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Title: Association of Distinct Fine Specificities of Anti−Citrullinated Peptide Antibodies With Elevated Immune Responses to Prevotella intermedia in a Subgroup of Patients With Rheumatoid Arthritis and Periodontitis

Abstract

Objective In addition to the long‐established link with smoking, periodontitis ( PD ) is a risk factor for rheumatoid arthritis ( RA ). This study was undertaken to elucidate the mechanism by which PD could induce antibodies to citrullinated peptides ( ACPA s), by examining the antibody response to a novel citrullinated peptide of cytokeratin 13 ( CK ‐13) identified in gingival crevicular fluid ( GCF ), and comparing the response to 4 other citrullinated peptides in patients with RA who were well‐characterized for PD and smoking. Methods The citrullinomes of GCF and periodontal tissue from patients with PD were mapped by mass spectrometry. ACPA s of CK 13 ( cCK 13), tenascin‐C ( cTNC 5), vimentin ( cVIM ), α‐enolase ( CEP ‐1), and fibrinogen β ( cFIB β) were examined by enzyme‐linked immunosorbent assay in patients with RA (n = 287) and patients with osteoarthritis (n = 330), and cross‐reactivity was assessed by inhibition assays. Results A novel citrullinated peptide cCK 13‐1 ( 444 TSNASGR ‐Cit‐ TSDV ‐Cit‐ RP 458 ) identified in GCF exhibited elevated antibody responses in RA patients (24%). Anti– cCK 13‐1 antibody levels correlated with anti– cTNC 5 antibody levels, and absorption experiments confirmedmore » this was not due to cross‐reactivity. Only anti– cCK 13‐1 and anti‐ cTNC 5 were associated with antibodies to the periodontal pathogen Prevotella intermedia ( P = 0.05 and P = 0.001, respectively), but not with antibodies to Porphyromonas gingivalis arginine gingipains. Levels of antibodies to CEP ‐1, cFIB β, and cVIM correlated with each other, and with smoking and shared epitope risk factors in RA. Conclusion This study identifies 2 groups of ACPA fine specificities associated with different RA risk factors. One is predominantly linked to smoking and shared epitope, and the other links anti– cTNC 5 and cCK 13‐1 to infection with the periodontal pathogen P intermedia .« less

Authors:
 [1];  [1];  [2];  [3];  [1];  [4];  [5];  [3];  [3];  [3];  [1];  [6];  [1];  [1];  [1];  [7];  [8];  [1]
+ Show Author Affiliations
  1. University of Oxford Oxford UK
  2. University of Oxford, Oxford, UK, and Jagiellonian University Krakow Poland
  3. Jagiellonian University Krakow Poland
  4. University of Nebraska Medical Center Omaha
  5. University of Bern Bern Switzerland
  6. Jagiellonian University, Krakow, Poland, and University of Louisville Louisville Kentucky
  7. University of Nebraska Medical Center Lincoln
  8. University of Nebraska Medical Center and Nebraska‐Western Iowa Health Care System Omaha
Publication Date:
Sponsoring Org.:
USDOE
OSTI Identifier:
1405565
Alternate Identifier(s):
OSTI ID: 1408157
Resource Type:
Published Article
Journal Name:
Arthritis & Rheumatology
Additional Journal Information:
Journal Name: Arthritis & Rheumatology Journal Volume: 69 Journal Issue: 12; Journal ID: ISSN 2326-5191
Publisher:
Wiley Blackwell (John Wiley & Sons)
Country of Publication:
Country unknown/Code not available
Language:
English

Citation Formats

Schwenzer, Anja, Quirke, Anne‐Marie, Marzeda, Anna M., Wong, Alicia, Montgomery, Anna B., Sayles, Harlan R., Eick, Sigrun, Gawron, Katarzyna, Chomyszyn‐Gajewska, Maria, Łazarz‐Bartyzel, Katarzyna, Davis, Simon, Potempa, Jan, Kessler, Benedikt M., Fischer, Roman, Venables, Patrick J., Payne, Jeffrey B., Mikuls, Ted R., and Midwood, Kim S. Association of Distinct Fine Specificities of Anti−Citrullinated Peptide Antibodies With Elevated Immune Responses to Prevotella intermedia in a Subgroup of Patients With Rheumatoid Arthritis and Periodontitis. Country unknown/Code not available: N. p., 2017. Web. doi:10.1002/art.40227.
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Schwenzer, Anja, Quirke, Anne‐Marie, Marzeda, Anna M., Wong, Alicia, Montgomery, Anna B., Sayles, Harlan R., Eick, Sigrun, Gawron, Katarzyna, Chomyszyn‐Gajewska, Maria, Łazarz‐Bartyzel, Katarzyna, Davis, Simon, Potempa, Jan, Kessler, Benedikt M., Fischer, Roman, Venables, Patrick J., Payne, Jeffrey B., Mikuls, Ted R., & Midwood, Kim S. Association of Distinct Fine Specificities of Anti−Citrullinated Peptide Antibodies With Elevated Immune Responses to Prevotella intermedia in a Subgroup of Patients With Rheumatoid Arthritis and Periodontitis. Country unknown/Code not available. https://doi.org/10.1002/art.40227
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Schwenzer, Anja, Quirke, Anne‐Marie, Marzeda, Anna M., Wong, Alicia, Montgomery, Anna B., Sayles, Harlan R., Eick, Sigrun, Gawron, Katarzyna, Chomyszyn‐Gajewska, Maria, Łazarz‐Bartyzel, Katarzyna, Davis, Simon, Potempa, Jan, Kessler, Benedikt M., Fischer, Roman, Venables, Patrick J., Payne, Jeffrey B., Mikuls, Ted R., and Midwood, Kim S. Mon . "Association of Distinct Fine Specificities of Anti−Citrullinated Peptide Antibodies With Elevated Immune Responses to Prevotella intermedia in a Subgroup of Patients With Rheumatoid Arthritis and Periodontitis". Country unknown/Code not available. https://doi.org/10.1002/art.40227.
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@article{osti_1405565,
title = {Association of Distinct Fine Specificities of Anti−Citrullinated Peptide Antibodies With Elevated Immune Responses to Prevotella intermedia in a Subgroup of Patients With Rheumatoid Arthritis and Periodontitis},
author = {Schwenzer, Anja and Quirke, Anne‐Marie and Marzeda, Anna M. and Wong, Alicia and Montgomery, Anna B. and Sayles, Harlan R. and Eick, Sigrun and Gawron, Katarzyna and Chomyszyn‐Gajewska, Maria and Łazarz‐Bartyzel, Katarzyna and Davis, Simon and Potempa, Jan and Kessler, Benedikt M. and Fischer, Roman and Venables, Patrick J. and Payne, Jeffrey B. and Mikuls, Ted R. and Midwood, Kim S.},
abstractNote = {Objective In addition to the long‐established link with smoking, periodontitis ( PD ) is a risk factor for rheumatoid arthritis ( RA ). This study was undertaken to elucidate the mechanism by which PD could induce antibodies to citrullinated peptides ( ACPA s), by examining the antibody response to a novel citrullinated peptide of cytokeratin 13 ( CK ‐13) identified in gingival crevicular fluid ( GCF ), and comparing the response to 4 other citrullinated peptides in patients with RA who were well‐characterized for PD and smoking. Methods The citrullinomes of GCF and periodontal tissue from patients with PD were mapped by mass spectrometry. ACPA s of CK 13 ( cCK 13), tenascin‐C ( cTNC 5), vimentin ( cVIM ), α‐enolase ( CEP ‐1), and fibrinogen β ( cFIB β) were examined by enzyme‐linked immunosorbent assay in patients with RA (n = 287) and patients with osteoarthritis (n = 330), and cross‐reactivity was assessed by inhibition assays. Results A novel citrullinated peptide cCK 13‐1 ( 444 TSNASGR ‐Cit‐ TSDV ‐Cit‐ RP 458 ) identified in GCF exhibited elevated antibody responses in RA patients (24%). Anti– cCK 13‐1 antibody levels correlated with anti– cTNC 5 antibody levels, and absorption experiments confirmed this was not due to cross‐reactivity. Only anti– cCK 13‐1 and anti‐ cTNC 5 were associated with antibodies to the periodontal pathogen Prevotella intermedia ( P = 0.05 and P = 0.001, respectively), but not with antibodies to Porphyromonas gingivalis arginine gingipains. Levels of antibodies to CEP ‐1, cFIB β, and cVIM correlated with each other, and with smoking and shared epitope risk factors in RA. Conclusion This study identifies 2 groups of ACPA fine specificities associated with different RA risk factors. One is predominantly linked to smoking and shared epitope, and the other links anti– cTNC 5 and cCK 13‐1 to infection with the periodontal pathogen P intermedia .},
doi = {10.1002/art.40227},
journal = {Arthritis & Rheumatology},
number = 12,
volume = 69,
place = {Country unknown/Code not available},
year = {Mon Oct 30 00:00:00 EDT 2017},
month = {Mon Oct 30 00:00:00 EDT 2017}
}
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