Crystallographic insight into the evolutionary origins of xyloglucan endotransglycosylases and endohydrolases
Abstract
Summary The xyloglucan endotransglycosylase/hydrolase ( XTH ) gene family encodes enzymes of central importance to plant cell wall remodeling. The evolutionary history of plant XTH gene products is incompletely understood vis‐à‐vis the larger body of bacterial endoglycanases in Glycoside Hydrolase Family 16 ( GH 16). To provide molecular insight into this issue, high‐resolution X‐ray crystal structures and detailed enzyme kinetics of an extant transitional plant endoglucanase ( EG ) were determined. Functionally intermediate between plant XTH gene products and bacterial licheninases of GH 16, Vitis vinifera EG 16 (Vv EG 16) effectively catalyzes the hydrolysis of the backbones of two dominant plant cell wall matrix glycans, xyloglucan (XyG) and β(1,3)/β(1,4)‐mixed‐linkage glucan ( MLG ). Crystallographic complexes with extended oligosaccharide substrates reveal the structural basis for the accommodation of both unbranched, mixed‐linked ( MLG ) and highly decorated, linear (XyG) polysaccharide chains in a broad, extended active‐site cleft. Structural comparison with representative bacterial licheninases, a xyloglucan endotranglycosylase ( XET ), and a xyloglucan endohydrolase ( XEH ) outline the functional ramifications of key sequence deletions and insertions across the phylogenetic landscape of GH 16. Although the biological role(s) of EG 16 orthologs remains to be fully resolved, the present biochemical andmore »
- Authors:
-
- Michael Smith Laboratories University of British Columbia 2185 East Mall Vancouver BC V6T 1Z4 Canada, Department of Chemistry University of British Columbia 2036 Main Mall Vancouver BC V6T 1Z1 Canada
- Department of Biochemistry and Molecular Biology University of British Columbia 2350 Health Sciences Mall Vancouver BC V6T 1Z3 Canada
- Michael Smith Laboratories University of British Columbia 2185 East Mall Vancouver BC V6T 1Z4 Canada, Department of Chemistry University of British Columbia 2036 Main Mall Vancouver BC V6T 1Z1 Canada, Department of Biochemistry and Molecular Biology University of British Columbia 2350 Health Sciences Mall Vancouver BC V6T 1Z3 Canada, Department of Botany University of British Columbia 6270 University Boulevard Vancouver BC V6T 1Z4 Canada
- Publication Date:
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1401723
- Resource Type:
- Publisher's Accepted Manuscript
- Journal Name:
- The Plant Journal
- Additional Journal Information:
- Journal Name: The Plant Journal Journal Volume: 89 Journal Issue: 4; Journal ID: ISSN 0960-7412
- Publisher:
- Wiley-Blackwell
- Country of Publication:
- United Kingdom
- Language:
- English
Citation Formats
McGregor, Nicholas, Yin, Victor, Tung, Ching‐Chieh, Van Petegem, Filip, and Brumer, Harry. Crystallographic insight into the evolutionary origins of xyloglucan endotransglycosylases and endohydrolases. United Kingdom: N. p., 2017.
Web. doi:10.1111/tpj.13421.
McGregor, Nicholas, Yin, Victor, Tung, Ching‐Chieh, Van Petegem, Filip, & Brumer, Harry. Crystallographic insight into the evolutionary origins of xyloglucan endotransglycosylases and endohydrolases. United Kingdom. https://doi.org/10.1111/tpj.13421
McGregor, Nicholas, Yin, Victor, Tung, Ching‐Chieh, Van Petegem, Filip, and Brumer, Harry. Sat .
"Crystallographic insight into the evolutionary origins of xyloglucan endotransglycosylases and endohydrolases". United Kingdom. https://doi.org/10.1111/tpj.13421.
@article{osti_1401723,
title = {Crystallographic insight into the evolutionary origins of xyloglucan endotransglycosylases and endohydrolases},
author = {McGregor, Nicholas and Yin, Victor and Tung, Ching‐Chieh and Van Petegem, Filip and Brumer, Harry},
abstractNote = {Summary The xyloglucan endotransglycosylase/hydrolase ( XTH ) gene family encodes enzymes of central importance to plant cell wall remodeling. The evolutionary history of plant XTH gene products is incompletely understood vis‐à‐vis the larger body of bacterial endoglycanases in Glycoside Hydrolase Family 16 ( GH 16). To provide molecular insight into this issue, high‐resolution X‐ray crystal structures and detailed enzyme kinetics of an extant transitional plant endoglucanase ( EG ) were determined. Functionally intermediate between plant XTH gene products and bacterial licheninases of GH 16, Vitis vinifera EG 16 (Vv EG 16) effectively catalyzes the hydrolysis of the backbones of two dominant plant cell wall matrix glycans, xyloglucan (XyG) and β(1,3)/β(1,4)‐mixed‐linkage glucan ( MLG ). Crystallographic complexes with extended oligosaccharide substrates reveal the structural basis for the accommodation of both unbranched, mixed‐linked ( MLG ) and highly decorated, linear (XyG) polysaccharide chains in a broad, extended active‐site cleft. Structural comparison with representative bacterial licheninases, a xyloglucan endotranglycosylase ( XET ), and a xyloglucan endohydrolase ( XEH ) outline the functional ramifications of key sequence deletions and insertions across the phylogenetic landscape of GH 16. Although the biological role(s) of EG 16 orthologs remains to be fully resolved, the present biochemical and tertiary structural characterization provides key insight into plant cell wall enzyme evolution, which will continue to inform genomic analyses and functional studies across species.},
doi = {10.1111/tpj.13421},
journal = {The Plant Journal},
number = 4,
volume = 89,
place = {United Kingdom},
year = {Sat Feb 11 00:00:00 EST 2017},
month = {Sat Feb 11 00:00:00 EST 2017}
}
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Works referenced in this record:
CHAINSAW : a program for mutating pdb files used as templates in molecular replacement
journal, April 2008
- Stein, Norman
- Journal of Applied Crystallography, Vol. 41, Issue 3
Biological implications of the occurrence of 32 members of the XTH (xyloglucan endotransglucosylase/hydrolase) family of proteins in the bryophyte Physcomitrella patens: XTH family of genes in Physcomitrella patens
journal, October 2010
- Yokoyama, Ryusuke; Uwagaki, Yohei; Sasaki, Hiroki
- The Plant Journal, Vol. 64, Issue 4
Hemicellulose biosynthesis
journal, June 2013
- Pauly, Markus; Gille, Sascha; Liu, Lifeng
- Planta, Vol. 238, Issue 4
Mixed-linkage (1→3,1→4)-β-d-glucan is a major hemicellulose of Equisetum (horsetail) cell walls
journal, July 2008
- Fry, Stephen C.; Nesselrode, Bertram H. W. A.; Miller, Janice G.
- New Phytologist, Vol. 179, Issue 1
Refinement of Macromolecular Structures by the Maximum-Likelihood Method
journal, May 1997
- Murshudov, G. N.; Vagin, A. A.; Dodson, E. J.
- Acta Crystallographica Section D Biological Crystallography, Vol. 53, Issue 3
Hemicelluloses
journal, June 2010
- Scheller, Henrik Vibe; Ulvskov, Peter
- Annual Review of Plant Biology, Vol. 61, Issue 1
Overview of the CCP 4 suite and current developments
journal, March 2011
- Winn, Martyn D.; Ballard, Charles C.; Cowtan, Kevin D.
- Acta Crystallographica Section D Biological Crystallography, Vol. 67, Issue 4
Biochemical and Structural Characterization of the Complex Agarolytic Enzyme System from the Marine Bacterium Zobellia galactanivorans
journal, July 2012
- Hehemann, Jan-Hendrik; Correc, Gaëlle; Thomas, François
- Journal of Biological Chemistry, Vol. 287, Issue 36
AtBGAL10 Is the Main Xyloglucan β-Galactosidase in Arabidopsis, and Its Absence Results in Unusual Xyloglucan Subunits and Growth Defects
journal, January 2012
- Sampedro, Javier; Gianzo, Cristina; Iglesias, Natalia
- Plant Physiology, Vol. 158, Issue 3
Plant glycosyl hydrolases and biofuels: a natural marriage
journal, June 2008
- Lopezcasado, G.; Urbanowicz, B.; Damasceno, C.
- Current Opinion in Plant Biology, Vol. 11, Issue 3
Kinetic Analyses of Retaining endo -(Xylo)glucanases from Plant and Microbial Sources Using New Chromogenic Xylogluco-Oligosaccharide Aryl Glycosides †
journal, July 2008
- Ibatullin, Farid M.; Baumann, Martin J.; Greffe, Lionel
- Biochemistry, Vol. 47, Issue 29
Structural Basis for the Substrate Specificity of a Bacillus 1,3-1,4-β-Glucanase
journal, April 2006
- Gaiser, Olaf J.; Piotukh, Kirill; Ponnuswamy, Mondikalipudur N.
- Journal of Molecular Biology, Vol. 357, Issue 4
Exploring the evolution of (1,3;1,4)-β-d-glucans in plant cell walls: comparative genomics can help!
journal, April 2009
- Fincher, Geoffrey B.
- Current Opinion in Plant Biology, Vol. 12, Issue 2
Straying off the Highway: Trafficking of Secreted Plant Proteins and Complexity in the Plant Cell Wall Proteome
journal, March 2010
- Rose, Jocelyn K. C.; Lee, Sang-Jik
- Plant Physiology, Vol. 153, Issue 2
The 1.3 Å crystal structure of a novel endo-β-1,3-glucanase of glycoside hydrolase family 16 from alkaliphilic Nocardiopsis sp. strain F96
journal, August 2007
- Fibriansah, Guntur; Masuda, Sumiko; Koizumi, Naoya
- Proteins: Structure, Function, and Bioinformatics, Vol. 69, Issue 3
REFMAC 5 dictionary: organization of prior chemical knowledge and guidelines for its use
journal, November 2004
- Vagin, Alexei A.; Steiner, Roberto A.; Lebedev, Andrey A.
- Acta Crystallographica Section D Biological Crystallography, Vol. 60, Issue 12
Hetero-trans-β-glucanase, an enzyme unique to Equisetum plants, functionalizes cellulose
journal, August 2015
- Simmons, Thomas J.; Mohler, Kyle E.; Holland, Claire
- The Plant Journal, Vol. 83, Issue 5
Characterization and Three-dimensional Structures of Two Distinct Bacterial Xyloglucanases from Families GH5 and GH12
journal, March 2007
- Gloster, Tracey M.; Ibatullin, Farid M.; Macauley, Katherine
- Journal of Biological Chemistry, Vol. 282, Issue 26
The molecular cloning and expression of a cellobiase gene from anAgrobacterium inEscherichia coli
journal, October 1986
- Wakarchuk, Warren W.; Kilburn, Douglas G.; Miller, Robert C.
- MGG Molecular & General Genetics, Vol. 205, Issue 1
Towards automated crystallographic structure refinement with phenix.refine
journal, March 2012
- Afonine, Pavel V.; Grosse-Kunstleve, Ralf W.; Echols, Nathaniel
- Acta Crystallographica Section D Biological Crystallography, Vol. 68, Issue 4
Structural analysis of xyloglucan oligosaccharides by 1H-n.m.r. spectroscopy and fast-atom-bombardment mass spectrometry
journal, April 1990
- York, William S.; van Halbeek, Herman; Darvill, Alan G.
- Carbohydrate Research, Vol. 200
Recent structural insights into the enzymology of the ubiquitous plant cell wall glycan xyloglucan
journal, October 2016
- Attia, Mohamed A.; Brumer, Harry
- Current Opinion in Structural Biology, Vol. 40
Stereochemical course and structure of the products of the enzymic action of endo-1,3-1,4-β-d-glucan 4-glucanohydrolase from Bacillus licheniformis
journal, December 1993
- Malet, C.; Jiménez-Barbero, J.; Bernabé, M.
- Biochemical Journal, Vol. 296, Issue 3
Analysis of Xyloglucan Endotransglycosylase/Hydrolase (XTH) Genes and Diverse Roles of Isoenzymes during Persimmon Fruit Development and Postharvest Softening
journal, April 2015
- Han, Ye; Zhu, Qinggang; Zhang, Zhengke
- PLOS ONE, Vol. 10, Issue 4
The kappa-carrageenase of the marine bacterium Cytophaga drobachiensis. Structural and phylogenetic relationships within family-16 glycoside hydrolases
journal, May 1998
- Barbeyron, T.; Gerard, A.; Potin, P.
- Molecular Biology and Evolution, Vol. 15, Issue 5
Xyloglucan Endotransglycosylases Have a Function during the Formation of Secondary Cell Walls of Vascular Tissues
journal, November 2002
- Bourquin, Veronica; Nishikubo, Nobuyuki; Abe, Hisashi
- The Plant Cell, Vol. 14, Issue 12
Analysis of nasturtium Tm NXG1 complexes by crystallography and molecular dynamics provides detailed insight into substrate recognition by family GH16 xyloglucan endo -transglycosylases and endo -hydrolases
journal, June 2009
- Mark, Pekka; Baumann, Martin J.; Eklöf, Jens M.
- Proteins: Structure, Function, and Bioinformatics, Vol. 75, Issue 4
Development of a citrus genome-wide EST collection and cDNA microarray as resources for genomic studies
journal, February 2005
- Forment, J.; Gadea, J.; Huerta, L.
- Plant Molecular Biology, Vol. 57, Issue 3
Biochemistry and physiological roles of enzymes that ‘cut and paste’ plant cell-wall polysaccharides
journal, August 2013
- Franková, Lenka; Fry, Stephen C.
- Journal of Experimental Botany, Vol. 64, Issue 12
Endo-(1,4)-β-Glucanase gene families in the grasses: temporal and spatial Co-transcription of orthologous genes1
journal, January 2012
- Buchanan, Margaret; Burton, Rachel A.; Dhugga, Kanwarpal S.
- BMC Plant Biology, Vol. 12, Issue 1
ScanProsite: detection of PROSITE signature matches and ProRule-associated functional and structural residues in proteins
journal, July 2006
- de Castro, E.; Sigrist, C. J. A.; Gattiker, A.
- Nucleic Acids Research, Vol. 34, Issue Web Server
Phytozome: a comparative platform for green plant genomics
journal, November 2011
- Goodstein, David M.; Shu, Shengqiang; Howson, Russell
- Nucleic Acids Research, Vol. 40, Issue D1
Synthesis and Analysis of Specific Covalent Inhibitors of endo -Xyloglucanases
journal, February 2015
- Fenger, Thomas Hauch; Brumer, Harry
- ChemBioChem, Vol. 16, Issue 4
Annotation and comparative analysis of the glycoside hydrolase genes in Brachypodium distachyon
journal, January 2010
- Tyler, Ludmila; Bragg, Jennifer N.; Wu, Jiajie
- BMC Genomics, Vol. 11, Article No. 600
Hydrolytic Activity and Substrate Specificity of an Endoglucanase from Zea mays Seedling Cell Walls
journal, January 1987
- Hatfield, Ronald D.; Nevins, Donald J.
- Plant Physiology, Vol. 83, Issue 1
XDS
journal, January 2010
- Kabsch, Wolfgang
- Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 2
Stereochemistry and the Mechanism of Enzymatic Reactions
journal, November 1953
- Koshland, D. E.
- Biological Reviews, Vol. 28, Issue 4
The XTH Gene Family: An Update on Enzyme Structure, Function, and Phylogeny in Xyloglucan Remodeling
journal, April 2010
- Eklöf, Jens M.; Brumer, Harry
- Plant Physiology, Vol. 153, Issue 2
Enzymatic assembly of DNA molecules up to several hundred kilobases
journal, April 2009
- Gibson, Daniel G.; Young, Lei; Chuang, Ray-Yuan
- Nature Methods, Vol. 6, Issue 5, p. 343-345
Heat stress affects flowering, berry growth, sugar accumulation and photosynthesis of Vitis vinifera cv. Semillon grapevines grown in a controlled environment
journal, January 2010
- Greer, Dennis H.; Weston, Chris
- Functional Plant Biology, Vol. 37, Issue 3
Molecular dynamics simulations of a branched tetradecasaccharide substrate in the active site of a xyloglucan endo -transglycosylase
journal, October 2011
- Mark, Pekka; Zhang, Qiong; Czjzek, Mirjam
- Molecular Simulation, Vol. 37, Issue 12
Structural and enzymatic characterization of a glycoside hydrolase family 31 α-xylosidase from Cellvibrio japonicus involved in xyloglucan saccharification
journal, May 2011
- Larsbrink, Johan; Izumi, Atsushi; Ibatullin, Farid M.
- Biochemical Journal, Vol. 436, Issue 3
Incorporation of Prior Phase Information Strengthens Maximum-Likelihood Structure Refinement
journal, November 1998
- Pannu, Navraj S.; Murshudov, Garib N.; Dodson, Eleanor J.
- Acta Crystallographica Section D Biological Crystallography, Vol. 54, Issue 6
Differences in enzymic properties of five recombinant xyloglucan endotransglucosylase/hydrolase (XTH) proteins of Arabidopsis thaliana
journal, August 2010
- Maris, An; Kaewthai, Nomchit; Eklöf, Jens M.
- Journal of Experimental Botany, Vol. 62, Issue 1
The β-Glucanase Zg LamA from Zobellia galactanivorans Evolved a Bent Active Site Adapted for Efficient Degradation of Algal Laminarin
journal, December 2013
- Labourel, Aurore; Jam, Murielle; Jeudy, Alexandra
- Journal of Biological Chemistry, Vol. 289, Issue 4
A general, robust method for the quality control of intact proteins using LC–ESI-MS
journal, June 2007
- Sundqvist, Gustav; Stenvall, Maria; Berglund, Helena
- Journal of Chromatography B, Vol. 852, Issue 1-2
Biosynthesis of the Plant Cell Wall Matrix Polysaccharide Xyloglucan
journal, April 2016
- Pauly, Markus; Keegstra, Kenneth
- Annual Review of Plant Biology, Vol. 67, Issue 1
Privateer: software for the conformational validation of carbohydrate structures
journal, November 2015
- Agirre, Jon; Iglesias-Fernández, Javier; Rovira, Carme
- Nature Structural & Molecular Biology, Vol. 22, Issue 11
Structural Evidence for the Evolution of Xyloglucanase Activity from Xyloglucan Endo -Transglycosylases: Biological Implications for Cell Wall Metabolism
journal, June 2007
- Baumann, Martin J.; Eklöf, Jens M.; Michel, Gurvan
- The Plant Cell, Vol. 19, Issue 6
Plant glycoside hydrolases involved in cell wall polysaccharide degradation
journal, July 2006
- Minic, Z.; Jouanin, L.
- Plant Physiology and Biochemistry, Vol. 44, Issue 7-9
Building Custom Polysaccharides in Vitro with an Efficient, Broad-Specificity Xyloglucan Glycosynthase and a Fucosyltransferase
journal, July 2011
- Spadiut, Oliver; Ibatullin, Farid M.; Peart, Jonelle
- Journal of the American Chemical Society, Vol. 133, Issue 28
Generation and structural validation of a library of diverse xyloglucan-derived oligosaccharides, including an update on xyloglucan nomenclature
journal, January 2015
- Tuomivaara, Sami T.; Yaoi, Katsuro; O’Neill, Malcolm A.
- Carbohydrate Research, Vol. 402
Cloning theClostridium thermocellum thermostable laminarinase gene inEscherichia coli: The properties of the enzyme thus produced
journal, November 1990
- Zverlov, V. V.; Velikodvorskaya, G. A.
- Biotechnology Letters, Vol. 12, Issue 11
MolProbity : all-atom structure validation for macromolecular crystallography
journal, December 2009
- Chen, Vincent B.; Arendall, W. Bryan; Headd, Jeffrey J.
- Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 1
Coot model-building tools for molecular graphics
journal, November 2004
- Emsley, Paul; Cowtan, Kevin
- Acta Crystallographica Section D Biological Crystallography, Vol. 60, Issue 12, p. 2126-2132
Cell Wall-Related Proteins of Unknown Function: Missing Links in Plant Cell Wall Development
journal, May 2014
- Mewalal, Ritesh; Mizrachi, Eshchar; Mansfield, Shawn D.
- Plant and Cell Physiology, Vol. 55, Issue 6
Deciphering key features in protein structures with the new ENDscript server
journal, April 2014
- Robert, Xavier; Gouet, Patrice
- Nucleic Acids Research, Vol. 42, Issue W1
The carbohydrate-active enzymes database (CAZy) in 2013
journal, November 2013
- Lombard, Vincent; Golaconda Ramulu, Hemalatha; Drula, Elodie
- Nucleic Acids Research, Vol. 42, Issue D1
Crystal structure of an IRF-DNA complex reveals novel DNA recognition and cooperative binding to a tandem repeat of core sequences
journal, September 1999
- Fujii, Y.
- The EMBO Journal, Vol. 18, Issue 18
Expresso: automatic incorporation of structural information in multiple sequence alignments using 3D-Coffee
journal, July 2006
- Armougom, F.; Moretti, S.; Poirot, O.
- Nucleic Acids Research, Vol. 34, Issue Web Server
Developmental Expression Patterns of Arabidopsis XTH Genes Reported by Transgenes and Genevestigator
journal, June 2006
- Becnel, Jaime; Natarajan, Mukil; Kipp, Alex
- Plant Molecular Biology, Vol. 61, Issue 3
Crystal Structures of a Poplar Xyloglucan Endotransglycosylase Reveal Details of Transglycosylation Acceptor Binding
journal, March 2004
- Johansson, Patrik; Brumer, Harry; Baumann, Martin J.
- The Plant Cell, Vol. 16, Issue 4
The structure of a chromosomal high mobility group protein-DNA complex reveals sequence-neutral mechanisms important for non-sequence-specific DNA recognition
journal, December 1999
- Murphy IV, F. V.
- The EMBO Journal, Vol. 18, Issue 23
Expression and Purification of Soluble His6-Tagged TEV Protease
book, January 2009
- Tropea, Joseph E.; Cherry, Scott; Waugh, David S.
- Methods in Molecular Biology
PHENIX: a comprehensive Python-based system for macromolecular structure solution
journal, January 2010
- Adams, Paul D.; Afonine, Pavel V.; Bunkóczi, Gábor
- Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 2, p. 213-221
Poplar Carbohydrate-Active Enzymes. Gene Identification and Expression Analyses
journal, January 2006
- Geisler-Lee, Jane; Geisler, Matt; Coutinho, Pedro M.
- Plant Physiology, Vol. 140, Issue 3
Function of xyloglucan endotransglucosylase/hydrolases in rice
journal, December 2013
- Hara, Yoshinao; Yokoyama, Ryusuke; Osakabe, Keishi
- Annals of Botany, Vol. 114, Issue 6
A Barley Xyloglucan Xyloglucosyl Transferase Covalently Links Xyloglucan, Cellulosic Substrates, and (1,3;1,4)-β-D-Glucans
journal, February 2007
- Hrmova, Maria; Farkas, Vladimir; Lahnstein, Jelle
- Journal of Biological Chemistry, Vol. 282, Issue 17
Structure-Function Analysis of a Mixed-linkage β-Glucanase/Xyloglucanase from the Key Ruminal Bacteroidetes Prevotella bryantii B 1 4
journal, October 2015
- McGregor, Nicholas; Morar, Mariya; Fenger, Thomas Hauch
- Journal of Biological Chemistry, Vol. 291, Issue 3
Glycosyltransferases and cell wall biosynthesis: novel players and insights
journal, June 2004
- Scheible, Wolf-Rüdiger; Pauly, Markus
- Current Opinion in Plant Biology, Vol. 7, Issue 3
Group III-A XTH Genes of Arabidopsis Encode Predominant Xyloglucan Endohydrolases That Are Dispensable for Normal Growth
journal, October 2012
- Kaewthai, Nomchit; Gendre, Delphine; Eklöf, Jens M.
- Plant Physiology, Vol. 161, Issue 1
The κ-carrageenase of P. carrageenovora Features a Tunnel-Shaped Active Site
journal, June 2001
- Michel, Gurvan; Chantalat, Laurent; Duee, Emile
- Structure, Vol. 9, Issue 6
Generation of ESTs in Vitis vinifera wine grape (Cabernet Sauvignon) and table grape (Muscat Hamburg) and discovery of new candidate genes with potential roles in berry development
journal, November 2007
- Peng, Fred Y.; Reid, Karen E.; Liao, Nancy
- Gene, Vol. 402, Issue 1-2
Hydrolysis of barley (1→3), (1→4)-β-d-glucan by a cellobiohydrolase II preparation from Trichoderma reesei
journal, January 1995
- Henriksson, Kirsti; Teleman, Anita; Suortti, Tapani
- Carbohydrate Polymers, Vol. 26, Issue 2
Heterogeneity in the chemistry, structure and function of plant cell walls
journal, September 2010
- Burton, Rachel A.; Gidley, Michael J.; Fincher, Geoffrey B.
- Nature Chemical Biology, Vol. 6, Issue 10
Functional genomics of cell elongation in developing cotton fibers
journal, April 2004
- Arpat, A.; Waugh, Mark; Sullivan, John P.
- Plant Molecular Biology, Vol. 54, Issue 6
Automated macromolecular model building for X-ray crystallography using ARP/wARP version 7
journal, June 2008
- Langer, Gerrit; Cohen, Serge X.; Lamzin, Victor S.
- Nature Protocols, Vol. 3, Issue 7
Xyloglucan endotransglycosylase/hydrolase genes in cotton and their role in fiber elongation
journal, August 2010
- Lee, Joohyun; Burns, Teresa H.; Light, Ginger
- Planta, Vol. 232, Issue 5
Nomenclature for sugar-binding subsites in glycosyl hydrolases
journal, January 1997
- Davies, Gideon J.; Wilson, Keith S.; Henrissat, Bernard
- Biochemical Journal, Vol. 321, Issue 2
Structure-Function Analysis of a Broad Specificity Populus trichocarpa Endo-β-glucanase Reveals an Evolutionary Link between Bacterial Licheninases and Plant XTH Gene Products
journal, April 2013
- Eklöf, Jens M.; Shojania, Shaheen; Okon, Mark
- Journal of Biological Chemistry, Vol. 288, Issue 22
X-ray crystal structures of Phanerochaete chrysosporium Laminarinase 16A in complex with products from lichenin and laminarin hydrolysis
journal, July 2009
- Vasur, Jonas; Kawai, Rie; Andersson, Evalena
- FEBS Journal, Vol. 276, Issue 14
Blu-Ice and the Distributed Control System : software for data acquisition and instrument control at macromolecular crystallography beamlines
journal, November 2002
- McPhillips, Timothy M.; McPhillips, Scott E.; Chiu, Hsiu-Ju
- Journal of Synchrotron Radiation, Vol. 9, Issue 6
MEGA6: Molecular Evolutionary Genetics Analysis Version 6.0
journal, October 2013
- Tamura, Koichiro; Stecher, Glen; Peterson, Daniel
- Molecular Biology and Evolution, Vol. 30, Issue 12
Bacterial 1,3-1,4-β-glucanases: structure, function and protein engineering
journal, December 2000
- Planas, Antoni
- Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, Vol. 1543, Issue 2
The XTH Family of Enzymes Involved in Xyloglucan Endotransglucosylation and Endohydrolysis: Current Perspectives and a New Unifying Nomenclature
journal, December 2002
- Rose, Jocelyn K. C.; Braam, Janet; Fry, Stephen C.
- Plant and Cell Physiology, Vol. 43, Issue 12
Phaser crystallographic software
journal, July 2007
- McCoy, Airlie J.; Grosse-Kunstleve, Ralf W.; Adams, Paul D.
- Journal of Applied Crystallography, Vol. 40, Issue 4
Evolution and divergence in the coding and promoter regions of the Populus gene family encoding xyloglucan endotransglycosylase/hydrolases
journal, September 2011
- Ye, Xia; Yuan, Suhua; Guo, Hong
- Tree Genetics & Genomes, Vol. 8, Issue 1
Crystal Structure of a Smad MH1 Domain Bound to DNA
journal, September 1998
- Shi, Yigong; Wang, Yan-Fei; Jayaraman, Lata
- Cell, Vol. 94, Issue 5