Identification and overexpression of gibberellin 2‐oxidase ( GA 2ox ) in switchgrass ( P anicum virgatum L.) for improved plant architecture and reduced biomass recalcitrance
Abstract
Summary Gibberellin 2‐oxidases ( GA 2oxs) are a group of 2‐oxoglutarate‐dependent dioxygenases that catalyse the deactivation of bioactive GA or its precursors through 2β‐hydroxylation reaction. In this study, putatively novel switchgrass C 20 GA 2ox genes were identified with the aim of genetically engineering switchgrass for improved architecture and reduced biomass recalcitrance for biofuel. Three C 20 GA 2ox genes showed differential regulation patterns among tissues including roots, seedlings and reproductive parts. Using a transgenic approach, we showed that overexpression of two C20 GA2ox genes, that is PvGA2ox5 and PvGA2ox9 , resulted in characteristic GA‐deficient phenotypes with dark‐green leaves and modified plant architecture. The changes in plant morphology appeared to be associated with GA2ox transcript abundance. Exogenous application of GA rescued the GA ‐deficient phenotypes in transgenic lines. Transgenic semi‐dwarf lines displayed increased tillering and reduced lignin content, and the syringyl/guaiacyl lignin monomer ratio accompanied by the reduced expression of lignin biosynthetic genes compared to nontransgenic plants. A moderate increase in the level of glucose release in these transgenic lines might be attributed to reduced biomass recalcitrance as a result of reduced lignin content and lignin composition. Our results suggest that overexpression of GA 2ox genes in switchgrass is amore »
- Authors:
-
- Department of Plant Sciences University of Tennessee Knoxville TN 37996 USA, Bioenergy Science Center Oak Ridge National Laboratory Oak Ridge TN 37831 USA
- Bioenergy Science Center Oak Ridge National Laboratory Oak Ridge TN 37831 USA, Plant Biology Division Samuel Roberts Noble Foundation Ardmore OK 73401 USA
- Bioenergy Science Center Oak Ridge National Laboratory Oak Ridge TN 37831 USA, National Renewable Energy Laboratory Golden CO 80401 USA
- Publication Date:
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1401831
- Resource Type:
- Publisher's Accepted Manuscript
- Journal Name:
- Plant Biotechnology Journal
- Additional Journal Information:
- Journal Name: Plant Biotechnology Journal Journal Volume: 13 Journal Issue: 5; Journal ID: ISSN 1467-7644
- Publisher:
- Wiley-Blackwell
- Country of Publication:
- United Kingdom
- Language:
- English
Citation Formats
Wuddineh, Wegi A., Mazarei, Mitra, Zhang, Jiyi, Poovaiah, Charleson R., Mann, David G. J., Ziebell, Angela, Sykes, Robert W., Davis, Mark F., Udvardi, Michael K., and Stewart, Jr, Charles Neal. Identification and overexpression of gibberellin 2‐oxidase ( GA 2ox ) in switchgrass ( P anicum virgatum L.) for improved plant architecture and reduced biomass recalcitrance. United Kingdom: N. p., 2014.
Web. doi:10.1111/pbi.12287.
Wuddineh, Wegi A., Mazarei, Mitra, Zhang, Jiyi, Poovaiah, Charleson R., Mann, David G. J., Ziebell, Angela, Sykes, Robert W., Davis, Mark F., Udvardi, Michael K., & Stewart, Jr, Charles Neal. Identification and overexpression of gibberellin 2‐oxidase ( GA 2ox ) in switchgrass ( P anicum virgatum L.) for improved plant architecture and reduced biomass recalcitrance. United Kingdom. https://doi.org/10.1111/pbi.12287
Wuddineh, Wegi A., Mazarei, Mitra, Zhang, Jiyi, Poovaiah, Charleson R., Mann, David G. J., Ziebell, Angela, Sykes, Robert W., Davis, Mark F., Udvardi, Michael K., and Stewart, Jr, Charles Neal. Fri .
"Identification and overexpression of gibberellin 2‐oxidase ( GA 2ox ) in switchgrass ( P anicum virgatum L.) for improved plant architecture and reduced biomass recalcitrance". United Kingdom. https://doi.org/10.1111/pbi.12287.
@article{osti_1401831,
title = {Identification and overexpression of gibberellin 2‐oxidase ( GA 2ox ) in switchgrass ( P anicum virgatum L.) for improved plant architecture and reduced biomass recalcitrance},
author = {Wuddineh, Wegi A. and Mazarei, Mitra and Zhang, Jiyi and Poovaiah, Charleson R. and Mann, David G. J. and Ziebell, Angela and Sykes, Robert W. and Davis, Mark F. and Udvardi, Michael K. and Stewart, Jr, Charles Neal},
abstractNote = {Summary Gibberellin 2‐oxidases ( GA 2oxs) are a group of 2‐oxoglutarate‐dependent dioxygenases that catalyse the deactivation of bioactive GA or its precursors through 2β‐hydroxylation reaction. In this study, putatively novel switchgrass C 20 GA 2ox genes were identified with the aim of genetically engineering switchgrass for improved architecture and reduced biomass recalcitrance for biofuel. Three C 20 GA 2ox genes showed differential regulation patterns among tissues including roots, seedlings and reproductive parts. Using a transgenic approach, we showed that overexpression of two C20 GA2ox genes, that is PvGA2ox5 and PvGA2ox9 , resulted in characteristic GA‐deficient phenotypes with dark‐green leaves and modified plant architecture. The changes in plant morphology appeared to be associated with GA2ox transcript abundance. Exogenous application of GA rescued the GA ‐deficient phenotypes in transgenic lines. Transgenic semi‐dwarf lines displayed increased tillering and reduced lignin content, and the syringyl/guaiacyl lignin monomer ratio accompanied by the reduced expression of lignin biosynthetic genes compared to nontransgenic plants. A moderate increase in the level of glucose release in these transgenic lines might be attributed to reduced biomass recalcitrance as a result of reduced lignin content and lignin composition. Our results suggest that overexpression of GA 2ox genes in switchgrass is a feasible strategy to improve plant architecture and reduce biomass recalcitrance for biofuel.},
doi = {10.1111/pbi.12287},
journal = {Plant Biotechnology Journal},
number = 5,
volume = 13,
place = {United Kingdom},
year = {Fri Nov 14 00:00:00 EST 2014},
month = {Fri Nov 14 00:00:00 EST 2014}
}
https://doi.org/10.1111/pbi.12287
Web of Science
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