Automated particle correspondence and accurate tilt-axis detection in tilted-image pairs

Shatsky, Maxim; Arbelaez, Pablo; Han, Bong-Gyoon; Typke, Dieter; Brenner, Steven E.; Malik, Jitendra; Glaeser, Robert M.

doi:10.1016/j.jsb.2014.03.017

Title: Automated particle correspondence and accurate tilt-axis detection in tilted-image pairs

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Abstract

Tilted electron microscope images are routinely collected for an ab initio structure reconstruction as a part of the Random Conical Tilt (RCT) or Orthogonal Tilt Reconstruction (OTR) methods, as well as for various applications using the "free-hand" procedure. These procedures all require identification of particle pairs in two corresponding images as well as accurate estimation of the tilt-axis used to rotate the electron microscope (EM) grid. Here we present a computational approach, PCT (particle correspondence from tilted pairs), based on tilt-invariant context and projection matching that addresses both problems. The method benefits from treating the two problems as a single optimization task. It automatically finds corresponding particle pairs and accurately computes tilt-axis direction even in the cases when EM grid is not perfectly planar.

Authors:: Shatsky, Maxim; Arbelaez, Pablo; Han, Bong-Gyoon; Typke, Dieter; Brenner, Steven E.; Malik, Jitendra; Glaeser, Robert M.

Publication Date:: Tue Jul 01 00:00:00 EDT 2014

Research Org.:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Org.:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

OSTI Identifier:: 1198096

Alternate Identifier(s):: OSTI ID: 1407218

Grant/Contract Number:: AC02-05CH11231

Resource Type:: Published Article

Journal Name:: Journal of Structural Biology

Additional Journal Information:: Journal Name: Journal of Structural Biology Journal Volume: 187 Journal Issue: 1; Journal ID: ISSN 1047-8477

Publisher:: Elsevier

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES

Citation Formats


                    Shatsky, Maxim, Arbelaez, Pablo, Han, Bong-Gyoon, Typke, Dieter, Brenner, Steven E., Malik, Jitendra, and Glaeser, Robert M. Automated particle correspondence and accurate tilt-axis detection in tilted-image pairs.  United States: N. p., 2014. 
Web.  doi:10.1016/j.jsb.2014.03.017.

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                    Shatsky, Maxim, Arbelaez, Pablo, Han, Bong-Gyoon, Typke, Dieter, Brenner, Steven E., Malik, Jitendra, & Glaeser, Robert M. Automated particle correspondence and accurate tilt-axis detection in tilted-image pairs.  United States.  https://doi.org/10.1016/j.jsb.2014.03.017

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                    Shatsky, Maxim, Arbelaez, Pablo, Han, Bong-Gyoon, Typke, Dieter, Brenner, Steven E., Malik, Jitendra, and Glaeser, Robert M. Tue .  
"Automated particle correspondence and accurate tilt-axis detection in tilted-image pairs".  United States.  https://doi.org/10.1016/j.jsb.2014.03.017.

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@article{osti_1198096,

  title        = {Automated particle correspondence and accurate tilt-axis detection in tilted-image pairs},

  author       = {Shatsky, Maxim and Arbelaez, Pablo and Han, Bong-Gyoon and Typke, Dieter and Brenner, Steven E. and Malik, Jitendra and Glaeser, Robert M.},

  abstractNote = {Tilted electron microscope images are routinely collected for an ab initio structure reconstruction as a part of the Random Conical Tilt (RCT) or Orthogonal Tilt Reconstruction (OTR) methods, as well as for various applications using the "free-hand" procedure. These procedures all require identification of particle pairs in two corresponding images as well as accurate estimation of the tilt-axis used to rotate the electron microscope (EM) grid. Here we present a computational approach, PCT (particle correspondence from tilted pairs), based on tilt-invariant context and projection matching that addresses both problems. The method benefits from treating the two problems as a single optimization task. It automatically finds corresponding particle pairs and accurately computes tilt-axis direction even in the cases when EM grid is not perfectly planar.},

  doi          = {10.1016/j.jsb.2014.03.017},

  journal      = {Journal of Structural Biology},

  number       = 1,

  volume       = 187,

  place        = {United States},

  year         = {Tue Jul 01 00:00:00 EDT 2014},

  month        = {Tue Jul 01 00:00:00 EDT 2014}

}

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