093/nar/gkn860	journal	January 2009
LigPlot+: Multiple Ligand–Protein Interaction Diagrams for Drug Discovery Laskowski, Roman A.; Swindells, Mark B. Journal of Chemical Information and Modeling, Vol. 51, Issue 10 https://doi.org/10.1021/ci200227u	journal	October 2011
Serial Femtosecond Crystallography of G Protein-Coupled Receptors Liu, W.; Wacker, D.; Gati, C. Science, Vol. 342, Issue 6165 https://doi.org/10.1126/science.1244142	journal	December 2013
Dissection of Protein–Carbohydrate Interactions in Mutant Hen Egg-white Lysozyme Complexes and their Hydrolytic Activity Maenaka, Katsumi; Matsushima, Masaaki; Song, Haiwei Journal of Molecular Biology, Vol. 247, Issue 2 https://doi.org/10.1006/jmbi.1994.0139	journal	March 1995
OnDA : online data analysis and feedback for serial X-ray imaging Mariani, Valerio; Morgan, Andrew; Yoon, Chun Hong Journal of Applied Crystallography, Vol. 49, Issue 3 https://doi.org/10.1107/S1600576716007469	journal	May 2016
Serial millisecond crystallography of membrane and soluble protein microcrystals using synchrotron radiation Martin-Garcia, Jose M.; Conrad, Chelsie E.; Nelson, Garrett IUCrJ, Vol. 4, Issue 4, p. 439-454 https://doi.org/10.1107/S205225251700570X	journal	May 2017
Pink-beam serial crystallography Meents, A.; Wiedorn, M. O.; Srajer, V. Nature Communications, Vol. 8, Issue 1 https://doi.org/10.1038/s41467-017-01417-3	journal	November 2017
Time-resolved crystallography and protein design: signalling photoreceptors and optogenetics Moffat, Keith Philosophical Transactions of the Royal Society B: Biological Sciences, Vol. 369, Issue 1647 https://doi.org/10.1098/rstb.2013.0568	journal	July 2014
electronic Ligand Builder and Optimization Workbench ( eLBOW ): a tool for ligand coordinate and restraint generation Moriarty, Nigel W.; Grosse-Kunstleve, Ralf W.; Adams, Paul D. Acta Crystallographica Section D Biological Crystallography, Vol. 65, Issue 10 https://doi.org/10.1107/S0907444909029436	journal	September 2009
A three-dimensional movie of structural changes in bacteriorhodopsin Nango, Eriko; Royant, Antoine; Kubo, Minoru Science, Vol. 354, Issue 6319, p. 1552-1557 https://doi.org/10.1126/science.aah3497	journal	December 2016
Time-resolved structural studies at synchrotrons and X-ray free electron lasers: opportunities and challenges Neutze, Richard; Moffat, Keith Current Opinion in Structural Biology, Vol. 22, Issue 5 https://doi.org/10.1016/j.sbi.2012.08.006	journal	October 2012
Lipidic cubic phase serial millisecond crystallography using synchrotron radiation Nogly, Przemyslaw; James, Daniel; Wang, Dingjie IUCrJ, Vol. 2, Issue 2, p. 168-176 https://doi.org/10.1107/S2052252514026487	journal	March 2015
Double-flow focused liquid injector for efficient serial femtosecond crystallography Oberthuer, Dominik; Knoška, Juraj; Wiedorn, Max O. Scientific Reports, Vol. 7, Issue 1 https://doi.org/10.1038/srep44628	journal	March 2017
Intracellular allosteric antagonism of the CCR9 receptor Oswald, Christine; Rappas, Mathieu; Kean, James Nature, Vol. 540, Issue 7633 https://doi.org/10.1038/nature20606	journal	December 2016
Femtosecond structural dynamics drives the trans/cis isomerization in photoactive yellow protein Pande, K.; Hutchison, C. D. M.; Groenhof, G. Science, Vol. 352, Issue 6286 https://doi.org/10.1126/science.aad5081	journal	May 2016
Advantages of crystallographic fragment screening: Functional and mechanistic insights from a powerful platform for efficient drug discovery Patel, Disha; Bauman, Joseph D.; Arnold, Eddy Progress in Biophysics and Molecular Biology, Vol. 116, Issue 2-3 https://doi.org/10.1016/j.pbiomolbio.2014.08.004	journal	November 2014
The LAMBDA photon-counting pixel detector Pennicard, D.; Lange, S.; Smoljanin, S. Journal of Physics: Conference Series, Vol. 425, Issue 6 https://doi.org/10.1088/1742-6596/425/6/062010	journal	March 2013
The use of Nuclear Magnetic Resonance to Describe Relative Modes of Binding to Lysozyme of Homologous Inhibitors and Related Substrates Raftery, M. A.; Dahlquist, F. W.; Parsons, S. M. Proceedings of the National Academy of Sciences, Vol. 62, Issue 1 https://doi.org/10.1073/pnas.62.1.44	journal	January 1969
Calibration status and plans for the charge integrating JUNGFRAU pixel detector for SwissFEL Redford, S.; Bergamaschi, A.; Brückner, M. Journal of Instrumentation, Vol. 11, Issue 11 https://doi.org/10.1088/1748-0221/11/11/C11013	journal	November 2016
Acoustic Injectors for Drop-On-Demand Serial Femtosecond Crystallography Roessler, Christian G.; Agarwal, Rakhi; Allaire, Marc Structure, Vol. 24, Issue 4 https://doi.org/10.1016/j.str.2016.02.007	journal	April 2016
Acoustic methods for high-throughput protein crystal mounting at next-generation macromolecular crystallographic beamlines Roessler, Christian G.; Kuczewski, Anthony; Stearns, Richard Journal of Synchrotron Radiation, Vol. 20, Issue 5 https://doi.org/10.1107/S0909049513020372	journal	August 2013
Six Biophysical Screening Methods Miss a Large Proportion of Crystallographically Discovered Fragment Hits: A Case Study Schiebel, Johannes; Radeva, Nedyalka; Krimmer, Stefan G. ACS Chemical Biology, Vol. 11, Issue 6 https://doi.org/10.1021/acschembio.5b01034	journal	April 2016
Mix and Inject: Reaction Initiation by Diffusion for Time-Resolved Macromolecular Crystallography Schmidt, Marius Advances in Condensed Matter Physics, Vol. 2013 https://doi.org/10.1155/2013/167276	journal	January 2013
Structures of riboswitch RNA reaction states by mix-and-inject XFEL serial crystallography Stagno, J. R.; Liu, Y.; Bhandari, Y. R. Nature, Vol. 541, Issue 7636 https://doi.org/10.1038/nature20599	journal	November 2016
Room-temperature macromolecular serial crystallography using synchrotron radiation Stellato, Francesco; Oberthür, Dominik; Liang, Mengning IUCrJ, Vol. 1, Issue 4, p. 204-212 https://doi.org/10.1107/S2052252514010070	journal	May 2014
Intermediate trapping and laue X-ray diffraction: Potential for enzyme mechanism, dynamics, and inhibitor screening Stoddard, Barry L. Pharmacology & Therapeutics, Vol. 70, Issue 3 https://doi.org/10.1016/0163-7258(96)00058-7	journal	January 1996
Time-resolved serial crystallography captures high-resolution intermediates of photoactive yellow protein Tenboer, Jason; Basu, Shibom; Zatsepin, Nadia Science, Vol. 346, Issue 6214 https://doi.org/10.1126/science.1259357	journal	December 2014
Ligand identification using electron-density map correlations Terwilliger, Thomas C.; Adams, Paul D.; Moriarty, Nigel W. Acta Crystallographica Section D Biological Crystallography, Vol. 63, Issue 1 https://doi.org/10.1107/S0907444906046233	journal	December 2006
Automated ligand fitting by core-fragment fitting and extension into density Terwilliger, Thomas C.; Klei, Herbert; Adams, Paul D. Acta Crystallographica Section D Biological Crystallography, Vol. 62, Issue 8 https://doi.org/10.1107/S0907444906017161	journal	July 2006
Crystallographic model quality at a glance Urzhumtseva, Ludmila; Afonine, Pavel V.; Adams, Paul D. Acta Crystallographica Section D Biological Crystallography, Vol. 65, Issue 3 https://doi.org/10.1107/S0907444908044296	journal	February 2009
Development of neuraminidase inhibitors as anti-influenza virus drugs Varghese, Joseph N. Drug Development Research, Vol. 46, Issue 3-4 https://doi.org/10.1002/(SICI)1098-2299(199903/04)46:3/4<176::AID-DDR4>3.0.CO;2-6	journal	March 1999
Catalysis by hen egg-white lysozyme proceeds via a covalent intermediate Vocadlo, David J.; Davies, Gideon J.; Laine, Roger Nature, Vol. 412, Issue 6849 https://doi.org/10.1038/35090602	journal	August 2001
Binding of N -acetylglucosamine oligosaccharides to hen egg-white lysozyme: a powder diffraction study Von Dreele, R. B. Acta Crystallographica Section D Biological Crystallography, Vol. 61, Issue 1 https://doi.org/10.1107/S0907444904025715	journal	December 2004
Post-refinement method for snapshot serial crystallography White, Thomas A. Philosophical Transactions of the Royal Society B: Biological Sciences, Vol. 369, Issue 1647 https://doi.org/10.1098/rstb.2013.0330	journal	July 2014
Crystallographic data processing for free-electron laser sources White, Thomas A.; Barty, Anton; Stellato, Francesco Acta Crystallographica Section D Biological Crystallography, Vol. 69, Issue 7 https://doi.org/10.1107/S0907444913013620	journal	June 2013
CrystFEL : a software suite for snapshot serial crystallography White, Thomas A.; Kirian, Richard A.; Martin, Andrew V. Journal of Applied Crystallography, Vol. 45, Issue 2 https://doi.org/10.1107/S0021889812002312	journal	March 2012
Recent developments in CrystFEL White, Thomas A.; Mariani, Valerio; Brehm, Wolfgang Journal of Applied Crystallography, Vol. 49, Issue 2 https://doi.org/10.1107/S1600576716004751	journal	March 2016
Overview of the CCP 4 suite and current developments Winn, Martyn D.; Ballard, Charles C.; Cowtan, Kevin D. Acta Crystallographica Section D Biological Crystallography, Vol. 67, Issue 4 https://doi.org/10.1107/S0907444910045749	journal	March 2011
An isomorphous replacement method for efficient de novo phasing for serial femtosecond crystallography Yamashita, Keitaro; Pan, Dongqing; Okuda, Tomohiko Scientific Reports, Vol. 5, Issue 1 https://doi.org/10.1038/srep14017	journal	September 2015
Structure of the Angiotensin Receptor Revealed by Serial Femtosecond Crystallography Zhang, Haitao; Unal, Hamiyet; Gati, Cornelius Cell, Vol. 161, Issue 4 https://doi.org/10.1016/j.cell.2015.04.011	journal	May 2015

Cited By (39)

Ultracompact 3D microfluidics for time-resolved structural biology Knoška, Juraj; Adriano, Luigi; Awel, Salah Nature Communications, Vol. 11, Issue 1 https://doi.org/10.1038/s41467-020-14434-6	journal	January 2020
Serial protein crystallography in an electron microscope Bücker, Robert; Hogan-Lamarre, Pascal; Mehrabi, Pedram Nature Communications, Vol. 11, Issue 1 https://doi.org/10.1038/s41467-020-14793-0	journal	February 2020
Fast and accurate data collection for macromolecular crystallography using the JUNGFRAU detector Leonarski, Filip; Redford, Sophie; Mozzanica, Aldo Nature Methods, Vol. 15, Issue 10 https://doi.org/10.1038/s41592-018-0143-7	journal	October 2018
Liquid application method for time-resolved analyses by serial synchrotron crystallography Mehrabi, Pedram; Schulz, Eike C.; Agthe, Michael Nature Methods, Vol. 16, Issue 10 https://doi.org/10.1038/s41592-019-0553-1	journal	September 2019
New insights into the crystallization of polymorphic materials: from real-time serial crystallography to luminescence analysis Lindenberg, P.; Arana, L. Ruiz; Mahnke, L. K. Reaction Chemistry & Engineering, Vol. 4, Issue 10 https://doi.org/10.1039/c9re00191c	journal	January 2019
3D printed nozzles on a silicon fluidic chip Bohne, Sven; Heymann, Michael; Chapman, Henry N. Review of Scientific Instruments, Vol. 90, Issue 3 https://doi.org/10.1063/1.5080428	journal	March 2019
Evaluation of serial crystallographic structure determination within megahertz pulse trains Yefanov, Oleksandr; Oberthür, Dominik; Bean, Richard Structural Dynamics, Vol. 6, Issue 6 https://doi.org/10.1063/1.5124387	journal	November 2019
PETRA IV: the ultralow-emittance source project at DESY Schroer, Christian G.; Agapov, Ilya; Brefeld, Werner Journal of Synchrotron Radiation, Vol. 25, Issue 5 https://doi.org/10.1107/s1600577518008858	journal	August 2018
De novo protein structure determination by heavy-atom soaking in lipidic cubic phase and SIRAS phasing using serial synchrotron crystallography Botha, S.; Baitan, D.; Jungnickel, K. E. J. IUCrJ, Vol. 5, Issue 5 https://doi.org/10.1107/s2052252518009223	journal	August 2018
High-viscosity injector-based pink-beam serial crystallography of microcrystals at a synchrotron radiation source Martin-Garcia, Jose M.; Zhu, Lan; Mendez, Derek IUCrJ, Vol. 6, Issue 3 https://doi.org/10.1107/s205225251900263x	journal	April 2019
On-chip crystallization for serial crystallography experiments and on-chip ligand-binding studies Lieske, Julia; Cerv, Maximilian; Kreida, Stefan IUCrJ, Vol. 6, Issue 4 https://doi.org/10.1107/s2052252519007395	journal	June 2019
A fixed-target platform for serial femtosecond crystallography in a hydrated environment Shelby, M. L.; Gilbile, D.; Grant, T. D. IUCrJ, Vol. 7, Issue 1 https://doi.org/10.1107/s2052252519014003	journal	January 2020
Sample delivery for serial crystallography at free-electron lasers and synchrotrons Grünbein, Marie Luise; Nass Kovacs, Gabriela Acta Crystallographica Section D Structural Biology, Vol. 75, Issue 2 https://doi.org/10.1107/s205979831801567x	journal	January 2019
A guide to sample delivery systems for serial crystallography Zhao, Feng‐Zhu; Zhang, Bin; Yan, Er‐Kai The FEBS Journal, Vol. 286, Issue 22 https://doi.org/10.1111/febs.15099	journal	August 2019
Rapid Sample Delivery for Megahertz Serial Crystallography at X-ray FELs Wiedorn, Max O.; Awel, Salah; Morgan, Andrew J. Deutsches Elektronen-Synchrotron, DESY, Hamburg https://doi.org/10.3204/pubdb-2018-02209	text	January 2018
Megahertz serial crystallography Wiedorn, Max O.; Oberthür, Dominik; Bean, Richard Deutsches Elektronen-Synchrotron, DESY, Hamburg https://doi.org/10.3204/pubdb-2018-03705	text	January 2018
The hit-and-return system enables efficient time-resolved serial synchrotron crystallography Schulz, Eike C.; Mehrabi, Pedram; Müller-Werkmeister, Henrike M. Deutsches Elektronen-Synchrotron, DESY, Hamburg https://doi.org/10.3204/pubdb-2019-00026	text	January 2018
A microfluidic flow-focusing device for low sample consumption serial synchrotron crystallography experiments in liquid flow Monteiro, Diana C. F.; Vakili, Mohammad; Harich, Jessica International Union of Crystallography (IUCr) https://doi.org/10.1107/s1600577519000304/rv5100sup1.pdf	text	January 2019
Rapid sample delivery for megahertz serial crystallography at X-ray FELs Wiedorn, Max O.; Awel, Salah; Morgan, Andrew J. IUCrJ, Vol. 5, Issue 5 https://doi.org/10.1107/S2052252518008369	journal	July 2018
On-chip crystallization for serial crystallography experiments and on-chip ligand-binding studies Lieske, Julia; Cerv, Maximilian; Kreida, Stefan International Union of Crystallography (IUCr) https://doi.org/10.1107/s2052252519007395/mf5034sup1.pdf	text	January 2019
3D printed nozzles on a silicon fluidic chip Bohne, Sven; Heymann, Michael; Chapman, Henry N. American Institute of Physics https://doi.org/10.15480/882.2171	text	January 2019
PETRA IV: the ultralow-emittance source project at DESY Schroer, Christian G.; Agapov, Ilya; Brefeld, Werner Deutsches Elektronen-Synchrotron, DESY, Hamburg https://doi.org/10.3204/pubdb-2018-03245	text	January 2018
New insights into the crystallization of polymorphic materials: from real-time serial crystallography to luminescence analysis Lindenberg, P.; Arana, L. Ruiz; Mahnke, L. K. Deutsches Elektronen-Synchrotron, DESY, Hamburg https://doi.org/10.3204/pubdb-2019-03146	text	January 2019
Evaluation of serial crystallographic structure determination within megahertz pulse trains Yefanov, Oleksandr; Oberthür, Dominik; Bean, Richard Deutsches Elektronen-Synchrotron, DESY, Hamburg https://doi.org/10.3204/pubdb-2019-04763	text	January 2019
A fixed-target platform for serial femtosecond crystallography in a hydrated environment Shelby, M. L.; Gilbile, D.; Grant, T. D. Deutsches Elektronen-Synchrotron, DESY, Hamburg https://doi.org/10.3204/pubdb-2019-05316	text	January 2020
Ultracompact 3D microfluidics for time-resolved structural biology Knoška, Juraj; Adriano, Luigi; Awel, Salah Deutsches Elektronen-Synchrotron, DESY, Hamburg https://doi.org/10.3204/pubdb-2020-00687	text	January 2020
Serial protein crystallography in an electron microscope Bücker, Robert; Hogan-Lamarre, Pascal; Mehrabi, Pedram Deutsches Elektronen-Synchrotron, DESY, Hamburg https://doi.org/10.3204/pubdb-2020-00822	text	January 2020
Megahertz serial crystallography Wiedorn, Max O.; Oberthür, Dominik; Bean, Richard Nature Communications, Vol. 9, Issue 1 https://doi.org/10.1038/s41467-018-06156-7	journal	October 2018
The hit-and-return system enables efficient time-resolved serial synchrotron crystallography Schulz, Eike C.; Mehrabi, Pedram; Müller-Werkmeister, Henrike M. Nature Methods, Vol. 15, Issue 11 https://doi.org/10.1038/s41592-018-0180-2	journal	October 2018
Sample manipulation and data assembly for robust microcrystal synchrotron crystallography Liu, Qun; Guo, Gongrui; Fuchs, Martin R. Acta Crystallographica Section A Foundations and Advances, Vol. 74, Issue a1 https://doi.org/10.1107/s0108767318096046	journal	July 2018
Strategies for sample delivery for femtosecond crystallography Martiel, Isabelle; Müller-Werkmeister, Henrike M.; Cohen, Aina E. International Union of Crystallography (IUCr) https://doi.org/10.1107/s2059798318017953/ba5296sup1.pdf	text	January 2019
Rapid sample delivery for megahertz serial crystallography at X-ray FELs Wiedorn, Max Oliver; Awel, Salah; Morgan, Andrew J. TUHH Universitätsbibliothek https://doi.org/10.15480/882.2221	text	January 2018
On-chip crystallization for serial crystallography experiments and on-chip ligand-binding studies Lieske, Julia; Cerv, Maximilian; Kreida, Stefan Deutsches Elektronen-Synchrotron, DESY, Hamburg https://doi.org/10.3204/pubdb-2019-02620	text	January 2019
High-viscosity injector-based pink-beam serial crystallography of microcrystals at a synchrotron radiation source Martin-Garcia, Jose M.; Zhu, Lan; Mendez, Derek International Union of Crystallography (IUCr) https://doi.org/10.1107/s205225251900263x/ti5014sup1.pdf	text	January 2019
Serial protein crystallography in an electron microscope Bücker, Robert; Hogan-Lamarre, Pascal; Mehrabi, Pedram Nature Publishing Group UK https://doi.org/10.15480/882.2722	text	January 2020
Ultracompact 3D microfluidics for time-resolved structural biology Knoska, Juraj; Adriano, Luigi; Awel, Salah Deutsches Elektronen-Synchrotron, DESY, Hamburg https://doi.org/10.3204/pubdb-2020-00623	text	January 2020
Enzyme intermediates captured “on the fly” by mix-and-inject serial crystallography Olmos, Jose L.; Pandey, Suraj; Martin-Garcia, Jose M. BMC Biology, Vol. 16, Issue 1 https://doi.org/10.1186/s12915-018-0524-5	journal	May 2018
Megahertz serial crystallography Wiedorn, Max Oliver; Oberthür, Dominik; Bean, Richard Nature Publishing Group UK https://doi.org/10.15480/882.2175	text	January 2018
De novo protein structure determination by heavy-atom soaking in lipidic cubic phase and SIRAS phasing using serial synchrotron crystallography Botha, S.; Baitan, D.; Jungnickel, K. E. J. Deutsches Elektronen-Synchrotron, DESY, Hamburg https://doi.org/10.3204/pubdb-2018-05499	text	January 2018

Figures / Tables (5)

Similar Records

A user-friendly plug-and-play cyclic olefin copolymer-based microfluidic chip for room-temperature, fixed-target serial crystallography

Journal Article · 2023 · Acta Crystallographica. Section D. Structural Biology · OSTI ID:2008115

Liu, Zhongrui; Gu, Kevin K.; Shelby, Megan L.; +14 more

Serial millisecond crystallography of membrane and soluble protein microcrystals using synchrotron radiation

Journal Article · 2017 · IUCrJ · OSTI ID:1374440

Martin-Garcia, Jose M.; Conrad, Chelsie E.; Nelson, Garrett; +27 more

Plug-and-play polymer microfluidic chips for hydrated, room temperature, fixed-target serial crystallography

Journal Article · 2021 · Lab on a Chip · OSTI ID:1884644

Gilbile, Deepshika; Shelby, Megan L.; Lyubimov, Artem Y.; +7 more

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
59 BASIC BIOLOGICAL SCIENCES
drug discovery
protein structure
X-ray crystallography
serial crystallography
time-resolved studies
lysozyme

Title: Mix-and-diffuse serial synchrotron crystallography

Citation Formats

References (65)

Cited By (39)

Figures / Tables (5)

Similar Records

Related Subjects