Sexual reproduction, sporophyte development and molecular variation in the model moss Physcomitrella patens : introducing the ecotype Reute
Abstract
Summary Rich ecotype collections are used for several plant models to unravel the molecular causes of phenotypic differences, and to investigate the effects of environmental adaption and acclimation. For the model moss Physcomitrella patens collections of accessions are available, and have been used for phylogenetic and taxonomic studies, for example, but few have been investigated further for phenotypic differences. Here, we focus on the Reute accession and provide expression profiling and comparative developmental data for several stages of sporophyte development, as well as information on genetic variation via genomic sequencing. We analysed cross‐technology and cross‐laboratory data to define a confident set of 15 mature sporophyte‐specific genes. We find that the standard laboratory strain Gransden produces fewer sporophytes than Reute or Villersexel, although gametangia develop with the same time course and do not show evident morphological differences. Reute exhibits less genetic variation relative to Gransden than Villersexel, yet we found variation between Gransden and Reute in the expression profiles of several genes, as well as variation hot spots and genes that appear to evolve under positive Darwinian selection. We analyzed expression differences between the ecotypes for selected candidate genes in the GRAS transcription factor family, the chalcone synthase family and inmore »
- Authors:
-
- Plant Cell Biology Faculty of Biology University of Marburg Karl‐von‐Frisch‐Str. 8 35043 Marburg Germany
- Plant Cell Biology Faculty of Biology University of Marburg Karl‐von‐Frisch‐Str. 8 35043 Marburg Germany, BIOSS Centre for Biological Signaling Studies University of Freiburg Freiburg Germany
- Publication Date:
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1401515
- Grant/Contract Number:
- DE‐AC02‐05CH11231
- Resource Type:
- Publisher's Accepted Manuscript
- Journal Name:
- The Plant Journal
- Additional Journal Information:
- Journal Name: The Plant Journal Journal Volume: 90 Journal Issue: 3; Journal ID: ISSN 0960-7412
- Publisher:
- Wiley-Blackwell
- Country of Publication:
- United Kingdom
- Language:
- English
Citation Formats
Hiss, Manuel, Meyberg, Rabea, Westermann, Jens, Haas, Fabian B., Schneider, Lucas, Schallenberg‐Rüdinger, Mareike, Ullrich, Kristian K., and Rensing, Stefan A. Sexual reproduction, sporophyte development and molecular variation in the model moss Physcomitrella patens : introducing the ecotype Reute. United Kingdom: N. p., 2017.
Web. doi:10.1111/tpj.13501.
Hiss, Manuel, Meyberg, Rabea, Westermann, Jens, Haas, Fabian B., Schneider, Lucas, Schallenberg‐Rüdinger, Mareike, Ullrich, Kristian K., & Rensing, Stefan A. Sexual reproduction, sporophyte development and molecular variation in the model moss Physcomitrella patens : introducing the ecotype Reute. United Kingdom. https://doi.org/10.1111/tpj.13501
Hiss, Manuel, Meyberg, Rabea, Westermann, Jens, Haas, Fabian B., Schneider, Lucas, Schallenberg‐Rüdinger, Mareike, Ullrich, Kristian K., and Rensing, Stefan A. Sat .
"Sexual reproduction, sporophyte development and molecular variation in the model moss Physcomitrella patens : introducing the ecotype Reute". United Kingdom. https://doi.org/10.1111/tpj.13501.
@article{osti_1401515,
title = {Sexual reproduction, sporophyte development and molecular variation in the model moss Physcomitrella patens : introducing the ecotype Reute},
author = {Hiss, Manuel and Meyberg, Rabea and Westermann, Jens and Haas, Fabian B. and Schneider, Lucas and Schallenberg‐Rüdinger, Mareike and Ullrich, Kristian K. and Rensing, Stefan A.},
abstractNote = {Summary Rich ecotype collections are used for several plant models to unravel the molecular causes of phenotypic differences, and to investigate the effects of environmental adaption and acclimation. For the model moss Physcomitrella patens collections of accessions are available, and have been used for phylogenetic and taxonomic studies, for example, but few have been investigated further for phenotypic differences. Here, we focus on the Reute accession and provide expression profiling and comparative developmental data for several stages of sporophyte development, as well as information on genetic variation via genomic sequencing. We analysed cross‐technology and cross‐laboratory data to define a confident set of 15 mature sporophyte‐specific genes. We find that the standard laboratory strain Gransden produces fewer sporophytes than Reute or Villersexel, although gametangia develop with the same time course and do not show evident morphological differences. Reute exhibits less genetic variation relative to Gransden than Villersexel, yet we found variation between Gransden and Reute in the expression profiles of several genes, as well as variation hot spots and genes that appear to evolve under positive Darwinian selection. We analyzed expression differences between the ecotypes for selected candidate genes in the GRAS transcription factor family, the chalcone synthase family and in genes involved in cell wall modification that are potentially related to phenotypic differences. We confirm that Reute is a P. patens ecotype, and suggest its use for reverse‐genetics studies that involve progression through the life cycle and multiple generations.},
doi = {10.1111/tpj.13501},
journal = {The Plant Journal},
number = 3,
volume = 90,
place = {United Kingdom},
year = {Sat Mar 25 00:00:00 EDT 2017},
month = {Sat Mar 25 00:00:00 EDT 2017}
}
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