skip to main content
DOE PAGES title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Engineering oxidative stress defense pathways to build a robust lipid production platform in Yarrowia lipolytica: Engineering robust microbial oil cell factory

Authors:
ORCiD logo [1];  [2];  [2]
  1. Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge Massachusetts 02139, Department of Chemical, Biochemical and Environmental Engineering, University of Maryland, Baltimore County, Baltimore Maryland
  2. Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge Massachusetts 02139
Publication Date:
Sponsoring Org.:
USDOE
OSTI Identifier:
1401898
Grant/Contract Number:  
SC0008744
Resource Type:
Publisher's Accepted Manuscript
Journal Name:
Biotechnology and Bioengineering
Additional Journal Information:
Journal Name: Biotechnology and Bioengineering Journal Volume: 114 Journal Issue: 7; Journal ID: ISSN 0006-3592
Publisher:
Wiley Blackwell (John Wiley & Sons)
Country of Publication:
United States
Language:
English

Citation Formats

Xu, Peng, Qiao, Kangjian, and Stephanopoulos, Gregory. Engineering oxidative stress defense pathways to build a robust lipid production platform in Yarrowia lipolytica: Engineering robust microbial oil cell factory. United States: N. p., 2017. Web. doi:10.1002/bit.26285.
Xu, Peng, Qiao, Kangjian, & Stephanopoulos, Gregory. Engineering oxidative stress defense pathways to build a robust lipid production platform in Yarrowia lipolytica: Engineering robust microbial oil cell factory. United States. doi:10.1002/bit.26285.
Xu, Peng, Qiao, Kangjian, and Stephanopoulos, Gregory. Tue . "Engineering oxidative stress defense pathways to build a robust lipid production platform in Yarrowia lipolytica: Engineering robust microbial oil cell factory". United States. doi:10.1002/bit.26285.
@article{osti_1401898,
title = {Engineering oxidative stress defense pathways to build a robust lipid production platform in Yarrowia lipolytica: Engineering robust microbial oil cell factory},
author = {Xu, Peng and Qiao, Kangjian and Stephanopoulos, Gregory},
abstractNote = {},
doi = {10.1002/bit.26285},
journal = {Biotechnology and Bioengineering},
number = 7,
volume = 114,
place = {United States},
year = {2017},
month = {4}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
DOI: 10.1002/bit.26285

Citation Metrics:
Cited by: 16 works
Citation information provided by
Web of Science

Save / Share:

Works referenced in this record:

Oxidative Stress Is a Mediator for Increased Lipid Accumulation in a Newly Isolated Dunaliella salina Strain
journal, March 2014


Engineering Yarrowia lipolytica as a platform for synthesis of drop-in transportation fuels and oleochemicals
journal, September 2016

  • Xu, Peng; Qiao, Kangjian; Ahn, Woo Suk
  • Proceedings of the National Academy of Sciences, Vol. 113, Issue 39
  • DOI: 10.1073/pnas.1607295113

Fatty Acid-Derived Biofuels and Chemicals Production in Saccharomyces cerevisiae
journal, September 2014

  • Zhou, Yongjin J.; Buijs, Nicolaas A.; Siewers, Verena
  • Frontiers in Bioengineering and Biotechnology, Vol. 2
  • DOI: 10.3389/fbioe.2014.00032

Metabolic engineering strategies for microbial synthesis of oleochemicals
journal, May 2015


Overexpression of the active diacylglycerol acyltransferase variant transforms Saccharomyces cerevisiae into an oleaginous yeast
journal, April 2013

  • Kamisaka, Yasushi; Kimura, Kazuyoshi; Uemura, Hiroshi
  • Applied Microbiology and Biotechnology, Vol. 97, Issue 16
  • DOI: 10.1007/s00253-013-4915-9

Improving fatty acids production by engineering dynamic pathway regulation and metabolic control
journal, July 2014

  • Xu, P.; Li, L.; Zhang, F.
  • Proceedings of the National Academy of Sciences, Vol. 111, Issue 31
  • DOI: 10.1073/pnas.1406401111

Engineered reversal of the β-oxidation cycle for the synthesis of fuels and chemicals
journal, August 2011

  • Dellomonaco, Clementina; Clomburg, James M.; Miller, Elliot N.
  • Nature, Vol. 476, Issue 7360, p. 355-359
  • DOI: 10.1038/nature10333

Biodiesel and renewable diesel: A comparison
journal, June 2010


Modular optimization of multi-gene pathways for fatty acids production in E. coli
journal, January 2013

  • Xu, Peng; Gu, Qin; Wang, Wenya
  • Nature Communications, Vol. 4, Issue 1
  • DOI: 10.1038/ncomms2425

The NADPH:Quinone Oxidoreductase P1-ζ-crystallin in Arabidopsis Catalyzes the α,β-Hydrogenation of 2-Alkenals: Detoxication of the Lipid Peroxide-Derived Reactive Aldehydes
journal, December 2002

  • Mano, Jun’ichi; Torii, Yoshimitsu; Hayashi, Shun-ichiro
  • Plant and Cell Physiology, Vol. 43, Issue 12
  • DOI: 10.1093/pcp/pcf187

Engineered biosynthesis of medium-chain esters in Escherichia coli
journal, January 2015


Redox control of catalysis in ATP-citrate lyase from rat liver
journal, February 1992


The Response to Heat Shock and Oxidative Stress in Saccharomyces cerevisiae
journal, December 2011


Hexokinase—A limiting factor in lipid production from fructose in Yarrowia lipolytica
journal, November 2014


Mannitol Protects against Oxidation by Hydroxyl Radicals
journal, October 1997

  • Shen, B.; Jensen, R. G.; Bohnert, H. J.
  • Plant Physiology, Vol. 115, Issue 2
  • DOI: 10.1104/pp.115.2.527

Redox regulation of maize NADP-malic enzyme by thiol-disulfide interchange: effect of reduced thioredoxin on activity
journal, May 1994

  • Drincovich, María F.; Andreo, Carlos S.
  • Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, Vol. 1206, Issue 1
  • DOI: 10.1016/0167-4838(94)90066-3

Metabolic engineering of Escherichia coli for biofuel production
journal, May 2010


Engineering the push and pull of lipid biosynthesis in oleaginous yeast Yarrowia lipolytica for biofuel production
journal, January 2013


An evolutionary metabolic engineering approach for enhancing lipogenesis in Yarrowia lipolytica
journal, May 2015


Yarrowia lipolytica
journal, October 2012


Engineering lipid overproduction in the oleaginous yeast Yarrowia lipolytica
journal, May 2015


The oxidative pentose phosphate pathway is the primary source of NADPH for lipid overproduction from glucose in Yarrowia lipolytica
journal, July 2015


Increased Resistance to Oxidative Stress in Transgenic Plants by Targeting Mannitol Biosynthesis to Chloroplasts
journal, April 1997


SOD1 Integrates Signals from Oxygen and Glucose to Repress Respiration
journal, January 2013


Link between light and fatty acid synthesis: Thioredoxin-linked reductive activation of plastidic acetyl-CoA carboxylase
journal, September 1997

  • Sasaki, Y.; Kozaki, A.; Hatano, M.
  • Proceedings of the National Academy of Sciences, Vol. 94, Issue 20
  • DOI: 10.1073/pnas.94.20.11096

Sustainable source of omega-3 eicosapentaenoic acid from metabolically engineered Yarrowia lipolytica: from fundamental research to commercial production
journal, January 2015

  • Xie, Dongming; Jackson, Ethel N.; Zhu, Quinn
  • Applied Microbiology and Biotechnology, Vol. 99, Issue 4
  • DOI: 10.1007/s00253-014-6318-y

Activation of the Alternative Oxidase of Yarrowia lipolytica by Adenosine Monophosphate
journal, March 2004


Lipid Accumulation, Lipid Body Formation, and Acyl Coenzyme A Oxidases of the Yeast Yarrowia lipolytica
journal, July 2004


Mapping the diatom redox-sensitive proteome provides insight into response to nitrogen stress in the marine environment
journal, February 2014

  • Rosenwasser, S.; Graff van Creveld, S.; Schatz, D.
  • Proceedings of the National Academy of Sciences, Vol. 111, Issue 7
  • DOI: 10.1073/pnas.1319773111

Oxidative stress in fungal fermentation processes: the roles of alternative respiration
journal, November 2010


α-Ketoglutarate dehydrogenase: A mitochondrial redox sensor
journal, November 2010


Engineering alcohol tolerance in yeast
journal, October 2014


Microbial production of fatty acid-derived fuels and chemicals
journal, December 2013


Mouse aldehyde dehydrogenase ALDH3B2 is localized to lipid droplets via two C-terminal tryptophan residues and lipid modification
journal, December 2014

  • Kitamura, Takuya; Takagi, Shuyu; Naganuma, Tatsuro
  • Biochemical Journal, Vol. 465, Issue 1
  • DOI: 10.1042/BJ20140624

Exploiting nongenetic cell-to-cell variation for enhanced biosynthesis
journal, March 2016

  • Xiao, Yi; Bowen, Christopher H.; Liu, Di
  • Nature Chemical Biology, Vol. 12, Issue 5
  • DOI: 10.1038/nchembio.2046

ePathBrick: A Synthetic Biology Platform for Engineering Metabolic Pathways in E. coli
journal, May 2012

  • Xu, Peng; Vansiri, Amerin; Bhan, Namita
  • ACS Synthetic Biology, Vol. 1, Issue 7
  • DOI: 10.1021/sb300016b

Transcriptomic Analyses during the Transition from Biomass Production to Lipid Accumulation in the Oleaginous Yeast Yarrowia lipolytica
journal, November 2011


One-step transformation of the dimorphic yeast Yarrowia lipolytica
journal, August 1997

  • Chen, D.-C.; Beckerich, J.-M.; Gaillardin, C.
  • Applied Microbiology and Biotechnology, Vol. 48, Issue 2, p. 232-235
  • DOI: 10.1007/s002530051043

Acyl-Lipid Metabolism
journal, January 2010

  • Li-Beisson, Yonghua; Shorrosh, Basil; Beisson, Fred
  • The Arabidopsis Book, Vol. 8
  • DOI: 10.1199/tab.0133

Mitochondria and cancer
journal, September 2012

  • Wallace, Douglas C.
  • Nature Reviews Cancer, Vol. 12, Issue 10
  • DOI: 10.1038/nrc3365

Oxidative Stress and Covalent Modification of Protein with Bioactive Aldehydes
journal, April 2008

  • Grimsrud, Paul A.; Xie, Hongwei; Griffin, Timothy J.
  • Journal of Biological Chemistry, Vol. 283, Issue 32
  • DOI: 10.1074/jbc.R700019200

Heterologous production of pentane in the oleaginous yeast Yarrowia lipolytica
journal, June 2013


Lipid production in Yarrowia lipolytica is maximized by engineering cytosolic redox metabolism
journal, January 2017

  • Qiao, Kangjian; Wasylenko, Thomas M.; Zhou, Kang
  • Nature Biotechnology, Vol. 35, Issue 2
  • DOI: 10.1038/nbt.3763

Production of omega-3 eicosapentaenoic acid by metabolic engineering of Yarrowia lipolytica
journal, July 2013

  • Xue, Zhixiong; Sharpe, Pamela L.; Hong, Seung-Pyo
  • Nature Biotechnology, Vol. 31, Issue 8
  • DOI: 10.1038/nbt.2622

Metabolic engineering of Saccharomyces cerevisiae for production of fatty acid-derived biofuels and chemicals
journal, January 2014


Mannitol Is Required for Stress Tolerance in Aspergillus niger Conidiospores
journal, August 2003


Comparison of Yarrowia lipolytica and Pichia pastoris Cellular Response to Different Agents of Oxidative Stress
journal, April 2013

  • Lopes, Marlene; Mota, Manuel; Belo, Isabel
  • Applied Biochemistry and Biotechnology, Vol. 170, Issue 2
  • DOI: 10.1007/s12010-013-0205-3

Engineering of chromosomal wax ester synthase integrated Saccharomyces cerevisiae mutants for improved biosynthesis of fatty acid ethyl esters : Enhancement of FAEEs Production
journal, April 2014

  • Shi, Shuobo; Valle-Rodríguez, Juan Octavio; Siewers, Verena
  • Biotechnology and Bioengineering, Vol. 111, Issue 9
  • DOI: 10.1002/bit.25234

Metabolic Engineering for Stress Tolerance: Installing Osmoprotectant Synthesis Pathways
journal, October 2000


Sources of NADPH and Expression of Mammalian NADP + -specific Isocitrate Dehydrogenases in Saccharomyces cerevisiae
journal, November 1998

  • Minard, Karyl I.; Jennings, Gary T.; Loftus, Thomas M.
  • Journal of Biological Chemistry, Vol. 273, Issue 47
  • DOI: 10.1074/jbc.273.47.31486

In Yarrowia lipolytica mitochondria, the alternative NADH dehydrogenase interacts specifically with the cytochrome complexes of the classic respiratory pathway
journal, February 2009

  • Guerrero-Castillo, Sergio; Vázquez-Acevedo, Miriam; González-Halphen, Diego
  • Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1787, Issue 2
  • DOI: 10.1016/j.bbabio.2008.10.008

Acidosis induces reprogramming of cellular metabolism to mitigate oxidative stress
journal, January 2013

  • LaMonte, Gregory; Tang, Xiaohu; Chen, Julia Ling-Yu
  • Cancer & Metabolism, Vol. 1, Issue 1
  • DOI: 10.1186/2049-3002-1-23

Control of Lipid Accumulation in the Yeast Yarrowia lipolytica
journal, October 2008

  • Beopoulos, A.; Mrozova, Z.; Thevenieau, F.
  • Applied and Environmental Microbiology, Vol. 74, Issue 24, p. 7779-7789
  • DOI: 10.1128/AEM.01412-08

Harnessing Yarrowia lipolytica lipogenesis to create a platform for lipid and biofuel production
journal, January 2014

  • Blazeck, John; Hill, Andrew; Liu, Leqian
  • Nature Communications, Vol. 5, Issue 1
  • DOI: 10.1038/ncomms4131

Relationship of electrophilic stress to aging
journal, September 2011


Fusing catalase to an alkane-producing enzyme maintains enzymatic activity by converting the inhibitory byproduct H2O2 to the cosubstrate O2
journal, February 2013

  • Andre, C.; Kim, S. W.; Yu, X. -H.
  • Proceedings of the National Academy of Sciences, Vol. 110, Issue 8
  • DOI: 10.1073/pnas.1218769110