Comprehensive functional characterization of the Glycoside Hydrolase Family 3 enzymes from Cellvibrio japonicus reveals unique metabolic roles in biomass saccharification
Abstract
Here, lignocellulose degradation is central to the carbon cycle and renewable biotechnologies. The xyloglucan (XyG), β(1!3)/β(1!4) mixed-linkage glucan (MLG), and β(1!3) glucan components of lignocellulose represent significant carbohydrate energy sources for saprophytic microorganisms. The bacterium Cellvibrio japonicus has a robust capacity for plant polysaccharide degradation, due to a genome encoding a large contingent of Carbohydrate-Active Enzymes (CAZymes), many of whose specific functions remain unknown. Using a comprehensive genetic and biochemical approach we have delineated the physiological roles of the four C. japonicus Glycoside Hydrolase Family 3 (GH3) members on diverse β-glucans. Despite high protein sequence similarity and partially overlapping activity profiles on disaccharides, these β-glucosidases are not functionally equivalent. Bgl3A has a major role in MLG and sophorose utilization, and supports β(1!3) glucan utilization, while Bgl3B underpins cellulose utilization and supports MLG utilization. Bgl3C drives β(1!3) glucan utilization. Finally, Bgl3D is the crucial β-glucosidase for XyG utilization. This study not only sheds the light on the metabolic machinery of C. japonicus, but also expands the repertoire of characterized CAZymes for future deployment in biotechnological applications. In particular, the precise functional analysis provided here serves as a reference for informed bioinformatics on the genomes of other Cellvibrio and related species.
- Authors:
-
[1]
- Univ. of Maryland Baltimore County (UMBC), Baltimore, MD (United States)
- Univ. of British Columbia, Vancouver (Canada)
- Newcastle Univ., Newcastle Upon Tyne (United Kingdom)
- Publication Date:
- Research Org.:
- Univ. of Maryland Baltimore County (UMBC), Baltimore, MD (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- OSTI Identifier:
- 1406244
- Grant/Contract Number:
- SC0014183
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Environmental Microbiology
- Additional Journal Information:
- Journal Volume: 19; Journal ID: ISSN 1462-2912
- Publisher:
- Wiley
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 09 BIOMASS FUELS; β-glucosidase; Cellvibrio japonicus; lignocellulose; mixed-linkage glucan; xyloglucan
Citation Formats
Nelson, Cassandra E., Attia, Mohamed A., Rogowski, Artur, Morland, Carl, Brumer, Harry, and Gardner, Jeffrey G. Comprehensive functional characterization of the Glycoside Hydrolase Family 3 enzymes from Cellvibrio japonicus reveals unique metabolic roles in biomass saccharification. United States: N. p., 2017.
Web. doi:10.1111/1462-2920.13959.
Nelson, Cassandra E., Attia, Mohamed A., Rogowski, Artur, Morland, Carl, Brumer, Harry, & Gardner, Jeffrey G. Comprehensive functional characterization of the Glycoside Hydrolase Family 3 enzymes from Cellvibrio japonicus reveals unique metabolic roles in biomass saccharification. United States. https://doi.org/10.1111/1462-2920.13959
Nelson, Cassandra E., Attia, Mohamed A., Rogowski, Artur, Morland, Carl, Brumer, Harry, and Gardner, Jeffrey G. Fri .
"Comprehensive functional characterization of the Glycoside Hydrolase Family 3 enzymes from Cellvibrio japonicus reveals unique metabolic roles in biomass saccharification". United States. https://doi.org/10.1111/1462-2920.13959. https://www.osti.gov/servlets/purl/1406244.
@article{osti_1406244,
title = {Comprehensive functional characterization of the Glycoside Hydrolase Family 3 enzymes from Cellvibrio japonicus reveals unique metabolic roles in biomass saccharification},
author = {Nelson, Cassandra E. and Attia, Mohamed A. and Rogowski, Artur and Morland, Carl and Brumer, Harry and Gardner, Jeffrey G.},
abstractNote = {Here, lignocellulose degradation is central to the carbon cycle and renewable biotechnologies. The xyloglucan (XyG), β(1!3)/β(1!4) mixed-linkage glucan (MLG), and β(1!3) glucan components of lignocellulose represent significant carbohydrate energy sources for saprophytic microorganisms. The bacterium Cellvibrio japonicus has a robust capacity for plant polysaccharide degradation, due to a genome encoding a large contingent of Carbohydrate-Active Enzymes (CAZymes), many of whose specific functions remain unknown. Using a comprehensive genetic and biochemical approach we have delineated the physiological roles of the four C. japonicus Glycoside Hydrolase Family 3 (GH3) members on diverse β-glucans. Despite high protein sequence similarity and partially overlapping activity profiles on disaccharides, these β-glucosidases are not functionally equivalent. Bgl3A has a major role in MLG and sophorose utilization, and supports β(1!3) glucan utilization, while Bgl3B underpins cellulose utilization and supports MLG utilization. Bgl3C drives β(1!3) glucan utilization. Finally, Bgl3D is the crucial β-glucosidase for XyG utilization. This study not only sheds the light on the metabolic machinery of C. japonicus, but also expands the repertoire of characterized CAZymes for future deployment in biotechnological applications. In particular, the precise functional analysis provided here serves as a reference for informed bioinformatics on the genomes of other Cellvibrio and related species.},
doi = {10.1111/1462-2920.13959},
journal = {Environmental Microbiology},
number = ,
volume = 19,
place = {United States},
year = {Fri Oct 20 00:00:00 EDT 2017},
month = {Fri Oct 20 00:00:00 EDT 2017}
}
Free Publicly Available Full Text
Publisher's Version of Record
Other availability
Search WorldCat to find libraries that may hold this journal
Cited by: 18 works
Citation information provided by
Web of Science
Web of Science
Figures / Tables:
Table 1: Kinetic parameters of C. japonicus GH3 enzymes against different glucodisaccharides, cellotetraose and xyloglucan based oligosaccharides.a
All figures and tables
(7 total)
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.
Works referenced in this record:
The Structure and Function of an Arabinan-specific α-1,2-Arabinofuranosidase Identified from Screening the Activities of Bacterial GH43 Glycoside Hydrolases
journal, February 2011
- Cartmell, Alan; McKee, Lauren S.; Peña, Maria J.
- Journal of Biological Chemistry, Vol. 286, Issue 17
In-Frame Deletions Allow Functional Characterization of Complex Cellulose Degradation Phenotypes in Cellvibrio japonicus
journal, June 2015
- Nelson, Cassandra E.; Gardner, Jeffrey G.
- Applied and Environmental Microbiology, Vol. 81, Issue 17
Adiposity, gut microbiota and faecal short chain fatty acids are linked in adult humans
journal, June 2014
- Fernandes, J.; Su, W.; Rahat-Rozenbloom, S.
- Nutrition & Diabetes, Vol. 4, Issue 6
Enhancement of fermentable sugar yields by α-xylosidase supplementation of commercial cellulases
journal, January 2013
- Jabbour, Dina; Borrusch, Melissa S.; Banerjee, Goutami
- Biotechnology for Biofuels, Vol. 6, Issue 1
Bacterial Cyclic β-(1,2)-Glucan Acts in Systemic Suppression of Plant Immune Responses
journal, June 2007
- Rigano, Luciano Ariel; Payette, Caroline; Brouillard, Geneviève
- The Plant Cell, Vol. 19, Issue 6
Systems analysis in Cellvibrio japonicus resolves predicted redundancy of β-glucosidases and determines essential physiological functions : Functional analysis of
journal, February 2017
- Nelson, Cassandra E.; Rogowski, Artur; Morland, Carl
- Molecular Microbiology, Vol. 104, Issue 2
Hemicelluloses
journal, June 2010
- Scheller, Henrik Vibe; Ulvskov, Peter
- Annual Review of Plant Biology, Vol. 61, Issue 1
Evolution and Diversity of Plant Cell Walls: From Algae to Flowering Plants
journal, June 2011
- Popper, Zoë A.; Michel, Gurvan; Hervé, Cécile
- Annual Review of Plant Biology, Vol. 62, Issue 1
Functional and structural characterization of a potent GH 74 endo ‐xyloglucanase from the soil saprophyte Cellvibrio japonicus unravels the first step of xyloglucan degradation
journal, January 2016
- Attia, Mohamed; Stepper, Judith; Davies, Gideon J.
- The FEBS Journal, Vol. 283, Issue 9
Properties, Chemistry, and Applications of the Bioactive Polysaccharide Curdlan
journal, March 2014
- Zhang, Ruoran; Edgar, Kevin J.
- Biomacromolecules, Vol. 15, Issue 4
Understanding How the Complex Molecular Architecture of Mannan-degrading Hydrolases Contributes to Plant Cell Wall Degradation
journal, December 2013
- Zhang, Xiaoyang; Rogowski, Artur; Zhao, Lei
- Journal of Biological Chemistry, Vol. 289, Issue 4
A complex gene locus enables xyloglucan utilization in the model saprophyte C ellvibrio japonicus
journal, September 2014
- Larsbrink, Johan; Thompson, Andrew J.; Lundqvist, Magnus
- Molecular Microbiology, Vol. 94, Issue 2
Kinetic Analyses of Retaining endo -(Xylo)glucanases from Plant and Microbial Sources Using New Chromogenic Xylogluco-Oligosaccharide Aryl Glycosides †
journal, July 2008
- Ibatullin, Farid M.; Baumann, Martin J.; Greffe, Lionel
- Biochemistry, Vol. 47, Issue 29
1,003 reference genomes of bacterial and archaeal isolates expand coverage of the tree of life
journal, June 2017
- Mukherjee, Supratim; Seshadri, Rekha; Varghese, Neha J.
- Nature Biotechnology, Vol. 35, Issue 7
Effect of Nature and Supply of Carbon Sources on Cellulase Formation in Pseudomonas fluorescens var. cellulosa
journal, January 1970
- Yamane, Kunio; Suzuki, Hiroshi; Hirotani, Masao
- The Journal of Biochemistry, Vol. 67, Issue 1
Exploring the evolution of (1,3;1,4)-β-d-glucans in plant cell walls: comparative genomics can help!
journal, April 2009
- Fincher, Geoffrey B.
- Current Opinion in Plant Biology, Vol. 12, Issue 2
Callose biosynthesis in arabidopsis with a focus on pathogen response: what we have learned within the last decade
journal, July 2014
- Ellinger, Dorothea; Voigt, Christian A.
- Annals of Botany, Vol. 114, Issue 6
Recent structural insights into the enzymology of the ubiquitous plant cell wall glycan xyloglucan
journal, October 2016
- Attia, Mohamed A.; Brumer, Harry
- Current Opinion in Structural Biology, Vol. 40
Brucella abortus Cyclic -1,2-Glucan Mutants Have Reduced Virulence in Mice and Are Defective in Intracellular Replication in HeLa Cells
journal, July 2001
- Briones, G.; Inon de Iannino, N.; Roset, M.
- Infection and Immunity, Vol. 69, Issue 7
Biomass Recalcitrance: Engineering Plants and Enzymes for Biofuels Production
journal, February 2007
- Himmel, M. E.; Ding, S.-Y.; Johnson, D. K.
- Science, Vol. 315, Issue 5813, p. 804-807
Effects of Gut Microbes on Nutrient Absorption and Energy Regulation
journal, February 2012
- Krajmalnik-Brown, Rosa; Ilhan, Zehra-Esra; Kang, Dae-Wook
- Nutrition in Clinical Practice, Vol. 27, Issue 2
Purification and characterization of recombinant Escherichia coli-expressed Pichia etchellsii ?-glucosidase II with high hydrolytic activity on sophorose
journal, November 2004
- Bhatia, Yukti; Mishra, Saroj; Bisaria, Virendra S.
- Applied Microbiology and Biotechnology, Vol. 66, Issue 5
Structure and function analysis of Pseudomonas plant cell wall hydrolases
journal, May 1998
- Hazlewood, G. P.; Gilbert, H. J.
- Biochemical Society Transactions, Vol. 26, Issue 2
Synthesis of three advanced biofuels from ionic liquid-pretreated switchgrass using engineered Escherichia coli
journal, November 2011
- Bokinsky, G.; Peralta-Yahya, P. P.; George, A.
- Proceedings of the National Academy of Sciences, Vol. 108, Issue 50, p. 19949-19954
The abundance and variety of carbohydrate-active enzymes in the human gut microbiota
journal, June 2013
- Kaoutari, Abdessamad El; Armougom, Fabrice; Gordon, Jeffrey I.
- Nature Reviews Microbiology, Vol. 11, Issue 7
Deconstruction of Lignocellulosic Biomass to Fuels and Chemicals
journal, July 2011
- Chundawat, Shishir P. S.; Beckham, Gregg T.; Himmel, Michael E.
- Annual Review of Chemical and Biomolecular Engineering, Vol. 2, Issue 1, p. 121-145
Structural Diversity and Function of Xyloglucan Sidechain Substituents
journal, November 2014
- Schultink, Alex; Liu, Lifeng; Zhu, Lei
- Plants, Vol. 3, Issue 4
The Devil Lies in the Details: How Variations in Polysaccharide Fine-Structure Impact the Physiology and Evolution of Gut Microbes
journal, November 2014
- Martens, Eric C.; Kelly, Amelia G.; Tauzin, Alexandra S.
- Journal of Molecular Biology, Vol. 426, Issue 23
Metagenomics and CAZyme Discovery
book, January 2017
- Kunath, Benoit J.; Bremges, Andreas; Weimann, Aaron
- Methods in Molecular Biology
Methods for the bioinformatic identification of bacterial lipoproteins encoded in the genomes of Gram-positive bacteria
journal, June 2008
- Rahman, Obaidur; Cummings, Stephen P.; Harrington, Dean J.
- World Journal of Microbiology and Biotechnology, Vol. 24, Issue 11
The emerging role for bacteria in lignin degradation and bio-product formation
journal, June 2011
- Bugg, Timothy DH; Ahmad, Mark; Hardiman, Elizabeth M.
- Current Opinion in Biotechnology, Vol. 22, Issue 3
Structural dissection of a complex Bacteroides ovatus gene locus conferring xyloglucan metabolism in the human gut
journal, July 2016
- Hemsworth, Glyn R.; Thompson, Andrew J.; Stepper, Judith
- Open Biology, Vol. 6, Issue 7
The Complete Genome Sequence of Escherichia coli K-12
journal, September 1997
- Blattner, F. R.
- Science, Vol. 277, Issue 5331
Structural and enzymatic characterization of a glycoside hydrolase family 31 α-xylosidase from Cellvibrio japonicus involved in xyloglucan saccharification
journal, May 2011
- Larsbrink, Johan; Izumi, Atsushi; Ibatullin, Farid M.
- Biochemical Journal, Vol. 436, Issue 3
Microbial Cellulose Utilization: Fundamentals and Biotechnology
journal, September 2002
- Lynd, L. R.; Weimer, P. J.; van Zyl, W. H.
- Microbiology and Molecular Biology Reviews, Vol. 66, Issue 3, p. 506-577
Generation and structural validation of a library of diverse xyloglucan-derived oligosaccharides, including an update on xyloglucan nomenclature
journal, January 2015
- Tuomivaara, Sami T.; Yaoi, Katsuro; O’Neill, Malcolm A.
- Carbohydrate Research, Vol. 402
Do Rumen Bacteroidetes Utilize an Alternative Mechanism for Cellulose Degradation?
journal, August 2014
- Naas, A. E.; Mackenzie, A. K.; Mravec, J.
- mBio, Vol. 5, Issue 4
The Thermal Degradation of Sugars I. Thermal Polymerization of Glucose
journal, July 1966
- Sugisawa, Hirqshi; Edo, Hiroshi
- Journal of Food Science, Vol. 31, Issue 4
Molecular domains of the cellulose/xyloglucan network in the cell walls of higher plants
journal, December 1999
- Pauly, Markus; Albersheim, Peter; Darvill, Alan
- The Plant Journal, Vol. 20, Issue 6
The carbohydrate-active enzymes database (CAZy) in 2013
journal, November 2013
- Lombard, Vincent; Golaconda Ramulu, Hemalatha; Drula, Elodie
- Nucleic Acids Research, Vol. 42, Issue D1
Structural and Functional Analysis of a Lytic Polysaccharide Monooxygenase Important for Efficient Utilization of Chitin in Cellvibrio japonicus
journal, February 2016
- Forsberg, Zarah; Nelson, Cassandra E.; Dalhus, Bjørn
- Journal of Biological Chemistry, Vol. 291, Issue 14
Synthesis, preliminary characterization, and application of novel surfactants from highly branched xyloglucan oligosaccharides
journal, November 2004
- Greffe, Lionel; Bessueille, Laurence; Bulone, Vincent
- Glycobiology, Vol. 15, Issue 4
Insights into Plant Cell Wall Degradation from the Genome Sequence of the Soil Bacterium Cellvibrio japonicus
journal, June 2008
- DeBoy, R. T.; Mongodin, E. F.; Fouts, D. E.
- Journal of Bacteriology, Vol. 190, Issue 15, p. 5455-5463
Signal Peptidases
journal, December 2002
- Paetzel, Mark; Karla, Andrew; Strynadka, Natalie C. J.
- Chemical Reviews, Vol. 102, Issue 12
Polysaccharide Utilization Loci: Fueling Microbial Communities
journal, January 2017
- Grondin, Julie M.; Tamura, Kazune; Déjean, Guillaume
- Journal of Bacteriology, Vol. 199, Issue 15
Architecture of the Yeast Cell Wall: β(1→6)-GLUCAN INTERCONNECTS MANNOPROTEIN, β(1→3)-GLUCAN, AND CHITIN
journal, July 1997
- Kollár, Roman; Reinhold, Bruce B.; Petráková, Eva
- Journal of Biological Chemistry, Vol. 272, Issue 28
Cyclic β-1,2-glucan is a brucella virulence factor required for intracellular survival
journal, May 2005
- Arellano-Reynoso, Beatriz; Lapaque, Nicolas; Salcedo, Susana
- Nature Immunology, Vol. 6, Issue 6
Glucose-tolerant β-glucosidase retrieved from a Kusaya gravy metagenome
journal, June 2015
- Uchiyama, Taku; Yaoi, Katusro; Miyazaki, Kentaro
- Frontiers in Microbiology, Vol. 6
Genome analyses highlight the different biological roles of cellulases
journal, January 2012
- Medie, Felix Mba; Davies, Gideon J.; Drancourt, Michel
- Nature Reviews Microbiology, Vol. 10, Issue 3
Comparative metabolism of cellulose, sophorose and glucose in Trichoderma reesei using high-throughput genomic and proteomic analyses
journal, January 2014
- dos Santos Castro, Lilian; Pedersoli, Wellington; Antoniêto, Amanda Cristina
- Biotechnology for Biofuels, Vol. 7, Issue 1
A discrete genetic locus confers xyloglucan metabolism in select human gut Bacteroidetes
journal, January 2014
- Larsbrink, Johan; Rogers, Theresa E.; Hemsworth, Glyn R.
- Nature, Vol. 506, Issue 7489
Genome sequence of Cellvibrio pealriver PR1, a xylanolytic and agarolytic bacterium isolated from freshwater
journal, November 2015
- Xie, Zhangzhang; Lin, Weitie; Luo, Jianfei
- Journal of Biotechnology, Vol. 214
Characterization of the gene celD and its encoded product 1,4-β-d-glucan glucohydrolase D from Pseudomonas fluorescens subsp. cellulosa
journal, August 1992
- Rixon, J. E.; Ferreira, L. M. A.; Durrant, A. J.
- Biochemical Journal, Vol. 285, Issue 3
Systems biology defines the biological significance of redox‐active proteins during cellulose degradation in an aerobic bacterium
journal, October 2014
- Gardner, Jeffrey G.; Crouch, Lucy; Labourel, Aurore
- Molecular Microbiology, Vol. 94, Issue 5
Requirement of the Type II Secretion System for Utilization of Cellulosic Substrates by Cellvibrio japonicus
journal, June 2010
- Gardner, J. G.; Keating, D. H.
- Applied and Environmental Microbiology, Vol. 76, Issue 15
NMR Spectroscopic Analysis Reveals Extensive Binding Interactions of Complex Xyloglucan Oligosaccharides with the Cellvibrio japonicus Glycoside Hydrolase Family 31 α-Xylosidase
journal, September 2012
- Silipo, Alba; Larsbrink, Johan; Marchetti, Roberta
- Chemistry - A European Journal, Vol. 18, Issue 42
New-to-nature sophorose analog: a potent inducer for gene expression in Trichoderma reesei
journal, April 2016
- Huang, Tom Tao; Wages, John M.
- Enzyme and Microbial Technology, Vol. 85
Biology of callose (β-1,3-glucan) turnover at plasmodesmata
journal, November 2010
- Zavaliev, Raul; Ueki, Shoko; Epel, Bernard L.
- Protoplasma, Vol. 248, Issue 1
Polysaccharide degradation systems of the saprophytic bacterium Cellvibrio japonicus
journal, June 2016
- Gardner, Jeffrey G.
- World Journal of Microbiology and Biotechnology, Vol. 32, Issue 7
Plant cell walls to ethanol
journal, February 2012
- Jordan, Douglas B.; Bowman, Michael J.; Braker, Jay D.
- Biochemical Journal, Vol. 442, Issue 2
Custom fabrication of biomass containment devices using 3-D printing enables bacterial growth analyses with complex insoluble substrates
journal, November 2016
- Nelson, Cassandra E.; Beri, Nina R.; Gardner, Jeffrey G.
- Journal of Microbiological Methods, Vol. 130
An alternative polysaccharide uptake mechanism of marine bacteria
journal, March 2017
- Reintjes, Greta; Arnosti, Carol; Fuchs, Bernhard M.
- The ISME Journal, Vol. 11, Issue 7
Unique aspects of the grass cell wall
journal, June 2008
- Vogel, J.
- Current Opinion in Plant Biology, Vol. 11, Issue 3
Culture Medium for Enterobacteria
journal, January 1974
- Neidhardt, Frederick C.; Bloch, Philip L.; Smith, David F.
- Journal of Bacteriology, Vol. 119, Issue 3
An alternative polysaccharide uptake mechanism of marine bacteria
text, January 2017
- Rudolf, Amann,; Greta, Reintjes,; M., Fuchs, Bernhard
- The University of North Carolina at Chapel Hill University Libraries
Signal Peptidases
journal, February 2003
- Paetzel, Mark; Karla, Andrew; Strynadka, Natalie C. J.
- ChemInform, Vol. 34, Issue 6
Works referencing / citing this record:
Identification and characterization of α-xylosidase involved in xyloglucan degradation in Aspergillus oryzae
journal, November 2019
- Matsuzawa, Tomohiko; Kameyama, Akihiko; Yaoi, Katsuro
- Applied Microbiology and Biotechnology, Vol. 104, Issue 1
Laminarin is a major molecule in the marine carbon cycle
journal, March 2020
- Becker, Stefan; Tebben, Jan; Coffinet, Sarah
- Proceedings of the National Academy of Sciences, Vol. 117, Issue 12
Substrate specificity, regiospecificity, and processivity in glycoside hydrolase family 74
journal, July 2019
- Arnal, Gregory; Stogios, Peter J.; Asohan, Jathavan
- Journal of Biological Chemistry, Vol. 294, Issue 36
Molecular recognition of the beta‐glucans laminarin and pustulan by a SusD‐like glycan‐binding protein of a marine Bacteroidetes
journal, October 2018
- Mystkowska, Agata Anna; Robb, Craig; Vidal‐Melgosa, Silvia
- The FEBS Journal, Vol. 285, Issue 23
Complete Genome Sequences of Cellvibrio japonicus Strains with Improved Growth When Using α-Diglucosides
journal, October 2019
- Garcia, Cecelia A.; Narrett, Jackson A.; Gardner, Jeffrey G.
- Microbiology Resource Announcements, Vol. 8, Issue 44
In vitro and in vivo characterization of three Cellvibrio japonicus glycoside hydrolase family 5 members reveals potent xyloglucan backbone-cleaving functions
journal, February 2018
- Attia, Mohamed A.; Nelson, Cassandra E.; Offen, Wendy A.
- Biotechnology for Biofuels, Vol. 11, Issue 1
Plant–necrotroph co-transcriptome networks illuminate a metabolic battlefield
journal, May 2019
- Zhang, Wei; Corwin, Jason A.; Copeland, Daniel Harrison
- eLife, Vol. 8
In vitro and in vivo characterization of three Cellvibrio japonicus glycoside hydrolase family 5 members reveals potent xyloglucan backbone-cleaving functions
text, January 2018
- Attia, Mohamed A.; Nelson, Cassandra E.; Offen, Wendy A.
- Maryland Shared Open Access Repository
In vitro and in vivo characterization of three Cellvibrio japonicus glycoside hydrolase family 5 members reveals potent xyloglucan backbone-cleaving functions
text, January 2018
- Attia, Mohamed A.; Nelson, Cassandra E.; Offen, Wendy A.
- BioMed Central
Molecular recognition of the beta-glucans laminarin and pustulan by a SusD-like glycan-binding protein of a marine Bacteroidetes
text, January 2018
- Mystkowska, Agata Anna; Robb, Craig; Vidal-Melgosa, Silvia
- Deutsches Elektronen-Synchrotron, DESY, Hamburg
Complete Genome Sequences of Cellvibrio japonicus Strains with Improved Growth When Using α-Diglucosides
journal, October 2019
- Garcia, Cecelia A.; Narrett, Jackson A.; Gardner, Jeffrey G.
- Microbiology Resource Announcements, Vol. 8, Issue 44
In vitro and in vivo characterization of three Cellvibrio japonicus glycoside hydrolase family 5 members reveals potent xyloglucan backbone-cleaving functions
journal, February 2018
- Attia, Mohamed A.; Nelson, Cassandra E.; Offen, Wendy A.
- Biotechnology for Biofuels, Vol. 11, Issue 1
Plant–necrotroph co-transcriptome networks illuminate a metabolic battlefield
journal, May 2019
- Zhang, Wei; Corwin, Jason A.; Copeland, Daniel Harrison
- eLife, Vol. 8
Figures / Tables found in this record:
Figures/Tables have been extracted from DOE-funded journal article accepted manuscripts.