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Title: BBMerge – Accurate paired shotgun read merging via overlap

Merging paired-end shotgun reads generated on high-throughput sequencing platforms can substantially improve various subsequent bioinformatics processes, including genome assembly, binning, mapping, annotation, and clustering for taxonomic analysis. With the inexorable growth of sequence data volume and CPU core counts, the speed and scalability of read-processing tools becomes ever-more important. The accuracy of shotgun read merging is crucial as well, as errors introduced by incorrect merging percolate through to reduce the quality of downstream analysis. Thus, we designed a new tool to maximize accuracy and minimize processing time, allowing the use of read merging on larger datasets, and in analyses highly sensitive to errors. We present BBMerge, a new merging tool for paired-end shotgun sequence data. We benchmark BBMerge by comparison with eight other widely used merging tools, assessing speed, accuracy and scalability. Evaluations of both synthetic and real-world datasets demonstrate that BBMerge produces merged shotgun reads with greater accuracy and at higher speed than any existing merging tool examined. BBMerge also provides the ability to merge non-overlapping shotgun read pairs by using k-mer frequency information to assemble the unsequenced gap between reads, achieving a significantly higher merge rate while maintaining or increasing accuracy.
 [1] ;  [2] ; ORCiD logo [1]
  1. USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States)
  2. National Renewable Energy Lab. (NREL), Golden, CO (United States)
Publication Date:
Report Number(s):
Journal ID: ISSN 1932-6203
Grant/Contract Number:
AC02-05CH11231; AC36-08GO28308
Published Article
Journal Name:
Additional Journal Information:
Journal Volume: 12; Journal Issue: 10; Journal ID: ISSN 1932-6203
Public Library of Science
Research Org:
USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States); National Renewable Energy Lab. (NREL), Golden, CO (United States)
Sponsoring Org:
USDOE Office of Science (SC)
Country of Publication:
United States
59 BASIC BIOLOGICAL SCIENCES; 97 MATHEMATICS AND COMPUTING; metagenomics; sequence alignment; shotgun sequencing; sequence assembly tools; computer software; bacteria; genome annotation
OSTI Identifier:
Alternate Identifier(s):
OSTI ID: 1407458