DOE PAGES title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Cell wall composition profiling of parasitic giant dodder ( C uscuta reflexa ) and its hosts: a priori differences and induced changes

Abstract

Summary Host plant penetration is the gateway to survival for holoparasitic Cuscuta and requires host cell wall degradation. Compositional differences of cell walls may explain why some hosts are amenable to such degradation while others can resist infection. Antibody‐based techniques for comprehensive profiling of cell wall epitopes and cell wall‐modifying enzymes were applied to several susceptible hosts and a resistant host of Cuscuta reflexa and to the parasite itself. Infected tissue of Pelargonium zonale contained high concentrations of de‐esterified homogalacturonans in the cell walls, particularly adjacent to the parasite's haustoria. High pectinolytic activity in haustorial extracts and high expression levels of pectate lyase genes suggest that the parasite contributes directly to wall remodeling. Mannan and xylan concentrations were low in P. zonale and in five susceptible tomato introgression lines, but high in the resistant Solanum lycopersicum cv M82, and in C. reflexa itself. Knowledge of the composition of resistant host cell walls and the parasite's own cell walls is useful in developing strategies to prevent infection by parasitic plants.

Authors:
 [1];  [1];  [1];  [2];  [2];  [2];  [3];  [1]
  1. Department of Arctic and Marine Biology Faculty of Biosciences Fisheries and Economics UiT The Arctic University of Norway 9037 Tromsø Norway
  2. Department of Plant and Environmental Sciences Faculty of Science University of Copenhagen 1871 Frederiksberg Denmark
  3. Department of Plant Biology Cornell University 412 Mann Library Building 14853 Ithaca NY USA
Publication Date:
Sponsoring Org.:
USDOE
OSTI Identifier:
1401613
Grant/Contract Number:  
DE‐SC0006645; EU-ITN 238084; IOS-1339887; SC0006645
Resource Type:
Publisher's Accepted Manuscript
Journal Name:
New Phytologist
Additional Journal Information:
Journal Name: New Phytologist Journal Volume: 207 Journal Issue: 3; Journal ID: ISSN 0028-646X
Publisher:
Wiley-Blackwell
Country of Publication:
United Kingdom
Language:
English

Citation Formats

Johnsen, Hanne R., Striberny, Bernd, Olsen, Stian, Vidal‐Melgosa, Silvia, Fangel, Jonatan U., Willats, William G. T., Rose, Jocelyn K. C., and Krause, Kirsten. Cell wall composition profiling of parasitic giant dodder ( C uscuta reflexa ) and its hosts: a priori differences and induced changes. United Kingdom: N. p., 2015. Web. doi:10.1111/nph.13378.
Johnsen, Hanne R., Striberny, Bernd, Olsen, Stian, Vidal‐Melgosa, Silvia, Fangel, Jonatan U., Willats, William G. T., Rose, Jocelyn K. C., & Krause, Kirsten. Cell wall composition profiling of parasitic giant dodder ( C uscuta reflexa ) and its hosts: a priori differences and induced changes. United Kingdom. https://doi.org/10.1111/nph.13378
Johnsen, Hanne R., Striberny, Bernd, Olsen, Stian, Vidal‐Melgosa, Silvia, Fangel, Jonatan U., Willats, William G. T., Rose, Jocelyn K. C., and Krause, Kirsten. Mon . "Cell wall composition profiling of parasitic giant dodder ( C uscuta reflexa ) and its hosts: a priori differences and induced changes". United Kingdom. https://doi.org/10.1111/nph.13378.
@article{osti_1401613,
title = {Cell wall composition profiling of parasitic giant dodder ( C uscuta reflexa ) and its hosts: a priori differences and induced changes},
author = {Johnsen, Hanne R. and Striberny, Bernd and Olsen, Stian and Vidal‐Melgosa, Silvia and Fangel, Jonatan U. and Willats, William G. T. and Rose, Jocelyn K. C. and Krause, Kirsten},
abstractNote = {Summary Host plant penetration is the gateway to survival for holoparasitic Cuscuta and requires host cell wall degradation. Compositional differences of cell walls may explain why some hosts are amenable to such degradation while others can resist infection. Antibody‐based techniques for comprehensive profiling of cell wall epitopes and cell wall‐modifying enzymes were applied to several susceptible hosts and a resistant host of Cuscuta reflexa and to the parasite itself. Infected tissue of Pelargonium zonale contained high concentrations of de‐esterified homogalacturonans in the cell walls, particularly adjacent to the parasite's haustoria. High pectinolytic activity in haustorial extracts and high expression levels of pectate lyase genes suggest that the parasite contributes directly to wall remodeling. Mannan and xylan concentrations were low in P. zonale and in five susceptible tomato introgression lines, but high in the resistant Solanum lycopersicum cv M82, and in C. reflexa itself. Knowledge of the composition of resistant host cell walls and the parasite's own cell walls is useful in developing strategies to prevent infection by parasitic plants.},
doi = {10.1111/nph.13378},
journal = {New Phytologist},
number = 3,
volume = 207,
place = {United Kingdom},
year = {Mon Mar 23 00:00:00 EDT 2015},
month = {Mon Mar 23 00:00:00 EDT 2015}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
https://doi.org/10.1111/nph.13378

Citation Metrics:
Cited by: 45 works
Citation information provided by
Web of Science

Save / Share:

Works referenced in this record:

An attack of the plant parasite Cuscuta reflexa induces the expression of attAGP , an attachment protein of the host tomato
journal, November 2006


Cellulase Activity Screening Using Pure Carboxymethylcellulose: Application to Soluble Cellulolytic Samples and to Plant Tissue Prints
journal, January 2014

  • Johnsen, Hanne; Krause, Kirsten
  • International Journal of Molecular Sciences, Vol. 15, Issue 1
  • DOI: 10.3390/ijms15010830

New insights into pectin methylesterase structure and function
journal, June 2007


Plant Cell Wall–Degrading Enzymes and Their Secretion in Plant-Pathogenic Fungi
journal, August 2014


Pectins as mediators of wall porosity in soybean cells
journal, September 1988

  • Baron-Epel, Orna; Gharyal, Paramjit K.; Schindler, Melvin
  • Planta, Vol. 175, Issue 3
  • DOI: 10.1007/BF00396345

Pectin: cell biology and prospects for functional analysis
journal, September 2001

  • Willats, William G. T.; McCartney, Lesley; Mackie, William
  • Plant Molecular Biology, Vol. 47, Issue 1/2, p. 9-27
  • DOI: 10.1023/A:1010662911148

Hemicelluloses
journal, June 2010


Xylogalacturonan exists in cell walls from various tissues of Arabidopsis thaliana
journal, April 2007


Development of Cuscuta species on a partially incompatible host: induction of xylem transfer cells
journal, March 2003


Growth of the plant cell wall
journal, November 2005

  • Cosgrove, Daniel J.
  • Nature Reviews Molecular Cell Biology, Vol. 6, Issue 11, p. 850-861
  • DOI: 10.1038/nrm1746

Profiling the main cell wall polysaccharides of tobacco leaves using high-throughput and fractionation techniques
journal, April 2012


Carbohydrate-binding modules promote the enzymatic deconstruction of intact plant cell walls by targeting and proximity effects
journal, August 2010

  • Herve, C.; Rogowski, A.; Blake, A. W.
  • Proceedings of the National Academy of Sciences, Vol. 107, Issue 34
  • DOI: 10.1073/pnas.1005732107

Cell wall integrity signaling and innate immunity in plants
journal, January 2012


A New Versatile Microarray-based Method for High Throughput Screening of Carbohydrate-active Enzymes
journal, February 2015

  • Vidal-Melgosa, Silvia; Pedersen, Henriette L.; Schückel, Julia
  • Journal of Biological Chemistry, Vol. 290, Issue 14
  • DOI: 10.1074/jbc.M114.630673

Arabinogalactan protein-rich cell walls, paramural deposits and ergastic globules define the hyaline bodies of rhinanthoid Orobanchaceae haustoria
journal, July 2014

  • Pielach, Anna; Leroux, Olivier; Domozych, David S.
  • Annals of Botany, Vol. 114, Issue 6
  • DOI: 10.1093/aob/mcu121

A xylogalacturonan epitope is specifically associated with plant cell detachment
journal, November 2003


High-throughput screening of Erwinia chrysanthemi pectin methylesterase variants using carbohydrate microarrays
journal, April 2009


The structure, function, and biosynthesis of plant cell wall pectic polysaccharides
journal, September 2009


Attachment of the parasitic weed dodder to the host
journal, May 2002


Invasion of Coffea arabica (Linn.) by Cuscuta jalapensis (Schlecht): in situ activity of peroxidase
journal, June 2006

  • López-Curto, L.; Márquez-Guzmán, J.; Díaz-Pontones, D. M.
  • Environmental and Experimental Botany, Vol. 56, Issue 2
  • DOI: 10.1016/j.envexpbot.2005.02.002

Cell Wall Degrading Enzymes in Cuscuta reflexa and Its Hosts
journal, January 1984

  • Nagar, Ranjana; Singh, M.; Sanwal, G. G.
  • Journal of Experimental Botany, Vol. 35, Issue 8
  • DOI: 10.1093/jxb/35.8.1104

Multiple forms of pectin methylesterase from Cuscuta reflexa filaments
journal, November 1994


A new mathematical model for relative quantification in real-time RT-PCR
journal, May 2001


The evolution of parasitism in plants
journal, April 2010


Strangers in the matrix: plant cell walls and pathogen susceptibility
journal, November 2008


An array of possibilities for pectin
journal, September 2009

  • Sørensen, Iben; Pedersen, Henriette Lodberg; Willats, William G. T.
  • Carbohydrate Research, Vol. 344, Issue 14
  • DOI: 10.1016/j.carres.2008.12.008

Influence of the Degree of Polymerization of Oligogalacturonates and of Esterification Pattern of Pectin on Their Recognition by Monoclonal Antibodies
journal, July 1992

  • Liners, Françoise; Thibault, Jean-François; Van Cutsem, Pierre
  • Plant Physiology, Vol. 99, Issue 3
  • DOI: 10.1104/pp.99.3.1099

Transfer of phloem-mobile substances from the host plants to the holoparasite Cuscuta sp.
journal, February 2006

  • Birschwilks, Mandy; Haupt, Sophie; Hofius, Daniel
  • Journal of Experimental Botany, Vol. 57, Issue 4
  • DOI: 10.1093/jxb/erj076

A Modified Hot Borate Method Significantly Enhances the Yield of High-Quality RNA from Cotton (Gossypium hirsutum L.)
journal, November 1994


Cell wall arabinan is essential for guard cell function
journal, September 2003

  • Jones, L.; Milne, J. L.; Ashford, D.
  • Proceedings of the National Academy of Sciences, Vol. 100, Issue 20
  • DOI: 10.1073/pnas.1832434100

The carbohydrate-active enzymes database (CAZy) in 2013
journal, November 2013

  • Lombard, Vincent; Golaconda Ramulu, Hemalatha; Drula, Elodie
  • Nucleic Acids Research, Vol. 42, Issue D1
  • DOI: 10.1093/nar/gkt1178

Pathogenesis by fungi and by parasitic plants: Similarities and differences
journal, February 2006


Culture of pectin methylesterase and polyphenoloxidasein Cuscuta campestris
journal, March 1999


Expression of arabinogalactan protein genes in pollen tubes of Arabidopsis thaliana
journal, October 2005


Altered Middle Lamella Homogalacturonan and Disrupted Deposition of (1→5)-α-l-Arabinan in the Pericarp of Cnr , a Ripening Mutant of Tomato
journal, May 2001

  • Orfila, Caroline; Seymour, Graham B.; Willats, William G. T.
  • Plant Physiology, Vol. 126, Issue 1
  • DOI: 10.1104/pp.126.1.210

Pectic homogalacturonan masks abundant sets of xyloglucan epitopes in plant cell walls
journal, January 2008

  • Marcus, Susan E.; Verhertbruggen, Yves; Hervé, Cécile
  • BMC Plant Biology, Vol. 8, Issue 1
  • DOI: 10.1186/1471-2229-8-60

Cuscuta spp: “Parasitic Plants in the Spotlight of Plant Physiology, Economy and Ecology”
book, January 2008


Biochemical and Immunocytological Characterizations of Arabidopsis Pollen Tube Cell Wall
journal, June 2010

  • Dardelle, Flavien; Lehner, Arnaud; Ramdani, Yasmina
  • Plant Physiology, Vol. 153, Issue 4
  • DOI: 10.1104/pp.110.158881

A cofactor requirement for polygalacturonase from Cuscuta campestris
journal, December 1999


Dodder hyphae invade the host: a structural and immunocytochemical characterization
journal, March 2003


Architecture of intracellular networks in plant matrices
journal, January 2009


Enzymatic treatments reveal differential capacities for xylan recognition and degradation in primary and secondary plant cell walls
journal, May 2009