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Title: Genome Characterization of Oleaginous Aspergillus oryzae BCC7051: A Potential Fungal-Based Platform for Lipid Production

Abstract

The selected robust fungus, Aspergillus oryzae strain BCC7051 is of interest for biotechnological production of lipid-derived products due to its capability to accumulate high amount of intracellular lipids using various sugars and agro-industrial substrates. Here in this paper, we report the genome sequence of the oleaginous A. oryzae BCC7051. The obtained reads were de novo assembled into 25 scaffolds spanning of 38,550,958 bps with predicted 11,456 protein-coding genes. By synteny mapping, a large rearrangement was found in two scaffolds of A. oryzae BCC7051 as compared to the reference RIB40 strain. The genetic relationship between BCC7051 and other strains of A. oryzae in terms of aflatoxin production was investigated, indicating that the A. oryzae BCC7051 was categorized into group 2 nonaflatoxin-producing strain. Moreover, a comparative analysis of the structural genes focusing on the involvement in lipid metabolism among oleaginous yeast and fungi revealed the presence of multiple isoforms of metabolic enzymes responsible for fatty acid synthesis in BCC7051. The alternative routes of acetyl-CoA generation as oleaginous features and malate/citrate/pyruvate shuttle were also identified in this A. oryzae strain. The genome sequence generated in this work is a dedicated resource for expanding genome-wide study of microbial lipids at systems level, and developingmore » the fungal-based platform for production of diversified lipids with commercial relevance.« less

Authors:
 [1];  [2];  [1];  [3]; ORCiD logo [4];  [5];  [5]
  1. King Mongkut's Univ., of Technology Thonburi, Bangkok (Thailand). Biochemical Engineering and Pilot Plant Research and Development Lab., National Center for Genetic Engineering and Biotechnology (BIOTEC)
  2. Univ. of Arkansas for Medical Sciences, FLittle Rock, AR (United States). College of Medicine, Dept. of Biomedical Informatics; Chalmers Univ. of Technology Gothenburg (Sweden). Dept. of Biology and Biological Engineering; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Biosciences Division
  3. King Mongkut's Univ., of Technology Thonburi, Bangkok (Thailand). Pilot Plant Development and Training Inst.
  4. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Biosciences Division
  5. National Science and Technology Development Agency (NSTDA), Pathum Thani (Thailand). National Center for Genetic Engineering and Biotechnology (BIOTEC),Bioprocess Technology Lab., Food Biotechnology Research Unit
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE; Swedish Research Council (SRC)
OSTI Identifier:
1407741
Grant/Contract Number:  
AC05-00OR22725
Resource Type:
Accepted Manuscript
Journal Name:
Current Microbiology (Print)
Additional Journal Information:
Journal Name: Current Microbiology (Print); Journal Volume: 75; Journal Issue: 1; Journal ID: ISSN 0343-8651
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES

Citation Formats

Thammarongtham, Chinae, Nookaew, Intawat, Vorapreeda, Tayvich, Srisuk, Tanawut, Land, Miriam L., Jeennor, Sukanya, and Laoteng, Kobkul. Genome Characterization of Oleaginous Aspergillus oryzae BCC7051: A Potential Fungal-Based Platform for Lipid Production. United States: N. p., 2017. Web. doi:10.1007/s00284-017-1350-7.
Thammarongtham, Chinae, Nookaew, Intawat, Vorapreeda, Tayvich, Srisuk, Tanawut, Land, Miriam L., Jeennor, Sukanya, & Laoteng, Kobkul. Genome Characterization of Oleaginous Aspergillus oryzae BCC7051: A Potential Fungal-Based Platform for Lipid Production. United States. doi:10.1007/s00284-017-1350-7.
Thammarongtham, Chinae, Nookaew, Intawat, Vorapreeda, Tayvich, Srisuk, Tanawut, Land, Miriam L., Jeennor, Sukanya, and Laoteng, Kobkul. Fri . "Genome Characterization of Oleaginous Aspergillus oryzae BCC7051: A Potential Fungal-Based Platform for Lipid Production". United States. doi:10.1007/s00284-017-1350-7. https://www.osti.gov/servlets/purl/1407741.
@article{osti_1407741,
title = {Genome Characterization of Oleaginous Aspergillus oryzae BCC7051: A Potential Fungal-Based Platform for Lipid Production},
author = {Thammarongtham, Chinae and Nookaew, Intawat and Vorapreeda, Tayvich and Srisuk, Tanawut and Land, Miriam L. and Jeennor, Sukanya and Laoteng, Kobkul},
abstractNote = {The selected robust fungus, Aspergillus oryzae strain BCC7051 is of interest for biotechnological production of lipid-derived products due to its capability to accumulate high amount of intracellular lipids using various sugars and agro-industrial substrates. Here in this paper, we report the genome sequence of the oleaginous A. oryzae BCC7051. The obtained reads were de novo assembled into 25 scaffolds spanning of 38,550,958 bps with predicted 11,456 protein-coding genes. By synteny mapping, a large rearrangement was found in two scaffolds of A. oryzae BCC7051 as compared to the reference RIB40 strain. The genetic relationship between BCC7051 and other strains of A. oryzae in terms of aflatoxin production was investigated, indicating that the A. oryzae BCC7051 was categorized into group 2 nonaflatoxin-producing strain. Moreover, a comparative analysis of the structural genes focusing on the involvement in lipid metabolism among oleaginous yeast and fungi revealed the presence of multiple isoforms of metabolic enzymes responsible for fatty acid synthesis in BCC7051. The alternative routes of acetyl-CoA generation as oleaginous features and malate/citrate/pyruvate shuttle were also identified in this A. oryzae strain. The genome sequence generated in this work is a dedicated resource for expanding genome-wide study of microbial lipids at systems level, and developing the fungal-based platform for production of diversified lipids with commercial relevance.},
doi = {10.1007/s00284-017-1350-7},
journal = {Current Microbiology (Print)},
number = 1,
volume = 75,
place = {United States},
year = {2017},
month = {9}
}

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