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Title: Emissions of putative isoprene oxidation products from mango branches under abiotic stress

Abstract

Although several per cent of net carbon assimilation can be re-released as isoprene emissions to the atmosphere by many tropical plants, much uncertainty remains regarding its biological significance. In a previous study, we detected emissions of isoprene and its oxidation products methyl vinyl ketone (MVK) and methacrolein (MACR) from tropical plants under high temperature/light stress, suggesting that isoprene is oxidized not only in the atmosphere but also within plants. However, a comprehensive analysis of the suite of isoprene oxidation products in plants has not been performed and production relationships with environmental stress have not been described. In this study, putative isoprene oxidation products from mango (Mangifera indica) branches under abiotic stress were first identified. High temperature/light and freeze–thaw treatments verified direct emissions of the isoprene oxidation products MVK and MACR together with the first observations of 3-methyl furan (3-MF) and 2-methyl-3-buten-2-ol (MBO) as putative novel isoprene oxidation products. Mechanical wounding also stimulated emissions of MVK and MACR. Photosynthesis under 13CO2 resulted in rapid (<30min) labelling of up to five carbon atoms of isoprene, with a similar labelling pattern observed in the putative oxidation products. These observations highlight the need to investigate further the mechanisms of isoprene oxidation within plants undermore » stress and its biological and atmospheric significance.« less

Authors:
 [1];  [2];  [3];  [4];  [5];  [6];  [7];  [7];  [8];  [1]
  1. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Climate Science Dept. Earth Science Division
  2. USDA Agriculture Research Service, Tucson, AZ (United States)
  3. Univ. of Arizona, Tucson, AZ (United States). Dept. of Chemistry & Biochemistry. Dept. of Soil, Water and Environmental Science
  4. National Inst. of Amazonian Research (INPA), Manaus (Brazil). Large-Scale Biosphere-Atmosphere Experiment (LBA)
  5. National Inst. of Amazonian Research (INPA), Manaus (Brazil). Large-Scale Biosphere-Atmosphere Experiment (LBA); Max Planck Inst. for Chemistry, Mainz (Germany). Biogeochemistry Dept.
  6. Max Planck Inst. for Chemistry, Mainz (Germany). Biogeochemistry Dept.
  7. National Center for Atmospheric Research, Boulder, CO (United States). Atmospheric Chemistry Division
  8. Univ. of Queensland, Brisbane, QLD (Australia). Australian Inst. for Bioengineering and Nanotechnology
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23); National Science Foundation (NSF)
OSTI Identifier:
1407194
Grant/Contract Number:  
AC02-05CH11231; CHE 0216226
Resource Type:
Accepted Manuscript
Journal Name:
Journal of Experimental Botany
Additional Journal Information:
Journal Volume: 64; Journal Issue: 12; Journal ID: ISSN 0022-0957
Publisher:
Oxford University Press
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 2-Methyl-3-buten-2-ol; 3-methyl furan; methacrolein; methyl vinyl ketone; reactive oxygen species; volatile organic compounds

Citation Formats

Jardine, Kolby J., Meyers, Kimberly, Abrell, Leif, Alves, Eliane G., Yanez Serrano, Ana Maria, Kesselmeier, Jürgen, Karl, Thomas, Guenther, Alex, Vickers, Claudia, and Chambers, Jeffrey Q. Emissions of putative isoprene oxidation products from mango branches under abiotic stress. United States: N. p., 2013. Web. doi:10.1093/jxb/ert202.
Jardine, Kolby J., Meyers, Kimberly, Abrell, Leif, Alves, Eliane G., Yanez Serrano, Ana Maria, Kesselmeier, Jürgen, Karl, Thomas, Guenther, Alex, Vickers, Claudia, & Chambers, Jeffrey Q. Emissions of putative isoprene oxidation products from mango branches under abiotic stress. United States. doi:10.1093/jxb/ert202.
Jardine, Kolby J., Meyers, Kimberly, Abrell, Leif, Alves, Eliane G., Yanez Serrano, Ana Maria, Kesselmeier, Jürgen, Karl, Thomas, Guenther, Alex, Vickers, Claudia, and Chambers, Jeffrey Q. Tue . "Emissions of putative isoprene oxidation products from mango branches under abiotic stress". United States. doi:10.1093/jxb/ert202. https://www.osti.gov/servlets/purl/1407194.
@article{osti_1407194,
title = {Emissions of putative isoprene oxidation products from mango branches under abiotic stress},
author = {Jardine, Kolby J. and Meyers, Kimberly and Abrell, Leif and Alves, Eliane G. and Yanez Serrano, Ana Maria and Kesselmeier, Jürgen and Karl, Thomas and Guenther, Alex and Vickers, Claudia and Chambers, Jeffrey Q.},
abstractNote = {Although several per cent of net carbon assimilation can be re-released as isoprene emissions to the atmosphere by many tropical plants, much uncertainty remains regarding its biological significance. In a previous study, we detected emissions of isoprene and its oxidation products methyl vinyl ketone (MVK) and methacrolein (MACR) from tropical plants under high temperature/light stress, suggesting that isoprene is oxidized not only in the atmosphere but also within plants. However, a comprehensive analysis of the suite of isoprene oxidation products in plants has not been performed and production relationships with environmental stress have not been described. In this study, putative isoprene oxidation products from mango (Mangifera indica) branches under abiotic stress were first identified. High temperature/light and freeze–thaw treatments verified direct emissions of the isoprene oxidation products MVK and MACR together with the first observations of 3-methyl furan (3-MF) and 2-methyl-3-buten-2-ol (MBO) as putative novel isoprene oxidation products. Mechanical wounding also stimulated emissions of MVK and MACR. Photosynthesis under 13CO2 resulted in rapid (<30min) labelling of up to five carbon atoms of isoprene, with a similar labelling pattern observed in the putative oxidation products. These observations highlight the need to investigate further the mechanisms of isoprene oxidation within plants under stress and its biological and atmospheric significance.},
doi = {10.1093/jxb/ert202},
journal = {Journal of Experimental Botany},
number = 12,
volume = 64,
place = {United States},
year = {2013},
month = {7}
}

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    Works referencing / citing this record:

    A mechanism for biogenic production and emission of MEK from MVK decoupled from isoprene biosynthesis
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    • Cappellin, Luca; Loreto, Francesco; Biasioli, Franco
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