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Title: Carboxysomes: metabolic modules for CO 2 fixation

Abstract

The carboxysome is a bacterial microcompartment encapsulating the enzymes carbonic anhydrase and ribulose-1,5-bisphosphate carboxylase/oxygenase. As the site of CO 2 fixation, it serves an essential role in the carbon dioxide concentrating mechanism of many chemoautotrophs and all cyanobacteria. Carboxysomes and other bacterial microcompartments self-assemble through specific protein–protein interactions that are typically mediated by conserved protein domains. In this review, we frame our current understanding of carboxysomes in the context of their component protein domains with their inherent function as the ‘building blocks’ of carboxysomes. These building blocks are organized in genetic modules (conserved chromosomal loci) that encode for carboxysomes and ancillary proteins essential for the integration of the organelle with the rest of cellular metabolism. This conceptual framework provides the foundation for ‘plug-and-play’ engineering of carboxysomes as CO 2 fixation modules in a variety of biotechnological applications.

Authors:
 [1];  [2];  [3]
  1. Michigan State Univ., East Lansing, MI (United States). MSU-DOE Plant Research Laboratory
  2. Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
  3. Michigan State Univ., East Lansing, MI (United States). MSU-DOE Plant Research Laboratory and Department of Biochemistry and Molecular Biology; Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Molecular Biophysics and Integrated Bioimaging Division; Berkeley Synthetic Biology Institute, Berkeley, CA (United States)
Publication Date:
Research Org.:
Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
Sponsoring Org.:
USDOE Office of Energy Efficiency and Renewable Energy (EERE); USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
OSTI Identifier:
1409782
Report Number(s):
LA-UR-17-25514
Journal ID: ISSN 1574-6968
Grant/Contract Number:  
AC52-06NA25396; FG02-91ER20021; E0007089
Resource Type:
Accepted Manuscript
Journal Name:
FEMS Microbiology Letters (Online)
Additional Journal Information:
Journal Name: FEMS Microbiology Letters (Online); Journal Volume: 364; Journal Issue: 18; Journal ID: ISSN 1574-6968
Publisher:
Federation of European Microbiological Societies
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; Biological Science

Citation Formats

Turmo, Aiko, Gonzalez-Esquer, Cesar Raul, and Kerfeld, Cheryl A. Carboxysomes: metabolic modules for CO2 fixation. United States: N. p., 2017. Web. doi:10.1093/femsle/fnx176.
Turmo, Aiko, Gonzalez-Esquer, Cesar Raul, & Kerfeld, Cheryl A. Carboxysomes: metabolic modules for CO2 fixation. United States. doi:10.1093/femsle/fnx176.
Turmo, Aiko, Gonzalez-Esquer, Cesar Raul, and Kerfeld, Cheryl A. Mon . "Carboxysomes: metabolic modules for CO2 fixation". United States. doi:10.1093/femsle/fnx176. https://www.osti.gov/servlets/purl/1409782.
@article{osti_1409782,
title = {Carboxysomes: metabolic modules for CO2 fixation},
author = {Turmo, Aiko and Gonzalez-Esquer, Cesar Raul and Kerfeld, Cheryl A.},
abstractNote = {The carboxysome is a bacterial microcompartment encapsulating the enzymes carbonic anhydrase and ribulose-1,5-bisphosphate carboxylase/oxygenase. As the site of CO2 fixation, it serves an essential role in the carbon dioxide concentrating mechanism of many chemoautotrophs and all cyanobacteria. Carboxysomes and other bacterial microcompartments self-assemble through specific protein–protein interactions that are typically mediated by conserved protein domains. In this review, we frame our current understanding of carboxysomes in the context of their component protein domains with their inherent function as the ‘building blocks’ of carboxysomes. These building blocks are organized in genetic modules (conserved chromosomal loci) that encode for carboxysomes and ancillary proteins essential for the integration of the organelle with the rest of cellular metabolism. This conceptual framework provides the foundation for ‘plug-and-play’ engineering of carboxysomes as CO2 fixation modules in a variety of biotechnological applications.},
doi = {10.1093/femsle/fnx176},
journal = {FEMS Microbiology Letters (Online)},
number = 18,
volume = 364,
place = {United States},
year = {2017},
month = {8}
}

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Cited by: 10 works
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