Microscopic diffusion in hydrated encysted eggs of brine shrimp

Mamontov, Eugene

doi:10.1016/j.bbagen.2017.05.022

Title: Microscopic diffusion in hydrated encysted eggs of brine shrimp

Abstract

We have studied microscopic diffusion of water in fully hydrated encysted eggs of brine shrimp (Artemia). We utilized quasielastic neutron scattering. Dry eggs of brine shrimp were rehydrated using (1) water without additives, (2) eutectic mixture of water and dimethyl sulfoxide, and (3) a concentrated aqueous solution of lithium chloride. Despite the complexity of the hydrated multicellular organism, measurable microscopic diffusivity of water is rather well defined. Pure hydration water in eggs exhibits freezing temperature depression, whereas hydration water in eggs mixed with dimethyl sulfoxide or lithium chloride does not crystallize at all. The characteristic size of the voids occupied by water or aqueous solvents in hydrated brine shrimp eggs is between 2 and 10 nm. Those voids are accessible to co-solvents such as dimethyl sulfoxide and lithium chloride. There is no evidence of intracellular water in the hydrated eggs. The lack of intracellular water in the fully hydrated (but still under arrested development) state must be linked to the unique resilience against adverse environmental factors documented not only for the anhydrous, but also hydrated encysted eggs of brine shrimp.

Authors:

^[1]

Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Chemical and Engineering Materials Division

Publication Date:: Wed May 24 00:00:00 EDT 2017

Research Org.:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Org.:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

OSTI Identifier:: 1408013

Alternate Identifier(s):: OSTI ID: 1550096

Grant/Contract Number:: AC05-00OR22725

Resource Type:: Accepted Manuscript

Journal Name:: Biochimica et Biophysica Acta - General Subjects

Additional Journal Information:: Journal Volume: 1861; Journal Issue: 9; Journal ID: ISSN 0304-4165

Publisher:: Elsevier

Country of Publication:: United States

Language:: English

Subject:: 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; 54 ENVIRONMENTAL SCIENCES; Brine shrimp eggs; hydration water; aqueous solvents; microscopic dynamics

Citation Formats


                    Mamontov, Eugene. Microscopic diffusion in hydrated encysted eggs of brine shrimp.  United States: N. p., 2017. 
Web.  doi:10.1016/j.bbagen.2017.05.022.

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                    Mamontov, Eugene. Microscopic diffusion in hydrated encysted eggs of brine shrimp.  United States.  https://doi.org/10.1016/j.bbagen.2017.05.022

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                    Mamontov, Eugene. Wed .  
"Microscopic diffusion in hydrated encysted eggs of brine shrimp".  United States.  https://doi.org/10.1016/j.bbagen.2017.05.022.  https://www.osti.gov/servlets/purl/1408013.

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@article{osti_1408013,

  title        = {Microscopic diffusion in hydrated encysted eggs of brine shrimp},

  author       = {Mamontov, Eugene},

  abstractNote = {We have studied microscopic diffusion of water in fully hydrated encysted eggs of brine shrimp (Artemia). We utilized quasielastic neutron scattering. Dry eggs of brine shrimp were rehydrated using (1) water without additives, (2) eutectic mixture of water and dimethyl sulfoxide, and (3) a concentrated aqueous solution of lithium chloride. Despite the complexity of the hydrated multicellular organism, measurable microscopic diffusivity of water is rather well defined. Pure hydration water in eggs exhibits freezing temperature depression, whereas hydration water in eggs mixed with dimethyl sulfoxide or lithium chloride does not crystallize at all. The characteristic size of the voids occupied by water or aqueous solvents in hydrated brine shrimp eggs is between 2 and 10 nm. Those voids are accessible to co-solvents such as dimethyl sulfoxide and lithium chloride. There is no evidence of intracellular water in the hydrated eggs. The lack of intracellular water in the fully hydrated (but still under arrested development) state must be linked to the unique resilience against adverse environmental factors documented not only for the anhydrous, but also hydrated encysted eggs of brine shrimp.},

  doi          = {10.1016/j.bbagen.2017.05.022},

  journal      = {Biochimica et Biophysica Acta - General Subjects},

  number       = 9,

  volume       = 1861,

  place        = {United States},

  year         = {Wed May 24 00:00:00 EDT 2017},

  month        = {Wed May 24 00:00:00 EDT 2017}

}

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Works referencing / citing this record:

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Scientific Reports, Vol. 8, Issue 1
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Neutron scattering in the biological sciences: progress and prospects
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Title: Microscopic diffusion in hydrated encysted eggs of brine shrimp

Abstract

Citation Formats

Metabolic depression in animals: physiological perspectives and biochemical generalizations journal, February 1999

Water Dynamics in Neural Tissue journal, January 2013

Phase Diagram for the System Water–Dimethylsulphoxide journal, December 1968

Causes and Consequences of Excess Resistance in Cryptobiotic Metazoans journal, July 2003

Down to atomic-scale intracellular water dynamics journal, May 2008

Observation of nanophase segregation in LiCl aqueous solutions from transient grating experiments journal, July 2013

Thermal Property, Structure, and Dynamics of Supercooled Water in Porous Silica by Calorimetry, Neutron Scattering, and NMR Relaxation journal, July 1997

Structural study of water confined in porous glass by neutron scattering journal, March 1993

Water–protein dynamic coupling and new opportunities for probing it at low to physiological temperatures in aqueous solutions journal, January 2012

Organization and Flexibility of Cyanobacterial Thylakoid Membranes Examined by Neutron Scattering journal, December 2012

Melting behavior of water in cylindrical pores: carbon nanotubes and silica glasses journal, January 2008

Glass‐Forming Composition Regions and Glass Transition Temperatures for Aqueous Electrolyte Solutions journal, February 1970

Artemin, a diapause-specific chaperone, contributes to the stress tolerance of Artemia franciscana cysts and influences their release from females journal, February 2014

Prevention of Freezing Damage to Living Cells by Dimethyl Sulphoxide journal, May 1959

Fast internal dynamics in alcohol dehydrogenase journal, August 2015

Neutron diffraction study of cubic ice nucleation in a porous silica network journal, July 1983

Slow dynamics of supercooled water confined in nanoporous silica materials journal, October 2004

The two-step scenario of the protein dynamical transition journal, January 2011

Translational diffusion of hydration water correlates with functional motions in folded and intrinsically disordered proteins journal, March 2015

No fragile-to-strong crossover in LiCl-H 2 O solution journal, March 2012

Protein-Style Dynamical Transition in a Non-Biological Polymer and a Non-Aqueous Solvent journal, March 2016

Specific cellular water dynamics observed in vivo by neutron scattering and NMR journal, January 2010

Dynamical transition of myoglobin revealed by inelastic neutron scattering journal, February 1989

X-ray diffraction studies of freezing and melting of water confined in a mesoporous adsorbent (MCM-41) journal, November 1997

Dynamics of water in LiCl and CaCl2 aqueous solutions confined in silica matrices: A backscattering neutron spectroscopy study journal, September 2008

Dynamics of tRNA at Different Levels of Hydration journal, April 2009

Hydration level dependence of the microscopic dynamics of water adsorbed in ultramicroporous carbon journal, January 2017

Diffusion Dynamics of Water Molecules in a LiCl Solution: A Low-Temperature Crossover journal, October 2009

Mechanism of Cell Damage During Freezing and Thawing and its Prevention journal, March 1965

A Low-Temperature Crossover in Water Dynamics in an Aqueous LiCl Solution: Diffusion Probed by Neutron Spin−Echo and Nuclear Magnetic Resonance journal, December 2010

The structure of the shell and outer membranes in encysted Artemia salina embryos during cryptobiosis and development journal, October 1967

High protein flexibility and reduced hydration water dynamics are key pressure adaptive strategies in prokaryotes journal, September 2016

Cytoplasmic Water and Hydration Layer Dynamics in Human Red Blood Cells journal, December 2008

Single-particle dynamics in dimethyl–sulfoxide/water eutectic mixture by neutron scattering journal, November 2000

Comparative study on thermotolerance of Artemia resting eggs from Qinghai–Xizang Plateau, China journal, September 2010

A time-of-flight backscattering spectrometer at the Spallation Neutron Source, BASIS journal, August 2011

Apparent Decoupling of the Dynamics of a Protein from the Dynamics of its Aqueous Solvent journal, January 2012

Temperature dependence of the internal dynamics of a protein in an aqueous solvent: Decoupling from the solvent viscosity journal, October 2013

The origin and structure of the tertiary envelope in thick-shelled eggs of the brine shrimp, Artemia journal, September 1970

Embryos of Artemia franciscana survive four years of continuous anoxia: the case for complete metabolic rate depression journal, January 1997

Microscopic diffusion processes measured in living planarians journal, March 2018

Neutron scattering in the biological sciences: progress and prospects journal, December 2018

Metabolic depression in animals: physiological perspectives and biochemical generalizations
journal, February 1999

Water Dynamics in Neural Tissue
journal, January 2013

Phase Diagram for the System Water–Dimethylsulphoxide
journal, December 1968

Causes and Consequences of Excess Resistance in Cryptobiotic Metazoans
journal, July 2003

Down to atomic-scale intracellular water dynamics
journal, May 2008

Observation of nanophase segregation in LiCl aqueous solutions from transient grating experiments
journal, July 2013

Thermal Property, Structure, and Dynamics of Supercooled Water in Porous Silica by Calorimetry, Neutron Scattering, and NMR Relaxation
journal, July 1997

Structural study of water confined in porous glass by neutron scattering
journal, March 1993

Water–protein dynamic coupling and new opportunities for probing it at low to physiological temperatures in aqueous solutions
journal, January 2012

Organization and Flexibility of Cyanobacterial Thylakoid Membranes Examined by Neutron Scattering
journal, December 2012

Melting behavior of water in cylindrical pores: carbon nanotubes and silica glasses
journal, January 2008

Glass‐Forming Composition Regions and Glass Transition Temperatures for Aqueous Electrolyte Solutions
journal, February 1970

Artemin, a diapause-specific chaperone, contributes to the stress tolerance of Artemia franciscana cysts and influences their release from females
journal, February 2014

Prevention of Freezing Damage to Living Cells by Dimethyl Sulphoxide
journal, May 1959

Fast internal dynamics in alcohol dehydrogenase
journal, August 2015

Neutron diffraction study of cubic ice nucleation in a porous silica network
journal, July 1983

Slow dynamics of supercooled water confined in nanoporous silica materials
journal, October 2004

The two-step scenario of the protein dynamical transition
journal, January 2011

Translational diffusion of hydration water correlates with functional motions in folded and intrinsically disordered proteins
journal, March 2015

No fragile-to-strong crossover in LiCl-H ₂ O solution
journal, March 2012

Protein-Style Dynamical Transition in a Non-Biological Polymer and a Non-Aqueous Solvent
journal, March 2016

Specific cellular water dynamics observed in vivo by neutron scattering and NMR
journal, January 2010

Dynamical transition of myoglobin revealed by inelastic neutron scattering
journal, February 1989

X-ray diffraction studies of freezing and melting of water confined in a mesoporous adsorbent (MCM-41)
journal, November 1997

Dynamics of water in LiCl and CaCl2 aqueous solutions confined in silica matrices: A backscattering neutron spectroscopy study
journal, September 2008

Dynamics of tRNA at Different Levels of Hydration
journal, April 2009

Hydration level dependence of the microscopic dynamics of water adsorbed in ultramicroporous carbon
journal, January 2017

Diffusion Dynamics of Water Molecules in a LiCl Solution: A Low-Temperature Crossover
journal, October 2009

Mechanism of Cell Damage During Freezing and Thawing and its Prevention
journal, March 1965

A Low-Temperature Crossover in Water Dynamics in an Aqueous LiCl Solution: Diffusion Probed by Neutron Spin−Echo and Nuclear Magnetic Resonance
journal, December 2010

The structure of the shell and outer membranes in encysted Artemia salina embryos during cryptobiosis and development
journal, October 1967

High protein flexibility and reduced hydration water dynamics are key pressure adaptive strategies in prokaryotes
journal, September 2016

Cytoplasmic Water and Hydration Layer Dynamics in Human Red Blood Cells
journal, December 2008

Single-particle dynamics in dimethyl–sulfoxide/water eutectic mixture by neutron scattering
journal, November 2000

Comparative study on thermotolerance of Artemia resting eggs from Qinghai–Xizang Plateau, China
journal, September 2010

A time-of-flight backscattering spectrometer at the Spallation Neutron Source, BASIS
journal, August 2011

Apparent Decoupling of the Dynamics of a Protein from the Dynamics of its Aqueous Solvent
journal, January 2012

Temperature dependence of the internal dynamics of a protein in an aqueous solvent: Decoupling from the solvent viscosity
journal, October 2013

The origin and structure of the tertiary envelope in thick-shelled eggs of the brine shrimp, Artemia
journal, September 1970

Embryos of Artemia franciscana survive four years of continuous anoxia: the case for complete metabolic rate depression
journal, January 1997

Microscopic diffusion processes measured in living planarians
journal, March 2018

Neutron scattering in the biological sciences: progress and prospects
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