Determination of the Complete Spin Density Distribution in 13 C-Labeled Protein-Bound Radical Intermediates Using Advanced 2D Electron Paramagnetic Resonance Spectroscopy and Density Functional Theory
Abstract
Determining the complete electron spin density distribution for protein-bound radicals, even with advanced pulsed electron paramagnetic resonance (EPR) methods, is a formidable task. Here we present a strategy to overcome this problem combining multifrequency HYSCORE and ENDOR measurements on site-specifically 13C-labeled samples with DFT calculations on model systems. As a demonstration of this approach, pulsed EPR experiments are performed on the primary QA and secondary QB ubisemiquinones of the photosynthetic reaction center from Rhodobacter sphaeroides 13C-labeled at the ring and tail positions. Despite the large number of nuclei interacting with the unpaired electron in these samples, two-dimensional X- and Q-band HYSCORE and orientation selective Q-band ENDOR resolve and allow for a characterization of the eight expected 13C resonances from significantly different hyperfine tensors for both semiquinones. From these results we construct, for the first time, the most complete experimentally determined maps of the s- and pπ-orbital spin density distributions for any protein organic cofactor radical to date. This work lays a foundation for understanding the relationship between the electronic structure of semiquinones and their functional properties, and introduces new techniques for mapping out the spin density distribution that are readily applicable to other systems.
- Authors:
-
[1];
- Center for Biophysics and Computational Biology, University of Illinois at Urbana−Champaign, Urbana, Illinois 61801, United States, Department of Veterinary Clinical Medicine, University of Illinois at Urbana−Champaign, Urbana, Illinois 61801, United States
- School of Chemistry, The University of Manchester, Manchester M13 9PL, U.K.
- Center for Biophysics and Computational Biology, University of Illinois at Urbana−Champaign, Urbana, Illinois 61801, United States, Department of Biochemistry, University of Illinois at Urbana−Champaign, Urbana, Illinois 61801, United States
- Department of Veterinary Clinical Medicine, University of Illinois at Urbana−Champaign, Urbana, Illinois 61801, United States
- Publication Date:
- Research Org.:
- Univ. of Illinois at Urbana−Champaign, Urbana, IL (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES)
- OSTI Identifier:
- 1406160
- Alternate Identifier(s):
- OSTI ID: 1508078
- Grant/Contract Number:
- FG02-08ER15960
- Resource Type:
- Published Article
- Journal Name:
- Journal of Physical Chemistry. B
- Additional Journal Information:
- Journal Name: Journal of Physical Chemistry. B Journal Volume: 121 Journal Issue: 44; Journal ID: ISSN 1520-6106
- Publisher:
- American Chemical Society
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
Citation Formats
Taguchi, Alexander T., O’Malley, Patrick J., Wraight, Colin A., and Dikanov, Sergei A. Determination of the Complete Spin Density Distribution in 13 C-Labeled Protein-Bound Radical Intermediates Using Advanced 2D Electron Paramagnetic Resonance Spectroscopy and Density Functional Theory. United States: N. p., 2017.
Web. doi:10.1021/acs.jpcb.7b10036.
Taguchi, Alexander T., O’Malley, Patrick J., Wraight, Colin A., & Dikanov, Sergei A. Determination of the Complete Spin Density Distribution in 13 C-Labeled Protein-Bound Radical Intermediates Using Advanced 2D Electron Paramagnetic Resonance Spectroscopy and Density Functional Theory. United States. https://doi.org/10.1021/acs.jpcb.7b10036
Taguchi, Alexander T., O’Malley, Patrick J., Wraight, Colin A., and Dikanov, Sergei A. Tue .
"Determination of the Complete Spin Density Distribution in 13 C-Labeled Protein-Bound Radical Intermediates Using Advanced 2D Electron Paramagnetic Resonance Spectroscopy and Density Functional Theory". United States. https://doi.org/10.1021/acs.jpcb.7b10036.
@article{osti_1406160,
title = {Determination of the Complete Spin Density Distribution in 13 C-Labeled Protein-Bound Radical Intermediates Using Advanced 2D Electron Paramagnetic Resonance Spectroscopy and Density Functional Theory},
author = {Taguchi, Alexander T. and O’Malley, Patrick J. and Wraight, Colin A. and Dikanov, Sergei A.},
abstractNote = {Determining the complete electron spin density distribution for protein-bound radicals, even with advanced pulsed electron paramagnetic resonance (EPR) methods, is a formidable task. Here we present a strategy to overcome this problem combining multifrequency HYSCORE and ENDOR measurements on site-specifically 13C-labeled samples with DFT calculations on model systems. As a demonstration of this approach, pulsed EPR experiments are performed on the primary QA and secondary QB ubisemiquinones of the photosynthetic reaction center from Rhodobacter sphaeroides 13C-labeled at the ring and tail positions. Despite the large number of nuclei interacting with the unpaired electron in these samples, two-dimensional X- and Q-band HYSCORE and orientation selective Q-band ENDOR resolve and allow for a characterization of the eight expected 13C resonances from significantly different hyperfine tensors for both semiquinones. From these results we construct, for the first time, the most complete experimentally determined maps of the s- and pπ-orbital spin density distributions for any protein organic cofactor radical to date. This work lays a foundation for understanding the relationship between the electronic structure of semiquinones and their functional properties, and introduces new techniques for mapping out the spin density distribution that are readily applicable to other systems.},
doi = {10.1021/acs.jpcb.7b10036},
journal = {Journal of Physical Chemistry. B},
number = 44,
volume = 121,
place = {United States},
year = {Tue Oct 31 00:00:00 EDT 2017},
month = {Tue Oct 31 00:00:00 EDT 2017}
}
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https://doi.org/10.1021/acs.jpcb.7b10036
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Cited by: 10 works
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Figures / Tables:
Figure 1: Symmetric locations of the QA and QB sites about the nonheme iron (green) in pdb entry 1DV3. The quinone ring carbons are numbered as referenced in the text, and only the first (CH2) and second (CH) carbons of the isoprenoid tails are shown extending from the C6 position.more »
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