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Title: Regulation of photosystem I light harvesting by zeaxanthin

In oxygenic photosynthetic eukaryotes, the hydroxylated carotenoid zeaxanthin is produced from preexisting violaxanthin upon exposure to excess light conditions. Zeaxanthin binding to components of the photosystem II (PSII) antenna system has been investigated thoroughly and shown to help in the dissipation of excess chlorophyll-excited states and scavenging of oxygen radicals. However, the functional consequences of the accumulation of the light-harvesting complex I (LHCI) proteins in the photosystem I (PSI) antenna have remained unclarified so far. In this paper we investigated the effect of zeaxanthin binding on photoprotection of PSI–LHCI by comparing preparations isolated from wild-type Arabidopsis thaliana (i.e., with violaxanthin) and those isolated from the A. thaliana nonphotochemical quenching 2 mutant, in which violaxanthin is replaced by zeaxanthin. Time-resolved fluorescence measurements showed that zeaxanthin binding leads to a previously unrecognized quenching effect on PSI–LHCI fluorescence. The efficiency of energy transfer from the LHCI moiety of the complex to the PSI reaction center was down-regulated, and an enhanced PSI resistance to photoinhibition was observed both in vitro and in vivo. Thus, zeaxanthin was shown to be effective in inducing dissipative states in PSI, similar to its well-known effect on PSII. Finally, we propose that, upon acclimation to high light, PSI–LHCI changesmore » its light-harvesting efficiency by a zeaxanthin-dependent quenching of the absorbed excitation energy, whereas in PSII the stoichiometry of LHC antenna proteins per reaction center is reduced directly.« less
Authors:
 [1] ;  [2] ;  [2] ;  [3] ;  [4] ;  [5] ;  [2] ;  [6] ;  [3] ;  [1]
  1. Univ. of Verona (Italy). Dept. of Biotechnology
  2. Polytechnic Univ. of Milan (Italy). Center for Nanoscience and Technology. Italian Inst. of Technology. Inst. for Photonics and Nanotechnology. National Research Council. Dept. of Physics
  3. Polytechnic Univ. of Milan (Italy). Inst. for Photonics and Nanotechnology. National Research Council. Dept. of Physics
  4. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Physical Biosciences Division; Sungkyunkwan Univ., Suwon (Republic of Korea). Dept. of Energy Science; Univ. of California, Berkeley, CA (United States). Dept. of Chemistry
  5. Polytechnic Univ. of Milan (Italy). Center for Nanoscience and Technology. Italian Inst. of Technology
  6. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Physical Biosciences Division; Univ. of California, Berkeley, CA (United States). Dept. of Chemistry
Publication Date:
Grant/Contract Number:
AC02-05CH11231; 316427; RBFR08XH0H; PJ00910603201
Type:
Accepted Manuscript
Journal Name:
Proceedings of the National Academy of Sciences of the United States of America
Additional Journal Information:
Journal Volume: 111; Journal Issue: 23; Journal ID: ISSN 0027-8424
Publisher:
National Academy of Sciences, Washington, DC (United States)
Research Org:
Univ. of Verona (Italy); Polytechnic Univ. of Milan (Italy); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22); European Union (EU); Ministry of Agriculture, Food and Forestry (Italy); Ministry for Education, Higher Education and Future Research (Italy); Korean Rural Development Agency (Republic of Korea)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; photosynthesis; xanthophylls; violaxanthin de-epoxidase; photobleaching
OSTI Identifier:
1407239

Ballottari, Matteo, Alcocer, Marcelo J. P., D'Andrea, Cosimo, Viola, Daniele, Ahn, Tae Kyu, Petrozza, Annamaria, Polli, Dario, Fleming, Graham R., Cerullo, Giulio, and Bassi, Roberto. Regulation of photosystem I light harvesting by zeaxanthin. United States: N. p., Web. doi:10.1073/pnas.1404377111.
Ballottari, Matteo, Alcocer, Marcelo J. P., D'Andrea, Cosimo, Viola, Daniele, Ahn, Tae Kyu, Petrozza, Annamaria, Polli, Dario, Fleming, Graham R., Cerullo, Giulio, & Bassi, Roberto. Regulation of photosystem I light harvesting by zeaxanthin. United States. doi:10.1073/pnas.1404377111.
Ballottari, Matteo, Alcocer, Marcelo J. P., D'Andrea, Cosimo, Viola, Daniele, Ahn, Tae Kyu, Petrozza, Annamaria, Polli, Dario, Fleming, Graham R., Cerullo, Giulio, and Bassi, Roberto. 2014. "Regulation of photosystem I light harvesting by zeaxanthin". United States. doi:10.1073/pnas.1404377111. https://www.osti.gov/servlets/purl/1407239.
@article{osti_1407239,
title = {Regulation of photosystem I light harvesting by zeaxanthin},
author = {Ballottari, Matteo and Alcocer, Marcelo J. P. and D'Andrea, Cosimo and Viola, Daniele and Ahn, Tae Kyu and Petrozza, Annamaria and Polli, Dario and Fleming, Graham R. and Cerullo, Giulio and Bassi, Roberto},
abstractNote = {In oxygenic photosynthetic eukaryotes, the hydroxylated carotenoid zeaxanthin is produced from preexisting violaxanthin upon exposure to excess light conditions. Zeaxanthin binding to components of the photosystem II (PSII) antenna system has been investigated thoroughly and shown to help in the dissipation of excess chlorophyll-excited states and scavenging of oxygen radicals. However, the functional consequences of the accumulation of the light-harvesting complex I (LHCI) proteins in the photosystem I (PSI) antenna have remained unclarified so far. In this paper we investigated the effect of zeaxanthin binding on photoprotection of PSI–LHCI by comparing preparations isolated from wild-type Arabidopsis thaliana (i.e., with violaxanthin) and those isolated from the A. thaliana nonphotochemical quenching 2 mutant, in which violaxanthin is replaced by zeaxanthin. Time-resolved fluorescence measurements showed that zeaxanthin binding leads to a previously unrecognized quenching effect on PSI–LHCI fluorescence. The efficiency of energy transfer from the LHCI moiety of the complex to the PSI reaction center was down-regulated, and an enhanced PSI resistance to photoinhibition was observed both in vitro and in vivo. Thus, zeaxanthin was shown to be effective in inducing dissipative states in PSI, similar to its well-known effect on PSII. Finally, we propose that, upon acclimation to high light, PSI–LHCI changes its light-harvesting efficiency by a zeaxanthin-dependent quenching of the absorbed excitation energy, whereas in PSII the stoichiometry of LHC antenna proteins per reaction center is reduced directly.},
doi = {10.1073/pnas.1404377111},
journal = {Proceedings of the National Academy of Sciences of the United States of America},
number = 23,
volume = 111,
place = {United States},
year = {2014},
month = {5}
}