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Title: Correlative cellular ptychography with functionalized nanoparticles at the Fe L-edge

Precise localization of nanoparticles within a cell is crucial to the understanding of cell-particle interactions and has broad applications in nanomedicine. Here in this paper, we report a proof-of-principle experiment for imaging individual functionalized nanoparticles within a mammalian cell by correlative microscopy. Using a chemically-fixed HeLa cell labeled with fluorescent core-shell nanoparticles as a model system, we implemented a graphene-oxide layer as a substrate to significantly reduce background scattering. We identified cellular features of interest by fluorescence microscopy, followed by scanning transmission X-ray tomography to localize the particles in 3D, and ptychographic coherent diffractive imaging of the fine features in the region at high resolution. By tuning the X-ray energy to the Fe L-edge, we demonstrated sensitive detection of nanoparticles composed of a 22 nm magnetic Fe 3O 4 core encased by a 25-nm-thick fluorescent silica (SiO 2) shell. These fluorescent core-shell nanoparticles act as landmarks and offer clarity in a cellular context. Our correlative microscopy results confirmed a subset of particles to be fully internalized, and high-contrast ptychographic images showed two oxidation states of individual nanoparticles with a resolution of ~16.5 nm. The ability to precisely localize individual fluorescent nanoparticles within mammalian cells will expand our understanding of themore » structure/function relationships for functionalized nanoparticles.« less
Authors:
 [1] ;  [1] ;  [1] ;  [2] ;  [3] ;  [1] ;  [2] ; ORCiD logo [4] ;  [5] ;  [1]
  1. Univ. of California, Los Angeles, CA (United States). Dept. of Physics and Astronomy and California NanoSystems Inst.
  2. Brazilian Center for in Energy and Materials (CEP), Sao Paulo (Brazil). Brazilian Synchrotron Lab. (LNLS)
  3. Univ. of California, Los Angeles, CA (United States). Dept. of Physics and Astronomy and California NanoSystems Inst.; Shanghai Jiao Tong Univ. (China). Dept. of Physics and Astronomy
  4. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Advanced Light Source (ALS)
  5. Univ. of California, Los Angeles, CA (United States). Inst. for Genomics and Proteomics, Dept. of Chemistry and Biochemistry
Publication Date:
Grant/Contract Number:
AC02-05CH11231; DMR 1548924; SC0010378
Type:
Accepted Manuscript
Journal Name:
Scientific Reports
Additional Journal Information:
Journal Volume: 7; Journal Issue: 1; Journal ID: ISSN 2045-2322
Publisher:
Nature Publishing Group
Research Org:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
Country of Publication:
United States
Language:
English
Subject:
77 NANOSCIENCE AND NANOTECHNOLOGY; 72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS
OSTI Identifier:
1408491

Gallagher-Jones, Marcus, Dias, Carlos Sato Baraldi, Pryor, Alan, Bouchmella, Karim, Zhao, Lingrong, Lo, Yuan Hung, Cardoso, Mateus Borba, Shapiro, David, Rodriguez, Jose, and Miao, Jianwei. Correlative cellular ptychography with functionalized nanoparticles at the Fe L-edge. United States: N. p., Web. doi:10.1038/s41598-017-04784-5.
Gallagher-Jones, Marcus, Dias, Carlos Sato Baraldi, Pryor, Alan, Bouchmella, Karim, Zhao, Lingrong, Lo, Yuan Hung, Cardoso, Mateus Borba, Shapiro, David, Rodriguez, Jose, & Miao, Jianwei. Correlative cellular ptychography with functionalized nanoparticles at the Fe L-edge. United States. doi:10.1038/s41598-017-04784-5.
Gallagher-Jones, Marcus, Dias, Carlos Sato Baraldi, Pryor, Alan, Bouchmella, Karim, Zhao, Lingrong, Lo, Yuan Hung, Cardoso, Mateus Borba, Shapiro, David, Rodriguez, Jose, and Miao, Jianwei. 2017. "Correlative cellular ptychography with functionalized nanoparticles at the Fe L-edge". United States. doi:10.1038/s41598-017-04784-5. https://www.osti.gov/servlets/purl/1408491.
@article{osti_1408491,
title = {Correlative cellular ptychography with functionalized nanoparticles at the Fe L-edge},
author = {Gallagher-Jones, Marcus and Dias, Carlos Sato Baraldi and Pryor, Alan and Bouchmella, Karim and Zhao, Lingrong and Lo, Yuan Hung and Cardoso, Mateus Borba and Shapiro, David and Rodriguez, Jose and Miao, Jianwei},
abstractNote = {Precise localization of nanoparticles within a cell is crucial to the understanding of cell-particle interactions and has broad applications in nanomedicine. Here in this paper, we report a proof-of-principle experiment for imaging individual functionalized nanoparticles within a mammalian cell by correlative microscopy. Using a chemically-fixed HeLa cell labeled with fluorescent core-shell nanoparticles as a model system, we implemented a graphene-oxide layer as a substrate to significantly reduce background scattering. We identified cellular features of interest by fluorescence microscopy, followed by scanning transmission X-ray tomography to localize the particles in 3D, and ptychographic coherent diffractive imaging of the fine features in the region at high resolution. By tuning the X-ray energy to the Fe L-edge, we demonstrated sensitive detection of nanoparticles composed of a 22 nm magnetic Fe3O4 core encased by a 25-nm-thick fluorescent silica (SiO2) shell. These fluorescent core-shell nanoparticles act as landmarks and offer clarity in a cellular context. Our correlative microscopy results confirmed a subset of particles to be fully internalized, and high-contrast ptychographic images showed two oxidation states of individual nanoparticles with a resolution of ~16.5 nm. The ability to precisely localize individual fluorescent nanoparticles within mammalian cells will expand our understanding of the structure/function relationships for functionalized nanoparticles.},
doi = {10.1038/s41598-017-04784-5},
journal = {Scientific Reports},
number = 1,
volume = 7,
place = {United States},
year = {2017},
month = {7}
}